Oceanic Rossby waves drive inter-annual predictability of net primary production in the central tropical Pacific

In the Pacific Ocean, off-equatorial Rossby waves (RWs), initiated by atmosphere-ocean interaction, modulate the inter-annual variability of the thermocline. In this study, we explore the resulting potential gain in predictability of central tropical Pacific primary production, which in this region strongly depends on the supply of macronutrients from below the thermocline. We use a decadal prediction system based on the Max Planck Institute Earth system model to demonstrate that for the time period 1998-2014 properly initialized RWs explain an increase in predictability of net primary productivity (NPP) in the off-equatorial central tropical Pacific. We show that, for up to 5 years in advance, predictability of NPP derived from the decadal prediction system is significantly larger than that derived from persistence alone, or an uninitialized historical simulation. The predicted signal can be explained by the following mechanism: off-equatorial RWs are initiated in the eastern Pacific and travel towards the central tropical Pacific on a time scale of 2-6 years. On their arrival the RWs modify the depths of both thermocline and nutricline, which is fundamental to the availability of nutrients in the euphotic layer. Local upwelling transports nutrients from below the nutricline into the euphotic zone, effectively transferring the RW signal to the near-surface ocean. While we show that skillful prediction of central off-equatorial tropical Pacific NPP is possible, we open the door for establishing predictive systems for food web and ecosystem services in that region

Costs, and cost-outcome of school feeding programmes and feeding programmes for young children. Evidence and recommendations

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Exploiting graphlet decomposition to explain the structure of complex networks: the GHuST framework

The characterization of topology is crucial in understanding network evolution and behavior. This paper presents an innovative approach, the GHuST framework to describe complex-network topology from graphlet decomposition. This new framework exploits the local information provided by graphlets to give a global explanation of network topology. The GHuST framework is comprised of 12 metrics that analyze how 2- and 3-node graphlets shape the structure of networks. The main strengths of the GHuST framework are enhanced topological description, size independence, and computational simplicity. It allows for straight comparison among different networks disregarding their size. It also reduces the complexity of graphlet counting, since it does not use 4- and 5-node graphlets. The application of the novel framework to a large set of networks shows that it can classify networks of distinct nature based on their topological properties. To ease network classification and enhance the graphical representation of them, we reduce the 12 dimensions to their main principal components. Furthermore, the 12 dimensions are easily interpretable. This enables the connection between complex-network analyses and diverse real applications

European communication networks in the Early Modern Age

Recent contributions to knowledge about early journalism developed in different parts of Europe*Italy, France, England, Germany, the Netherlands, Portugal, Spain . . . *have made it possible to trace a fairly precise map for the historical origin of this phenomenon. However, the scope of work carried out with a view to developing frameworks of interpretation to explain the reasons for this appearance is not as far-reaching. This paper reviews the recurring theoretical models found to date in the specific bibliography and proposes a new framework of interpretation, capable of encompassing the complexity and pan-European nature of early journalism in history

A homogeneous method for investigation of methylation-dependent protein–protein interactions in epigenetics

Methylation of lysine residues on the tails of histone proteins is a major determinant of the transcription state of associated DNA coding regions. The interplay among methylation states and other histone modifications to direct transcriptional outcome is referred to as the histone code. In addition to histone methyltransferases and demethylases which function to modify the methylation state of lysine sidechains, other proteins recognize specific histone methylation marks essentially serving as code readers. While these interactions are highly specific with respect to site and methylation state of particular lysine residues, they are generally weak and therefore difficult to monitor by traditional assay techniques. Herein, we present the design and implementation of a homogeneous, miniaturizable, and sensitive assay for histone methylation-dependent interactions. We use AlphaScreen, a chemiluminescence-based technique, to monitor the interactions of chromodomains (MPP8, HP1β and CHD1), tudor domains (JMJD2A) and plant homeodomains (RAG2) with their cognate trimethyllysine histone partners. The utility of the method was demonstrated by profiling the binding specificities of chromo- and tudor domains toward several histone marks. The simplicity of design and the sensitive and robust nature of this assay should make it applicable to a range of epigenetic studies, including the search for novel inhibitors of methylation-dependent interactions

Spectral information to get beyond color in the analysis of water‑soluble varnish degradation

Spectral images were captured of paper samples varnished with two water-soluble materials: gum arabic and egg white. The samples were submitted to degradation processes that partially or totally eliminated the varnish from the substrate (water immersion and ageing). The spectral information was used to obtain average color data and to characterize the spatial and color inhomogeneity across pixels, showing that the pixel spectral data are critical for an accurate characterization of the degradation process of the varnishes. Since the varnishes typically become yellower with ageing, this study introduces two novel and simple-to-compute yellowness indices based on the spectral information, which are validated against a standard colorimetric index (ASTM-E313 2015). The potential uses of spectral information are demonstrated with several pieces of a real antique map sample by comparing the spectral information measured before and after cleaning the sample. To sum up, the main contributions of this study are the characterization of the spatial homogeneity through pixel-based spectral and color information and the proposal of spectral-based yellowing indices for two critical applications (ageing process follow-up and effect of cleaning), as demonstrated with synthetic and historical samples of varnished paper respectively.Spanish Ministry of Economy and Competitiveness, under research Grant DPI2015-64571-R. Spanish State Agency of Research (AEI) and the Ministry for Economy, Industry and Competitiveness (MIMECO) by means of the Grant Number FIS2017-89258-P with European Union FEDER (European Regional Development Funds) support

Complete genome sequence of <i>Vibrio anguillarum</i> phage CHOED successfully used for phage therapy in aquaculture

Vibrio anguillarum phage CHOED was isolated from Chilean mussels. It is a virulent phage showing effective inhibition of V. anguillarum. CHOED has potential in phage therapy, because it can protect fish from vibriosis in fish farms. Here, we announce the completely sequenced genome of V. anguillarum phage CHOED

Interaction of Proliferation Cell Nuclear Antigen (PCNA) with c-Abl in Cell Proliferation and Response to DNA Damages in Breast Cancer

Cell proliferation in primary and metastatic tumors is a fundamental characteristic of advanced breast cancer. Further understanding of the mechanism underlying enhanced cell growth will be important in identifying novel prognostic markers and therapeutic targets. Here we demonstrated that tyrosine phosphorylation of the proliferating cell nuclear antigen (PCNA) is a critical event in growth regulation of breast cancer cells. We found that phosphorylation of PCNA at tyrosine 211 (Y211) enhanced its association with the non-receptor tyrosine kinase c-Abl. We further demonstrated that c-Abl facilitates chromatin association of PCNA and is required for nuclear foci formation of PCNA in cells stressed by DNA damage as well as in unperturbed cells. Targeting Y211 phosphorylation of PCNA with a cell-permeable peptide inhibited the phosphorylation and reduced the PCNA-Abl interaction. These results show that PCNA signal transduction has an important impact on the growth regulation of breast cancer cells

Trends in yearly prevalence of third-generation cephalosporin and fluoroquinolone resistant Enterobacteriaceae infections and antimicrobial use in Spanish hospitals, Spain, 1999 to 2010

Escherichia coli, Klebsiella pneumoniae, and Enterobacter spp. are a major cause of infections in hospitalised patients. The aim of our study was to evaluate rates and trends of resistance to third-generation cephalosporins and fluoroquinolones in infected patients, the trends in use for these antimicrobials, and to assess the potential correlation between both trends. The database of national point prevalence study series of infections and antimicrobial use among patients hospitalised in Spain over the period from 1999 to 2010 was analysed. On average 265 hospitals and 60,000 patients were surveyed per year yielding a total of 19,801 E. coli, 3,004 K. pneumoniae and 3,205 Enterobacter isolates. During the twelve years period, we observed significant increases for the use of fluoroquinolones (5.8%–10.2%, p<0.001), but not for third-generation cephalosporins (6.4%–5.9%, p=NS). Resistance to third-generation cephalosporins increased significantly for E. coli (5%–15%, p<0.01) and for K. pneumoniae infections (4%–21%, p<0.01) but not for Enterobacter spp. (24%). Resistance to fluoroquinolones increased significantly for E. coli (16%– 30%, p<0.01), for K. pneumoniae (5%–22%, p<0.01), and for Enterobacter spp. (6%–15%, p<0.01). We found strong correlations between the rate of fluoroquinolone use and the resistance to fluoroquinolones, third-generation cephalosporins, or co-resistance to both, for E. coli (R=0.97, p<0.01, R=0.94, p<0.01, and R=0.96, p<0.01, respectively), and for K. pneumoniae (R=0.92, p<0.01, R=0.91, p<0.01, and R=0.92, p<0.01, the use of third-generation cephalosporins and resistance to any of the latter antimicrobials. No significant correlations could be found for Enterobacter spp.. Knowledge of the trends in antimicrobial resistance and use of antimicrobials in the hospitalised population at the national level can help to develop prevention strategiesSupported by the Fondo para la investigación, Spanish Ministry of Health, grant PI07/90255