The Stellar Mass Density at z~6 from Spitzer Imaging of i-drop Galaxies

We measure the ages, stellar masses, and star formation histories of z~6 galaxies, observed within 1Gyr of the Big Bang. We use imaging from HST and Spitzer from the public "Great Observatories Origins Deep Survey", coupled with ground-based near-infrared imaging, to measure the spectral energy distributions from 0.8-5microns, spanning the rest-frame UV and optical. From our sample of ~50 i-drop Lyman-break star-forming galaxies in GOODS-South with z'(AB)<27mag, we focus on ~30 with reliable photometric or spectroscopic redshifts. Half of these are confused with foreground sources at Spitzer resolution, but from the 16 with clean photometry we find that a surprisingly large fraction (40%) have evidence for substantial Balmer-breaks. This indicates the presence of old underlying stellar populations that dominate the stellar masses. For these objects, we find ages of 200-700Myr, implying formation redshifts of 7<z<14, and large stellar masses in the range 1-3x10^10M_sun. Analysis of 7 i-drops that are undetected at 3.6microns indicates that these are younger, considerably less massive systems. We calculate that line contamination should not severely affect our photometry or derived results. Using data out to 8microns, we find little evidence for substantial intrinsic dust reddening. Correcting for incompleteness in our sample, we find a lower limit on the comoving stellar mass density at z~6 to be 2.5x10^6M_sun/Mpc^3. We are able to explore the star formation histories of our selected galaxies, and we infer that the past global star formation rate may have been much higher than that observed at z~6. The associated UV flux we infer at z>7 could have played a major role in reionizing the universe.Comment: Accepted for publication in MNRAS. Minor changes in response to referee repor

Spitzer Imaging of i'-drop Galaxies: Old Stars at z~6

We present new evidence for mature stellar populations with ages >100Myr in massive galaxies (M_stellar>10^10M_sun) seen at a time when the Universe was less than 1Gyr old. We analyse the prominent detections of two z~6 star-forming galaxies (SBM03#1 & #3) made at wavelengths corresponding to the rest-frame optical using the IRAC camera onboard the Spitzer Space Telescope. We had previously identified these galaxies in HST/ACS GOODS images of Chandra Deep Field South through the "i-drop" Lyman break technique, and subsequently confirmed spectroscopically with the Keck telescope. The new Spitzer photometry reveals significant Balmer/4000Ang discontinuities, indicative of dominant stellar populations with ages >100Myr. Fitting a range of population synthesis models (for normal initial mass functions) to the HST/Spitzer photometry yields ages of 250-650Myr and implied formation redshifts z~7.5-13.5 in presently-accepted world models. Remarkably, our sources have best-fit stellar masses of 1.3-3.8x10^10M_sun (95% confidence) assuming a Salpeter initial mass function. This indicates that at least some galaxies with stellar masses >20% of those of a present-day L* galaxy had already assembled within the first Gyr after the Big Bang. We also deduce that the past average star formation rate must be comparable to the current observed rate (SFR_UV~5-30M_sun/yr), suggesting that there may have been more vigorous episodes of star formation in such systems at higher redshifts. Although a small sample, limited primarily by Spitzer's detection efficiency, our result lends support to the hypothesis advocated in our earlier analyses of the Ultra Deep Field and GOODS HST/ACS data. The presence of established systems at z~6 suggests long-lived sources at earlier epochs (z>7) played a key role in reionizing the Universe.Comment: Accepted for publication in MNRAS (minor corrections made

Star Forming Galaxies at z~6 and Reionization

We determine the abundance of i'-band drop-outs in the HST/ACS GOODS surveys and the Hubble Ultra Deep Field (UDF). The majority of these sources are likely to be z~6 galaxies whose flux decrement arises from Lyman-alpha absorption. We have shown with Keck/DEIMOS spectroscopy that this technique does indeed select high redshift galaxies, and we discovered Lyman-alpha emission for about a third of the galaxies with z'_AB<25.6 The increased depth of UDF enables us to reach a ~10sigma limiting magnitude of z'_AB=28.5 (equivalent to 1.5M_sun/yr at z=6.1, or 0.1L*_UV for the z~3 U-drop population). The star formation rate at z~6 was approximately x6 LESS than at z~3. This declining comoving star formation rate poses an interesting challenge for models which suggest that L_UV>0.1L* star forming galaxies at z~6 reionized the universe. The short-fall in ionizing photons might be alleviated by galaxies fainter than our limit, or a radically different IMF. Alternatively, the bulk of reionization might have occurred at z>>6. We have recently discovered evidence of an early epoch of star formation in some of the i'-drops at z~6. Spitzer images with IRAC at 3.6-4.5microns show evidence of the age-sensitive Balmer/4000Ang break, dominated by stars older than 100Myr (and most probably 400Myr old). This pushes the formation epoch for these galaxies to z_form=7.5-13.5. There are at least some galaxies already assembled with stellar masses ~3x10^10M_sun (equivalent to 0.2M* today) within the first billion years. The early formation of such systems may have played a key role in reionizing the Universe at z~10.Comment: To appear in proceedings of UC Irvine May 2005 workshop on "First Light & Reionization", eds. E. Barton & A. Cooray, New Astronomy Reviews, in pres

The effects of 0.9% saline versus Plasma-Lyte 148 on renal function as assessed by creatinine concentration in patients undergoing major surgery: A single-centre double-blinded cluster crossover trial.

ObjectivesSaline and Plasma-Lyte have different physiochemical contents; consequently, they may differently affect patients' renal function. We compared the effects of fluid therapy with 0.9% saline and with Plasma-Lyte 148 on renal function as assessed by creatinine concentration among patients undergoing major surgery.MethodsWe conducted a prospective, double-blinded cluster crossover trial comparing the effects of the two fluids on major surgery patients. The primary aim was to establish the pilot feasibility, safety and preliminary efficacy evidence base for a large interventional trial to establish whether saline or Plasma-Lyte is the preferred crystalloid fluid for managing major surgery patients. The primary efficacy outcome was the proportion of patients with changes in renal function as assessed by creatinine concentration during their index hospital admission. We used changes in creatinine to define acute kidney injury (AKI) according to the RIFLE criteria.ResultsThe study was feasible with 100% patient and clinician acceptance. There were no deviations from the trial protocol. After screening, we allocated 602 patients to saline and 458 to Plasma-Lyte. The median (IQR) volume of intraoperative fluid received was 2000 mL (1000:2000) in both groups. Forty-nine saline patients (8.1%) and 49 Plasma-Lyte patients (10.7%) developed a postoperative AKI (adjusted incidence rate ratio [aIRR]: 1.34; 95% CI: 0.93-1.95; p = 0.120). No differences were observed in the development of postoperative complications (aIRR: 0.98; 95% CI: 0.89-1.08) or the severity of the worst complication (aIRR: 1.00; 95% CI: 0.78-1.30). The median (IQR) length of hospital stay was six days (3:11) for the saline group and five days (3:10) for the Plasma-Lyte group (aIRR: 0.85; 95% CI: 0.73-0.98). There were no serious adverse events relating to the trial fluids, nor were there fluid crossover or contamination events.ConclusionsThe study design was feasible to support a future follow-up larger clinical trial. Patients treated with saline did not demonstrate an increased incidence of postoperative AKI (defined as changes in creatinine) compared to those treated with Plasma-Lyte. Our findings imply that clinicians can reasonably use either solution intraoperatively for adult patients undergoing major surgery.Trial registrationRegistry: Australian New Zealand Clinical Trials Registry; ACTRN12613001042730; URL: https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=364988

Can we predict those with osteoarthritis who will worsen following a chronic disease management program?

Objective: To identify predictors of worsening symptoms and overall health of the treated hip or knee joint following 26 weeks of a nonsurgical chronic disease management program for hip and knee osteoarthritis (OA) and to examine the consistency of these predictors across 3 definitions of worsening. Methods: This prospective cohort study followed 539 participants of the program for 26 weeks. The 3 definitions of worsening included symptomatic worsening based on change in the Western Ontario and McMaster Universities Osteoarthritis Index Global score (WOMAC-G) measuring pain, stiffness, and function; a transition scale that asked about overall health of the treated hip or knee joint; and a composite outcome including both. Multivariate logistic regression models were constructed for the 3 definitions of worsening. Results: Complete data were available for 386 participants: mean age was 66.3 years, 69% were female, 85% reported knee joint pain as primary symptom (signal joint), 46% were waitlisted for total joint arthroplasty (TJA). TJA waitlist status, signal joint, 6-Minute Walk Test (6MWT), depressive symptoms, pain, and age were independently associated with at least 1 definition of worsening. TJA waitlist status and 6MWT remained in the multivariate models for the transition and composite definitions of worsening. Conclusion: Participants reporting worsening on the transition scale did not consistently meet the WOMAC-G definition of worsening symptoms. TJA waitlist status was predictive of the composite definition of worsening, a trend apparent for the transition definition. However, variables that predict worsening remain largely unknown. Further research is required to direct comprehensive and targeted management of patients with hip and knee OA.10 page(s

Skeletal muscle alterations and exercise performance decrease in erythropoietin-deficient mice: a comparative study

<p>Abstract</p> <p>Background</p> <p>Erythropoietin (EPO) is known to improve exercise performance by increasing oxygen blood transport and thus inducing a higher maximum oxygen uptake (VO_2max). Furthermore, treatment with (or overexpression of) EPO induces protective effects in several tissues, including the myocardium. However, it is not known whether EPO exerts this protective effect when present at physiological levels. Given that EPO receptors have been identified in skeletal muscle, we hypothesized that EPO may have a direct, protective effect on this tissue. Thus, the objectives of the present study were to confirm a decrease in exercise performance and highlight muscle transcriptome alterations in a murine EPO functional knock-out model (the EPO-d mouse).</p> <p>Methods</p> <p>We determined VO_2max peak velocity and critical speed in exhaustive runs in 17 mice (9 EPO-d animals and 8 inbred controls), using treadmill enclosed in a metabolic chamber. Mice were sacrificed 24h after a last exhaustive treadmill exercise at critical speed. The tibialis anterior and soleus muscles were removed and total RNA was extracted for microarray gene expression analysis.</p> <p>Results</p> <p>The EPO-d mice’s hematocrit was about 50% lower than that of controls (p < 0.05) and their performance level was about 25% lower (p < 0.001). A total of 1583 genes exhibited significant changes in their expression levels. However, 68 genes were strongly up-regulated (normalized ratio > 1.4) and 115 were strongly down-regulated (normalized ratio < 0.80). The transcriptome data mining analysis showed that the exercise in the EPO-d mice induced muscle hypoxia, oxidative stress and proteolysis associated with energy pathway disruptions in glycolysis and mitochondrial oxidative phosphorylation.</p> <p>Conclusions</p> <p>Our results showed that the lack of functional EPO induced a decrease in the aerobic exercise capacity. This decrease was correlated with the hematocrit and reflecting poor oxygen supply to the muscles. The observed alterations in the muscle transcriptome suggest that physiological concentrations of EPO exert both direct and indirect muscle-protecting effects during exercise. However, the signaling pathway involved in these protective effects remains to be described in detail.</p