State of the Animal: monitoring animal welfare and health in The Netherlands (0-measurement) : summary, full report is available in Dutch (report 323)

25 measuring points summarise the results of policy measures for animal welfare and health in The Netherland

HERschel Observations of Edge-on Spirals (HEROES). II: Tilted-ring modelling of the atomic gas disks

Context. Edge-on galaxies can offer important insights in galaxy evolution as they are the only systems where the distribution of the different components can be studied both radially and vertically. The HEROES project was designed to investigate the interplay between the gas, dust, stars and dark matter (DM) in a sample of 7 massive edge-on spiral galaxies. Aims. In this second HEROES paper we present an analysis of the atomic gas content of 6 out of 7 galaxies in our sample. The remaining galaxy was recently analysed according to the same strategy. The primary aim of this work is to constrain the surface density distribution, the rotation curve and the geometry of the gas disks in a homogeneous way. In addition we identify peculiar features and signs of recent interactions. Methods. We construct detailed tilted-ring models of the atomic gas disks based on new GMRT 21-cm observations of NGC 973 and UGC 4277 and re-reduced archival HI data of NGC 5907, NGC 5529, IC 2531 and NGC 4217. Potential degeneracies between different models are resolved by requiring a good agreement with the data in various representations of the data cubes. Results. From our modelling we find that all but one galaxy are warped along the major axis. In addition, we identify warps along the line of sight in three galaxies. A flaring gas layer is required to reproduce the data only for one galaxy, but (moderate) flares cannot be ruled for the other galaxies either. A coplanar ring-like structure is detected outside the main disk of NGC 4217, which we suggest could be the remnant of a recent minor merger event. We also find evidence for a radial inflow of 15 +- 5 km/s in the disk of NGC 5529, which might be related to the ongoing interaction with two nearby companions. (Abridged)Comment: 39 pages, 38 figures, Accepted for publication in Astronomy & Astrophysic

Terpene synthases in cucumber (<i>Cucumis sativus</i>) and their contribution to herbivore-induced volatile terpenoid emission

Terpenoids play important roles in flavour, pollinator attraction and defence of plants. In cucumber (Cucumis sativus) they are important components of the herbivore‐induced plant volatile blend that attracts natural enemies of herbivores. We annotated the cucumber TERPENE SYNTHASE gene (CsTPS) family and characterized their involvement in the response towards herbivores with different feeding guilds using a combined molecular and biochemical approach. Transcripts of multiple CsTPS genes were upregulated in leaves upon herbivory and the products generated by the expressed proteins match the terpenoids recorded in the volatile blend released by herbivore‐damaged leaves. Spatial and temporal analysis of the promoter activity of CsTPS genes showed that cell content‐feeding spider mites (Tetranychus urticae) and thrips (Frankliniella occidentalis) induced promoter activity of CsTPS9 and CsTPS19 within hours after initiation of infestation, while phloem‐feeding aphids (Myzus persicae) induced CsTPS2 promoter activity. Our findings offer detailed insights into the involvement of the TPS gene family in the dynamics and fine‐tuning of the emission of herbivore‐induced plant volatiles in cucumber, and open a new avenue to understand molecular mechanisms that affect plant–herbivore interactions

Blocking T cell co-stimulation in primary Sjögren's syndrome:rationale, clinical efficacy and modulation of peripheral and salivary gland biomarkers

There is accumulating evidence that patients with primary Sjögren's syndrome (pSS) display aberrant CD4+ T cell responses, both in the peripheral compartment and in the inflamed salivary glands. CD4+ T cell abnormalities are also critically associated with B cell hyper activation, one of the hallmarks of disease, which is linked with disease severity and evolution to lymphoma. T cell activation and the cross-talk between T and B cells are tightly regulated by the balance between co-stimulatory pathways, such as the interactions between CD80/CD86:CD28, CD40:CD40L and ICOS:ICOSL, and co-inhibitory signals, including the immunoregulatory CTLA-4 protein. Evidence from patients with pSS as well as data from animal models of the disease suggests that these pathways play a critical role in pSS pathogenesis and their targeting could be exploited for therapeutic purposes. In this review, we first summarise the evidence implicating aberrant T cell co-stimulation and co-inhibition in driving the disease before focusing on the results of recent randomised controlled trials (RCTs) with compounds able to block T cell co-stimulation and enhance T cell co-inhibition. Despite a clear biological effect on downstream B cell activation has been observed in patients treated with CTLA-4-Ig (abatacept) and with monoclonal antibodies targeting CD40 and ICOSL, the clinical efficacy of this approach has so far yielded mixed results; while the anti-CD40 monoclonal antibody iscalimab showed significant improvement in systemic disease activity compared to placebo, two large RCTs with abatacept and a phase IIa RCT with an anti-ICOSL monoclonal antibody (prezalumab) failed to reach their primary endpoints. Although the discrepancies between biological and clinical efficacy of targeting T cell co-stimulation on pSS remain unresolved, several factors including drug bioavailability and receptor occupancy, patient stratification based on T-cell related biomarkers and the choice of study outcome are likely to play an important role and form the basis for further work towards the quest for a disease-modifying biologic therapy in pSS