« Myectome » et « Bloc paraffiné » (Comparaison)

Lucam François, Flachat Ch., Gangloff L., Boitel J.-C. « Myectome » et « Bloc paraffiné » (Comparaison). In: Bulletin de l'Académie Vétérinaire de France tome 109 n°5, 1956. pp. 239-248

SIRTA, a ground-based atmospheric observatory for cloud and aerosol research

Ground-based remote sensing observatories have a crucial role to play in providing data to improve our understanding of atmospheric processes, to test the performance of atmospheric models, and to develop new methods for future space-borne observations. Institut Pierre Simon Laplace, a French research institute in environmental sciences, created the Site Instrumental de Recherche par Télédétection Atmosphérique (SIRTA), an atmospheric observatory with these goals in mind. Today SIRTA, located 20km south of Paris, operates a suite a state-of-the-art active and passive remote sensing instruments dedicated to routine monitoring of cloud and aerosol properties, and key atmospheric parameters. Detailed description of the state of the atmospheric column is progressively archived and made accessible to the scientific community. This paper describes the SIRTA infrastructure and database, and provides an overview of the scientific research associated with the observatory. Researchers using SIRTA data conduct research on atmospheric processes involving complex interactions between clouds, aerosols and radiative and dynamic processes in the atmospheric column. Atmospheric modellers working with SIRTA observations develop new methods to test their models and innovative analyses to improve parametric representations of sub-grid processes that must be accounted for in the model. SIRTA provides the means to develop data interpretation tools for future active remote sensing missions in space (e.g. CloudSat and CALIPSO). SIRTA observation and research activities take place in networks of atmospheric observatories that allow scientists to access consistent data sets from diverse regions on the globe

Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans.

Collaboration with: Université d’Orléans, 28000 Chartres, France, Université de Picardie Jules Verne, F-80037 Amiens, France De Montfort University Open access articleLinum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands

Sub-lethal effects of waterborne exposure to copper nanoparticles compared to copper sulphate on the shore crab ( Carcinus maenas )

publisher: Elsevier articletitle: Sub-lethal effects of waterborne exposure to copper nanoparticles compared to copper sulphate on the shore crab (Carcinus maenas) journaltitle: Aquatic Toxicology articlelink: http://dx.doi.org/10.1016/j.aquatox.2017.08.006 content_type: article copyright: © 2017 Elsevier B.V. All rights reserved

Optimisation of Brassica rapa rapa (turnip) hairy root culture media for the production of biomass and secretion of recalcitrant recombinant proteins

The increasing demand for recombinant proteins used as therapeutic agents has influenced the development of different production platforms. Plant systems are attractive for recombinant protein production as they offer safety (no risk of human-threatening viral contamination), low upstream costs, complex glycosylation, and high scalability. Among the plant-based systems, hairy root cultures present several advantages such as rapid propagation, genotypic and phenotypic stability, and among others, possible extracellular secretion of expressed proteins in a well-defined medium. The optimization of the hairy root cultures to produce sufficient yields of secreted and easy-to-purify recombinant proteins is an on-going endeavor as it usually depends on the protein of interest. As part of the H2020 PharmaFactory project, several strategies are being assessed to maximize the productivity of “hard-to-produce” recombinant proteins in hairy root cultures. In this regard, we are evaluating the impact of selected culture medium agents on productivity. The aim is to identify, from a range of culture medium additives, those that when used in combination or alone, increase the productivity of recalcitrant recombinant proteins. The potential of such agents has been evaluated based on statistical experimental design. Subsequently, with the biomass values and recombinant protein yields retrieved, a model is created to predict culture media conditions giving rise to the best recombinant protein yield. Currently, sucrose and calcium chloride have demonstrated to be important for the biomass production. More research is still required in order to create models with a higher prediction power