The role of neutrophils in injury and repair following muscle stretch

Stretch injury to the myotendinous junction is a common problem in competitive athletes and those involved in regular physical activity. The major risk factor for recurrent injury appears to be the primary injury itself. Physicians, physical therapists, athletic trainers and athletes alike continue to search for optimal treatment and prevention strategies. Acute inflammation is regarded as the body's generalized protective response to tissue injury. An especially important and unexplored aspect of inflammation following injury is the role of inflammatory cells in extending injury and possibly directing muscle repair. It has been suggested that the inflammatory reaction, although it typically represents a reaction to damage and necrosis, may even bring about some local damage of its own and therefore increase the possibility for scarring and fibrosis. Limiting certain aspects of inflammation may theoretically reduce muscle damage as well as signals for muscle scarring. Here we focus on the role of neutrophils in injury and repair of stretch-injured skeletal muscle. A minimally invasive model that generates a reproducible injury to rabbit skeletal muscle is presented. We present a plausible theory that neutrophil-derived oxidants resulting from the initial stretch injury are responsible for extending the damage. An anti-CD11b antibody that blocks the neutrophil's respiratory burst is employed to reduce myofibre damage. An intriguing area that is currently being explored in our laboratory and others is the potential role for neutrophils to contribute to muscle growth and repair. It may be possible that neutrophils facilitate muscle repair through removal of tissue debris as well as by activation of satellite cells. Recent and ongoing investigations point to interleukin-6 as a possible key cytokine in muscle inflammation and repair. Studies to elucidate a clearer understanding of this possibility will be reviewed

Effects of eccentric phase velocity of plyometric training on the vertical jump

Abstract The aim of this study was to compare the effects of plyometric training performed with rapid or slow stretch contraction on jump performance and muscle properties. Thirty males between the ages of 19 and 22 volunteered for the 8-week experiment. Subjects were divided into the following three groups: training group 1 (TG1), training group 2 (TG2), and control group (CG). Each of the two experimental groups underwent a unique training regimen. For the first group (TG1, n = 12): from a standing position the subject flexed his knees to a 90° angle with velocity standardized and controlled at 0.4 m/s and immediately performed a leg extension as quickly as possible. For the second group (TG2, n = 12): from a standing position, the subject flexed his knees to a 90° angle with velocity standardized at 0.2 m/s and then performed a leg extension as quickly as possible. Each exercise consisted of six sets of ten repetitions with a barbell on the shoulders at 70 % of the maximal isometric force (1 RM). The 70 % load was modified at two-week intervals by evaluating a new 1 RM. Exercises were performed four times a week over the eight-week period. The third group (CG, n = 6), served as the control group. Maximal isometric force (MVC), maximal concentric force, squat jump (SJ) and counter movement jump (CMJ) exercises were performed before and after the training program. Subjects were filmed (100 Hz) and each jump was divided into three phases: eccentric phase (ECC), transition phase (TR) and concentric phase (CON). Surface EMG was used to determine the changes in the electromyographic (EMG) activity before and after the training program. There was an increase in leg extension force, velocity and electrical activity for SJ and CMJ for the two training groups (p < 0.05). However, TG1 showed a significant advantage in CMJ performance as well as a significant decrease in TR compared to the TG2 (p < 0.05). The results of this study show that when plyometric training is performed with rapid stretch contraction the CMJ jump height increases and the TR decreases