Assimilation and removal of phosphorus within the marshland upwelling system

The marshland upwelling system (MUS) is an alternative onsite wastewater system that was developed to treat domestic wastewater derived from coastal communities. Previous studies have researched the systems ability to treat fecal coliforms and nitrogen. The objectives of this research were to (1) assess the treatment efficiency of the MUS for removing both total phosphorus and orthophosphate, (2) provide preliminary design and operational guidelines for phosphorus removal, and (3) develop a parameter to project longevity of the MUS for treating phosphorus. Performance efficiency was compared for a system which operated for 23 months located in Bayou Segnette State Park and that of a system which operated for 12 months in Moss Point, Mississippi. These two systems offered an investigation of MUS performance under low (Bayou Segnette MUS) and high (Moss Point MUS) background salinity conditions. An injection flowrate of 1.9 L/min at an injection frequency of 30 min/hr was used during the final study of the Bayou Segnette MUS. Total phosphorus surface concentrations were estimated to be 0.67 mg-P/L at the conclusion of this study. Research of the Moss Point system concluded with a flow regime of 2.8 L/min with an injection frequency of 15 min/hr. At the conclusion of this study, the predicted surface concentration for total phosphorus was 0.87 mg-P/L. Batch adsorption laboratory studies were conducted to develop isotherm coefficients for each of the subsurface soils found in these two systems. These studies were also conducted to investigate the impact which salinity would have on phosphate adsorption within the MUS. Freundlich coefficients ranged from 15.2 to 92.8 mg P/kg soil and were shown to be dependent on soil and salinity of the sample. Phosphate adsorption was shown to be slightly enhanced with and increase in salinity. However, the capacity of phosphate adsorption seemed to mostly dependent on the amount of Al, Ca and Fe contained within the soil of the MUS

Thermal Modification of Color in Red Alder Veneer. I. Effects of Temperature, Heating Time, and Wood Type

Red alder has become one of the most widely traded hardwood species in North America, and sliced red alder veneer is commonly applied as a decorative overlay on composite wood panels used by the furniture and cabinet industries. Red alder wood, however, acquires a mottled orange color following felling, which is undesirable when the wood is used for decorative purposes. Heating red alder wood remedies this problem to some extent, but there is still an unacceptable level of variability in the color of veneer sliced from heated veneer cants. This study examined the variation in color of red alder wood samples cut sequentially from the pith to the bark and subjected to heating under isothermal conditions. The aim was to examine whether within-tree variation in the susceptibility of red alder wood to thermal darkening can explain variation in color of veneer sliced from steamed red alder cants, and to determine the optimal thermal treatment (temperature and time) that can impart the tan color to red alder wood that industry is seeking. Results indicated that there was within-tree variation in the color of red alder samples following thermal treatment, but differences were pronounced only when wood was heated at a low temperature. Wood close to the bark tended to be redder than wood close to the pith when heated at 30°C, but such a difference was absent in wood heated at higher temperatures (50-90°C). Heating red alder wood, in vitro, at 70°C for 36 h produced wood that was evenly colored from pith to bark and matched the current industry color preference. It is suggested that the color of thermally modified red alder wood depends on the strength of reactions that produce orange/red chromophores in the wood, thermal darkening of the wood, and destruction of orange/red chromophores

Thermal Modification of Color in Red Alder Veneer. Part II. Effects of Season, Log Storage Time, and Location of Wood in Stems

The value of red alder lumber is diminished by discoloration caused by the enzyme-mediated polymerization of the diarylheptanoid xyloside, Oregonin that results in the formation of red-colored chromophores in freshly felled wood. This discoloration can be reduced by pre-steaming wood prior to kiln drying of lumber or veneer slicing, but in practice, there is still variation in the color of heat-treated wood, particularly in veneer sliced from heat-treated cants processed at different times of the year. There is seasonal variation in the concentration of Oregonin that is involved in the discoloration of red alder wood and it is hypothesized here that heat-treated red alder wood will be redder and darker when the wood is obtained from logs harvested during spring when the concentration of Oregonin is known to be higher than in other seasons. The aim of this research was to test this hypothesis, and also examine the effects of log storage time and location of wood in stems on the color of heat-treated red alder wood. The color of red alder wood subjected to an isothermal heat treatment at 70°C was strongly influenced by the season in which parent trees were harvested and the length of time that logs were stored prior to heat treatment of wood. In particular, wood harvested in spring and stored for 2 wk prior to heat treatment was significantly darker than similarly treated wood obtained from logs harvested in other seasons, and redder than wood harvested in summer and winter. If the storage time of logs harvested in spring and summer was extended to 4 wk, however, the heat-treated wood became lighter and less red. Heat-treated wood from the inner part of the logs was redder and darker than heat-treated wood from the outer part of the logs except occasionally, when the outer sapwood was obtained from logs harvested in spring or summer. Careful control of log storage time, heating temperature, and duration of heat treatment could be used to minimize seasonal variation in the color of veneer sliced from heated red alder cants

Comparison of Terrain Indices and Landform Classification Procedures in Low-Relief Agricultural Fields

Landforms control the spatial distribution of numerous factors associated with agronomy and water quality. Although curvature and slope are the fundamental surface derivatives used in landform classification procedures, methodologies for landform classifications have been performed with other terrain indices including the topographic position index (TPI) and the convergence index (CI). The objectives of this study are to compare plan curvature, the convergence index, profile curvature, and the topographic position index at various scales to determine which better identifies the spatial variability of soil phosphorus (P) within three low relief agricultural fields in central Illinois and to compare how two methods of landform classification, e.g. Pennock et al. (1987) and a modified approach to the TPI method (Weiss 2001, Jenness 2006), capture the variability of spatial soil P within an agricultural field. Soil sampling was performed on a 0.4 ha grid within three agricultural fields located near Decatur, IL and samples were analyzed for Mehlich-3 phosphorus. A 10-m DEM of the three fields was also generated from a survey performed with a real time kinematic global positioning system. The DEM was used to generate rasters of profile curvature, plan curvature, topographic position index, and convergence index in each of the three fields at scales ranging from 10 m to 150 m radii. In two of the three study sites, the TPI (r ≥ -0.42) was better correlated to soil P than profile curvature (r ≤ 0.41), while the CI (r ≥ -0.52) was better correlated to soil P than plan curvature (r ≥ -0.45) in all three sites. Although the Pennock method of landform classification failed to identify footslopes and shoulders, which are clearly part of these fields’ topographic framework, the Pennock method (R² = 0.29) and TPI method (R² = 0.30) classified landforms that captured similar amounts of soil P spatial variability in two of the three study sites. The TPI and CI should be further explored when performing terrain analysis at the agricultural field scale to create solutions for precision management objectives

Climate oscillations, glacial refugia, and dispersal ability: factors influencing the genetic structure of the least salmonfly, Pteronarcella badia (Plecoptera), in Western North America

Background: Phylogeographic studies of aquatic insects provide valuable insights into mechanisms that shape the genetic structure of communities, yet studies that include broad geographic areas are uncommon for this group. We conducted a broad scale phylogeographic analysis of the least salmonfly Pteronarcella badia (Plecoptera) across western North America. We tested hypotheses related to mode of dispersal and the influence of historic climate oscillations on population genetic structure. In order to generate a larger mitochondrial data set, we used 454 sequencing to reconstruct the complete mitochondrial genome in the early stages of the project. Results: Our analysis revealed high levels of population structure with several deeply divergent clades present across the sample area. Evidence from five mitochondrial genes and one nuclear locus identified a potentially cryptic lineage in the Pacific Northwest. Gene flow estimates and geographic clade distributions suggest that overland flight during the winged adult stage is an important dispersal mechanism for this taxon. We found evidence of multiple glacial refugia across the species distribution and signs of secondary contact within and among major clades. Conclusions: This study provides a basis for future studies of aquatic insect phylogeography at the inter-basin scale in western North America. Our findings add to an understanding of the role of historical climate isolations in shaping assemblages of aquatic insects in this region. We identified several geographic areas that may have historical importance for other aquatic organisms with similar distributions and dispersal strategies as P. badia. This work adds to the ever-growing list of studies that highlight the potential of next-generation DNA sequencing in a phylogenetic context to improve molecular data sets from understudied groups

Development and application of a novel and compact long-wavelength fluorescence spectrometer

Long wavelength (>600nm) fluorescence offers many advantages when applied to analysis, including minimal autofluorescence and scattered light from biological samples and the possibility of compact, robust, yet sensitive instruments. Modern clinical analysis has a number of specific requirements, namely specificity, sensitivity and speed, whilst the ability to monitor samples away from the laboratory is increasingly in demand. Immunoassays possess all these attributes and can be used in the following: environmental monitoring; clinical analysis; therapeutic drug monitoring. This research work describes a novel portable fluorescence spectrophotometer using long wavelength detection which can be used in all environments

A new long-wavelength fluorigenic substrate for alkaline phosphatase: synthesis and characterisation

Naphthofluorescein diphosphate has been synthesised from the parent dye, and shown to be an attractive longwavelength alternative to other fluorigenic substrates for the determination of alkaline phosphatase. Its application to the determination of theophylline, an inhibitor of this enzyme, has been demonstrated. The optimum excitation wavelength of the hydrolysis product naphthofluorescein has been found to depend on the presence of additives such as cyclodextrins and (3-[3-cholamidopropyl]-dimethylamino)-1-propane sulfonate (CHAPS): such effects can be used to raise the excitation wavelength to match the output of a 635 nm diode laser in a simple and sensitive fluorescence detector