Interstrand DNA covalent binding of two dinuclear Ru(ii) complexes. Influence of the extra ring of the bridging ligand on the DNA interaction and cytotoxic activity

In this work, we report experimental and computational evidences for the intercalation into the DNA base pairs of the free quinones Quinizarin (Q), Naphthazain (N) and the interstrad covalent binding of their p-cymene di-Ruthenium(II) complexes (Cl2Ru2X, with X = N, Q bridging ligands). The intercalation extent for the N complex was larger than for Q, in good agreement with higher relative contour length and melting temperature for the same CX/CDNA ratio and with the computacional mean stacking distances between the ligand and the nearest base-pair (3.34Å and 3.19Å) for N and Q, respectively. However, the apparent binding constant of Q/DNA, two orders higher than that of N/DNA, denotes that the thermal stability of X/DNA complex is more related to the degree of intercalation than to the binding constants magnitude. Cl2Ru2X complexes undergo aquation, forming the aqua-derivatives [(H2O)2Ru2X]2+. These can further bind covalently to DNA via interstrand crosslinking, through both Ru centres and two N7 sites of consecutive Guanines, to give (DNA1,2)Ru2X complexes, by a mechanism similar to that of cisplatin. To the best of our knowledge, this type of interaction with dinuclear Ru(II) complexes has not been reported hitherto. The experimental and computational results reveal that the number of rings of the aromatic moiety and the covalent binding to DNA play a key role in the behaviour of the quinones and their Ru(II) derivatives. The cytotoxicity of the ligands and the corresponding Ru(II) complexes was evaluated in the MCF-7, A2780, A2780cis tumour cells and in the healthy cell line MRC-5. The cytotoxic activity was notable for the N compound and negligible for Q. The IC50 values and the resistance (RF) and selectivity (SF) factors show that the Cl2Ru2N complex is the most promising among the four studied anticancer drugs

Spectroscopy of proton-unbound nuclei by tracking their decay products in-flight: One- and two-proton decays of 15F, 16Ne, and 19Na

14 páginas, 23 figuras, 1 tabla.-- et al.A powerful method of investigating proton-unbound nuclear states by tracking their decay products in flight is discussed in detail. To verify the method, four known levels in 15F, 16Ne, and 19Na were investigated by measuring the angular correlations between protons and the respective heavy-ion fragments stemming from the precursor decays in flight. The parent nuclei of interest were produced in nuclear reactions of one-neutron removal from 17Ne and 20Mg projectiles at energies of 410–450 A MeV. The trajectories of the respective decay products, 14O + p + p and 18Ne + p + p, were measured by applying a tracking technique with microstrip detectors. These data were used to reconstruct the angular correlations of the fragments, which provided information on energies and widths of the parent states. In addition for reproducing properties of known states, evidence for hitherto unknown excited states in 15F and 16Ne was found. This tracking technique has an advantage in studies of exotic nuclei beyond the proton drip line measuring the resonance energies and widths with a high precision although by using low-intensity beams and very thick targets.This work has been supported by Contract EURONS Nos. EC-I3 and FPA2006-13807-C02-01, FPA2007-63074 (MEC, Spain), the INTAS Grant No. 03-54-6545. L.V.G. is supported by FAIR-Russia Research Center grant, Russian Foundation for Basic Research viaGrant Nos. RFBR 08-02-00892 and 08-02-00089-a, and Russian Ministry of Industry and Science Grant No. NSh-7235.2010.2. N.K.T. acknowledges support from the UK Grant No. STFC ST/F012012/1, E.L. is supported by the LOEWE program of the State of Hessen (Helmholtz International Center for FAIR), Germany.Peer Reviewe

Characterization of fruit and vegetable waste as an alternative ruminant feed in Pachuca, Hidalgo, Mexico

Objective: to characterize and measure the amount of fruit residues generated during four weeks in a commercial establishment dedicated to the elaboration and sale of fruit juices and cocktails in Pachuca City, Hidalgo, Mexico. Design/Methodology/Approximation: The total of fruit residues generated in a working day (one day) in an establishment dedicated to the sale of juices and smoothies in the city of Pachuca de Soto, Hidalgo, Mexico was collected from Monday to Saturday during four weeks in September and October 2019. The residues were daily weighed and separated according to the type of fruit and analyzed for dry matter, crude protein and ash. The design was completely random. Results: the orange peel predominated, and the volume of residues was constant accumulating 465 kg during the four weeks. The moisture content was from 75 to 94%, depending on the type of fruit. The CP concentration was from 4.33 to 6.95% except for the papaya peel (11.55% CP) Limitations/Implications: An alternative to avoid landfill deposition of this wastes is to subject it to a silage process and thus reduce negative impacts on the environment, generate valuable products and reduce pressure on natural resources. Findings/Conclusions: The amount of fruit and vegetable wastes is constant throughout the week, its proportion of dry matter is low, but it can be combined with cereal straw and ensiled to generate valuable products and avoid its deposition in landfills.Objective: Measure and characterize the fruit and vegetable waste generated duringa working day in a commercial juice and fruit cocktail establishment in Pachuca,Hidalgo, Mexico, for four weeks.Design/ methodology/ approach: The total amount of fruit waste generated duringa working day in a commercial juice and smoothie establishment in Pachuca,Hidalgo, Mexico, was collected Monday through Saturday for four weeks inSeptember and October 2019. Waste was weighted and separated daily into thedifferent types of fruit. We then analyzed their dry matter (DM), crude protein (CP),and ash (Ash) content. The experimental design was completely randomized.Results: The amount of fruit and vegetable waste was constant during the fourcollection weeks; the total amount of waste collected was 465 kg; orange peel wasthe most abundant waste (75%). The moisture content ranged from 75 to 94% basedon the type of fruit. The CP concentration ranged from 4.33 to 6.95%, except for thepapaya peel, which had a CP content of 11.55%.Limitations/ implications: An alternative to avoid landfilling this type of organicwaste is to subject it to a silage-making process; this would reduce negative 2environmental impacts, generate value-added products, and reduce the pressure onnatural resources.Findings/ conclusions: The amount of fruit and vegetable waste produced everyweek was constant for four weeks. Although their dry matter content is low, thesewastes, mixed with cereal straw and subjected to a silage-making process, can resultin value-added products, and avoid landfilling

Yield and oil content of castor oil plant (Ricinus communis L.) accessions grown with fertigation in a semiarid zone

Objective: To evaluate seed, dry matter, and oil content yield in five accessions of castor oil plant (Ricinus communis L.) in the Altiplano Potosino Zacatecano high plateau region of Mexico, grown with a fertigation system on a mixture of sand, tezontle, and compost, with three fertilization levels.Design/Methodology/Approach: The following accessions showed an outstandingly favorable response to fertilization: ZACS2C1 (Orito Zac) which had the greater seed yield (735 g) and oil content (50.30 ± 5.23); and SLPS11C1 which had the greater weight per100 seeds (56.77 ±2.35) and dry matter (1600 g).Results: The best fertilization level for the abovementioned accessions was obtained with the low nutrient solution,Limitations/Implications of the study: while other accessions showed a variable and inverse response to the fertilization level. Results/Conclusions: To make the most of the castor oil plant, oil or fodder accessions can be selected or both accessions can be sown under an intercropping system

Guía de gestión integrada de plagas: aguacate

Coordinadores: Ángel Martín Gil y Gregoria Aranda Aranda

Elastic scattering and α -particle production in 6 He + 208 Pb collisions at 22 MeV

Experimental results of the elastic scattering of 6He on 208Pb at E LAB=22 MeV, measured at the CRC facility (Louvain-la-Neuve, Belgium), are presented, including results on the 4He production channel. These data were taken with full angular coverage and high angular resolution. Both experimental cross sections are compared with continuum discretized coupled channels and distorted-wave Born approximation calculations, where direct breakup and transfer to the continuum processes are considered. The elastic data confirm the absence of the Coulomb rainbow, while the distribution of α particles indicates that such production is mostly generated by transfer to the continuum.MICINN FPA2005-04460 FPA200502379 FPA2006-13807-c02-01 FPA2007-63074 FPA2009-07653 FPA2009-07387 FPA2010-22131-C02-01Junta de Andalucía FQM-4964Programa Consolider-Ingenio 2010 CSD2007-00042Comisión Europea HPRI-CT-1999-0011

Prospective associations between a priori dietary patterns adherence and kidney function in an elderly Mediterranean population at high cardiovascular risk

Purpose To assess the association between three different a priori dietary patterns adherence (17-item energy reduced-Mediterranean Diet (MedDiet), Trichopoulou-MedDiet and Dietary Approach to Stop Hypertension (DASH)), as well as the Protein Diet Score and kidney function decline after one year of follow-up in elderly individuals with overweight/obesity and metabolic syndrome (MetS). Methods We prospectively analyzed 5675 participants (55-75 years) from the PREDIMED-Plus study. At baseline and at one year, we evaluated the creatinine-based estimated glomerular filtration rate (eGFR) and food-frequency questionnaires-derived dietary scores. Associations between four categories (decrease/maintenance and tertiles of increase) of each dietary pattern and changes in eGFR (ml/min/1.73m(2)) or >= 10% eGFR decline were assessed by fitting multivariable linear or logistic regression models, as appropriate. Results Participants in the highest tertile of increase in 17-item erMedDiet Score showed higher upward changes in eGFR (beta: 1.87 ml/min/1.73m(2); 95% CI: 1.00-2.73) and had lower odds of >= 10% eGFR decline (OR: 0.62; 95% CI: 0.47-0.82) compared to individuals in the decrease/maintenance category, while Trichopoulou-MedDiet and DASH Scores were not associated with any renal outcomes. Those in the highest tertile of increase in Protein Diet Score had greater downward changes in eGFR (beta: - 0.87 ml/min/1.73m(2); 95% CI: - 1.73 to - 0.01) and 32% higher odds of eGFR decline (OR: 1.32; 95% CI: 1.00-1.75). Conclusions Among elderly individuals with overweight/obesity and MetS, only higher upward change in the 17-item erMedDiet score adherence was associated with better kidney function after one year. However, increasing Protein Diet Score appeared to have an adverse impact on kidney health. Trial Registration Number: ISRCTN89898870 (Data of registration: 2014).Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by the official Spanish Institutions for funding scientific biomedical research, CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN) and Instituto de Salud Carlos III (ISCIII), through the Fondo de Investigacion para la Salud (FIS), which is co-funded by the European Regional Development Fund (six coordinated FIS projects leaded by JS-S and JVi, including the following projects: PI13/00673, PI13/00492, PI13/00272, PI13/01123, PI13/00462, PI13/00233, PI13/02184, PI13/00728, PI13/01090, PI13/01056, PI14/01722, PI14/00636, PI14/00618, PI14/00696, PI14/01206, PI14/01919, PI14/00853, PI14/01374, PI14/00972, PI14/00728, PI14/01471, PI16/00473, PI16/00662, PI16/01873, PI16/01094, PI16/00501, PI16/00533, PI16/00381, PI16/00366, PI16/01522, PI16/01120, PI17/00764, PI17/01183, PI17/00855, PI17/01347, PI17/00525, PI17/01827, PI17/00532, PI17/00215, PI17/01441, PI17/00508, PI17/01732, PI17/00926, PI19/00957, PI19/00386, PI19/00309, PI19/01032, PI19/00576, PI19/00017, PI19/01226, PI19/00781, PI19/01560, PI19/01332, PI20/01802, PI20/00138, PI20/01532, PI20/00456, PI20/00339, PI20/00557, PI20/00886, PI20/01158); the Especial Action Project entitled: Implementacion y evaluacion de una intervencion intensiva sobre la actividad fisica Cohorte PREDIMED-Plus grant to JS-S; the European Research Council (Advanced Research Grant 2014-2019; agreement #340918) granted to MAMG.; the Recercaixa (number 2013ACUP00194) grant to JS-S; grants from the Consejeria de Salud de la Junta de Andalucia (PI0458/2013, PS0358/2016, PI0137/2018); the PROMETEO/2017/017 and the PROMETEO 21/2021 grant from the Generalitat Valenciana; the SEMERGEN grant; the Boosting young talent call grant program for the development of IISPV research projects 2019-2021 (Ref.: 2019/IISPV/03 grant to AD-L); the Societat Catalana d'Endocrinologia i Nutricio (SCEN) Clinical-Research Grant 2019 (IPs: JS-S and AD-L). Collaborative Nutrition and/or Obesity Project for Young Researchers 2019 supported by CIBEROBN entitled: Lifestyle Interventions and Chronic Kidney Disease: Inflammation, Oxidative Stress and Metabolomic Profile (LIKIDI study) grant to AD-L. Jordi Salas-Salvado, gratefully acknowledges the financial support by ICREA under the ICREA Academia programme. M.R.-G., is supported by the Ministry of Education of Spain (FPU17/06488). None of the funding sources took part in the design, collection, analysis, interpretation of the data, or writing the report, or in the decision to submit the manuscript for publication

Proton-proton correlations observed in two-proton decay of (19)Mg and (16)Ne

Proton-proton correlations were observed for the two-proton decays of the ground states of 19Mg and 16Ne. The trajectories of the respective decay products, 17Ne+p+p and 14O+p+p, were mea- sured by using a tracking technique with microstrip detectors. These data were used to reconstruct the angular correlations of fragments projected on planes transverse to the precursor momenta. The measured three-particle correlations reflect a genuine three-body decay mechanism and allowed us to obtain spectroscopic information on the precursors with valence protons in the sd shel

Guía de gestión integrada de plagas: mango

Coordinadores: Ángel Martín Gil y Gregoria Aranda Aranda

Dietary diversity and depression: cross-sectional and longitudinal analyses in Spanish adult population with metabolic syndrome. Findings from PREDIMED-Plus trial

Objective: To examine the cross-sectional and longitudinal (2-year follow-up) associations between dietary diversity (DD) and depressive symptoms. Design: An energy-adjusted dietary diversity score (DDS) was assessed using a validated FFQ and was categorised into quartiles (Q). The variety in each food group was classified into four categories of diversity (C). Depressive symptoms were assessed with Beck Depression Inventory-II (Beck II) questionnaire and depression cases defined as physician-diagnosed or Beck II >= 18. Linear and logistic regression models were used. Setting: Spanish older adults with metabolic syndrome (MetS). Participants: A total of 6625 adults aged 55–75 years from the PREDIMED-Plus study with overweight or obesity and MetS. Results: Total DDS was inversely and statistically significantly associated with depression in the cross-sectional analysis conducted; OR Q4 v. Q1 = 0·76 (95 % CI (0·64, 0·90)). This was driven by high diversity compared to low diversity (C3 v. C1) of vegetables (OR = 0·75, 95 % CI (0·57, 0·93)), cereals (OR = 0·72 (95 % CI (0·56, 0·94)) and proteins (OR = 0·27, 95 % CI (0·11, 0·62)). In the longitudinal analysis, there was no significant association between the baseline DDS and changes in depressive symptoms after 2 years of follow-up, except for DD in vegetables C4 v. C1 = (β = 0·70, 95 % CI (0·05, 1·35)). Conclusions: According to our results, DD is inversely associated with depressive symptoms, but eating more diverse does not seem to reduce the risk of future depression. Additional longitudinal studies (with longer follow-up) are needed to confirm these findings.T The PREDIMED-Plus trial was supported by the European Research Council (Advanced Research Grant 2013-2018; 340918) grant to Miguel Angel Martinez-Gonzalez, and by the official funding agency for biomedical research of the Spanish Government, ISCIII through the Fondo de Investigacion para la Salud (FIS), which is cofunded by the European Regional Development Fund (four coordinated FIS projects led by Jordi Salas-Salvado and Josep Vidal), including the following projects: PI13/00673, PI13/00492, PI13/00272, PI13/01123, PI13/00462, PI13/00233, PI13/02184, PI13/00728, PI13/01090, PI13/01056, PI14/01722, PI14/00636, PI14/00618, PI14/00696, PI14/01206, PI14/01919, PI14/00853, PI14/01374, PI16/00473, PI16/00662, PI16/01873, PI16/01094, PI16/00501, PI16/00533, PI16/00381, PI16/00366, PI16/01522, PI16/01120, PI17/00764, PI17/01183, PI17/00855, PI17/01347, PI17/00525, PI17/01827, PI17/00532, PI17/00215, PI17/01441, PI17/00508, PI17/01732, PI17/00926, The Especial Action Project entitled: 'Implementacion y Evaluacion de una intervencion intensiva sobre la actividad fisica Cohorte PREDIMED-Plus' grant to Jordi Salas-Salvado, the Recercaixa grant to Jordi Salas-Salvado (2013ACUP00194), grants from the Consejeria de Salud de la Junta de Andalucia (PI0458/2013; PS0358/2016; PI0137/2018), the PROMETEO/2017/017 grant from the Generalitat Valenciana, the SEMERGEN grant, and CIBEROBN and FEDER funds (CB06/03), ISCIII. International Nut&Dried Fruit Council-FESNAD N degrees 201302: Miguel Angel Martinez-Gonzalez (PI). None of the funding sources took part in the design, collection, analysis or interpretation of the data, or in the decision to submit the manuscript for publication. The corresponding author had full access to all the data in the study and had final responsibility to submit for publication