Can morphological features of coccolithophores serve as a reliable proxy to reconstruct environmental conditions of the past?

Morphological changes in coccoliths, tiny calcite platelets covering the outer surface of coccolithophores, can be induced by physiological responses to environmental changes. Coccoliths recovered from sedimentary successions may therefore provide information on paleo-environmental conditions prevailing at the time when the coccolithophores were alive. To calibrate the biomineralization responses of ancient coccolithophore to environmental changes, studies often compared the biological responses of living coccolithophore species with paleo-data from calcareous nannofossils. However, there is uncertainty whether the morphological responses of living coccolithophores are representative of those of the fossilized ancestors. To investigate this, we exposed four living coccolithophore species (Emiliania huxleyi, Gephyrocapsa oceanica, Coccolithus pelagicus subsp. braarudii, and Pleurochrysis carterae) that have been evolutionarily distinct for hundreds of thousands to millions of years, to a range of environmental conditions (i.e., changing light intensity, Mg∕Ca ratio, nutrient availability, temperature, and carbonate chemistry) and evaluated their responses in coccolith morphology (i.e., size, length, width, malformation). The motivation for this study was to test if there is a consistent morphological response of the four species to changes in any of the tested abiotic environmental factors. If this was the case, then this could suggest that coccolith morphology can serve as a paleo-proxy for that specific factor because this response is conserved across species that have been evolutionary distinct over geological timescales. However, we found that the four species responded differently to changing light intensity, Mg∕Ca ratio, nutrient availability, and temperature in terms of coccolith morphology. The lack of a common response reveals the difficulties in using coccolith morphology as a paleo-proxy for these environmental drivers. However, a common response was observed under changing seawater carbonate chemistry (i.e., rising CO2), which consistently induced malformations. This commonality provides some confidence that malformations found in the sedimentary record could be indicative of adverse carbonate chemistry conditions

CALCAREOUS NANNOPLANKTON RESPONSE TO THE LATEST CENOMANIAN OCEANIC ANOXIC EVENT 2 PERTURBATION

Morphometric analyses were performed on Biscutum constans, Zeugrhabdotus erectus, Discorhabdus rotatorius and Watznaueria barnesiae specimens from five sections spanning the Cenomanian-Turonian boundary interval including Oceanic Anoxic Event (OAE) 2 (~ 94 Ma). The study provides evidence for size fluctuations and dwarfism of B. constans during OAE 2, followed by a partial recovery at the end of the event: this taxon appears to be the most sensitive species, with similar and coeval size trends in all the analyzed sections. Conversely, morphometry shows negligible or unsystematic coccolith variations in Z. erectus, D. rotatorius and W. barnesiae. The comparison of OAE 2 data with those available for the early Aptian OAE 1a and latest Albian OAE 1d, indicates that B. constans repeatedly underwent size reduction and temporary dwarfism possibly implying that the same paleoenvironmental factors controlled calcification of B. constans during subsequent OAEs although the amplitude of B. constans coccolith reduction is significantly larger for OAE 1a than OAE 2. Paleoceanographic reconstructions suggest that ocean chemistry related to the amount of CO2 and toxic metal concentrations played a central role in B. constans coccolith secretion, while temperature and nutrient availability do not seem to have been crucial. Contrary to OAE 1a, Z. erectus, D. rotatorius and W. barnesiae appear to be substantially unrelated to OAE 2 paleoenvironmental stress, possibly because of different degrees of perturbation.  &nbsp

Impact of trace metal concentrations on coccolithophore growth and morphology: laboratory simulations of Cretaceous stress

The Cretaceous ocean witnessed intervals of profound perturbations such as volcanic input of large amounts of CO2, anoxia, eutrophication and introduction of biologically relevant metals. Some of these extreme events were characterized by size reduction and/or morphological changes of a few calcareous nannofossil species. The correspondence between intervals of high trace metal concentrations and coccolith dwarfism suggests a negative effect of these elements on nannoplankton biocalcification processes in past oceans. In order to test this hypothesis, we explored the potential effect of a mixture of trace metals on growth and morphology of four living coccolithophore species, namely Emiliania huxleyi, Gephyrocapsa oceanica, Pleurochrysis carterae and Coccolithus pelagicus. The phylogenetic history of coccolithophores shows that the selected living species are linked to Mesozoic species showing dwarfism under excess metal concentrations. The trace metals tested were chosen to simulate the environmental stress identified in the geological record and upon known trace metal interactions with living coccolithophore algae. Our laboratory experiments demonstrated that elevated trace metal concentrations, similarly to the fossil record, affect coccolithophore algae size and/or weight. Smaller coccoliths were detected in E. huxleyi and C. pelagicus, while coccoliths of G. oceanica showed a decrease in size only at the highest trace metal concentrations. P. carterae coccolith size was unresponsive to changing trace metal concentrations. These differences among species allow discriminating the most- (P. carterae), intermediate- (E. huxleyi and G. oceanica) and least-tolerant (C. pelagicus) taxa. The fossil record and the experimental results converge on a selective response of coccolithophores to metal availability. These species-specific differences must be considered before morphological features of coccoliths are used to reconstruct paleo-chemical conditions

Phytoplankton Response to Increased Nickel in the Context of Ocean Alkalinity Enhancement

Ocean alkalinity enhancement (OAE) is considered one of the most promising approaches to actively remove carbon dioxide (CO2) from the atmosphere by accelerating the natural process of rock weathering. This approach involves introducing alkaline substances sourced from natural mineral deposits such as olivine, basalt, and carbonates or obtained from industrial waste products such as steel slags, into seawater and dispersing them over coastal areas. Some of these natural and industrial substances contain trace metals, which would be released into the oceans along with the alkalinity enhancement. The trace metals could serve as micronutrients for marine organisms at low concentrations, but could potentially become toxic at high concentrations, adversely affecting marine biota. To comprehensively assess the feasibility of OAE, it is crucial to understand how the phytoplankton, which forms the base of marine food webs, responds to ocean alkalinization and associated trace metal perturbations. In this study, we investigated the toxicity of nickel on three representative phytoplankton species across a range of Ni concentrations (from 0 to 100 µmol L-1 with 12 µmol L-1 synthetic organic ligand). The results showed that the growth of the tested species was impacted differently. The low growth inhibition and high IC50 (concentration to inhibit growth rate by 50 %) revealed that both the coccolithophore Emiliania huxleyi and the dinoflagellate Amphidinium carterae were mildly impacted by the increase in Ni concentrations while the rapid response to exposure of Ni, high growth rate inhibition, and low IC50 of Thalassiosira weissflogii indicate low tolerance to Ni in this species. In conclusion, the variability in phytoplankton sensitivity to Ni suggests that for OAE applications with Ni-rich materials caution is required and critical toxic thresholds for Ni must be avoided

CALCAREOUS NANNOFOSSIL TAXONOMY AND BIOSTRATIGRAPHY OF THE TOARCIAN-LOWER BAJOCIAN COLLE DI SOGNO SECTION (LOMBARDY BASIN, SOUTHERN ALPS, ITALY)

Calcareous nannofossil biostratigraphy was conducted in the Toarcian-lower Bajocian interval at Colle di Sogno (Lombardy Basin, Southern Alps, Northern Italy) where the type-section of the Sogno Formation consisting of pelagic marly limestone, marlstone and marly claystone was established. Semiquantitative analyses of calcareous nannofossil assemblages allowed to achieve a high-resolution biostratigraphy based on several biohorizons, including zonal/subzonal markers and additional events. The NJT5 to NJT9 Zones of the standard nannofossil zonation established for the Mediterranean Province were identified. The biostratigraphy obtained at Colle di Sogno was compared to published nannofossil events calibrated with ammonite zones in sections from SE Spain, S France, Portugal and N Algeria. This assessment evidenced some discrepancies in the succession of events of the standard zonation and, furthermore, resulted in the revision of the age of a few datums. Moreover, some new/additional nannofossil biohorizons are proposed as subzonal markers. A morphometric analysis of the genus Watznaueria was conducted to identify diagnostic features for unambiguous species identification. The species W. colacicchii and W. contracta are distinguished on the basis of the coccolith width/central area width ratio, whereas W. britannica britannica is discriminated by the size - as in the original definition of the species - relative to the new subspecies W. britannica minor. The new species W. gaetanii differs from all other Watznaueria taxa by the bridge ultrastructure. In the Toarcian-Aalenian interval a progressive increase in size of Watznaueria specimens is paralleled by the progressive closure of the central area and the modification of the central area structure passing from a cross (W. colacicchii and W. contracta) to a double-button bridge (W. gaetanii) to a single-button bridge (W. britannica). These intrageneric evolutionary innovations accelerated in the Aalenian under stable paleoceanographic conditions and an oligotrophic regime

Taxonomic revision of the genus Carinolithus (Early - Middle Jurassic) based on morphometric analyses and diagenesis observations: Implications for biostratigraphy and evolutionary trends

International audienc

Growth response of Emiliania huxleyi to ocean alkalinity enhancement

The urgent necessity of reducing greenhouse gas emissions is coupled with a pressing need for widespread implementation of carbon dioxide removal (CDR) techniques to limit the increase in mean global temperature to levels below 2 °C compared to pre-industrial times. One proposed CDR method, Ocean Alkalinity Enhancement (OAE), mimics natural rock weathering processes by introducing suitable minerals into the ocean thereby increasing ocean alkalinity and promoting CO2 chemical absorption. While theoretical studies hold promise for OAE as a climate mitigation strategy, careful consideration of its ecological implications is essential. Indeed, the ecological impacts of enhanced alkalinity on marine organisms remain a subject of investigation as they may lead to changes in species composition. OAE implicates favourable conditions for calcifying organisms by enhancing the saturation state of calcium carbonate and decreasing the energetic costs for calcification. This may affect marine primary production by improving conditions for calcifying phytoplankton, among which coccolithophores play the leading role. They contribute <10 % to the global marine primary production, but are responsible for a large proportion of the marine calcite deposition. While previous research has extensively studied the effects of ocean acidification on coccolithophores, fewer studies have explored the impacts of elevated pH and alkalinity. In this context, we studied the sensitivity of Emiliania huxleyi, the most widespread coccolithophore species, to ocean alkalinity enhancement in a culture experiment. We monitored the species’ growth and calcification response to progressively increasing levels of total alkalinity (TA). Above a change in total alkalinity (ΔTA) of ~ 600 µmol kg-1, as CO2 concentrations decreased, E. huxleyi growth rate diminished, suggesting a threshold CO2 concentration of ~ 100 μatm necessary for optimal growth. The cellular calcite to organic carbon ratio (PIC:POC) remained stable over the total alkalinity range. Due to the decreasing growth rate in response to alkalinity enhancement, total carbonate formation was lower. OAE is rapidly advancing and has already reached the field-testing stage. Hence, our study contributes to the most critical part of investigations required to comprehend potential biological implications before large-scale OAE will be adapted

Neutron thermalization in plane lattices

info:eu-repo/semantics/publishe

Carbon-and oxygen-isotope signature of the Toarcian Oceanic Anoxic Event:insights from two Tethyan pelagic sequences (Gajum and Sogno Cores – Lombardy Basin, northern Italy)

The early Toarcian Oceanic Anoxic Event (T-OAE) was associated with major climatic changes involving profound effects on the global carbon cycle. In this study, we present new carbon-and oxygen-isotope, CaCO3 and total organic carbon (TOC) records from two cores (Sogno and Gajum Cores) that recovered pelagic successions from north-western Tethys. A palaeobathymetry of about 1000 and 1500 m water depth is tentatively reconstructed for the Gajum and Sogno sites, respectively. The investigated sections thereby represent some of the deepest records of the T-OAE in the western Tethys. During the early Toarcian, sedimentation in the Lombardy Basin (Southern Alps, northern Italy) was characterized by the deposition of the Fish Level (Livello a Pesci), a dark grey to black marly claystone with low CaCO3 content and relatively high TOC content. In the two cores, the Fish Level (similar to 5 m and similar to 15 m-thick at Sogno and Gajum, respectively) is subdivided into three lithostratigraphic intervals: a lower part, with minimum CaCO3 (5-10 %) and TOC (similar to 0.2-0.3 %) values; a central part with a progressive increase in TOC up to similar to 1.4 %, and an upper part characterized by the highest TOC up to similar to 2.5 %. Within the Fish Level a lower grey interval and an upper black interval are defined based on lithological features. Carbon-isotope chemostratigraphy resolves a delta C-13(carb) negative excursion of similar to 3 parts per thousand at Sogno and similar to 6 parts per thousand at Gajum, and a delta C-13(org) negative excursion of similar to 7 parts per thousand at both locations. This global carbon cycle anomaly, named the 'Jenkyns Event', is here subdivided into a lower J1 and an upper J2 segment. As highlighted by lithostratigraphic evidence, nannofossil biostratigraphy and chemostratigraphic correlations, a hiatus elides part of the succession below the Fish Level in the Gajum Core, although without compromising the completeness of the Fish Level itself. High-resolution delta C-13 data indicate that the base of the Fish Level is synchronous, but the top diachronous at the two coring sites. The same synchroneity of the base and diachroneity of the top of the black shale interval is identified in the Umbria-Marche Basin, suggesting that the duration of anoxia was not identical over very modest to relatively long distances

Assessing the impact of Ocean Alkalinity Enhancement on the zooplankton community

Ocean alkalinity enhancement (OAE) can help mitigate climate change impacts by increasing the carbon storage capacity of the ocean. The technique involves addition of alkaline substances to the seawater to accelerate the natural rock weathering process. However, this will lead to sudden seawater chemistry changes, such as increased pH that might directly and/or indirectly (through trophic pathways) affect zooplankton, an important trophic link, by altering its metabolic state and community composition. In addition, varying dilution times of alkaline substances might impact organisms differently. To date, the possible influences of OAE on zooplankton communities are largely unexplored. To bridge the knowledge gap, we conducted mesocosm and laboratory experiments in simulated non-equilibrated, calcium-based (Ca(OH)2) OAE setups. An incrementally enhanced alkalinity gradient from 0 to 1250 &amp;#181;mol kg-1 in steps of 250 &amp;#181;mol kg-1 was used in all experiments. The wide-ranging enhanced total alkalinity (&amp;#8710;TA) was selected to assess the safety threshold. In addition, we compared immediate versus delayed dilution scenarios in our mesocosm study, where each scenario ended up with the same &amp;#8710;TA gradient after mixing. We examined the multitrophic community response by monitoring twelve mesocosms for 39 days including the natural spring bloom community of Helgoland roads waters in the North Sea. Subsequently, the direct effect of alkalinity enhancement on the physiology (i.e., respiration and grazing) of Temora longicornis (predominant copepod in the mesocosms) was evaluated in the laboratory. The species-specific bottom-up effect was examined by culturing Rhodomonas salina in aforementioned &amp;#8710;TA gradient and feeding them to the T. longicornis. We observed relatively lower zooplankton abundance, and growth rate in mesocosms with &amp;#8710;TA1000 and 1250 &amp;#181;mol kg-1, which might be a bottom-up effect. In our lab experiments, though, we observed a negative impact on R. salina growth rate and nutritional quality from &amp;#8710;TA750 &amp;#181;mol kg-1, we did not detect any substantial direct or indirect impact on the physiological performance of T. longicornis. Overall, our laboratory study provided a preliminary understanding of the direct and indirect effects of OAE on a key copepod species, and the mesocosm study gave insight into the zooplankton community response.</jats:p