Reactors for microbial electrobiotechnology

From the first electromicrobial experiment to a sophisticated microbial electrochemical process - it all takes place in a reactor. Whereas the reactor design and materials used strongly influence the obtained results, there are no common platforms for MES reactors. This is a critical convention gap, as cross-comparison and benchmarking among MES as well as MES vs. conventional biotechnological processes is needed. Only knowledge driven engineering of MES reactors will pave the way to application and commercialization. In this chapter we first assess the requirements on reactors to be used for bioelectrochemical systems as well as potential losses caused by the reactor design. Subsequently, we compile the main types and designs of reactors used for MES so far, starting from simple H-cells to stirred tank reactors. We conclude with a discussion on the weaknesses and strengths of the existing types of reactors for bioelectrochemical systems that are scored on design criteria and draw conclusions for the future engineering of MES reactors. [GRAPHICS]

Thermodynamic properties of QCD in external magnetic fields

We consider the effect of strong external electromagnetic fields on thermodynamic observables in QCD, through lattice simulations with 1+1+1 flavors of staggered quarks at physical quark masses. Continuum extrapolated results are presented for the light quark condensates and for their tensor polarizations, as functions of the temperature and the magnetic field. We find the light condensates to undergo inverse magnetic catalysis in the transition region, in a manner that the transition temperature decreases with growing magnetic field. We also compare the results to other approaches and lattice simulations. Furthermore, we relate the tensor polarization to the spin part of the magnetic susceptibility of the QCD vacuum, and show that this contribution is diamagnetic.Comment: 13 pages, 9 figures, talks presented by FB and GE at Xth Quark Confinement and the Hadron Spectrum, 8-12 October 2012, TUM Campus Garching, Munich, German

Sacral Fractures and Associated Injuries.

STUDY DESIGN: Literature review. OBJECTIVE: The aim of this review is to describe the injuries associated with sacral fractures and to analyze their impact on patient outcome. METHODS: A comprehensive narrative review of the literature was performed to identify the injuries associated with sacral fractures. RESULTS: Sacral fractures are uncommon injuries that result from high-energy trauma, and that, due to their rarity, are frequently underdiagnosed and mistreated. Only 5% of sacral fractures occur in isolation. Injuries most often associated with sacral fractures include neurologic injuries (present in up to 50% of sacral fractures), pelvic ring disruptions, hip and lumbar spine fractures, active pelvic/ abdominal bleeding and the presence of an open fracture or significant soft tissue injury. Diagnosis of pelvic ring fractures and fractures extending to the lumbar spine are key factors for the appropriate management of sacral fractures. Importantly, associated systemic (cranial, thoracic, and abdominopelvic) or musculoskeletal injuries should be promptly assessed and addressed. These associated injuries often dictate the management and eventual outcome of sacral fractures and, therefore, any treatment algorithm should take them into consideration. CONCLUSIONS: Sacral fractures are complex in nature and often associated with other often-missed injuries. This review summarizes the most relevant associated injuries in sacral fractures and discusses on their appropriate management

The Development of a Universally Accepted Sacral Fracture Classification: A Survey of AOSpine and AOTrauma Members.

Study Design Survey study. Objective To determine the global perspective on controversial aspects of sacral fracture classifications. Methods While developing the AOSpine Sacral Injury Classification System, a survey was sent to all members of AOSpine and AOTrauma. The survey asked four yes-or-no questions to help determine the best way to handle controversial aspects of sacral fractures in future classifications. Chi-square tests were initially used to compare surgeons\u27 answers to the four key questions of the survey, and then the data was modeled through multivariable logistic regression analysis. Results A total of 474 surgeons answered all questions in the survey. Overall 86.9% of respondents felt that the proposed hierarchical nature of injuries was appropriate, and 77.8% of respondents agreed that that the risk of neurologic injury is highest in a vertical fracture through the foramen. Almost 80% of respondents felt that the separation of injuries based on the integrity of L5-S1 facet was appropriate, and 83.8% of surgeons agreed that a nondisplaced sacral U fracture is a clinically relevant entity. Conclusion This study determines the global perspective on controversial areas in the injury patterns of sacral fractures and demonstrates that the development of a comprehensive and universally accepted sacral classification is possible

The QCD phase diagram for external magnetic fields

The effect of an external (electro)magnetic field on the finite temperature transition of QCD is studied. We generate configurations at various values of the quantized magnetic flux with $N_f=2+1$ flavors of stout smeared staggered quarks, with physical masses. Thermodynamic observables including the chiral condensate and susceptibility, and the strange quark number susceptibility are measured as functions of the field strength. We perform the renormalization of the studied observables and extrapolate the results to the continuum limit using $N_t=6,8$ and 10 lattices. We also check for finite volume effects using various lattice volumes. We find from all of our observables that the transition temperature $T_c$ significantly decreases with increasing magnetic field. This is in conflict with various model calculations that predict an increasing $T_c(B)$. From a finite volume scaling analysis we find that the analytic crossover that is present at B=0 persists up to our largest magnetic fields $eB \approx 1 \textmd{GeV}^2$, and that the transition strength increases mildly up to this $eB\approx1 \textmd{GeV}^2$.Comment: 22 pages, 13 figure

Rescued Chondrogenesis of Mesenchymal Stem Cells under Interleukin 1 Challenge by Foamyviral Interleukin 1 Receptor Antagonist Gene Transfer

Background: Mesenchymal stem cells (MSCs) and their chondrogenic differentiation have been extensively investigated in vitro as MSCs provide an attractive source besides chondrocytes for cartilage repair therapies. Here we established prototype foamyviral vectors (FVV) that are derived from apathogenic parent viruses and are characterized by a broad host range and a favorable integration pattern into the cellular genome. As the inflammatory cytokine interleukin 1 beta (IL1β) is frequently present in diseased joints, the protective effects of FVV expressing the human interleukin 1 receptor antagonist protein (IL1RA) were studied in an established in vitro model (aggregate culture system) of chondrogenesis in the presence of IL1β.Materials and Methods: We generated different recombinant FVVs encoding enhanced green fluorescent protein (EGFP) or IL1RA and examined their transduction efficiencies and transgene expression profiles using different cell lines and human primary MSCs derived from bone marrow-aspirates. Transgene expression was evaluated by fluorescence microscopy (EGFP), flow cytometry (EGFP), and ELISA (IL1RA). For evaluation of the functionality of the IL1RA transgene to block the inhibitory effects of IL1β on chondrogenesis of primary MSCs and an immortalized MSC cell line (TERT4 cells), the cells were maintained following transduction as aggregate cultures in standard chondrogenic media in the presence or absence of IL1β. After 3 weeks of culture, pellets were harvested and analyzed by histology and immunohistochemistry for chondrogenic phenotypes.Results: The different FVV efficiently transduced cell lines as well as primary MSCs, thereby reaching high transgene expression levels in 6-well plates with levels of around 100 ng/ml IL1RA. MSC aggregate cultures which were maintained in chondrogenic media without IL1β supplementation revealed a chondrogenic phenotype by means of strong positive staining for collagen type II and matrix proteoglycan (Alcian blue). Addition of IL1β was inhibitory to chondrogenesis in untreated control pellets. In contrast, foamyviral mediated IL1RA expression rescued the chondrogenesis in pellets cultured in the presence of IL1β. Transduced MSC pellets reached thereby very high IL1RA transgene expression levels with a peak of 1087 ng/ml after day 7, followed by a decrease to 194 ng/ml after day 21, while IL1RA concentrations of controls were permanently below 200 pg/ml.Conclusion: Our results indicate that FVV are capable of efficient gene transfer to MSCs, while reaching IL1RA transgene expression levels, that were able to efficiently block the impacts of IL1β in vitro. FVV merit further investigation as a means to study the potential as a gene transfer tool for MSC based therapies for cartilage repair

Algebraic K-theory over the infinite dihedral group: an algebraic approach

We prove that the Waldhausen nilpotent class group of an injective index 2 amalgamated free product is isomorphic to the Farrell-Bass nilpotent class group of a twisted polynomial extension. As an application, we show that the Farrell-Jones Conjecture in algebraic K-theory can be sharpened from the family of virtually cyclic subgroups to the family of finite-by-cyclic subgroups

Interferometric 12CO(J=2-1) image of the Nuclear Region of Seyfert 1 Galaxy NGC 1097

We have mapped the central region of the Seyfert 1 galaxy NGC 1097 in 12CO(J=2-1) with the Submillieter Array (SMA). The 12CO(J=2-1) map shows a central concentration and a surrounding ring, which coincide respectively with the Seyfert nucleus and a starburst ring. The line intensity peaks at the nucleus, whereas in a previously published 12CO(J=1-0) map the intensity peaks at the starburst ring. The molecular ring has an azimuthally averaged 12CO(J=2-1)/(J=1-0) intensity ratio (R21) of about unity, which is similar to those in nearby active star forming galaxies, suggesting that most of the molecular mass in the ring is involved in fueling the starburst. The molecular gas can last for only about 1.2\times10^8 years without further replenishment assuming a constant star formation rate and a perfect conversion of gas to stars. The velocity map shows that the central molecular gas is rotating with the molecular ring in the same direction, while its velocity gradient is much steeper than that of the ring. This velocity gradient of the central gas is similar to what is usually observed in some Seyfert 2 galaxies. To view the active nucleus directly in the optical, the central molecular gas structure can either be a low-inclined disk or torus but not too low to be less massive than the mass of the host galaxy itself, be a highly-inclined thin disk or clumpy and thick torus, or be an inner part of the galactic disk. The R21 value of ~1.9 of the central molecular gas component, which is significantly higher than the value found at the molecular gas ring, indicates that the activity of the Seyfert nucleus may have a significant influence on the conditions of the molecular gas in the central component.Comment: 22 pages, 4 figures, accepted by Ap