Research paper: Bilateral carotid artery occlusion and cochlear oxidative stress and hearing loss in rats

Introduction: This study aimed to evaluate the effects of bilateral carotid artery occlusion on cochlear oxidative stress and hearing status in rats. Methods: The rats were divided into two sets. The first set was used for electrophysiological recording (click and 4 kHz tone burst auditory brainstem responses and electrocochleography) on the day before surgery and then on the first, fourth, and seventh days after surgery. Animals of the second set were used for biochemical analysis. The cochlea of animals in the second set was collected on the first, fourth, and seventh days after carotids occlusion for biochemical analysis. For the control groups, no carotids occlusion was done. For ischemia induction, both common carotid arteries were occluded for 20 minutes. Results: Electrophysiological analysis showed that burst auditory brainstem thresholds significantly elevated after common carotid arteries occlusion on the first, fourth, and seventh days after surgery with abnormal electrocochleography results at 75, 70, and 85 on the first, fourth, and seventh days after surgery, respectively. The electrophysiological finding confirmed by biochemical results that showed malondialdehyde and nitric oxide levels increased and superoxide dismutase and catalase activities decreased after occlusion in cochlea tissue. Conclusion: This study showed that bilateral common carotid artery occlusion increases cochlear oxidative stress and induces hearing loss in rats. © 2020 Iran University of Medical Sciences. All rights reserved

Effect of Source Solution Components on Quality of Electrospun PVDF Nanofibers for Nanogenerator Application

High surface area flexible thin films of Polyvinylidene Difluoride (PVDF) nanofibers are vastly used because of low cost, simple, safety and environmentally friendly production methods. PVDF nanofibers are inherently piezoelectric when produced by high voltage electrospinning method. In this paper, PVDF nanofibers are synthesis in a electrospinning apparatus, using a solution that contains PVDF dissolved in two solvents, THF (Tetrahydroforan) and DMF (N,N-Di-Methyl Formamid), which DMF has a higher boiling point than THF. The effects of THF to DMF ratio in the solution, and also, PVDF concentration on the morphology and diameter of the synthesised nanofibers are studied. Field emission scanning electron microscopy analysis of the nanofiber thin films indicates that by adding THF in the source suspension, smoother and more uniform nanofibers with lower diameter are resulted. However, by further increasing THF ratio, the cone-jet mode is formed on the tip of the needle, rapidly dried, before reaching the collector substrate. Results of the further experiments indicate that decreasing of PVDF concentration in the deposition source solves the latter problem. On the other hand, by decreasing the THF ratio to DMF, the solution cannot completely be dried, before collecting by the substrate. The best and uniform nanofibers with the diameter of 200 to 300 nm are obtained from a ratio of 3:1 For THF:DMF and 10 % PVDF concentration. Fabricated nanogenerator, based on the best mentioned sample, shows an output power of 0.56 W/m2 when actuated by a vibration mechanical force with the frequency of 8 Hz

Monitoring of airborne asbestos fibers in an urban ambient air of Mashhad City, Iran: levels, spatial distribution and seasonal variations

Asbestos, as with other pollutants in the air, has adverse effects on the health of human beings and animals. Today, the relationship between presence of asbestos fibers in the air breathed by humans and developing serious diseases such as lung cancer (asbestosis) and mesothelioma has been proven. The objectives of this study were to monitor the levels of asbestos fibers in ambient air of Mashhad, Iran during 2018, and to draw its Geographic Information System (GIS) distribution map for the city. In this descriptive study, 13 sampling points in Mashhad city were chosen. Sampling of asbestos was carried out for 3 hour during summer and winter at 2018. Sampling of asbestos was performed using MCE (Mixed Cellulose Ester) membrane filters (pour size 0.45 µm; diameter: 25 mm) and cassette holder and peripheral pump. The samples were the analyzed by the phase contrast microscopy (PCM) method (NIOSH7400). Also, to investigate the type of asbestos and for more accurate counting of fibers, Scanning Electron Microscopy (SEM) analysis was utilized. Meteorological parameter were recorded through portable devices. To draw the graphs, Excel, R and Arc GIS software were used. Results showed that the mean asbestos fiber concentrations were equal to 11.40 ± 2.14 and 14.38 ± 2.52 f/L in summer and winter, respectively. The maximum level of asbestos fiber was detected in the station of Baitolmoghaddas square by 26.64 ± 2.14 and 19.3 SEM f/L in winter and summer, respectively. High concentration of asbestos fiber observed in this study can be attributed to the heavy traffic, the presence of prominent industries in the vicinity of the study area, and topographic features. The results from this research recommends that suitable controlling policies should be regulated to reduce both ambient air asbestos and its adverse health endpoints in Mashhad. © 2020, Springer Nature Switzerland AG

Development and implementation of water safety plans for groundwater resources in the southernmost city of West Azerbaijan Province, Iran

The transfer of water from the source to the consumption point is always associated with the possibility of contamination in any of its various components. To resolve this problem, the World Health Organization has considered a water safety plan. The purpose of this study is to implement water safety plan in the water supply system of Bukan city. This study was performed on Bukan’s water supply system in 2019–20 using a software to guarantee the quality of the water safety plan and the WHO and IWA guidelines. The software checklists were prepared and after confirming the validity of the translation and its facial and content validity, it was completed based on the records of the Water and Sewerage Company and interviews with experts. Out of a total of 440 points of full-application of the program and 392 points for the reviewed phases, 183.6 points were acquired and 43.7% of WSP-coordinated implementation was observed. The highest percentage of WSP-coordinated implementation (75.2%) was assigned to the validation stage with the highest point, and the support program stage had the lowest percentage of performance (1.1%). Among the major components of the water supply system, the final consumption point received the most attention from the system. Given the lifespan of the introduction and use of WSP in the world, it was expected that better results would be obtained from evaluating the implementation and progress of this approach in Bukan’s water supply system. However, the implementation rate of this program in this city compared to other cities in Iran, showed that according to the implementation time (one year), the obtained results are relatively convincing and good and the water supply system has a moderate level of safety

Distinct Regions of the Large Extracellular Domain of Tetraspanin CD9 Are Involved in the Control of Human Multinucleated Giant Cell Formation

Multinucleated giant cells, formed by the fusion of monocytes/macrophages, are features of chronic granulomatous inflammation associated with infections or the persistent presence of foreign material. The tetraspanins CD9 and CD81 regulate multinucleated giant cell formation: soluble recombinant proteins corresponding to the large extracellular domain (EC2) of human but not mouse CD9 can inhibit multinucleated giant cell formation, whereas human CD81 EC2 can antagonise this effect. Tetraspanin EC2 are all likely to have a conserved three helix sub-domain and a much less well-conserved or hypervariable sub-domain formed by short helices and interconnecting loops stabilised by two or more disulfide bridges. Using CD9/CD81 EC2 chimeras and point mutants we have mapped the specific regions of the CD9 EC2 involved in multinucleated giant cell formation. These were primarily located in two helices, one in each sub-domain. The cysteine residues involved in the formation of the disulfide bridges in CD9 EC2 were all essential for inhibitory activity but a conserved glycine residue in the tetraspanin-defining ‘CCG’ motif was not. A tyrosine residue in one of the active regions that is not conserved between human and mouse CD9 EC2, predicted to be solvent-exposed, was found to be only peripherally involved in this activity. We have defined two spatially-distinct sites on the CD9 EC2 that are required for inhibitory activity. Agents that target these sites could have therapeutic applications in diseases in which multinucleated giant cells play a pathogenic role

Modeling of Activated Carbon Preparation from Spanish Anthracite Based on ANFIS Structure

Carbon nanostructures are famous structures which are used in several industries such as separation, treatment, energy storage (i.e. methane and hydrogen storage), etc. A successful modeling of activated carbon preparation is very important in saving time and money. There are some attempts to achieve the appropriate theoretical modeling of activated carbon preparation but most of them were almost unsuccessful due to the complexity between the input and output variables. In this paper the empirical modeling of activated carbon preparation from Spanish anthracite based on adaptive neuro-fuzzy inference system (ANFIS) is investigated. ANFIS model is established to delineate the relationship between the BET surface area of the prepared activated carbon with initial and operational conditions; agent type, agent ratio, activation temperature, activation time and nitrogen flow. The results show that the selected model have a good accuracy with a coefficient of determination values (R2) of 0.9885 and average relative error (ARE) of 0.00268

Numerical Analysis of Inlet Gas-Mixture Flow Rate Effects on Carbon Nanotube Growth Rate

The growth rate and uniformity of Carbon Nano Tubes (CNTs) based on Chemical Vapor Deposition (CVD) technique is investigated by using a numerical model. In this reactor, inlet gas mixture, including xylene as carbon source and mixture of argon and hydrogen as  carrier gas enters into a horizontal CVD reactor at atmospheric pressure. Based on the gas phase and surface reactions, released carbon atoms are grown as CNTs on the iron catalysts at the reactor hot walls. The effect of inlet gas-mixture flow rate, on CNTs growth rate and its uniformity is discussed. In addition the velocity and temperature profile and also species concentrations throughout the reactor are presented

Numerical Study of Operating Pressure Effect on Carbon Nanotube Growth Rate and Length Uniformity

Chemical Vapor Deposition (CVD) is one of the most popular methods for producing Carbon Nanotubes (CNTs). The growth rate of CNTs based on CVD technique is investigated by using a numerical model based on finite volume method. Inlet gas mixture, including xylene as carbon source and mixture of argon and hydrogen as carrier gas enters into a horizontal CVD reactor at atmospheric pressure. In this article the operating pressure variations are studied as the effective parameter on CNT growth rate and length uniformity

Numerical Study of Wheat Conveying in Separator Cyclone using Computational Fluid Dynamics

IntroductionCyclones are widely used to separate solid particles from the fluid phase. Due to the ease of construction, low running costs, and hard-working conditions at high temperatures, people's interest in using cyclones is increasing day by day. Engineers are generally interested in two parameters to perform a complete evaluation of the design and operation of a cyclone. These parameters are the particle collecting efficiency and the pressure drop inside the cyclone. The precise prediction of the pressure drop in cyclone is very important which it is directly related to operating costs.Computational Fluid Dynamics (CFD) is a diversified tool for predicting flow behavior in a wide range of design and operational conditions. Numerical solution of Navier-Stokes equations is the basis of all CFD techniques, which is the result of fast computer upgrades and a better understanding of the numerical resolution of turbulence.Materials and MethodsRegarding preliminary experimental tests and understanding the fluid flow, the flow rate of 0.08 kg s-1 was selected as the flow rate. Six levels of inlet velocities 10, 12, 14, 16, 18, and 20 m s-1 were selected for understanding the effect of inlet velocity on the cyclone performance. The measurements were carried out using a hot-air anemometer (TSI-8484model with a resolution of 0.07 m s-1 and an operating range of 0.125 to 50 m s-1), and a pressure differential meter instrument (CPE310s-KIMO model) with an accuracy of 0.1 Pa.The region is discretized as a finite volume in a set, called the region grid or mesh after discretization. For incompressible fluids, pressure-based and density-based solvers are used, respectively. Regarding the velocity of the material entering the cyclone and low Mach number, a pressure-based solver could be used in this study.The shear stress transport model (SST) is a modified version of the k-ω 2-equation model. This model combines the two turbulence k-ω and k-ε models. The Lagrangian discrete phase model in Ansys Fluent follows to the Euler-Lagrangian model.Defining the best type of boundary condition is important for solving the problem and extracting solving fields. The boundary conditions used in this study include the inlet velocity in the entrance of cyclone and output pressure in both the upper and lower output sections.Results and DiscussionIn the results section, the results are initially validated by experimental results. Then, the parameters relating to separation efficiency and pressure drop are discussed. Finally, the tangential and axial velocities are considered as important parameters in the cyclone performance.One of the important issues in the cyclones is the static pressure because it completely affects the phenomenon of separation in the cyclone. The velocities of 16 m s-1 and 18 m s-1 have a good potential for use as the base velocity of the inlet fluid to the cyclone. The velocity of 20 m s-1 is not suitable for separation due to high-pressure drop related to high static pressure.The separation efficiency in the cyclone was 92 to 99% at all levels, the highest separation efficiency of 99% occurred at the velocity of 16 m s-1 and the lowest separation efficiency of 9% happened at the velocity of 20 m s-1.An increasing trend in axial and radial velocities occurred and the highest tangential velocity occurring in the input section. Considering the working conditions, the inlet velocities of 10 m s-1 to 16 m s-1 are appropriate for the turbulence intensity viewpoint.Conclusions(1): The speeds 16 m s-1 and 18 m s-1 showed a good potential for use as a base velocity of the fluid to the cyclone.(2): The highest separation efficiency for the velocity of 16 m s-1 (99%) and lower isolation efficiency was obtained at velocity of 20 m s-1 (92%).(3): The velocities of 10 m s-1 to 16 m s-1 are suitable input rates from the point of view of turbulence intensity.(4): It is concluded that from the point of view of wear to the velocity of 10 to 16 m s-1, practical use is possible, and the velocity of 18 m s-1 and 20 m s-1 require the reinforcement of the relevant sections