Effect of Thyroxine and RGH Hormone Recombinant on Growth and Survival Goldfish Larvae, Carassius Auratus (Linnaeus, 1758)

Pertumbuhan yang lambat merupakan kendala dalam mencapai target produksi ikan maskoki (Carassius auratus auratus). Proses metabolisme dan pertumbuhan pada ikan dipengaruhi oleh faktor hormonal. Penelitian ini dilaksa-nakan pada bulan Februari-Maret 2016 di Laboratorium Pembenihan dan Teknologi Budidaya, Perairan Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Malikussaleh. Penelitian ini bertujuan untuk menguji pe-ngaruh pemberian hormon tiroksin (T4) dan hormon rGH (recombinant Growth Hormone) melalui metode peren-daman dengan dosis yang berbeda terhadap pertumbuhan dan sintasan larva ikan maskoki. Metode penelitian ekspe-rimental dengan rancangan acak lengkap non faktorial dengan empat perlakuan tiga ulangan. Adapun perlakuannya adalah: A= Kontrol, B= T4 0,05 mgL-1+ rGH 5 mgL-1, C= T4 0,1 mgL-1+ rGH 10 mgL-1, D = T4 0,15 mgL-1+ rGH 15 mgL-1. Hasil penelitian dengan pemberian rekombinasi hormon T4 dan hormon rGH dengan dosis yang berbeda ber-pengaruh terhadap penurunan volume kuning telur, laju penyerapan kuning telur, pertumbuhan dan sintasan dengan nilai p< 0,05. Perlakuan terbaik terdapat pada perlakuan D (T4 0,15 mg L-1 + rGH 15 mgL-1) dengan hasil volume kuning telur dan laju penyerapan kuning telur selama 36 jam, pertumbuhan bobot : 3,09 mg, pertumbuhan panjang : 1,75 mm, dan sintasan : 81 %. Nilai kualitas air selama penelitian yaitu suhu 27-28 oC, pH 7,0-7,4, oksigen terlarut 5-6,1 mgL-1 dan amonia 0,1073-0,1793 mgL-1, masih mendukung pertumbuhan ikan yang optimal bagi ikan mas koki. Penelitian ini diharapkan mampu mempercepat proses pertumbuhan larva ikan mas koki dengan nilai sintasan yang sangat baik

The Effect of Work Stress and Work Conflict on Employees Turnover Intention in Middle Small Micro Enterprises (MSMEs) in South Tangerang Region

This study aims to determine the effect of work stress and work conflict on the turnover intention of employees of Micro, Small and Medium Enterprises (MSMEs) in the South Tangerang Region. The method used is explanatory research with analysis techniques using statistical analysis with regression testing, correlation, determination, and hypothesis testing. The results of this study work stress have a significant effect on the turnover intention by 37.0%, the hypothesis test is obtained t count&gt; t table or (7,438&gt; 1,986). Work conflict has a significant effect on the turnover intention by 49.9%, hypothesis testing is obtained t count&gt; t table or (9.679&gt; 1.986). Work stress and work conflict simultaneously have a significant effect on turnover intention with the regression equation Y = 9.021 + 0.272X1 + 0.510X2. The contribution of the effect was 55.2%, the hypothesis test obtained F count&gt; F table or (57.286&gt; 2,700)

Penerapan Perilaku Hidup Bersih dan Sehat (Phbs) pada Masyarakat untuk Mencegah Penularan Covid-19 di Wilayah Kota Depok

In the condition of the COVID-19 disease outbreak, this is something that must be stopped, namely by means of the community having access to clean water to always wash their hands with soap and not forgetting to increase their immunity, it is recommended to consume fruits and vegetables accompanied by physical activity every days, and for this smoking behavior, because smoking causes damage to the function of the lungs and respiratory tract of humans, it makes it increasingly difficult for COVID-19 sufferers to take oxygen or risk shortness of breath which can be fatal. This study was conducted with the aim of knowing the general description of the state of public health status based on aspects of implementing Clean and Healthy Behavior in the community to prevent the transmission of Covid-19 in the Depok City Region. This study uses a descriptive quantitative method, namely a study conducted to describe a phenomenon. The data presented in the form of numbers in the form of secondary data collection (Depok Health Profile) and primary data collected using questionnaires from June to August 2021 in the Depok City Community. The population in this study was the entire Depok City Community and the sample in this study was as many as 1,018 people in Depok City. The results of this study showed that 550 (54.02%) informants had family members who smoked, this is a factor affected by Covid-19 in the Depok City Area. The conclusion of this study is that Covid-19 is something that must be stopped, namely smoking behavior, because smokers are one of the sufferers who are very at risk of complications and death from the corona virus

Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing.

Beyond the nearly uniform presence of KRAS mutations, pancreatic cancer is increasingly recognized as a heterogeneous disease. Preclinical in vivo model systems exist, but with the advent of precision oncology, murine models with enhanced genetic flexibility are needed to functionally annotate genetic alterations found in the human malignancy. Here, we describe the generation of focal gene disruptions and large chromosomal deletions via inducible and pancreas-specific expression of Cas9 in adult mice. Experimental mice are derived on demand directly from genetically engineered embryonic stem cells, without the need for further intercrossing. To provide initial validation of our approach, we show that disruption of the E3 ubiquitin ligase Rnf43 accelerates Kras(G12D)-dependent tumourigenesis. Moreover, we demonstrate that this system can be used to rapidly interrogate the impact of complex cancer-associated alleles through the generation of a previously unstudied 1.2 megabase deletion surrounding the CDKN2A and CDKN2B tumour suppressors. Thus, our approach is capable of reproducibly generating biallelic and precise loss of large chromosomal fragments that, in conjunction with mutant Kras, leads to development of pancreatic ductal adenocarcinoma with full penetrance

Murine liver organoids as a genetically flexible system to study liver cancer <em>in vivo</em> and<em> in vitro</em>.

The rising incidence of cholangiocarcinoma (CCA) coupled with a low 5-year survival rate that remains below 10% delineates the urgent need for more effective treatment strategies. Although several recent studies provided detailed information on the genetic landscape of this fatal malignancy, versatile model systems to functionally dissect the immediate clinical relevance of the identified genetic alterations are still missing. To enhance our understanding of CCA pathophysiology and facilitate rapid functional annotation of putative CCA driver and tumor maintenance genes, we developed a tractable murine CCA model by combining the cyclization recombination (Cre)-lox system, RNA interference, and clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) technology with liver organoids, followed by subsequent transplantation into immunocompetent, syngeneic mice. Histologically, resulting tumors displayed cytokeratin 19-positive ductal structures surrounded by a desmoplastic stroma-hallmark features of human CCAs. Despite their initial biliary phenotype organoids retained the plasticity to induce a broader differentiation spectrum of primary liver cancers following transplantation into recipient mice, depending on their genetic context. Thus, the organoid system combines the advantage of using nontransformed, premalignant cells to recapitulate liver tumorigenesis as a multistep process, with the advantage of a reproducible and expandable cell culture system that abrogates the need for recurrent isolations of primary cells. Genetically modified liver organoids are able to transform into histologically accurate CCAs. Depending on the oncogenic context, they are also able to give rise to liver cancers that show features of hepatocellular carcinomas. The model can be used to functionally explore candidate cancer genes of primary liver cancers in immunocompetent animals and evaluate novel treatment regimens