Physical activity habits of the ESPN pupils and its relation with health pointers

This study intends to characterize habits and preferences concerned with physical activity of the Secondary School Pedro Nunes students. Moreover, we intended to understand the relation between some aspects of physical activity (volume: number of hours/week) and practice type (formal and informal practice) and health indicators: body composition, aerobic and muscular ability. With this study we also wish to promote among the scholar field the discussion on the role of the school and the physical education in the development and maintenance of the physical aptitude of the young people and the adoption of healthful habits of life.FCT (Fundação para a Ciência e a Tecnologia), IDP (Instituto do Desporto de Portugal), AIESEP World Congres

Olfatory and chemical caracterization of the Essential Oil from Bidens graveolens Mart.

IFF 2017

Scents from Brazilian Cerrado: The essential oil from Calea teucriifolia (Gardner) Baker (Asteraceae).

Edition of Abstracts of the 48th International Symposium on Essential Oils, Pécs, Hungary, 2017. Abstracts. Babedio, 2017. ISEO 2017, 10 a 13 set. 2017. P-63

Characterization of volatile and odor-active compounds of the essential oil from Bidens graveolens Mart. (Asteraceae).

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Hydrogen and carbon isotope fractionation during degradation of chloromethane by methylotrophic bacteria

Chloromethane (CH3Cl) is a widely studied volatile halocarbon involved in the destruction of ozone in the stratosphere. Nevertheless, its global budget still remains debated. Stable isotope analysis is a powerful tool to constrain fluxes of chloromethane between various environmental compartments which involve a multiplicity of sources and sinks, and both biotic and abiotic processes. In this study, we measured hydrogen and carbon isotope fractionation of the remaining untransformed chloromethane following its degradation by methylotrophic bacterial strains Methylobacterium extorquens CM4 and Hyphomicrobium sp. MC1, which belong to different genera but both use the cmu pathway, the only pathway for bacterial degradation of chloromethane characterized so far. Hydrogen isotope fractionation for degradation of chloromethane was determined for the first time, and yielded enrichment factors (epsilon) of -29 parts per thousand and -27 parts per thousand for strains CM4 and MC1, respectively. In agreement with previous studies, enrichment in C-13 of untransformed CH3Cl was also observed, and similar isotope enrichment factors (e) of -41 parts per thousand and -38 parts per thousand were obtained for degradation of chloromethane by strains CM4 and MC1, respectively. These combined hydrogen and carbon isotopic data for bacterial degradation of chloromethane will contribute to refine models of the global atmospheric budget of chloromethane

Draft genome sequences of gammaproteobacterial methanotrophs isolated from marine ecosystems

The genome sequences of Methylobacter marinus A45, Methylobacter sp. strain BBA5.1, and Methylomarinum vadi IT-4 were obtained. These aerobic methanotrophs are typical members of coastal and hydrothermal vent marine ecosystems

Phylogeny poorly predicts the utility of a challenging horizontally transferred gene in Methylobacterium strains

Horizontal gene transfer plays a crucial role in microbial evolution. While much is known about the mechanisms that determine whether physical DNA can be transferred into a new host, the factors determining the utility of the transferred genes are less clear. We have explored this issue using dichloromethane consumption in Methylobacterium strains. Methylobacterium extorquens DM4 expresses a dichloromethane dehalogenase (DcmA) that has been acquired through horizontal gene transfer and allows the strain to grow on dichloromethane as the sole carbon and energy source. We transferred the dcmA gene into six Methylobacterium strains that include both close and distant evolutionary relatives. The transconjugants varied in their ability to grow on dichloromethane, but their fitness on dichloromethane did not correlate with the phylogeny of the parental strains or with any single tested physiological factor. This work highlights an important limiting factor in horizontal gene transfer, namely, the capacity of the recipient strain to accommodate the stress and metabolic disruption resulting from the acquisition of a new enzyme or pathway. Understanding these limitations may help to rationalize historical examples of horizontal transfer and aid deliberate genetic transfers in biotechnology for metabolic engineering

Tetrachloromethane-degrading bacterial enrichment cultures and isolates from a contaminated aquifer

The prokaryotic community of a groundwater aquifer exposed to high concentrations of tetrachloromethane (CCl₄) for more than three decades was followed by terminal restriction fragment length polymorphism (T-RFLP) during pump-and-treat remediation at the contamination source. Bacterial enrichments and isolates were obtained under selective anoxic conditions, and degraded 10 mg·L(-1) CCl₄, with less than 10% transient formation of chloroform. Dichloromethane and chloromethane were not detected. Several tetrachloromethane-degrading strains were isolated from these enrichments, including bacteria from the Klebsiella and Clostridium genera closely related to previously described CCl₄ degrading bacteria, and strain TM1, assigned to the genus Pelosinus, for which this property was not yet described. Pelosinus sp. TM1, an oxygen-tolerant, Gram-positive bacterium with strictly anaerobic metabolism, excreted a thermostable metabolite into the culture medium that allowed extracellular CCl₄ transformation. As estimated by T-RFLP, phylotypes of CCl₄-degrading enrichment cultures represented less than 7%, and archaeal and Pelosinus strains less than 0.5% of the total prokaryotic groundwater community

Avaliação de leveduras industriais na fermentação de suco de caju.

A agroindustria do caju e, reconhecidamente, um dos mais importantes segmentos da economia do Nordeste brasileiro. representa parcela significativa das exportacoes da regiao, emprega cerca de 20 mil pessoas na industria processadora e gera em torno de 280 mil postos de trabalho no campo. O pedunculo de caju, alem de ser consumido in natura, possui varias opcoes tecnologicas de industrializacao, no entanto apenas o suco de caju integral possui alguma expressao economica. Por ser considerado um subproduto da agroindustria de amendoas, apresenta elevado indice de desperdicio, da ordem de 95% da producao anual, correspondendoem 1998, a 967.458 t (IBGE, 1998).bitstream/CNPAT-2010/5490/1/Ci-004.pd

Effective use of a horizontally-transferred pathway for dichloromethane catabolism requires post-transfer refinement

When microbes acquire new abilities through horizontal gene transfer, the genes and pathways must function under conditions with which they did not coevolve. If newly-acquired genes burden the host, effective use will depend on further evolutionary refinement of the recombinant strain. We used laboratory evolution to recapitulate this process of transfer and refinement, demonstrating that effective use of an introduced dichloromethane degradation pathway required one of several mutations to the bacterial host that are predicted to increase chloride efflux. We then used this knowledge to identify parallel, beneficial mutations that independently evolved in two natural dichloromethane-degrading strains. Finally, we constructed a synthetic mobile genetic element carrying both the degradation pathway and a chloride exporter, which preempted the adaptive process and directly enabled effective dichloromethane degradation across diverse Methylobacterium environmental isolates. Our results demonstrate the importance of post-transfer refinement in horizontal gene transfer, with potential applications in bioremediation and synthetic biology