Ward's Hierarchical Clustering Method: Clustering Criterion and Agglomerative Algorithm

The Ward error sum of squares hierarchical clustering method has been very widely used since its first description by Ward in a 1963 publication. It has also been generalized in various ways. However there are different interpretations in the literature and there are different implementations of the Ward agglomerative algorithm in commonly used software systems, including differing expressions of the agglomerative criterion. Our survey work and case studies will be useful for all those involved in developing software for data analysis using Ward's hierarchical clustering method.Comment: 20 pages, 21 citations, 4 figure

A human factors evaluation of the robotic interface for Space Station Freedom orbital replaceable units

An orbital replaceable unit (ORU) is often defined as any orbital unit aboard Space Station with a wearout life of less than 30 years. The capability of successful changeout of these units by remote manipulation is critical to the ORU to telerobot interface design. A human factors evaluation of the selected interface showed certain inadequacies of the alignment target concept that was part of the interface package. Alternative target concepts which addressed these inadequacies were developed and are presented. Recommendations will be incorporated into NASA requirements documents which ORU suppliers and manufacturers must then build to

Currents under land-fast ice in the Canadian Arctic Archipelago Part 2: Vertical mixing

During an acoustic Doppler current profiler (ADCP) study of the velocity field under land-fast ice, coincident CTD casts showed three instances of pronounced instability in the water column, immediately above and below the pycnocline. In this paper we demonstrate that the density inversions are associated with the passage of high frequency linear internal waves and finite amplitude waves. Contours of ADCP acoustic return intensity display pronounced vertical eddy-like features which may indicate overturning. A Richardson number calculation showed that even the most highly stratified portion of the pycnocline had sufficient vertical velocity shear to promote dynamic instability. Finally, we calculate that the vertical nutrient flux, resulting from the high frequency internal wave field, could supply a significant portion of the ice-algae nutrient budget

Transitional random matrix theory nearest-neighbor spacing distributions

This paper presents a study of the properties of a matrix model that was introduced to describe transitions between all Wigner surmises of Random Matrix theory. New results include closed-form exact analytical expressions for the transitional probability density functions, as well as suitable analytical approximations for cases not amenable to explicit representation

Long-term changes in a zooplankton community revealed by the sediment archive

To reconstruct changes in zooplankton communities in response to past anthropogenic perturbations, one possibility is to use the sedimentary records. We analyzed the sediments at a coastal site in the Northern Baltic Sea to relate changes in the zooplankton community to anthropogenic eutrophication and the invasion of a predatory cladoceran, Cercopagis pengoi. We sampled 30-cm laminated sediment cores and dated the sediment layers back to the 1950s. From each 1-cm layer, we measured eutrophication indicators (delta C-13, delta N-15, TC, TN, TP) and identified and counted zooplankton resting eggs (cladoceran, calanoid copepod, rotifer). In addition, we estimated the abundance of the cladoceran Bosmina (Eubosmina) maritima by counting subfossils (carapaces, headshields, and ephippia) and estimated the experienced stress as the relationship between sexual and asexual reproduction. Using redundancy and variance partitioning analyses, we found similar to 16% of the variation in the zooplankton community to be explained by eutrophication, and 24% of the variation in B. (E.) maritima abundance and reproduction mode to be explained by eutrophication and the introduction of the alien predator. Our results show a long-term shift from calanoid copepods and predatory cladocerans toward small-sized zooplankton species, like rotifers. Furthermore, the results indicate that the invasion of C. pengoi induced a short-term increase in sexual reproduction in B. (E.) maritima. The results indicate that anthropogenic eutrophication since the 1950s has altered the zooplankton community toward smaller species, while the invasion of the predatory cladoceran had only a transitory influence on the community during its expansion phase.Peer reviewe

In vitro expansion of U87-MG human glioblastoma cells under hypoxic conditions affects glucose metabolism and subsequent in vivo growth

Hypoxia is a characteristic feature of solid tumors leading to the over expression of hypoxia-inducible factor (HIF)-1α protein and therefore to a specific cellular behavior. However, even though the oxygen tension in tumors is low (<5 %), most of the cell lines used in cancer studies are grown under 21 % oxygen tension. This work focuses on the impact of oxygen conditions during in vitro cell culture on glucose metabolism using 1-13C-glucose. Growing U87-MG glioma cells under hypoxic conditions leads to a two- to threefold reduction of labeled glutamine and an accumulation of fructose. However, under both hypoxic and normoxic conditions, glucose is used for de novo synthesis of pyrimidine since the 13C label is found both in the uracil and ribose moieties. Labeling of the ribose ring demonstrates that U87-MG glioma cells use the reversible branch of the non-oxidative pentose phosphate pathway. Interestingly, stereotactic implantation of U87-MG cells grown under normoxia or mild hypoxia within the striatum of nude mice led to differential growth; the cells grown under hypoxia retaining an imprint of the oxygen adaptation as their development is then slowed down

Aging Management and License Renewal

ABSTRACT Lifetime management of EDF PWR vessels and pipings are one of the main technical key points of safety and competitivness. This paper describes the EDF global approach in this field, which is applied to the nuclear fleet i.e 58 nuclear power plants, and particularly to the first 34 three loops, as far as lifetime is concerned : operating procedures and routine maintenance, special maintenance and ten years safety reassessment, engineering analysis ,based on feed back experience, scientific knowledge, degradations mechanisms, causes and consequences management, operating loadings decrease, complementary deterministic and cost-benefit analysis, fit for service justifications. anticipation strategy to prepare future, based on Non Destructive Testing investigations, ability to repair and/or to replace components, in situ expertises, ... Some examples are given : lifetime management of reactor vessels heads and bottom penetrations of pressure vessels, fit for service of cast stainless steel primary pipings, primary nozzles and auxiliary pipings special maintenance. 11