Calcium isotopic composition of high-latitude proxy carrier Neogloboquadrina pachyderma (sin.)

The accurate reconstruction of sea surface temperature (SST) history in climate-sensitive regions (e.g. tropical and polar oceans) became a challenging task in palaeoceanographic research. Biogenic shell carbonate SST proxies successfully developed for tropical regions often fail in cool water environments. Their major regional shortcomings and the cryptic diversity now found within the major high latitude proxy carrier Neogloboquadrina pachyderma (sin.) highlight an urgent need to explore complementary SST proxies for these cool-water regions. Here we incorporate the genetic component into a calibration study of a new SST proxy for the high latitudes. We found that the calcium isotopic composition (δ44/40Ca) of calcite from genotyped net catches and core-top samples of the planktonic foraminifera Neogloboquadrina pachyderma (sin.) is related to temperature and unaffected by genetic variations. The temperature sensitivity has been found to be 0.17 (±0.02)‰ per 1°C, highlighting its potential for downcore applications in open marine cool-water environments. Our results further indicate that in extreme polar environments, below a critical threshold temperature of 2.0 (±0.5)°C associated with salinities below 33.0 (±0.5)‰, a prominent shift in biomineralization affects the δ44/40Ca of genotyped and core-top N. pachyderma (sin.), becoming insensitive to temperature. These findings highlight the need of more systematic calibration studies on single planktonic foraminiferal species in order to unravel species-specific factors influencing the temperature sensitivity of Ca isotope fractionation and to validate the proxies' applicability

Section 301 of the Trade Act of 1974: Requirements, Procedures, and Developments

The recent dramatic increase in the use of Section 301 of the Trade Act of 1974 ( § 301 ) and a predecessor provision in the Trade Expansion Act warrants a review of its requirements and procedures. This Article illustrates those requirements and procedures through § 301\u27s application in particular cases. The Article also explains why recent events have led to more frequent resort to § 301 and related legal provisions. Section 301 is currently the lodestar of the Administration\u27s free and fair trade policy. This policy stresses, in particular, the need for equitable access to foreign markets for United States exports of goods and services and foreign direct investment. The increasing importance of § 301 is evident from the range and significance of actions which have been taken recently. For these reasons, an understanding of the legal criteria for action under § 301 and the procedures employed in these proceedings is critical to the effective use of available United States trade remedies

High-resolution spectroscopy of triplet states of Rb2 by femtosecond pump-probe photoionization of doped helium nanodroplets

The dynamics of vibrational wave packets in triplet states of rubidium dimers (Rb2) formed on helium nanodroplets are studied using femtosecond pump-probe photoionization spectroscopy. Due to fast desorption of the excited Rb2 molecules off the droplets and due to their low internal temperature, wave packet oscillations can be followed up to very long pump-probe delay times >1.5ns. In the first excited triplet state (1)^3\Sigma_g^+, full and fractional revivals are observed with high contrast. Fourier analysis provides high-resolution vibrational spectra which are in excellent agreement with ab initio calculations

Cavity-Enhanced Raman Spectroscopy in the Biosciences: In situ, Multicomponent and Isotope Selective Gas Measurements to Study Hydrogen Production and Consumption by Escherichia coli

Recently we introduced cavity-enhanced Raman spectroscopy (CERS) with optical feedback cw-diode lasers as a sensitive analytical tool. Here we report improvements made on the technique and its first application in the biosciences for in situ, multicomponent, and isotope selective gas measurements to study hydrogen production and consumption by Escherichia coli. Under anaerobic conditions, cultures grown on rich media supplemented with D-glucose or glycerol produce H2 and simultaneously consume some of it. By introducing D2 in the headspace, hydrogen production and consumption could be separated due to the distinct spectroscopic signatures of isotopomers. Different phases with distinctly different kinetic regimes of H2 and CO2 production and D2 consumption were identified. Some of the D2 consumed is converted back to H2 via H/D exchange with the solvent. HD was formed only as a minor component. This reflects either that H/D exchange at hydrogenase active sites is rapid compared to the rate of recombination, rapid recapture of HD occurs after the molecule is formed, or that the active sites where D2 oxidation and proton reduction occur are physically separated. Whereas in glucose supplemented cultures, addition of D2 led to an increase in H2 produced, while the yield of CO2 remained unchanged; with glycerol, addition of D2 led not only to increased yields of H2, but also significantly increased CO2 production, reflecting an impact on fermentation pathways. Addition of CO was found to completely inhibit H2 production and significantly reduce D2 oxidation, indicating at least some role for O2-tolerant Hyd-1 in D2 consumption

The Impact of Adaptive Optics on Star Formation Research

In this paper, we discuss the benefits of ground-based, adaptive optics (AO) aided observations for star formation research. After outlining the general advantages, we present results obtained during the ALFA science demonstration programme in 1999. These results underline the absolute necessity of AO assistance for almost any kind of observations regarding star formation regions.Comment: 10 pages, 8 figures (partially converted to bitmap), to appear in SPIE Proceedings 4007, Astronomical Telescopes and Instrumentation 2000, Munich, German

Proposal for International Agreement on Ca Notation resulting from discussion at workshops on stable isotope measurement held in Davos (Goldschmidt 2002) and Nice (EGS-AGU-EUG 2003)

A proposal is made to standardise the reporting of Ca isotope data to the δ44Ca/40Ca notation (or δ44Ca/42Ca) and to adopt NIST SRM 915a as the reference standard

Psychological sources of response effects in self-administered and telephone surveys

The impact of mode of data collection (self-administered questionnaire vs. telephone interview) on the emergence of response effects and the accuracy of recall from memory was explored in a cross-cultural experiment, conducted in the U.S. and the Federal Republic of Germany. As predicted on the basis of psychological considerations, question order effects were obtained under telephone interview conditions but not under self-administered conditions, where question order is eliminated by the opportunity to browse back and forth through the questionnaire. On the other hand, the impact of the content of related questions was more pronounced under self-administered than under telephone interview conditions, independent of the order in which they were presented. This reflects respondents' differential opportunity to elaborate on related questions under both administration modes, as well as the necessity to rely on the content of presumably related questions in determining the meaning of ambiguous questions under self-administered conditions. Finally, respondents' recall of the date of public events was more accurate under self-administered than under telephone interview conditions, reflecting the beneficial effect of having sufficient time to work on the recall task

Hydrogen production in the presence of oxygen by Escherichia coli K-12

Escherichia coli is a facultative anaerobe that can grow in a variety of environmental conditions. In the complete absence of O2, E. coli can perform a mixed-acid fermentation that contains within it an elaborate metabolism of formic acid. In this study, we use cavity-enhanced Raman spectroscopy (CERS), FTIR, liquid Raman spectroscopy, isotopic labelling and molecular genetics to make advances in the understanding of bacterial formate and H2 metabolism. It is shown that, under anaerobic (anoxic) conditions, formic acid is generated endogenously, excreted briefly from the cell, and then taken up again to be disproportionated to H2 and CO2 by formate hydrogenlyase (FHL-1). However, exogenously added D-labelled formate behaves quite differently from the endogenous formate and is taken up immediately, independently, and possibly by a different mechanism, by the cell and converted to H2 and CO2. Our data support an anion-proton symport model for formic acid transport. In addition, when E. coli was grown in a micro-aerobic (micro-oxic) environment it was possible to analyse aspects of formate and O2 respiration occurring alongside anaerobic metabolism. While cells growing under micro-aerobic conditions generated endogenous formic acid, no H2 was produced. However, addition of exogenous formate at the outset of cell growth did induce FHL-1 biosynthesis and resulted in formate-dependent H2 production in the presence of O2