11 research outputs found
Characterizing Håkon Mosby Mud Volcano (Barents Sea) cold seep systems by combining ROV-based acoustic data and underwater photogrammetry
Cold-seeps have a unique geo-ecological significance in the deep-sea environment. They impact the variability of present-day submarine sedimentary environments, affecting the evolution of the landscape over time and creating a variety of submarine landforms, one of which is Mud Volcanoes (MVs). MVs form due to the extrusion of mud, fluids, and gas, mainly methane, from deeper sedimentary layers. These natural gas seepage systems could significantly affect climate change and the global carbon cycle. We present a comprehensive method that combines ROV-based multibeam mapping and underwater photogrammetry to enhance the understanding of the physical relationships between geomorphic units characterizing the Håkon Mosby Mud Volcano (HMMV) and the distribution of associated habitats. HMMV is indeed characterized by high thermal and geochemical gradients from its center to the margins resulting in a clear zonation of chemosynthetic communities. Our approach integrates multi-resolutions and multi-sources data acquired using a work-class ROV. The ROV-based microbathymetry data helped to identify the different types of fine-scale submarine landforms in the central part of HMMV. This revealed three distinct geomorphic units, with the central hummocky region being the most complex. To further study this area, ROV images were analyzed using a defined Structure from Motion workflow producing millimetric resolution 2D and 3D models. Object-Based Image Analysis (OBIA), applied on orthomosaics, allowed us to obtain a fine classification of main benthic communities covering a total area of 940m2, including the active seepage area of the hummocky rim. Four major substrate types were identified in these regions: uncovered mud, bacterial mats high-density, bacterial mats low-density, sediments and tubeworms. Their relationship with terrain morphology and seepage activity were investigated at different spatial scales, contributing to a deeper understanding the ecological functioning of cold seep ecosystems in MVs. The applied workflow is proposed as an innovative processing technique for future studies on cold-seep systems. Geomorphic and ecological processes in extreme environments are inherently linked and marked by spatial patterns typifying associated habitats and sedimentary environments. This is poorly investigated in previous studies, leaving a substantial gap in the geomorphological drivers responsible for habitat distribution and extent in cold seep systems
Mesenchymal Stromal Cells (MSCs): An Ally of B-Cell Acute Lymphoblastic Leukemia (B-ALL) Cells in Disease Maintenance and Progression within the Bone Marrow Hematopoietic Niche
Mesenchymal stromal cells (MSCs) are structural components of the bone marrow (BM) niche, where they functionally interact with hematopoietic stem cells and more differentiated progenitors, contributing to hematopoiesis regulation. A growing body of evidence is nowadays pointing to a further crucial contribution of MSCs to malignant hematopoiesis. In the context of B-cell acute lymphoblastic leukemia (B-ALL), MSCs can play a pivotal role in the definition of a leukemia-supportive microenvironment, impacting on disease pathogenesis at different steps including onset, maintenance and progression. B-ALL cells hijack the BM microenvironment, including MSCs residing in the BM niche, which in turn shelter leukemic cells and protect them from chemotherapeutic agents through different mechanisms. Evidence is now arising that altered MSCs can become precious allies to leukemic cells by providing nutrients, cytokines, pro-survivals signals and exchanging organelles, as hereafter reviewed. The study of the mechanisms exploited by MSCs to nurture and protect B-ALL blasts can be instrumental in finding new druggable candidates to target the leukemic BM microenvironment. Some of these microenvironment-targeting strategies are already in preclinical or clinical experimentation, and if coupled with leukemia-directed therapies, could represent a valuable option to improve the prognosis of relapsed/refractory patients, whose management represents an unmet medical need
Characterizing Håkon Mosby Mud Volcano (Barents Sea) cold seep systems by combining ROV-based acoustic data and underwater photogrammetry
Cold-seep systems have a unique geo-ecological significance in the deep-sea environment. They impact the variability of present-day submarine sedimentary environments, affecting the evolution of the landscape over time and creating a variety of submarine landforms, one of which is Mud Volcanoes (MVs). MVs are submarine landforms form due extrusion of mud, fluids, and gas, mainly methane, from deeper sedimentary layers. These natural gas seepage systems could significantly affect climate change and the global carbon cycle.We present a comprehensive method that combines ROV-based multibeam mapping and underwater photogrammetry to enhance the understanding of the geomorphic units characterizing the Håkon Mosby Mud Volcano (HMMV) and the distribution of associated habitats. HMMV is indeed characterized by high thermal and geochemical gradients from its center to the margins resulting in a clear zonation of chemosynthetic communities. Our approach integrates multi-resolutions and multisources data acquired using a work-class ROV. The ROV-based microbathymetry data helped to identify the different types of fine-scale submarine landforms in the central part of HMMV. This revealed three distinct geomorphic units, with the central hummocky region being the most complex.To further study this area, ROV images were analyzed using a defined Structure from Motion workflow producing millimetric resolution 2D and 3D models.Object-Based Image Analysis (OBIA), applied on orthomosaics, allowed us to obtain a fine classification of main benthic communities covering a total area of 940m 2 , including the active seepage area of the hummocky rim. Four major substrate types were distinctly imaged in these regions: uncovered mud, bacterial mats high-density, bacterial mats low-density, sediments and tubeworms. Their relationship with terrain morphology and seepage activity was investigated at different scale, contributing to a deeper understanding the ecological functioning of cold seep ecosystems in MVs. The applied workflow is proposed as an innovative processing technique for future studies on cold-seep systems. Geomorphic, biogeochemical, and ecological processes in extreme environments are inherently linked and marked by spatial patterns typifying associated habitats and sedimentary environments. This is poorly investigated in previous studies, leaving a substantial gap in the geomorphological drivers responsible for habitat distribution and extent in cold seep systems
Functional Consequences of Low Activity of Transport System A for Neutral Amino Acids in Human Bone Marrow Mesenchymal Stem Cells
In cultured human fibroblasts, SNAT transporters (System A) account for the accumulation of non-essential neutral amino acids, are adaptively up-regulated upon amino acid deprivation and play a major role in cell volume recovery upon hypertonic stress. No information is instead available on the expression and activity of SNAT transporters in human bone marrow mesenchymal stromal cells (MSC), although they are increasingly investigated for their staminal and immunomodulatory properties and used for several therapeutic applications. The uptake of glutamine and proline, two substrates of SNAT1 and SNAT2 transporters, was measured in primary human MSC and an MSC line. The amino acid analogue MeAIB, a specific substrate of these carriers, has been used to selectively inhibit SNAT-dependent transport of glutamine and, through its sodium-dependent transport, as an indicator of SNAT1/2 activity. SNAT1/2 expression and localization were assessed with RT-PCR and confocal microscopy, respectively. Cell volume was assessed from urea distribution space. In all these experiments, primary human fibroblasts were used as the positive control for SNAT expression and activity. Compared with fibroblasts, MSC have a lower SNAT1 expression and hardly detectable membrane localization of both SNAT1 and SNAT2. Moreover, they exhibit no sodium-dependent MeAIB uptake or MeAIB-inhibitable glutamine transport, and exhibit a lower ability to accumulate glutamine and proline than fibroblasts. MSC exhibited an only marginal increase in MeAIB transport upon amino acid starvation and did not recover cell volume after hypertonic stress. In conclusion, the activity of SNAT transporters is low in human MSC. MSC adaptation to amino acid shortage is expected to rely on intracellular synthesis, given the absence of an effective up-regulation of the SNAT transporters
Geomorphology of coralligenous reefs offshore southeastern Sicily (Ionian Sea)
ABSTRACTCoralligenous (C) includes calcareous build-ups of biogenic origin, formed since the Holocene transgression. Peculiar columnar-shaped C outcrops were documented offshore Marzamemi village (SE Sicily, Ionian Sea), although the actual extension and distribution were not assessed. Project ‘CRESCIBLUREEF’ produced a new, 17 km2 high-resolution bathymetric map, leading to good knowledge about their extent in this area. C bioconstructions are largely distributed along two depth ranges 36–42 m and 86–102 m water depth. By coupling the documented uplift rate in this region and the Holocene sea-level curve, we were able to interpret the distribution of C outcrops over terraces. However, additional investigation is required to understand: (1) the role of the inherited continental shelf landscape, in creating a favorable substrate for the settlement and growth of C habitats during the Holocene, and (2) the extent to which C bioconstructions can impact the evolution of present-day continental shelf landforms and landscapes
An Arctic natural oil seep investigated from space to the seafloor
Due to climate change, decreasing ice cover and increasing industrial activities, Arctic marine ecosystems are expected to face higher levels of anthropogenic stress. To sustain healthy and productive ocean ecosystems, it is imperative to build baseline data to assess future changes. Herein, a natural oil seep site offshore western Svalbard (Prins Karls Forland, PKF, 80–100 m water depth), discovered using satellite radar images, was investigated using an extensive multiscale and multisource geospatial dataset collected by satellite, aerial, floating, and underwater platforms. The PKF seep covers roughly a seafloor area of 30,000 m2 and discharges oil from Tertiary or younger source rocks. Biomarker analyses confirm that the oil in the slicks on the sea surface and from the seep on the seafloor have the same origin. Uranium/Thorium dating of authigenic carbonate crusts indicated that the seep had emanated since the Late Pleistocene when ice sheet melting unlocked the hydrocarbons trapped beneath the ice. The faunal communities at the PKF seep are a mix of typical high latitude fauna and taxa adapted to reducing environments. Remarkably, the inhospitable oil-impregnated sediments were also colonized by abundant infaunal organisms. Altogether, in situ observations obtained at the site provide essential insights into the characteristics of high–latitude oil seeps and can be used as a natural laboratory for understanding the potential impacts of human oil discharge into the ocean
The Erasmus+ BridgET project: A European partnership to renew teaching in marine geosciences
editorial reviewedRecent advances in underwater and airborne robotic systems and ocean technologies have opened new perspectives in marine geology and its applications in the context of coastal and marine economic activities, whose sustainable development is increasingly acknowledged as a pillar for the new blue economy. BridgET (Bridging the gap between the land and the sea in a virtual Environment for innovative Teaching and community involvement in the science of climate change-induced marine and coastal geohazard) is an EU ERASMUS+ project designed to develop innovative and inclusive teaching methods to address a growing demand for strategic skills and scientific expertise in the field of 3D geological mapping of coastal environments. Seamless integration of the wide variety of multisource and multiscale onshore, nearshore and offshore geospatial data is indeed one of the main areas for improvement in the implementation of efficient management practices in coastal regions, where climate change, rising sea level, and geohazards are considerable environmental issues.BridgET involves a partnership consisting of six European universities with outstanding expertise in the study of geological hazards, and climate impacts in marine and coastal areas (i.e., University of Milano-Bicocca, Italy, Arctic University of Tromsø/CAGE - Norway, National and Kapodistrian University of Athens - Greece, Kiel University, Germany, University of Liege – Belgium, and the University of Malta), two Italian research institutes (INGV and INAF) and a German company (Orthodrone GmvH) specialized in UAS-based LiDAR and photogrammetry data acquisition services and analyses. Project implementation relies on delivering learning and teaching activities through dedicated summer schools for MSc students by efficiently combining the partner’s expertise. Schools focus on giving students a hands-on experience with the variety of methods and procedures adopted in geospatial data acquisition and processing, including the use of drones (Uncrewed Aerial System – UAS), acoustic remote sensing techniques and underwater robotic systems, together with      the progress made by computer visions and digital image analysis by using Artificial Intelligence (AI). Students are also introduced to the opportunity to easily examine multiple viewing angles of the seabed and coastal 3D surfaces by using immersive and non-immersive Virtual Reality (VR), to bring them closer to a more straightforward observation of geomorphological data and geological phenomena.The first Summer School was held in Santorini between the 3rd and 14th of October, 2022. It was attended by 26 students coming from 13 different countries. Teaching and learning activities included several classrooms, fieldwork, laboratory sessions, and seven seminars and cultural visits dealing with transversal topics, allowing students to approach an integrated understanding of human interaction with physical processes from social and economic perspectives. In this presentation, we give examples of course content used to allow students to develop a deeper understanding of theoretical and practical knowledge of climate-induced coastal and marine geohazards. Participants' opinions on the quality of the offered learning/training activities of the Erasmus+ BridgET Santorini Summer School (collected through a dedicated questionnaire) will also be presented
Monocyte–macrophage polarization and recruitment pathways in the tumour microenvironment of B‐cell acute lymphoblastic leukaemia
Summary
B‐cell acute lymphoblastic leukaemia (B‐ALL) reprograms the surrounding bone marrow (BM) stroma to create a leukaemia‐supportive niche. To elucidate the contribution of immune cells to the leukaemic microenvironment, we investigated the involvement of monocyte/macrophage compartments, as well as several recruitment pathways in B‐ALL development. Immunohistochemistry analyses showed that CD68‐expressing macrophages were increased in leukaemic BM biopsies, compared to controls and predominantly expressed the M2‐like markers CD163 and CD206. Furthermore, the "non‐classical" CD14
CD16
monocyte subset, expressing high CX3CR1 levels, was significantly increased in B‐ALL patients' peripheral blood. CX3CL1 was shown to be significantly upregulated in leukaemic BM plasma, thus providing an altered migratory pathway possibly guiding NC monocyte recruitment into the BM. Additionally, the monocyte/macrophage chemoattractant chemokine ligand 2 (CCL2) strongly increased in leukaemic BM plasma, possibly because of the interaction of leukaemic cells with mesenchymal stromal cells and vascular cells and due to a stimulatory effect of leukaemia‐related inflammatory mediators. C5a, a macrophage chemoattractant and M2‐polarizing factor, further appeared to be upregulated in the leukaemic BM, possibly as an effect of PTX3 decrease, that could unleash complement cascade activation. Overall, deregulated monocyte/macrophage compartments are part of the extensive BM microenvironment remodelling at B‐ALL diagnosis and could represent valuable targets for novel treatments to be coupled with classical chemotherapy
How Academics and the Public Experienced Immersive Virtual Reality for Geo-Education
Immersive virtual reality can potentially open up interesting geological
sites to students, academics and others who may not have had the
opportunity to visit such sites previously. We study how users perceive
the usefulness of an immersive virtual reality approach applied to Earth
Sciences teaching and communication. During nine immersive virtual
reality-based events held in 2018 and 2019 in various locations (Vienna
in Austria, Milan and Catania in Italy, Santorini in Greece), a large
number of visitors had the opportunity to navigate, in immersive mode,
across geological landscapes reconstructed by cutting-edge, unmanned
aerial system-based photogrammetry techniques. The reconstructed virtual
geological environments are specifically chosen virtual geosites, from
Santorini (Greece), the North Volcanic Zone (Iceland), and Mt. Etna
(Italy). Following the user experiences, we collected 459
questionnaires, with a large spread in participant age and cultural
background. We find that the majority of respondents would be willing to
repeat the immersive virtual reality experience, and importantly, most
of the students and Earth Science academics who took part in the
navigation confirmed the usefulness of this approach for geo-education
purposes
How Academics and the Public Experienced Immersive Virtual Reality for Geo-Education
Immersive virtual reality can potentially open up interesting geological sites to students,
academics and others who may not have had the opportunity to visit such sites previously. We study
how users perceive the usefulness of an immersive virtual reality approach applied to Earth Sciences
teaching and communication. During nine immersive virtual reality-based events held in 2018 and
2019 in various locations (Vienna in Austria, Milan and Catania in Italy, Santorini in Greece), a large
number of visitors had the opportunity to navigate, in immersive mode, across geological landscapes
reconstructed by cutting-edge, unmanned aerial system-based photogrammetry techniques. The
reconstructed virtual geological environments are specifically chosen virtual geosites, from Santorini
(Greece), the North Volcanic Zone (Iceland), and Mt. Etna (Italy). Following the user experiences, we
collected 459 questionnaires, with a large spread in participant age and cultural background. We find
that the majority of respondents would be willing to repeat the immersive virtual reality experience,
and importantly, most of the students and Earth Science academics who took part in the navigation
confirmed the usefulness of this approach for geo-education purposes