Caracterización microbiológica de Salmonella en alimentos de venta callejera en un sector universitario de Bogotá, Colombia. Julio a octubre de 2010

Microbiological characterization of Salmonella in Street-vended foods an university sector in Bogotá, Colombia. julyoctober2010Introduction. Salmonella belongs to the Enterobacteriaceae family, and is one of the main microorganisms involved in foodbornedisease. It produces a clinical entity known as salmonelosis can manifest as two different pathological processes, typhoid feveror gastroenteritis. The objective of this research was to demonstrate the presence of Salmonella spp. in street-vended foodsin an area bounded by Universities in the city of Bogotá and its subsequent characterization by means of clinical microbiologytechniques. Materials and Methods. Observational cross sectional study. Was analyze 42 samples of street-vended foods obtainedthrough a non-probability sampling, the isolation of Salmonella spp. was performed following the method proposed by the Foodand Drug Administration, performing biochemical identification and serological tests for Salmonella spp. and antimicrobialsusceptibility testing. Results. Growth was detected in a total of 18 samples (42.9%), of which only 2 were positive for Salmonellaenterica with a 11.1%, 11 out of 18 samples were positive for other bacteria belonging to the family Enterobacteriaceae (61.1%)and 5 samples without profile identified (27.8%). The samples tested were susceptible to ciprofloxacin susceptibility, showingresistance to Trimethoprim-Sulfamethoxazole, Chloramphenicol and Ampicillin. Conclusions. This investigation established thepresence of Salmonella enterica and other Enterobacteriaceae in street-vended foods, showing a high risk to the health ofresidents and student population of a university sector in the city of Bogotá, Colombia. Key words: Salmonella. Foodborne Diseases. Serotyping.Introducción. Salmonella spp. perteneciente a la familia Enterobacteriaceae, es uno de los principales microorganismosimplicados en las enfermedades transmitidas por alimentos. La infección conocida como salmonelosis se puede manifestarcomo dos procesos patológicos diferentes, la fiebre tifoidea o la gastroenteritis. El objetivo de esta investigación fue demostrarla presencia de Salmonella spp. en alimentos de venta callejera en un sector universitario delimitado de la ciudad de Bogotáy su posterior caracterización por medio de técnicas de microbiología clínica. Materiales y Métodos. Estudio observacionaldescriptivo de corte transversal. Se recolectaron 42 muestras de alimentos en ventas callejeras a través de un muestreono probabilístico. Para el aislamiento y caracterización de Salmonella spp. se realizó el método propuesto por la Food andDrug Administration, empleando pruebas bioquímicas, de serotipificación para Salmonella spp. y realizando la prueba desusceptibilidad antimicrobiana. Resultados. Se detectó crecimiento microbiano en un total de 18 muestras (42,9%), de lascuales solo dos fueron positivas por serotipificación para Salmonella enterica con un 11,1%, 11 de estas 18 muestras fueronpositivas para otras bacterias pertenecientes a la familia enterobacteriaceae (61,1%) y cinco muestras no pudieron seridentificadas (27,8%). Las cepas sometidas a antibiograma fueron susceptibles a Ciprofloxacina, y resistentes al Trimetoprimsulfametoxazol,cloramfenicol y ampicilina. Conclusiones. Esta investigación permitió identificar la presencia de Salmonellaentérica y otras enterobacterias en alimentos de venta callejera, lo cual puede representar un alto riesgo para la salud de loshabitantes y la población estudiantil de un sector universitario en Bogotá, Colombia. Palabras clave: Salmonella. Enfermedades transmitidas por los alimentos. Serotipificación.   &nbsp

Caracterización de especies de rickettsia en garrapatas (ixodidae) del municipio de Villeta, Cundinamarca y su relación con la epidemiología de la rickettsiosis en el mismo municipio

En Colombia, el municipio de Villeta (Cundinamarca) es considerado como una zona endémica para rickettsiosis del grupo de las fiebres manchadas (GFM), dados los casos de mortalidad en humanos por Rickettsia rickettsii en el periodo 2003-2004, y las altas tasas de seropositividad frente al GFM tanto en habitantes de zona rural, como en animales domésticos. A pesar de esto, no se conoce la especie o especies de Rickettsia que circulan en las garrapatas de este municipio, ni su posible relación con la epidemiología actual de la enfermedad en esta región de Cundinamarca. Es así como el objetivo de este estudio fue caracterizar especies de Rickettsia en garrapatas (Ixodidae) procedentes del municipio de Villeta, con el fin de establecer una probable relación con la epidemiología de la rickettsiosis en el mismo municipio.In Colombia, Villeta Town (Cundinamarca) is considered endemic zone for Spotted Fever Group (SFG) rickettsioses given the mortality in human cases by Rickettsia rickettsii in 2003-2004, and the high rates of seropositivity against SFG in inhabitants of rural areas and domestic animals. In spite of this, the circulating Rickettsia species in ticks and its possible relationship with the recent disease epidemiology for this Cundinamarca region is not known. Therefore, the main purpose of this study was to characterize the Rickettsia species in ticks (Ixodidae) from Villeta town, with the aim of establishing a probable connection with the rickettsioses epidemiology of the same town

Caracterización microbiológica de Salmonella en alimentos de venta callejera en un sector universitario de Bogotá, Colombia. Julio a octubre de 2010

Microbiological characterization of Salmonella in Street-vended foods an university sector in Bogotá, Colombia. julyoctober2010Introduction. Salmonella belongs to the Enterobacteriaceae family, and is one of the main microorganisms involved in foodbornedisease. It produces a clinical entity known as salmonelosis can manifest as two different pathological processes, typhoid feveror gastroenteritis. The objective of this research was to demonstrate the presence of Salmonella spp. in street-vended foodsin an area bounded by Universities in the city of Bogotá and its subsequent characterization by means of clinical microbiologytechniques. Materials and Methods. Observational cross sectional study. Was analyze 42 samples of street-vended foods obtainedthrough a non-probability sampling, the isolation of Salmonella spp. was performed following the method proposed by the Foodand Drug Administration, performing biochemical identification and serological tests for Salmonella spp. and antimicrobialsusceptibility testing. Results. Growth was detected in a total of 18 samples (42.9%), of which only 2 were positive for Salmonellaenterica with a 11.1%, 11 out of 18 samples were positive for other bacteria belonging to the family Enterobacteriaceae (61.1%)and 5 samples without profile identified (27.8%). The samples tested were susceptible to ciprofloxacin susceptibility, showingresistance to Trimethoprim-Sulfamethoxazole, Chloramphenicol and Ampicillin. Conclusions. This investigation established thepresence of Salmonella enterica and other Enterobacteriaceae in street-vended foods, showing a high risk to the health ofresidents and student population of a university sector in the city of Bogotá, ColombiaIntroducción. Salmonella spp. perteneciente a la familia Enterobacteriaceae, es uno de los principales microorganismosimplicados en las enfermedades transmitidas por alimentos. La infección conocida como salmonelosis se puede manifestarcomo dos procesos patológicos diferentes, la fiebre tifoidea o la gastroenteritis. El objetivo de esta investigación fue demostrarla presencia de Salmonella spp. en alimentos de venta callejera en un sector universitario delimitado de la ciudad de Bogotáy su posterior caracterización por medio de técnicas de microbiología clínica. Materiales y Métodos. Estudio observacionaldescriptivo de corte transversal. Se recolectaron 42 muestras de alimentos en ventas callejeras a través de un muestreono probabilístico. Para el aislamiento y caracterización de Salmonella spp. se realizó el método propuesto por la Food andDrug Administration, empleando pruebas bioquímicas, de serotipificación para Salmonella spp. y realizando la prueba desusceptibilidad antimicrobiana. Resultados. Se detectó crecimiento microbiano en un total de 18 muestras (42,9%), de lascuales solo dos fueron positivas por serotipificación para Salmonella enterica con un 11,1%, 11 de estas 18 muestras fueronpositivas para otras bacterias pertenecientes a la familia enterobacteriaceae (61,1%) y cinco muestras no pudieron seridentificadas (27,8%). Las cepas sometidas a antibiograma fueron susceptibles a Ciprofloxacina, y resistentes al Trimetoprimsulfametoxazol,cloramfenicol y ampicilina. Conclusiones. Esta investigación permitió identificar la presencia de Salmonellaentérica y otras enterobacterias en alimentos de venta callejera, lo cual puede representar un alto riesgo para la salud de loshabitantes y la población estudiantil de un sector universitario en Bogotá, Colombia

First molecular detection of Rickettsia sp. strain Atlantic rainforest in Amblyomma ovale ticks from Espírito Santo state, Brazil

Abstract Espírito Santo state (southeastern Brazil) is considered an endemic area for spotted fever group rickettsioses. In February 2017, we received in our laboratory seven unfed Amblyomma ovale adult ticks collected by a farmer from his clothes and body (not attached) during a working day in the rural area of Ibiraçu municipality, Espírito Santo state. By polymerase chain reaction (PCR) analyses, targeting gltA and ompA rickettsial genes, the DNA of Rickettsia was detected in 6/7 (85.7%) A. ovale. In all cases, DNA sequencing of PCR products revealed that consensus sequences of both genes were 100% identical to gltA and ompA corresponding sequences of Rickettsia sp. strain Atlantic rainforest retrieved from GenBank. This study reports the first molecular detection of Rickettsia sp. strain Atlantic rainforest in A. ovale ticks from Espírito Santo state. Our findings indicate a new Brazilian state in the southeast region at risk of human infection with this tick-borne emerging rickettsial agent

Epididymo-orchitis caused by Histoplasma capsulatumin a Colombian patient

Abstract Although histoplasmosis is generally a self-limited disease, disseminated infection can occur in patients lacking effective cell-mediated immunity, reaching virtually every organ, even the genitourinary tract in rare cases. We report a case of epididymo-orchitis in an immunocompetent 38-year-old bricklayer from the rural area of Villeta, Cundinamarca, Colombia. The patient presented with testicular pain and macroscopic scrotal changes requiring a left orchiectomy, with microbiological isolation and molecular confirmation of Histoplasma capsulatum

First molecular detection of Rickettsia sp. strain Atlantic rainforest in Amblyomma ovale ticks from Espírito Santo state, Brazil

<div><p>Abstract Espírito Santo state (southeastern Brazil) is considered an endemic area for spotted fever group rickettsioses. In February 2017, we received in our laboratory seven unfed Amblyomma ovale adult ticks collected by a farmer from his clothes and body (not attached) during a working day in the rural area of Ibiraçu municipality, Espírito Santo state. By polymerase chain reaction (PCR) analyses, targeting gltA and ompA rickettsial genes, the DNA of Rickettsia was detected in 6/7 (85.7%) A. ovale. In all cases, DNA sequencing of PCR products revealed that consensus sequences of both genes were 100% identical to gltA and ompA corresponding sequences of Rickettsia sp. strain Atlantic rainforest retrieved from GenBank. This study reports the first molecular detection of Rickettsia sp. strain Atlantic rainforest in A. ovale ticks from Espírito Santo state. Our findings indicate a new Brazilian state in the southeast region at risk of human infection with this tick-borne emerging rickettsial agent.</p></div