Nucleotide specificity of the enzymatic and motile activities of dynein, kinesin, and heavy meromyosin.

The substrate specificities of dynein, kinesin, and myosin substrate turnover activity and cytoskeletal filament-driven translocation were examined using 15 ATP analogues. The dyneins were more selective in their substrate utilization than bovine brain kinesin or muscle heavy meromyosin, and even different types of dyneins, such as 14S and 22S dynein from Tetrahymena cilia and the beta-heavy chain-containing particle from the outer-arm dynein of sea urchin flagella, could be distinguished by their substrate specificities. Although bovine brain kinesin and muscle heavy meromyosin both exhibited broad substrate specificities, kinesin-induced microtubule translocation varied over a 50-fold range in speed among the various substrates, whereas heavy meromyosin-induced actin translocation varied only by fourfold. With both kinesin and heavy meromyosin, the relative velocities of filament translocation did not correlate well with the relative filament-activated substrate turnover rates. Furthermore, some ATP analogues that did not support the filament translocation exhibited filament-activated substrate turnover rates. Filament-activated substrate turnover and power production, therefore, appear to become uncoupled with certain substrates. In conclusion, the substrate specificities and coupling to motility are distinct for different types of molecular motor proteins. Such nucleotide "fingerprints" of enzymatic activities of motor proteins may prove useful as a tool for identifying what type of motor is involved in powering a motility-related event that can be reconstituted in vitro

DC/DC pretvarači za motorne pogone – koncept, dizajn i unaprijedno upravljanje

Solar photovoltaics is one of the most promising technologies for the non-carbon based electricity production. Power electronics is therefore necessary. In this paper a converter is analyzed which makes it possible to control the mean value of the output voltage, this is e.g. the voltage across a DC machine (and therefore the speed) from zero up to three times of the input voltage. The converter is also useful for charging batteries. The model of the drive, the design of the devices, the control features are discussed, feed-forward control based on solving the limit cycle is designed, some experimental results, and hints for the design are given.Solarni paneli jedni su od najznačajnijih sustava za proizvodnju električne energije koja se ne temelji na ugljenu. U te svrhe nužno je korištenje učinske elektronike. U ovome radu analiziran je ispravljač koji omogućuje upravljanje srednjom vrijednosti izlaznog napona, tj. napona u odnosu na DC uređaj (stoga i upravljanje brzinom) u rasponu od nule pa do tri puta razine izlaznog napona. Ispravljač se također može koristiti za punjenje baterija. Model i dizajn uređaja zajedno sa sustavom upravljanja opisani su u radu. Prikazano je unaprijedno upravljanje temeljeno na rješavanju graničnih ciklusa, dani su eksperimentalni rezultati te su prikazane pojedinosti dizajniranja uređaja

Superfluid transition temperature in a trapped gas of Fermi atoms with a Feshbach resonance

We investigate strong coupling effects on the superfluid phase transition in a gas of Fermi atoms with a Feshbach resonance. The Feshbach resonance describes a composite quasi-Boson, which can give rise to an additional pairing interaction between the Fermi atoms. This attractive interaction becomes stronger as the threshold energy of the Feshbach resonance two-particle bound state is lowered. In a recent paper, we showed that in the uniform Fermi gas, this tunable pairing interaction naturally leads to a BCS-BEC crossover of the Nozi`eres and Schmitt-Rink kind, in which the BCS-type superfluid phase transition continuously changes into the BEC-type as the threshold energy is decreased. In this paper, we extend our previous work by including the effect of a harmonic trap potential, treated within the local density approximation (LDA). We also give results for both weak and strong coupling to the Feshbach resonance. We show that the BCS-BEC crossover phenomenon strongly modifies the shape of the atomic density profile at the superfluid phase transition temperature Tc, reflecting the change of the dominant particles going from Fermi atoms to composite Bosons. In the BEC regime, these composite Bosons are shown to first appear well above Tc. We also discuss the "phase diagram" above Tc as a function of the tunable threshold energy. We introduce a characteristic temperature T* describing the effective crossover in the normal phase from a Fermi gas of atoms to a gas of stable molecules.Comment: 43 pages, 13 figures (submitted to PRA

Andreev reflections on Y1-xCaxBa2Cu3O7-delta evidence for an unusual proximity effect

We have measured Andreev reflections between an Au tip and Y_{1-x}Ca_{x}Ba_{2}Cu_{3}O_{7 - \delta} thin films in the in-plane orientation. The conductance spectra are best fitted with a pair potential having the "d_{x^{2}-y^{2}+is" symmetry. We find that the amplitude of the "is" component is enhanced as the contact transparency is increased. This is an indication for an unusual proximity effect that modifies the pair potential in the superconductor near the surface with the normal metal.Comment: 4 pages, 4 figure

Two Dimensional Electron and Hole Gases at the Surface of Graphite

We report high-quality two-dimensional (2D) electron and hole gases induced at the surface of graphite by the electric field effect. The 2D carriers reside within a few near-surface atomic layers and exhibit mobilities up to 15,000 and 60,000 cm2/Vs at room and liquid-helium temperatures, respectively. The mobilities imply ballistic transport at micron scale. Pronounced Shubnikov-de Haas oscillations reveal the existence of two types of carries in both 2D electron and hole gases.Comment: related to cond-mat/0410631 where preliminary data for this experimental system were reporte

Avaliação de acessos de milho para resistência a Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) em laboratório.

Avaliou-se uma coleção de milho para resistência à lagarta de Spodoptera frugiperda (J.E.Smith) (Lepidóptera: Noctuidae) em testes de laboratório da UFRA. Utilizou-se 25 acessos de milho proveniente do banco de germoplasma da Embrapa Milho e Sorgo, em um experimento inteiramente ao acaso com três repetições. Observou-se diariamente o desenvolvimento dos insetos durante as fases de larva e pupa, para registrar as alterações biológicas promovidas pelos acessos de milho. Os dados observados foram submetidos à ANOVA, as médias foram comparadas pelo teste SNK e através da análise de regressão linear, determinou-se o grau de dependência entre o consumo foliar de S. frugiperda na fase larval e a respectiva biomassa do inseto na fase pupal. Os resultados mostraram que houve influência dos acessos de milho sobre o desenvolvimento da lagarta-do-cartucho do milho. Os acessos que promoveram a menor percentagem de viabilidade de lagartas foram AM 013, RO 009 e MA 002, enquanto RR 168 e PA 110 foram os menos consumidos pelas lagartas de S. frugiperda

Kondo effect in two-dimensional disordered electron systems

We investigate the Kondo effect in two-dimensional disordered electron systems using a finite-temperature quantum Monte Carlo method. Depending on the position of a magnetic impurity, the local moment is screened or unscreened by the spin of the conduction electron. On the basis of the results, we show that the distribution of the Kondo temperature becomes wide and the weight at $T_K=0$ becomes large as randomness increases. The average susceptibility shows a weak power-law or logarithmic divergence at low temperature, indicating a non-Fermi-liquid behavior.Comment: 2 pages, 2 figures, to be published in supplement of J. Phys. Soc. Japan, Proceedings of Localisation 2002, (Tokyo, Japan, 2002

Effective action approach and Carlson-Goldman mode in d-wave superconductors

We theoretically investigate the Carlson-Goldman (CG) mode in two-dimensional clean d-wave superconductors using the effective ``phase only'' action formalism. In conventional s-wave superconductors, it is known that the CG mode is observed as a peak in the structure factor of the pair susceptibility $S(\Omega, \mathbf{K})$ only just below the transition temperature T_c and only in dirty systems. On the other hand, our analytical results support the statement by Y.Ohashi and S.Takada, Phys.Rev.B {\bf 62}, 5971 (2000) that in d-wave superconductors the CG mode can exist in clean systems down to the much lower temperatures, $T \approx 0.1 T_c$. We also consider the manifestations of the CG mode in the density-density and current-current correlators and discuss the gauge independence of the obtained results.Comment: 23 pages, RevTeX4, 12 EPS figures; final version to appear in PR

Studying the WHIM with Gamma Ray Bursts

We assess the possibility to detect and characterize the physical state of the missing baryons at low redshift by analyzing the X-ray absorption spectra of the Gamma Ray Burst [GRB] afterglows, measured by a micro calorimeters-based detector with 3 eV resolution and 1000 cm2 effective area and capable of fast re-pointing, similar to that on board of the recently proposed X-ray satellites EDGE and XENIA. For this purpose we have analyzed mock absorption spectra extracted from different hydrodynamical simulations used to model the properties of the Warm Hot Intergalactic Medium [WHIM]. These models predict the correct abundance of OVI absorption lines observed in UV and satisfy current X-ray constraints. According to these models space missions like EDGE and XENIA should be able to detect about 60 WHIM absorbers per year through the OVII line. About 45 % of these have at least two more detectable lines in addition to OVII that can be used to determine the density and the temperature of the gas. Systematic errors in the estimates of the gas density and temperature can be corrected for in a robust, largely model-independent fashion. The analysis of the GRB absorption spectra collected in three years would also allow to measure the cosmic mass density of the WHIM with about 15 % accuracy, although this estimate depends on the WHIM model. Our results suggest that GRBs represent a valid, if not preferable, alternative to Active Galactic Nuclei to study the WHIM in absorption. The analysis of the absorption spectra nicely complements the study of the WHIM in emission that the spectrometer proposed for EDGE and XENIA would be able to carry out thanks to its high sensitivity and large field of view.Comment: 16 pages, 16 figures, accepted for publication by Ap

Spectral Evolution of Circinus X-1 Along its Orbit

We report on the spectral analysis of Circinus X-1 observed by the ASCA satellite in March 1998 along one orbital period. The luminosity of the source (in the 0.1-100 keV band) ranges from $2.5 \times 10^{38}$ erg s$^{-1}$ at the periastron (orbital phase 0.01) to $1.5 \times 10^{38}$ erg s$^{-1}$ at orbital phase 0.3. From the spectral analysis and the lightcurve we argue that Cir X-1 shows three states along the orbital evolution. The first state is at the orbital phase interval 0.97-0.3: the luminosity becames super-Eddington and a strong flaring activity is present. In this state a shock could form in the inner region of the system due to the super-Eddington accretion rate, producing an outflow of ionized matter whose observational signature could be the prominent absorption edge at $\sim 8.7$ keV observed in the energy spectrum at these phases. In the second state, corresponding to the orbital phase interval between 0.3 and 0.7, the accretion rate is sub-Eddington and we observe a weaker outflow, with smaller hydrogen column: the absorption edge is now at $\sim 8.3$ keV with an optical depth a factor of 2.5 to 6 smaller. The third state corresponds to the orbital phase interval 0.78-0.97. In this state the best fit model to the spectrum requires the presence of a partial covering component, indicating that the emission from the compact object is partially absorbed by neutral matter, probably the atmosphere of the companion star and/or the accreting matter from the companion.Comment: 18 pages, 3 figures. Accepted by Ap