A Type Inference Algorithm for Secure Ambients

We consider a type discipline for the Ambient Calculus that associates ambients with security levels and constrains them to be traversed by or opened in ambients of higher security clearance only. We present a bottom-up algorithm that, given an untyped process $P$, computes a minimal set of constraints on security levels such that all actions during runs of $P$ are performed without violating the security level priorities. Such an algorithm appears to be a prerequisite to use type systems to ensure security properties in the web scenario

Space-Aware Ambients and Processes

Resource control has attracted increasing interest in foundational research on distributed systems. This paper focuses on space control and develops an analysis of space usage in the context of an ambient-like calculus with bounded capacities and weighed processes, where migration and activation require space. A type system complements the dynamics of the calculus by providing static guarantees that the intended capacity bounds are preserved throughout the computation

MODULAZIONE DEL CFTR DA PARTE DELLA VARIANTE IPERTENSIVA DELLA PROTEINA CITOSCHELETRICA ADDUCINA

CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) is a chloride channel, expressed in the kidney, whose activity and localization are influenced by the cytoskeleton, in particular by actin and its polymerization state. In this study, a possible functional modification of CFTR activity and expression, led by the expression of the hypertensive mutations of adducin (G460W-S586C in humans), an actin capping protein, has been investigated. The experiments were performed on HEK293 T cells cotransfected with CFTR and the human wild-type or mutated adducin. In whole-cell patch-clamp experiments, both the CFTR chloride current and the slope of current activation after forskolin addition were significantly higher in HEK cells overexpressing mutated adducin. A higher plasma membrane density of active CFTR channels in the presence of mutated adducin, was confirmed by cell-attached patch-clamp experiments. Western blot and biotinylation experiments demonstrated an increased CFTR expression in the plasma membrane, in the presence of the mutated adducin. FRET (Fluorescence Resonance Energy Transfer) experiments showed a significant but weak interaction between CFTR and adducin, while coimmunoprecipitation experiments failed to reveal a clear interaction between the two proteins. A higher retention of CFTR in the plasma membrane was demonstrated both by FRAP (Fluorescence Recovery After Photobleaching) and photoactivation experiments. The present data indicate that, in HEK cells, the presence of the G460W-S586C hypertensive variant of adducin increases CFTR channel activity, possibly by altering its membrane turnover and inducing a retention of the channel in the plasma membrane. Since CFTR is known to modulate the activity of many others transport systems, the increased surface expression of the channel could have consequences on the whole network of transport in kidney cells

Cost benefit analysis of space communications technology. Volume 2: Final report

For abstract, see preceding accession

RPO, Second-order Contexts, and Lambda-calculus

First, we extend Leifer-Milner RPO theory, by giving general conditions to obtain IPO labelled transition systems (and bisimilarities) with a reduced set of transitions, and possibly finitely branching. Moreover, we study the weak variant of Leifer-Milner theory, by giving general conditions under which the weak bisimilarity is a congruence. Then, we apply such extended RPO technique to the lambda-calculus, endowed with lazy and call by value reduction strategies. We show that, contrary to process calculi, one can deal directly with the lambda-calculus syntax and apply Leifer-Milner technique to a category of contexts, provided that we work in the framework of weak bisimilarities. However, even in the case of the transition system with minimal contexts, the resulting bisimilarity is infinitely branching, due to the fact that, in standard context categories, parametric rules such as the beta-rule can be represented only by infinitely many ground rules. To overcome this problem, we introduce the general notion of second-order context category. We show that, by carrying out the RPO construction in this setting, the lazy observational equivalence can be captured as a weak bisimilarity equivalence on a finitely branching transition system. This result is achieved by considering an encoding of lambda-calculus in Combinatory Logic.Comment: 35 pages, published in Logical Methods in Computer Scienc

p75 neurotrophin receptor is a clock gene that regulates oscillatory components of circadian and metabolic networks.

The p75 neurotrophin receptor (p75(NTR)) is a member of the tumor necrosis factor receptor superfamily with a widespread pattern of expression in tissues such as the brain, liver, lung, and muscle. The mechanisms that regulate p75(NTR) transcription in the nervous system and its expression in other tissues remain largely unknown. Here we show that p75(NTR) is an oscillating gene regulated by the helix-loop-helix transcription factors CLOCK and BMAL1. The p75(NTR) promoter contains evolutionarily conserved noncanonical E-box enhancers. Deletion mutagenesis of the p75(NTR)-luciferase reporter identified the -1039 conserved E-box necessary for the regulation of p75(NTR) by CLOCK and BMAL1. Accordingly, gel-shift assays confirmed the binding of CLOCK and BMAL1 to the p75(NTR-)1039 E-box. Studies in mice revealed that p75(NTR) transcription oscillates during dark and light cycles not only in the suprachiasmatic nucleus (SCN), but also in peripheral tissues including the liver. Oscillation of p75(NTR) is disrupted in Clock-deficient and mutant mice, is E-box dependent, and is in phase with clock genes, such as Per1 and Per2. Intriguingly, p75(NTR) is required for circadian clock oscillation, since loss of p75(NTR) alters the circadian oscillation of clock genes in the SCN, liver, and fibroblasts. Consistent with this, Per2::Luc/p75(NTR-/-) liver explants showed reduced circadian oscillation amplitude compared with those of Per2::Luc/p75(NTR+/+). Moreover, deletion of p75(NTR) also alters the circadian oscillation of glucose and lipid homeostasis genes. Overall, our findings reveal that the transcriptional activation of p75(NTR) is under circadian regulation in the nervous system and peripheral tissues, and plays an important role in the maintenance of clock and metabolic gene oscillation

Cost benefit analysis of space communications technology: Volume 1: Executive summary

The questions of (1) whether or not NASA should support the further development of space communications technology, and, if so, (2) which technology's support should be given the highest priority are addressed. Insofar as the issues deal principally with resource allocation, an economics perspective is adopted. The resultant cost benefit methodology utilizes the net present value concept in three distinct analysis stages to evaluate and rank those technologies which pass a qualification test based upon probable (private sector) market failure. User-preference and technology state-of-the-art surveys were conducted (in 1975) to form a data base for the technology evaluation. The program encompassed near-future technologies in space communications earth stations and satellites, including the noncommunication subsystems of the satellite (station keeping, electrical power system, etc.). Results of the research program include confirmation of the applicability of the methodology as well as a list of space communications technologies ranked according to the estimated net present value of their support (development) by NASA

Defining the role of the Bcl-2 family proteins in Huntington's disease

B-cell lymphoma 2 (Bcl-2) family proteins regulate survival, mitochondria morphology dynamics and metabolism in many cell types including neurons. Huntington's disease (HD) is a neurodegenerative disorder caused by an expanded CAG repeat tract in the IT15 gene that encodes for the protein huntingtin (htt). In vitro and in vivo models of HD and HD patients' tissues show abnormal mitochondrial function and increased cell death rates associated with alterations in Bcl-2 family protein expression and localization. This review aims to draw together the information related to Bcl-2 family protein alterations in HD to decipher their potential role in mutated htt-related cell death and mitochondrial dysfunction

On the Construction of Sorted Reactive Systems

We develop a theory of sorted bigraphical reactive systems. Every application of bigraphs in the literature has required an extension, a sorting, of pure bigraphs. In turn, every such application has required a redevelopment of the theory of pure bigraphical reactive systems for the sorting at hand. Here we present a general construction of sortings. The constructed sortings always sustain the behavioural theory of pure bigraphs (in a precise sense), thus obviating the need to redevelop that theory for each new application. As an example, we recover Milner’s local bigraphs as a sorting on pure bigraphs. Technically, we give our construction for ordinary reactive systems, then lift it to bigraphical reactive systems. As such, we give also a construction of sortings for ordinary reactive systems. This construction is an improvement over previous attempts in that it produces smaller and much more natural sortings, as witnessed by our recovery of local bigraphs as a sorting

Deriving Bisimulation Congruences: 2-categories vs precategories

G-relative pushouts (GRPOs) have recently been proposed by the authors as a new foundation for Leifer and Milner’s approach to deriving labelled bisimulation congruences from reduction systems. This paper develops the theory of GRPOs further, arguing that they provide a simple and powerful basis towards a comprehensive solution. As an example, we construct GRPOs in a category of ‘bunches and wirings.’ We then examine the approach based on Milner’s precategories and Leifer’s functorial reactive systems, and show that it can be recast in a much simpler way into the 2-categorical theory of GRPOs