Effects of osmotic and thermal shock on the invasive aquatic mudsnail Potamopyrgus antipodarum: Mortality and physiology under stressful conditions

Invasive freshwater species, such as the exotic mollusc Potamopyrgus antipodarum (New Zealand mudsnail), can frequently survive under harsh conditions, including brackish and hypoxic environments. We experimentally assessed the effects of osmotic (0, 10, 20, 25 and 30 psu) and thermal (20 °C) shock on mortality, activity and physiology of P. antipodarum collected at Capitol Lake, Olympia, Washington, USA, during winter and spring seasons when environmental temperature was 5 and 10 °C respectively. We measured standard metabolic rate and enzymatic activities (malate dehydrogenase, lactate dehydrogenase, alanopine dehydrogenase) in snails after a 10-day acclimation period at high salinity. Significantly higher mortalities were observed at higher salinities; the strongest effects occurred on snails collected at the end of winter, and exposed to 30 psu and 20 °C (100% mortality in 3 days). When snails were collected during the spring, 100% mortality was observed after 40 days at 30 psu and 20 °C. Standard metabolic rates were significantly lower when snails were exposed to salinities of 25 and 30 psu, even after 10 days of acclimation. Enzymatic activities showed small but significant declines after 10 days at 30 psu reflecting the declines observed in overall metabolism. The physiological tolerances to temperature and salinity displayed by this population of P. antipodarum make its eradication from Capital Lake difficult to achieve.Fil: Paolucci, Esteban Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Thuesen, Erik V.. The Evergreen State College; Estados Unido

Ventajas y límites de la variabilidad morfofisiológica para el establecimiento, la colonización y la expansión del bivalvo invasor Limnoperna fortunei en Sudamérica

La variabilidad fenotípica, tanto basada en plasticidad fenotípica como en diversidad genética, puede tener una gran importancia durante el proceso de invasión de las especies exóticas. Limnoperna fortunei (Dunker, 1857) fue introducido en Sudamérica alrededor de 1990, en el estuario del Río de la Plata, Argentina, desde donde se expandió rápidamente hasta abarcar prácticamente toda esta cuenca y otras aledañas. Sin embargo, algunos ambientes no han sido colonizados y, presumiblemente, la supervivencia de esta especie se vio afectada por distintos factores ambientales como los sólidos en suspensión, la salinidad, y la contaminación. L. fortunei evidenció una alta variabilidad genética y morfológica, mostrando una distribución fuertemente afectada por las actividades humanas y por sus vectores de dispersión. El grado de variabilidad fenotípica estaría jugando un papel fundamental en su establecimiento, o no, en los diferentes ambientes. Las variaciones morfológicas y metabólicas, incluyendo cambios en la tasa de crecimiento, desarrollo de órganos internos o cambios en la tasa de filtrado y respiración en áreas límite de su distribución o bajo condiciones ambientales extremas, permitirían explicar, junto con otras variables como la presencia de vectores de dispersión, el patrón de distribución actual

Benefícios e limites da variabilidade morfofisiológica para o estabelecimento, colonização e expansão do bivalve invasor Limnoperna fortunei na América do Sul

Phenotypic variability, both based on phenotypic plasticity and genetic diversity, can be a main factor affecting the invasion process of alien species. Limnoperna fortunei (Dunker 1857) was introduced in South America around 1990, in the Río de la Plata estuary, Argentina, from where it spread swiftly to cover most of the basin and other smaller watersheds. However, some environments have not been colonized and, presumably, the survival of this species was affected by different environmental factors such as suspended solids, salinity, and pollution. L. fortunei showed high genetic and morphological variability, showing a distribution strongly affected by human activities and its dispersal vectors. The degree of phenotypic variability would be playing a main role in its establishment, or not, in the different environments. Morphological and metabolic variations, including changes in the growth rate, development of internal organs or changes in the rate of filtration and respiration in border areas of its distribution or under extreme environmental conditions, would allow to explain, together with other variables such as the presence of dispersal vectors, the current distribution pattern.La variabilidad fenotípica, tanto basada en plasticidad fenotípica como en diversidad genética puede tener una gran importancia durante el proceso de invasión de las especies exóticas. Limnoperna fortunei (Dunker, 1857) fue introducido en Sudamérica alrededor de 1990, en el estuario del Río de la Plata, Argentina, desde donde se expandió rápidamente hasta abarcar prácticamente toda esta cuenca y otras aledañas. Sin embargo, algunos ambientes no han sido colonizados y, presumiblemente, la supervivencia de esta especie se vio afectada por distintos factores ambientales como los sólidos en suspensión, la salinidad, y la contaminación. L. fortunei evidenció una alta variabilidad genética y morfológica, mostrando una distribución fuertemente afectada por las actividades humanas y por sus vectores de dispersión. El grado de variabilidad fenotípica estaría jugando un papel fundamental en su establecimiento, o no, en los diferentes ambientes. Las variaciones morfológicas y metabólicas, incluyendo cambios en la tasa de crecimiento, desarrollo de órganos internos o cambios en la tasa de filtrado y respiración en áreas límite de su distribución o bajo condiciones ambientales extremas, permitirían explicar, junto con otras variables como la presencia de vectores de dispersión, el patrón de distribución actual.A variabilidade fenotípica, tanto baseada na plasticidade fenotípica quanto na diversidade genética, pode ser um dos principais fatores que afetam o processo de invasão de espécies exóticas. Limnoperna fortunei (Dunker, 1857) foi introduzido na América do Sul por volta de 1990, no estuário do Río de la Plata, Argentina, de onde se espalhou rapidamente para cobrir a maior parte da bacia e outras bacias hidrográficas menores. No entanto, alguns ambientes não foram colonizados e, presumivelmente, a sobrevivência desta espécie foi afetada por diferentes fatores ambientais, como sólidos em suspensão, salinidade e poluição. L. fortunei apresentou alta variabilidade genética e morfológica, apresentando uma distribuição fortemente afetada pelas atividades humanas e seus vetores de dispersão. O grau de variabilidade fenotípica estaria desempenhando um papel fundamental no seu estabelecimento, ou não, nos diferentes ambientes. Variações morfológicas e metabólicas, incluindo alterações na taxa de crescimento, desenvolvimento dos órgãos internos ou alterações na taxa de filtração e respiração em áreas fronteiriças de sua distribuição ou sob condições ambientais extremas, permitiriam explicar, em conjunto com outras variáveis ​​como a presença de vetores de dispersão, o padrão de distribuição atual

Combining ballast water treatment and ballast water exchange: Reducing colonization pressure and propagule pressure of phytoplankton organisms

Species richness and species abundance (colonization pressure and propagule pressure, respectively) are commonly used to characterize invasion risk for ballast-water-mediated introductions, which can be high if either parameter is high. For practical reasons, the adopted IMO-D2 standard for organisms in discharged ballast water only considers total abundance of biological indicators, without consideration of species richness or source community. Here we explore the effect of ballast-water source, ballast water exchange, chlorination, or a combination of both (hybrid treatment) on both colonization pressure and propagule pressure for one IMO-D2 size class (≥10 µm and \u3c50 \u3eµm; phytoplankton). A strong reduction of propagule pressure was observed in all experimental trials and taxonomic groups, probably owing to environment conditions inside ballast tanks and treatment effects. However, only the hybrid treatment met the IMO-D2 standard for propagule pressure, while also significantly reducing colonization pressure, from 25 initial groups to 16 final groups. In this treatment, dinoflagellates and diatoms dominated final composition. The impact of different treatments on colonization pressure and propagule pressure was always lower when the vessel was ballasted in a brackish than freshwater port. Our study demonstrates that even treated ballast water compliant with the IMO-D2 standard may still harbor a diverse phytoplankton community, albeit with low individual species abundances. These results might be similar even using a type approved ballast water management systems which usually includes a filter for \u3e50 µm organisms as a pre-treatment

First experimental demonstration of ABNO-driven in-operation flexgrid network re-optimization

The after failure repair optimization problem is used to experimentally validate in-operation planning using the ABNO architecture to control a flexgrid optical network. A back-end PCE is used to solve this computationally-intensive planning problem.Postprint (published version

First experimental assessment of ABNO-driven in-operation flexgrid network re-optimization

Traffic affected by link failures can be recovered using path restoration schemes. In dynamically operated networks provided with a control plane, restoration algorithms run in a centralized element, such as the path computation element (PCE). To increase traffic restorability in flexgrid networks, multiple paths, subconnections, can be used to restore every single affected connection. However, the multipath restoration scheme might result in a poor resource utilization entailing a lesser grade of service. In-operation network planning algorithms can be used to mitigate this problem once the failed link is repaired; we propose solving the so-called multipath after failure repair optimization problem (MP-AFRO) to reduce subconnections count by aggregating those belonging to the same original connection and rerouting the resulting connection to release spectral resources. The MP-AFRO problem is modeled using a mixed integer linear program formulation. In view of the complexity of the model and the limited time to solve the problem, we propose a heuristic algorithm that provides a good tradeoff between complexity and optimality. The performance on the MP-AFRO heuristic is firstly validated by simulation. Next, the heuristic algorithm is deployed inside an in-operation planning tool in the form of back-end PCE (bPCE) inside the application-based network operations architecture controlling a network; the bPCE is connected to the centralized active stateful PCE. MP-AFRO is experimentally demonstrated using a distributed field trial test-bed connecting the premises of Telefonica (Madrid), CNIT (Pisa), and UPC (Barcelona).Peer ReviewedPostprint (author’s final draft

Dynamic core VNT adaptability based on predictive metro-flow traffic models

© 2017 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes,creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.MPLS-over-optical virtual network topologies (VNTs) can be adapted to near-future traffic matrices based on predictive models that are estimated by applying data analytics on monitored origin-destination (OD) traffic. However, the deployment of independent SDN controllers for core and metro segments can bring large inefficiencies to this core network reconfiguration based on traffic prediction when traffic flows from metro areas are rerouted to different ingress nodes in the core. In such cases, OD traffic patterns in the core might severely change, thus affecting the quality of the predictive OD models. New traffic model re-estimation usually takes a long time, during which no predictive capabilities are available for the network operator. To alleviate this problem, we propose to extend data analytics to metro networks to obtain predictive models for the metro flows; by knowing how these flows are aggregated into OD pairs in the core, we can also aggregate their predictive models, thus accurately predicting OD traffic and therefore enabling core VNT reconfiguration. To obtain quality metro-flow models, we propose an estimation algorithmthat processes monitored data and returns a predictive model. In addition, a flow controller is proposed for the control architecture to allow metro and core controllers to exchange metro-flow model information. The proposed model aggregation is evaluated through exhaustive simulation, and eventually experimentally assessed together with the flow controller in a testbed connecting premises in CNIT (Pisa, Italy) and UPC (Barcelona, Spain).Peer ReviewedPostprint (author's final draft

Environment and shipping drive environmental DNA beta-diversity among commercial ports

The spread of nonindigenous species by shipping is a large and growing global problem that harms coastal ecosystems and economies and may blur coastal biogeographical patterns. This study coupled eukaryotic environmental DNA (eDNA) metabarcoding with dissimilarity regression to test the hypothesis that ship-borne species spread homogenizes port communities. We first collected and metabarcoded water samples from ports in Europe, Asia, Australia and the Americas. We then calculated community dissimilarities between port pairs and tested for effects of environmental dissimilarity, biogeographical region and four alternative measures of ship-borne species transport risk. We predicted that higher shipping between ports would decrease community dissimilarity, that the effect of shipping would be small compared to that of environment dissimilarity and shared biogeography, and that more complex shipping risk metrics (which account for ballast water and stepping-stone spread) would perform better. Consistent with our hypotheses, community dissimilarities increased significantly with environmental dissimilarity and, to a lesser extent, decreased with ship-borne species transport risks, particularly if the ports had similar environments and stepping-stone risks were considered. Unexpectedly, we found no clear effect of shared biogeography, and that risk metrics incorporating estimates of ballast discharge did not offer more explanatory power than simpler traffic-based risks. Overall, we found that shipping homogenizes eukaryotic communities between ports in predictable ways, which could inform improvements in invasive species policy and management. We demonstrated the usefulness of eDNA metabarcoding and dissimilarity regression for disentangling the drivers of large-scale biodiversity patterns. We conclude by outlining logistical considerations and recommendations for future studies using this approach.Fil: Andrés, Jose. Cornell University. Department Of Ecology And Evolutionary Biology;Fil: Czechowski, Paul. Cornell University. Department Of Ecology And Evolutionary Biology; . University of Otago; Nueva Zelanda. Helmholtz Institute for Metabolic, Obesity and Vascular Research; AlemaniaFil: Grey, Erin. University of Maine; Estados Unidos. Governors State University; Estados UnidosFil: Saebi, Mandana. University of Notre Dame; Estados UnidosFil: Andres, Kara. Cornell University. Department Of Ecology And Evolutionary Biology;Fil: Brown, Christopher. California State University Maritime Academy; Estados UnidosFil: Chawla, Nitesh. University of Notre Dame; Estados UnidosFil: Corbett, James J.. University of Delaware; Estados UnidosFil: Brys, Rein. Research Institute for Nature and Forest; BélgicaFil: Cassey, Phillip. University of Adelaide; AustraliaFil: Correa, Nancy. Ministerio de Defensa. Armada Argentina. Instituto Universitario Naval de la Ara. Escuela de Ciencias del Mar; Argentina. Ministerio de Defensa. Armada Argentina. Servicio de Hidrografía Naval; ArgentinaFil: Deveney, Marty R.. South Australian Research And Development Institute; AustraliaFil: Egan, Scott P.. Rice University; Estados UnidosFil: Fisher, Joshua P.. United States Fish and Wildlife Service; Estados UnidosFil: vanden Hooff, Rian. Oregon Department of Environmental Quality; Estados UnidosFil: Knapp, Charles R.. Daniel P. Haerther Center for Conservation and Research; Estados UnidosFil: Leong, Sandric Chee Yew. National University of Singapore; SingapurFil: Neilson, Brian J.. State of Hawaii Division of Aquatic Resources; Estados UnidosFil: Paolucci, Esteban Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Pfrender, Michael E.. University of Notre Dame; Estados UnidosFil: Pochardt, Meredith R.. M. Rose Consulting; Estados UnidosFil: Prowse, Thomas A. A.. University of Adelaide; AustraliaFil: Rumrill, Steven S.. Oregon Department of Fish and Wildlife; Estados UnidosFil: Scianni, Chris. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Instituto para el Estudio de la Biodiversidad de Invertebrados; Argentina. Marine Invasive Species Program; Estados UnidosFil: Sylvester, Francisco. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Instituto para el Estudio de la Biodiversidad de Invertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Tamburri, Mario N.. University of Maryland; Estados UnidosFil: Therriault, Thomas W.. Pacific Biological Station; CanadáFil: Yeo, Darren C. J.. National University of Singapore; SingapurFil: Lodge, David M.. Cornell University. Department Of Ecology And Evolutionary Biology

Biometric conversion factors as a unifying platform for comparative assessment of invasive freshwater bivalves

Invasive bivalves continue to spread and negatively impact freshwater ecosystems worldwide. As different metrics for body size and biomass are frequently used within the literature to standardise bivalve-related ecological impacts (e.g. respiration and filtration rates), the lack of broadly applicable conversion equations currently hinders reliable comparison across bivalve populations. To facilitate improved comparative assessment among studies originating from disparate geographical locations, we report body size and biomass conversion equations for six invasive freshwater bivalves (or species complex members) worldwide: Corbicula fluminea, C. largillierti, Dreissena bugensis, D. polymorpha, Limnoperna fortunei and Sinanodonta woodiana, and tested the reliability (i.e. precision and accuracy) of these equations. Body size (length, width and height) and biomass metrics of living-weight (LW), wet-weight (WW), dry-weight (DW), dry shell-weight (SW), shell free dry-weight (SFDW) and ash-free dry-weight (AFDW) were collected from a total of 44 bivalve populations located in Asia, the Americas and Europe. Relationships between body size and individual biomass metrics, as well as proportional weight-to-weight conversion factors, were determined. For most species, although inherent variation existed between sampled populations, body size directional measurements were found to be good predictors of all biomass metrics (e.g. length to LW, WW, SW or DW: R2 = 0.82–0.96), with moderate to high accuracy for mean absolute error (MAE): ±9.14%–24.19%. Similarly, narrow 95% confidence limits and low MAE were observed for most proportional biomass relationships, indicating high reliability for the calculated conversion factors (e.g. LW to AFDW; CI range: 0.7–2.0, MAE: ±0.7%–2.0%). Synthesis and applications. Our derived biomass prediction equations can be used to rapidly estimate the biologically active biomass of the assessed species, based on simpler biomass or body size measurements for a wide range of situations globally. This allows for the calculation of approximate average indicators that, when combined with density data, can be used to estimate biomass per geographical unit-area and contribute to quantification of population-level effects. These general equations will support meta-analyses, and allow for comparative assessment of historic and contemporary data. Overall, these equations will enable conservation managers to better understand and predict ecological impacts of these bivalves.Fil: Coughlan, Neil E.. The Queens University of Belfast; Irlanda. University College Cork; IrlandaFil: Cunningham, Eoghan M.. The Queens University of Belfast; IrlandaFil: Cuthbert, Ross N.. The Queens University of Belfast; Irlanda. Geomar-Helmholtz Centre for Ocean Research Kiel; AlemaniaFil: Joyce, Patrick W. S.. The Queens University of Belfast; IrlandaFil: Anastácio, Pedro. Universidade de Évora; PortugalFil: Banha, Filipe. Universidade de Évora; PortugalFil: Bonel, Nicolás. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Bradbeer, Stephanie J.. University of Leeds; Reino UnidoFil: Briski, Elizabeta. Geomar-Helmholtz Centre for Ocean Research Kiel; AlemaniaFil: Butitta, Vince L.. University of Wisconsin; Estados UnidosFil: Cadková, Zuzana. Czech University of Life Sciences; República ChecaFil: Dick, Jaimie T. A.. The Queens University of Belfast; IrlandaFil: Douda, Karel. Czech University of Life Sciences; República ChecaFil: Eagling, Lawrence E.. The Queens University of Belfast; IrlandaFil: Ferreira Rodríguez, Noé. Universidad de Vigo; EspañaFil: Hünicken, Leandro Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Johansson, Mattias L.. University of North Georgia; Estados UnidosFil: Kregting, Louise. The Queens University of Belfast; IrlandaFil: Labecka, Anna Maria. Jagiellonian University; PoloniaFil: Li, Deliang. Hunan Agricultural University; ChinaFil: Liquin, Florencia Fernanda. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Instituto para el Estudio de la Biodiversidad de Invertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Marescaux, Jonathan. University of Namur; Bélgica. e-biom; BélgicaFil: Morris, Todd J.. Fisheries and Ocean Canada; CanadáFil: Nowakowska, Patrycja. University of Gdansk; PoloniaFil: Ozgo, Malgorzata. Kazimierz Wielki University; PoloniaFil: Paolucci, Esteban Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Peribáñez, Miguel A.. Universidad de Zaragoza; EspañaFil: Riccardi, Nicoletta. Consiglio Nazionale delle Ricerche; ItaliaFil: Smith, Emily R. C.. University College London; Estados UnidosFil: Sylvester, Francisco. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Instituto para el Estudio de la Biodiversidad de Invertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentin

Characteristics of hepatitis C virus resistance in an international cohort after a decade of direct-acting antivirals

Background & Aims: Direct-acting antiviral (DAA) regimens provide a cure in >95% of patients with chronic HCV infection. However, in some patients in whom therapy fails, resistance-associated substitutions (RASs) can develop, limiting retreatment options and risking onward resistant virus transmission. In this study, we evaluated RAS prevalence and distribution, including novel NS5A RASs and clinical factors associated with RAS selection, among patients who experienced DAA treatment failure. Methods: SHARED is an international consortium of clinicians and scientists studying HCV drug resistance. HCV sequence linked metadata from 3,355 patients were collected from 22 countries. NS3, NS5A, and NS5B RASs in virologic failures, including novel NS5A substitutions, were examined. Associations of clinical and demographic characteristics with RAS selection were investigated. Results: The frequency of RASs increased from its natural prevalence following DAA exposure: 37% to 60% in NS3, 29% to 80% in NS5A, 15% to 22% in NS5B for sofosbuvir, and 24% to 37% in NS5B for dasabuvir. Among 730 virologic failures, most were treated with first-generation DAAs, 94% had drug resistance in ≥1 DAA class: 31% single-class resistance, 42% dual-class resistance (predominantly against protease and NS5A inhibitors), and 21% triple-class resistance. Distinct patterns containing ≥2 highly resistant RASs were common. New potential NS5A RASs and adaptive changes were identified in genotypes 1a, 3, and 4. Following DAA failure, RAS selection was more frequent in older people with cirrhosis and those infected with genotypes 1b and 4. Conclusions: Drug resistance in HCV is frequent after DAA treatment failure. Previously unrecognized substitutions continue to emerge and remain uncharacterized. Lay summary: Although direct-acting antiviral medications effectively cure hepatitis C in most patients, sometimes treatment selects for resistant viruses, causing antiviral drugs to be either ineffective or only partially effective. Multidrug resistance is common in patients for whom DAA treatment fails. Older patients and patients with advanced liver diseases are more likely to select drug-resistant viruses. Collective efforts from international communities and governments are needed to develop an optimal approach to managing drug resistance and preventing the transmission of resistant viruses