Antimicrobial resistance in commensal faecal Escherichia coli of hospitalised horses

The objective of this study was to examine the impact of hospitalisation and antimicrobial drug administration on the prevalence of resistance in commensal faecal E. coli of horses. Faecal samples were collected from ten hospitalised horses treated with antimicrobials, ten hospitalised horses not treated with antimicrobials and nine non-hospitalised horses over a consecutive five day period and susceptibility testing was performed on isolated E. coli. Results revealed that hospitalisation alone was associated with increased prevalence of antimicrobial resistance and multidrug resistance in commensal E. coli of horses. Due to the risk of transfer of resistance between commensal and pathogenic bacteria, veterinarians need to be aware of possible resistance in commensal bacteria when treating hospitalised horses

Low-Frequency High-Magnitude Mechanical Strain of Articular Chondrocytes Activates p38 MAPK and Induces Phenotypic Changes Associated with Osteoarthritis and Pain

Osteoarthritis (OA) is a debilitating joint disorder resulting from an incompletely understood combination of mechanical, biological, and biochemical processes. OA is often accompanied by inflammation and pain, whereby cytokines associated with chronic OA can up-regulate expression of neurotrophic factors such as nerve growth factor (NGF). Several studies suggest a role for cytokines and NGF in OA pain, however the effects of changing mechanical properties in OA tissue on chondrocyte metabolism remain unclear. Here, we used high-extension silicone rubber membranes to examine if high mechanical strain (HMS) of primary articular chondrocytes increases inflammatory gene expression and promotes neurotrophic factor release. HMS cultured chondrocytes displayed up-regulated NGF, TNFα and ADAMTS4 gene expression while decreasing TLR2 expression, as compared to static controls. HMS culture increased p38 MAPK activity compared to static controls. Conditioned medium from HMS dynamic cultures, but not static cultures, induced significant neurite sprouting in PC12 cells. The increased neurite sprouting was accompanied by consistent increases in PC12 cell death. Low-frequency high-magnitude mechanical strain of primary articular chondrocytes in vitro drives factor secretion associated with degenerative joint disease and joint pain. This study provides evidence for a direct link between cellular strain, secretory factors, neo-innervation, and pain in OA pathology

Molecular characterisation of a bovine-like rotavirus detected from a giraffe

Background Rotavirus (RV), is a member of the Reoviridae family and an important etiological agent of acute viral gastroenteritis in the young. Rotaviruses have a wide host range infecting a broad range of animal species, however little is known about rotavirus infection in exotic animals. In this paper we report the first characterisation of a RV strain from a giraffe calf. Results This report describes the identification and detailed molecular characterisation of a rotavirus strain detected from a 14-day-old Giraffe (Giraffa camelopardalis), presenting with acute diarrhea. The RV strain detected from the giraffe was characterized molecularly as G10P[11]. Detailed sequence analysis of VP4 and VP7 revealed significant identity at the amino acid sequence level to Bovine RV (BoRV). Conclusion This study demonstrates the need for continuous surveillance of RV strains in various animal populations, which will facilitate the identification of rotavirus hosts not previously reported. Furthermore, extending typical epidemiology studies to a broader host range will contribute to the timely identification of new emerging strain types

Influences of precipitation on water mass transformation and deep convection

Author Posting. © American Meteorological Society, 2012. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 42 (2012): 1684–1700, doi:10.1175/JPO-D-11-0230.1.The influences of precipitation on water mass transformation and the strength of the meridional overturning circulation in marginal seas are studied using theoretical and idealized numerical models. Nondimensional equations are developed for the temperature and salinity anomalies of deep convective water masses, making explicit their dependence on both geometric parameters such as basin area, sill depth, and latitude, as well as on the strength of atmospheric forcing. In addition to the properties of the convective water, the theory also predicts the magnitude of precipitation required to shut down deep convection and switch the circulation into the haline mode. High-resolution numerical model calculations compare well with the theory for the properties of the convective water mass, the strength of the meridional overturning circulation, and also the shutdown of deep convection. However, the numerical model also shows that, for precipitation levels that exceed this critical threshold, the circulation retains downwelling and northward heat transport, even in the absence of deep convection.This study was supported by the National Science Foundation underGrantsOCE-0850416, OCE-0959381, andOCE-0859381.2013-04-0

A 13-year record of erosion on badland sites in the Karoo, South Africa

Land degradation in South Africa has been of concern for more than 100 years with both climate change and inappropriate land management (overgrazing) being proposed as primary drivers. However, there are few quantitative studies of degradation and, in particular, few of erosion by water. Badlands, taken here to be the landform which results from extreme erosion, have been notably neglected. We report on 13 consecutive years of erosion pin measurements of badland erosion on 10 study sites in the Sneeuberg uplands of the eastern Karoo in South Africa. The study sites are on Holocene colluvium which mantles footslopes. They have been subject to overgrazing for at least 100 years, c. 1850–1950. Currently they are lightly grazed by sheep. The area receives about 500 mm rainfall per year. The sites are remote, with only informal, farmer-operated, daily raingauges nearby. The nearest subdaily raingauge is c. 55 km distant. Also we report on an analysis of the erosion pin data which focuses on establishing the origins and context of the badlands, including the relationship between study sites and adjacent valley-bottom gully systems; compare erosion rates on our study sites with rates determined by erosion pins on other badland sites; and discuss the implications of these erosion rates for landscape development and off-site impacts. Net erosion rates on the study sites are relatively high compared with global badland rates and range from 3.1 to 8.5 mm yr-1 which may be extrapolated to 53 to 145 t ha yr-1 (using a measured bulk density of 1.7 g cm-3). However, comparisons with badland sites elsewhere are difficult because of different measuring methodologies, lithologies, climate and dominant processes. Erosion rates on the study sites are strongly influenced by rainfall amounts and, in particular, by daily rainfall events which exceed ~10 mm: this is the threshold intensity at which runoff has been observed to commence on badlands. Of significance, but of lesser influence, is weathering, mainly by wetting and drying: this prepares bare surfaces for erosion. However, questions remain regarding the role of site characteristics, and of processes at each site, in determining between-site differences in erosion rate. Crude extrapolation of current rates of erosion, in conjunction with depths of incision into the badlands, suggests that badland development started around 200 years ago, probably as a response to the introduction of European-style stock farming which resulted in overgrazing. We assume, but cannot quantify, the additional influence of periods of drought and burning in the erosional history of the area. Intermittent connection of these badlands to valley-bottom gullies and therefore to small farm dams and ultimately to large water storage reservoirs increases their impact on local water resources