Expression and localization of glutamate-gated chloride channel variants in honeybee brain (Apis mellifera)

Due to its specificity to invertebrate species, glutamate-gated chloride channels (GluCls) are the target sites of antiparasitic agents and insecticides, e.g. ivermectin and fipronil, respectively. In nematodes and insects, the GluCls diversity is broadened by alternative splicing. GluCl subunits have been characterized according to their sensitivity to drugs, and to their anatomical localization. In the honeybee, the GluCl gene can encode different alpha subunits due to alternative splicing of exon 3. We examined mRNA expression in brain parts and we confirmed the existence of two GluCl variants with RT-PCR, Amel_GluCl A and Amel_GluCl B. Surprisingly, a mixed isoform not yet described in insect was obtained, we called it Amel_GluCl C. We determined precise immunolocalization of peptide sequence corresponding to Amel_GluCl A and Amel_GluCl B in the honeybee brain. Amel_GluCl A is mainly located in neuropils, whereas Amel_GluCl B is mostly expressed in cell bodies. Both proteins can also be co-localized. According to their anatomical localization, different GluCl variants might be involved in olfactory and visual modalities and in learning and memory

The IAXO Helioscope

Çetin, Serkant Ali (Dogus Author) -- Conference full title: 7th International Symposium on Large TPCs for Low-Energy Rare Event Detection; Institute of Astroparticle Physics (APC) Campus - Paris Diderot UniversityParis; France; 15 December 2014 through 17 December 2014The IAXO (International Axion Experiment) is a fourth generation helioscope with a sensitivity, in terms of detectable signal counts, at least 104 better than CAST phase-I, resulting in sensitivity on gaγ one order of magnitude better. To achieve this performance IAXO will count on a 8-coil toroidal magnet with 60 cm diameter bores and equipped with X-ray focusing optics into 0.20 cm2 spots coupled to ultra-low background Micromegas X-ray detectors. The magnet will be on a platform that will allow solar tracking for 12 hours per day. The next short term objectives are to prepare a Technical Design Report and to construct the first prototypes of the hardware main ingredients: demonstration coil, X-ray optics and low background detector while refining the physics case and studying the feasibility studies for Dark Matter axions

The Eleventh and Twelfth Data Releases of the Sloan Digital Sky Survey: Final Data from SDSS-III

The third generation of the Sloan Digital Sky Survey (SDSS-III) took data from 2008 to 2014 using the original SDSS wide-field imager, the original and an upgraded multi-object fiber-fed optical spectrograph, a new near-infrared high-resolution spectrograph, and a novel optical interferometer. All of the data from SDSS-III are now made public. In particular, this paper describes Data Release 11 (DR11) including all data acquired through 2013 July, and Data Release 12 (DR12) adding data acquired through 2014 July (including all data included in previous data releases), marking the end of SDSS-III observing. Relative to our previous public release (DR10), DR12 adds one million new spectra of galaxies and quasars from the Baryon Oscillation Spectroscopic Survey (BOSS) over an additional 3000 deg2 of sky, more than triples the number of H-band spectra of stars as part of the Apache Point Observatory (APO) Galactic Evolution Experiment (APOGEE), and includes repeated accurate radial velocity measurements of 5500 stars from the Multi-object APO Radial Velocity Exoplanet Large-area Survey (MARVELS). The APOGEE outputs now include the measured abundances of 15 different elements for each star. In total, SDSS-III added 5200 deg2 of ugriz imaging; 155,520 spectra of 138,099 stars as part of the Sloan Exploration of Galactic Understanding and Evolution 2 (SEGUE-2) survey; 2,497,484 BOSS spectra of 1,372,737 galaxies, 294,512 quasars, and 247,216 stars over 9376 deg2; 618,080 APOGEE spectra of 156,593 stars; and 197,040 MARVELS spectra of 5513 stars. Since its first light in 1998, SDSS has imaged over 1/3 of the Celestial sphere in five bands and obtained over five million astronomical spectra

The Pierre Auger Observatory: Results on Ultra-High Energy Cosmic Rays

The focus of this article is on recent results on ultra-high energy cosmic rays obtained with the Pierre Auger Observatory. The world's largest instrument of this type and its performance are described. The observations presented here include the energy spectrum, the primary particle composition, limits on the fluxes of photons and neutrinos and a discussion of the anisotropic distribution of the arrival directions of the most energetic particles. Finally, plans for the construction of a Northern Auger Observatory in Colorado, USA, are discussed.Comment: Proceedings of the International Workshop on Advances in Cosmic Ray Science, Waseda University, Shinjuku, Tokyo, Japan, March 2008; to be published in the Journal of the Physical Society of Japan (JPSJ) supplemen

THE ELEVENTH AND TWELFTH DATA RELEASES OF THE SLOAN DIGITAL SKY SURVEY: FINAL DATA FROM SDSS-III

Citation: Alam, S., Albareti, F. D., Prieto, C. A., Anders, F., Anderson, S. F., Anderton, T., . . . Zhu, G. T. (2015). THE ELEVENTH AND TWELFTH DATA RELEASES OF THE SLOAN DIGITAL SKY SURVEY: FINAL DATA FROM SDSS-III. Astrophysical Journal Supplement Series, 219(1), 27. doi:10.1088/0067-0049/219/1/12The third generation of the Sloan Digital Sky Survey (SDSS-III) took data from 2008 to 2014 using the original SDSS wide-field imager, the original and an upgraded multi-object fiber-fed optical spectrograph, a new near-infrared high-resolution spectrograph, and a novel optical interferometer. All of the data from SDSS-III are now made public. In particular, this paper describes Data Release 11 (DR11) including all data acquired through 2013 July, and Data Release 12 (DR12) adding data acquired through 2014 July (including all data included in previous data releases), marking the end of SDSS-III observing. Relative to our previous public release (DR10), DR12 adds one million new spectra of galaxies and quasars from the Baryon Oscillation Spectroscopic Survey (BOSS) over an additional 3000 deg(2) of sky, more than triples the number of H-band spectra of stars as part of the Apache Point Observatory (APO) Galactic Evolution Experiment (APOGEE), and includes repeated accurate radial velocity measurements of 5500 stars from the Multi-object APO Radial Velocity Exoplanet Large-area Survey (MARVELS). The APOGEE outputs now include the measured abundances of 15 different elements for each star. In total, SDSS-III added 5200 deg(2) of ugriz imaging; 155,520 spectra of 138,099 stars as part of the Sloan Exploration of Galactic Understanding and Evolution 2 (SEGUE-2) survey; 2,497,484 BOSS spectra of 1,372,737 galaxies, 294,512 quasars, and 247,216 stars over 9376 deg(2); 618,080 APOGEE spectra of 156,593 stars; and 197,040 MARVELS spectra of 5513 stars. Since its first light in 1998, SDSS has imaged over 1/3 of the Celestial sphere in five bands and obtained over five million astronomical spectra.Additional Authors: Berlind, A. A.;Beutler, F.;Bhardwaj, V.;Bird, J. C.;Bizyaev, D.;Blake, C. H.;Blanton, M. R.;Blomqvist, M.;Bochanski, J. J.;Bolton, A. S.;Bovy, J.;Bradley, A. S.;Brandt, W. N.;Brauer, D. E.;Brinkmann, J.;Brown, P. J.;Brownstein, J. R.;Burden, A.;Burtin, E.;Busca, N. G.;Cai, Z.;Capozzi, D.;Rosell, A. C.;Carr, M. A.;Carrera, R.;Chambers, K. C.;Chaplin, W. J.;Chen, Y. C.;Chiappini, C.;Chojnowski, S. D.;Chuang, C. H.;Clerc, N.;Comparat, J.;Covey, K.;Croft, R. A. C.;Cuesta, A. J.;Cunha, K.;da Costa, L. N.;Da Rio, N.;Davenport, J. R. A.;Dawson, K. S.;De Lee, N.;Delubac, T.;Deshpande, R.;Dhital, S.;Dutra-Ferreira, L.;Dwelly, T.;Ealet, A.;Ebelke, G. L.;Edmondson, E. M.;Eisenstein, D. J.;Ellsworth, T.;Elsworth, Y.;Epstein, C. R.;Eracleous, M.;Escoffier, S.;Esposito, M.;Evans, M. L.;Fan, X. H.;Fernandez-Alvar, E.;Feuillet, D.;Ak, N. F.;Finley, H.;Finoguenov, A.;Flaherty, K.;Fleming, S. W.;Font-Ribera, A.;Foster, J.;Frinchaboy, P. M.;Galbraith-Frew, J. G.;Garcia, R. A.;Garcia-Hernandez, D. A.;Perez, A. E. G.;Gaulme, P.;Ge, J.;Genova-Santos, R.;Georgakakis, A.;Ghezzi, L.;Gillespie, B. A.;Girardi, L.;Goddard, D.;Gontcho, S. G. A.;Hernandez, J. I. G.;Grebel, E. K.;Green, P. J.;Grieb, J. N.;Grieves, N.;Gunn, J. E.;Guo, H.;Harding, P.;Hasselquist, S.;Hawley, S. L.;Hayden, M.;Hearty, F. R.;Hekker, S.;Ho, S.;Hogg, D. W.;Holley-Bockelmann, K.;Holtzman, J. A.;Honscheid, K.;Huber, D.;Huehnerhoff, J.;Ivans, II;Jiang, L. H.;Johnson, J. A.;Kinemuchi, K.;Kirkby, D.;Kitaura, F.;Klaene, M. A.;Knapp, G. R.;Kneib, J. P.;Koenig, X. P.;Lam, C. R.;Lan, T. W.;Lang, D. T.;Laurent, P.;Le Goff, J. M.;Leauthaud, A.;Lee, K. G.;Lee, Y. S.;Licquia, T. C.;Liu, J.;Long, D. C.;Lopez-Corredoira, M.;Lorenzo-Oliveira, D.;Lucatello, S.;Lundgren, B.;Lupton, R. H.;Mack, C. E.;Mahadevan, S.;Maia, M. A. G.;Majewski, S. R.;Malanushenko, E.;Malanushenko, V.;Manchado, A.;Manera, M.;Mao, Q. Q.;Maraston, C.;Marchwinski, R. C.;Margala, D.;Martell, S. L.;Martig, M.;Masters, K. L.;Mathur, S.;McBride, C. K.;McGehee, P. M.;McGreer, I. D.;McMahon, R. G.;Menard, B.;Menzel, M. L.;Merloni, A.;Meszaros, S.;Miller, A. A.;Miralda-Escude, J.;Miyatake, H.;Montero-Dorta, A. D.;More, S.;Morganson, E.;Morice-Atkinson, X.;Morrison, H. L.;Mosser, B.;Muna, D.;Myers, A. D.;Nandra, K.;Newman, J. A.;Neyrinck, M.;Nguyen, D. C.;Nichol, R. C.;Nidever, D. L.;Noterdaeme, P.;Nuza, S. E.;O'Connell, J. E.;O'Connell, R. W.;O'Connell, R.;Ogando, R. L. C.;Olmstead, M. D.;Oravetz, A. E.;Oravetz, D. J.;Osumi, K.;Owen, R.;Padgett, D. L.;Padmanabhan, N.;Paegert, M.;Palanque-Delabrouille, N.;Pan, K. K.;Parejko, J. K.;Paris, I.;Park, C.;Pattarakijwanich, P.;Pellejero-Ibanez, M.;Pepper, J.;Percival, W. J.;Perez-Fournon, I.;Perez-Rafols, I.;Petitjean, P.;Pieri, M. M.;Pinsonneault, M. H.;de Mello, G. F. P.;Prada, F.;Prakash, A.;Price-Whelan, A. M.;Protopapas, P.;Raddick, M. J.;Rahman, M.;Reid, B. A.;Rich, J.;Rix, H. W.;Robin, A. C.;Rockosi, C. M.;Rodrigues, T. S.;Rodriguez-Torres, S.;Roe, N. A.;Ross, A. J.;Ross, N. P.;Rossi, G.;Ruan, J. J.;Rubino-Martin, J. A.;Rykoff, E. S.;Salazar-Albornoz, S.;Salvato, M.;Samushia, L.;Sanchez, A. G.;Santiago, B.;Sayres, C.;Schiavon, R. P.;Schlegel, D. J.;Schmidt, S. J.;Schneider, D. P.;Schultheis, M.;Schwope, A. D.;Scoccola, C. G.;Scott, C.;Sellgren, K.;Seo, H. J.;Serenelli, A.;Shane, N.;Shen, Y.;Shetrone, M.;Shu, Y. P.;Aguirre, V. S.;Sivarani, T.;Skrutskie, M. F.;Slosar, A.;Smith, V. V.;Sobreira, F.;Souto, D.;Stassun, K. G.;Steinmetz, M.;Stello, D.;Strauss, M. A.;Streblyanska, A.;Suzuki, N.;Swanson, M. E. C.;Tan, J. C.;Tayar, J.;Terrien, R. C.;Thakar, A. R.;Thomas, D.;Thomas, N.;Thompson, B. A.;Tinker, J. L.;Tojeiro, R.;Troup, N. W.;Vargas-Magana, M.;Vazquez, J. A.;Verde, L.;Viel, M.;Vogt, N. P.;Wake, D. A.;Wang, J.;Weaver, B. A.;Weinberg, D. H.;Weiner, B. J.;White, M.;Wilson, J. C.;Wisniewski, J. P.;Wood-Vasey, W. M.;Yeche, C.;York, D. G.;Zakamska, N. L.;Zamora, O.;Zasowski, G.;Zehavi, I.;Zhao, G. B.;Zheng, Z.;Zhou, X.;Zhou, Z. M.;Zou, H.;Zhu, G. T

The Deinococcus radiodurans DR1245 Protein, a DdrB Partner Homologous to YbjN Proteins and Reminiscent of Type III Secretion System Chaperones

The bacterium Deinococcus radiodurans exhibits an extreme resistance to ionizing radiation. A small subset of Deinococcus genus-specific genes were shown to be up-regulated upon exposure to ionizing radiation and to play a role in genome reconstitution. These genes include an SSB-like protein called DdrB. Here, we identified a novel protein encoded by the dr1245 gene as an interacting partner of DdrB. A strain devoid of the DR1245 protein is impaired in growth, exhibiting a generation time approximately threefold that of the wild type strain while radioresistance is not affected. We determined the three-dimensional structure of DR1245, revealing a relationship with type III secretion system chaperones and YbjN family proteins. Thus, DR1245 may display some chaperone activity towards DdrB and possibly other substrates. © 2013 Norais et al

A search for low-mass WIMPs with EDELWEISS-II heat-and-ionization detectors

We report on a search for low-energy (E < 20 keV) WIMP-induced nuclear recoils using data collected in 2009 - 2010 by EDELWEISS from four germanium detectors equipped with thermal sensors and an electrode design (ID) which allows to efficiently reject several sources of background. The data indicate no evidence for an exponential distribution of low-energy nuclear recoils that could be attributed to WIMP elastic scattering after an exposure of 113 kg.d. For WIMPs of mass 10 GeV, the observation of one event in the WIMP search region results in a 90% CL limit of 1.0x10^-5 pb on the spin-independent WIMP-nucleon scattering cross-section, which constrains the parameter space associated with the findings reported by the CoGeNT, DAMA and CRESST experiments.Comment: PRD rapid communication accepte

Deciphering neuronal deficit and protein profile changes in human brain organoids from patients with creatine transporter deficiency

Creatine transporter deficiency (CTD) is an X-linked disease caused by mutations in the SLC6A8 gene. The impaired creatine uptake in the brain results in intellectual disability, behavioral disorders, language delay, and seizures. In this work, we generated human brain organoids from induced pluripotent stem cells of healthy subjects and CTD patients. Brain organoids from CTD donors had reduced creatine uptake compared with those from healthy donors. The expression of neural progenitor cell markers SOX2 and PAX6 was reduced in CTD-derived organoids, while GSK3β, a key regulator of neurogenesis, was up-regulated. Shotgun proteomics combined with integrative bioinformatic and statistical analysis identified changes in the abundance of proteins associated with intellectual disability, epilepsy, and autism. Re-establishment of the expression of a functional SLC6A8 in CTD-derived organoids restored creatine uptake and normalized the expression of SOX2, GSK3β, and other key proteins associated with clinical features of CTD patients. Our brain organoid model opens new avenues for further characterizing the CTD pathophysiology and supports the concept that reinstating creatine levels in patients with CTD could result in therapeutic efficacy