110 research outputs found
Estudio y funcionalización quimioenzimática de los componentes químicos de Jasminum odoratissimum
De los extractos etanolicos de Jasminum odoratissimum se han aislado nueve secoiridoides glucosidicos: 10-hidoxioleosido dimetil ester, 10-acetoxioleosido dimetil ester, trans y cis-6¿-O-acetil-10-acetoxioleosido, trnas-10-(cinamoiloxi) oleosito dimetil ester y cis-10-(p-cuamroiloxi)oleosido dimetil ester. Todos ellos son identificados en base a sus datos espectroscopicos y son descritos por primera vez. Asimismo se obtienen dos nuevos iridoides 1a- y 1B, 7-dehidrologanetina. Se lleva a cabo la acetilacion parcialmente regioselectiva del anillo glucosidico del 10-acetoxi oleosido dimetilester,usando acetato como disolvente y reactivo y pancreatina Sigma como catalizador de la transesterificacion
Redes de monitoreo del agua subterránea en el Acuífero del Valle de Toluca. Propuesta de su distribución espacial mediante el manejo de factores hidrogeológicos, ambientales y socioeconómicos.
Contiene mapas, cuadros, imagenes rastrer y esquemasel presente trabajo justifica que una nueva propuesta para la distribución espacial de las redes de monitoreo del agua subterránea, permitirá obtener datos más confiables y actualizados de la cantidad y la calidad del recurso. Además, el resultado contribuirá a satisfacer los requerimientos del agua subterránea y los principales beneficiados serán los habitantes del área de estudio y los usuarios de este recurso, ya que podrán obtener el agua subterránea para sus diversas actividades (domésticas, agrícolas, e industriales).
En el contexto geográfico, el aporte consiste en entender el sistema hidrológico y su dinámica, analizar la influencia de los factores hidrogeológicos, ambientales y socioeconómicos, con una visión holística de la problemática, y así obtener información confiable y actualizada de los factores que influyen en la distribución espacial de las redes de monitoreo del agua subterránea. Es importante mencionar que desde el punto de vista científico, resulta conveniente que los pasos metodológicos puedan aplicarse a otros acuíferos con características semejantes
Antibiotic Resistance Changes in Gram-Positive Bacteria from Urine Cultures: Development Analysis in a Health Area of South-East Spain
This study analyzed the epidemiology and antibiotic susceptibility profile of significant
bacteriuria and assessed the impact of adopting EUCAST criteria on antibiotic resistances. A systematic
review was performed on publications in English or Spanish between 1 January 2010 and
30 June 2021 on the susceptibility of Gram-positive bacteria isolated in urinary samples in Europe.
A retrospective descriptive study was also conducted on the results of 21,838 urine cultures with
presumptive urinary tract infection (UTI) obtained during the past five years by the Department
of Microbiology of the Virgen de las Nieves University Hospital (Granada, Spain). The activity
of various antibiotics was determined, differentiated among various populations, and interpretations
compared according to the application of EUCAST or CLSI criteria. Among 21,838 cases
of significant bacteriuria, 27.69% were by Gram-positive bacteria, which were Enterococcus faecalis
in 19.04% and Enterococcus faecium in 3.92%. The susceptibility profile remained stable for most
antibiotics except for levofloxacin for E. faecalis and Staphylococcus aureus and nitrofurantoin for
E. faecium. The resistance of Enterococcus spp. and Staphylococcus spp. to glycopeptides was exceptionally
low in our setting. No significant difference in the prevalence ofmethicillin-resistant Staphylococcus
aureus was observed between hospital (26.67%) and community (28.85%) samples. Resistances in our
local setting remain stable and appear to be lower than reported in other studies. The adoption of
EUCAST vs. CLSI criteria did not produce a general change in resistance rates. Findings suggest
the need to revise certain empirical criteria, such as aminoglycoside synergy for Enterococcus and for
community-origin S. aureus
Rationale for the Use of Radiation-Activated Mesenchymal Stromal/Stem Cells in Acute Respiratory Distress Syndrome
We have previously shown that the combination of radiotherapy with human umbilical-cord-derived mesenchymal stromal/stem cells (MSCs) cell therapy significantly reduces the size of the xenotumors in mice, both in the directly irradiated tumor and in the distant nonirradiated tumor or its metastasis. We have also shown that exosomes secreted from MSCs preirradiated with 2 Gy are quantitatively, functionally and qualitatively different from the exosomes secreted from nonirradiated mesenchymal cells, and also that proteins, exosomes and microvesicles secreted by MSCs suffer a significant change when the cells are activated or nonactivated, with the amount of protein present in the exosomes of the preirradiated cells being 1.5 times greater compared to those from nonirradiated cells. This finding correlates with a dramatic increase in the antitumor activity of the radiotherapy when is combined with MSCs or with preirradiated mesenchymal stromal/stem cells (MSCs*). After the proteomic analysis of the load of the exosomes released from both irradiated and nonirradiated cells, we conclude that annexin A1 is the most important and significant difference between the exosomes released by the cells in either status. Knowing the role of annexin A1 in the control of hypoxia and inflammation that is characteristic of acute respiratory-distress syndrome (ARDS), we designed a hypothetical therapeutic strategy, based on the transplantation of mesenchymal stromal/stem cells stimulated with radiation, to alleviate the symptoms of patients who, due to pneumonia caused by SARS-CoV-2, require to be admitted to an intensive care unit for patients with life-threatening conditions. With this hypothesis, we seek to improve the patients’ respiratory capacity and increase the expectations of their cure.Ministerio de Economia y Competividad
MINECO: SAF2012-40011-C02-02
SAF2015-70520-R
RTICC RD12/0036/002
A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System
We describe a versatile, portable, and simple platform that includes a microfluidic electrochemical immunosensor for prostate-specific antigen (PSA) detection. It is based on the covalent immobilization of the anti-PSA monoclonal antibody on magnetic microbeads retained in the central channel of a microfluidic device. Image flow cytometry and scanning electron microscopy were used to characterize the magnetic microbeads. A direct sandwich immunoassay (with horseradish peroxidase-conjugated PSA antibody) served to quantify the cancer biomarker in serum samples. The enzymatic product was detected at -100 mV by amperometry on sputtered thin-film electrodes. Electrochemical reaction produced a current proportional to the PSA level, with a linear range from 10 pg mL(-1) to 1500 pg mL(-1). The sensitivity was demonstrated by a detection limit of 2 pg mL(-1) and the reproducibility by a coefficient of variation of 6.16%. The clinical performance of this platform was tested in serum samples from patients with prostate cancer (PCa), observing high specificity and full correlation with gold standard determinations. In conclusion, this analytical platform is a promising tool for measuring PSA levels in patients with PCa, offering a high sensitivity and reduced variability. The small platform size and low cost of this quantitative methodology support its suitability for the fast and sensitive analysis of PSA and other circulating biomarkers in patients. Further research is warranted to verify these findings and explore its potential application at all healthcare levels.Universidad Nacional de San Luis PROICO 22/Q241ANPCyT PICT 2018-04443
PICT-2015-2246
PICT-2015-1575
PICT-2014-1184
PICT-2014-0375
PICT-2018-04443Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) PIP 11220150100004COGENYOCentre for Genomics and Oncological Research: Pfizer-University of GranadaAndalusian Regional Government (Granada, Spain)ISCIII Health Research Institute P17/00989La Caixa FoundationHealth and Family Secretariat of the Andalusian Regional GovernmentSpanish GovernmentH2020-MSCA-IF-2019-89566
Breast cancer survival after 10 years of follow up, in Granada and Almería spanish provinces
Fundamento: Describir la supervivencia global y libre de enfermedad a los cinco y diez años del diagnóstico de cáncer de mama en mujeres participantes un estudio caso control previo, y establecer las variables pronóstico relacionadas.
Métodos: Se realizó el seguimiento de 202 mujeres diagnosticadas en tres hospitales públicos de Granada y Almería entre 1996-
1998. La supervivencia se evaluó mediante el método de Kaplan-Meier, y la identificación de factores relacionados mediante el análisis
de regresión de Cox.
Resultados: La edad media al diagnóstico fue de 54,27±10,4 años. La supervivencia global a los 5 años fue del 83,9% (IC 95%:
78,13-89,66) y a los 10 años del 71% (IC 95%: 63,25-78,74) con un tiempo medio de seguimiento de 119,91 meses (IC 95% 113,65-
126,17). La supervivencia libre de enfermedad a los 5 años fue del 81% (IC 95%: 74,52-87,47) y a los 10 años del 71,3% (IC 95%: 63,33-79,26) con un tiempo medio de seguimiento de 118,75 meses (IC 95% 111,86-125,65). La mortalidad de la serie fue del 33’17%.
Conclusiones: Las características de la enfermedad en las mujeres de la muestra estudiada son similares a las de otras regiones de España y Europa, con una supervivencia global superior a la descrita
en Europa y y comparable a la de España amba referidas para el mismo periodo.Background: To describe the overall and disease-free survival at five and ten years after breast cancer diagnosis in women from a previous case-control study, and establish related prognostic factors.
Methods:We followed up 202 patients diagnosed between 1996 and 1998 in three public hospitals in Granada and Almeria provinces in Spain. Survival rates were calculated using the Kaplan and Meier
method, and the Cox proportional hazards model was applied to identify the most significant variables contributing to survival.
Results: Mean age at diagnosis was 54.27±10.4 years. Mean follow-up for overall survival was 119.91 months (95%CI 113.65-
126.17); the five-year survival rate was 83.9% (95%CI: 78.13-89.66) and the ten-year rate was 71% (95%CI: 63.25-78.74). Mean followup for disease-free survival was 118.75 months (95%CI 111.86-125.65); the five-year disease-free survival rate was 81% (95%CI:74.52-87.47) and the ten-year rate was 71.3% (95%CI: 63.33-79.26).
The mortality rate of the study population was 33.17%.
Conclusions: Disease characteristics are similar in our population to those in other Spanish and European regions, while the overall survival is higher than the mean rate during the same period in Europe (5-yr rate of 79%) and similar to that in Spain (83%).Este trabajo ha sido financiado por proyectos de investigación de la Comisión Europea (7th. Frame Work, CONTAMED Nº: 212502) y de la Consejería de Salud de la Junta de Andalucía (SAS-0133/2007)
The efficacy of three-dimensional conformal radiation therapy on pain and quality of life in patients with painful bone metastases: a prospective study
AF, JE, and AM conceived and designed the study; all authors acquired the data; AF, JE, JP, and FM analyzed and interpreted the data; all authors drafted the manuscript; all authors critically revised the manuscript for important intellectual content; all authors gave approval of the version to be submitted; all authors agree to be accountable for all aspects of the work.Aim To evaluate the efficacy of radiation therapy in alleviating pain and improving the quality of life (QoL) with validated questionnaires in patients with painful bone metastases (BoM).
Methods This prospective, observational study recruited 167 patients with painful BoM who were treated with palliative radiotherapy (PRT) from February 2015 to February 2018. After the first clinical assessment, the patients filled out specific questionnaires and underwent a fast radiotherapy treatment within 48 hours. The patients were followed up for eight weeks.
Results The median age was 66.30 years. The most common primary cancer was lung cancer (31.1%). The most often prescribed scheme was 8 Gy in one fraction (70%). The patients experienced significant pain response and improved QoL compared with baseline, especially in the first two weeks after radiation. Overall, reduced pain and drug score were reported at two weeks of PRT in 68 (51.5%) and 37 (28%) of patients, respectively.
Conclusions PRT is an effective treatment option for patients with painful BoM.Fundación Progreso y Salud and fundación Publica Andaluza para la investigación Biosanitaria Andalucía Oriental (FIBAO
Cooperative and Escaping Mechanisms between Circulating Tumor Cells and Blood Constituents
Metastasis is the leading cause of cancer-related deaths and despite measurable progress
in the field, underlying mechanisms are still not fully understood. Circulating tumor cells (CTCs)
disseminate within the bloodstream, where most of them die due to the attack of the immune system.
On the other hand, recent evidence shows active interactions between CTCs and platelets, myeloid cells,
macrophages, neutrophils, and other hematopoietic cells that secrete immunosuppressive cytokines,
which aid CTCs to evade the immune system and enable metastasis. Platelets, for instance, regulate
inflammation, recruit neutrophils, and cause fibrin clots, which may protect CTCs from the attack
of Natural Killer cells or macrophages and facilitate extravasation. Recently, a correlation between the
commensal microbiota and the inflammatory/immune tone of the organism has been stablished. Thus,
the microbiota may affect the development of cancer-promoting conditions. Furthermore, CTCs may
suffer phenotypic changes, as those caused by the epithelial–mesenchymal transition, that also contribute
to the immune escape and resistance to immunotherapy. In this review,we discuss the findings regarding
the collaborative biological events among CTCs, immune cells, and microbiome associated to immune
escape and metastatic progression
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