Coordinate Regulation of G- and C Strand Length during New Telomere Synthesis

We have used the ciliate Euplotes to study the role of DNA polymerase in telomeric C strand synthesis. Euplotes provides a unique opportunity to study C strand synthesis without the complication of simultaneous DNA replication because millions of new telomeres are made at a stage in the life cycle when no general DNA replication takes place. Previously we showed that the C-strands of newly synthesized telomeres have a precisely controlled length while the G-strands are more heterogeneous. This finding suggested that, although synthesis of the G-strand (by telomerase) is the first step in telomere addition, a major regulatory step occurs during subsequent C strand synthesis. We have now examined whether G- and C strand synthesis might be regulated coordinately rather than by two independent mechanisms. We accomplished this by determining what happens to G- and C strand length if C strand synthesis is partially inhibited by aphidicolin. Aphidicolin treatment caused a general lengthening of the G-strands and a large increase in C strand heterogeneity. This concomitant change in both the G- and C strand length indicates that synthesis of the two strands is coordinated. Since aphidicolin is a very specific inhibitor of DNA polα and polδ, our results suggest that this coordinate length regulation is mediated by DNA polymerase

Research on the Prewarning Method for the Safety of South-to-North Water Transfer Project Driven by Monitoring Data

In order to solve the prewarning problem of South-to-North Water Transfer Project safety, an intelligent cooperative prewarning method based on machine learning was proposed under the framework of intelligent information processing. Driven by the monitoring data of the South-to-North Water Transfer Project, the single sensor in typical scenes was studied, and the security threshold was predicted along the vertical axis of time, firstly. With the support of the data correlation calculation, the sensors in the typical scene were intelligently grouped, and the study objectives were changed into sensor grouping, secondly. Then, the nonlinear regression model between the single sensor and the multisensors was built on the time cross section, and the model was used to dynamically calculate the safety threshold of the current sensor for the second time. Finally, in the framework of intelligent information processing, a double verification mechanism was proposed to support the construction of the intelligent prewarning method for the safety of South-to-North Water Transfer Project. The paper collected the monitoring data from November 2015 to September 2016 in the typical scenarios. The experimental results showed that the methods constructed in the paper can be able to identify the abnormal causes of data sudden jump effectively and give the different level prewarning. The method provides a strong theoretical support for further manual investigation work

Heterostructured ZnFe2O4/Fe2TiO5/TiO2 Composite Nanotube Arrays with an Improved Photocatalysis Degradation Efficiency Under Simulated Sunlight Irradiation

Abstract To improve the visible light absorption and photocatalytic activity of titanium dioxide nanotube arrays (TONTAs), ZnFe2O4 (ZFO) nanocrystals were perfused into pristine TONTA pipelines using a novel bias voltage-assisted perfusion method. ZFO nanocrystals were well anchored on the inner walls of the pristine TONTAs when the ZFO suspensions (0.025 mg mL−1) were kept under a 60 V bias voltage for 1 h. After annealing at 750 °C for 2 h, the heterostructured ZFO/Fe2TiO5 (FTO)/TiO2 composite nanotube arrays were successfully obtained. Furthermore, Fe3+ was reduced to Fe2+ when solid solution reactions occurred at the interface of ZFO and the pristine TONTAs. Introducing ZFO significantly enhanced the visible light absorption of the ZFO/FTO/TONTAs relative to that of the annealed TONTAs. The coexistence of type I and staggered type II band alignment in the ZFO/FTO/TONTAs facilitated the separation of photogenerated electrons and holes, thereby improving the efficiency of the ZFO/FTO/TONTAs for photocatalytic degradation of methylene blue when irradiated with simulated sunlight

Heterogeneous fluid flow in fractured layered carbonates and its implication for generation of incipient karst

We use numerical models to investigate fluid flow in layered fractured carbonate rocks, and specifically to investigate the effects of the structural and hydraulic properties of both joints and bedding planes on flow localization. Synthetic fracture networks made up of two jointed layers separated by a horizontal bedding plane are generated to represent the typical layered fracture systems often formed in carbonate rocks. A uniform aperture field is assumed for each joint set and for the bedding plane, but different joint sets and the bedding plane can have non-identical values. The aperture ratio of the joint sets to the bedding plane is found to dominate the behaviour of flow heterogeneity on the bedding plane. Three distinct flow regimes, i.e. joint-dominated, transitional and bedding plane-dominated, are recognized. The magnitude of the aperture ratio controls which flow regime develops. We further suggest that the different flow regimes may be responsible for the initiation of different types of incipient karst morphologies observed in nature: pipe karst, stripe karst and sheet karst