293 research outputs found
Phosphorus speciation in Swedish agricultural clay soils
Phosphorus (P) is an important element for crop production, but build-up of excess soil P can promote P leaching and eutrophication of surface waters. To better understand the dynamics of P release from soil to waters, more knowledge is needed about sorption patterns and P speciation in agricultural soils.
Two new indices were developed to assess the importance of P sorption to hydroxy-interlayered clay minerals, and to evaluate the amount of hydroxy-interlayering and hydroxy-interlayer stability. A strong relationship was found between oxalate-extractable aluminium (Al) and the amount of hydroxy-interlayering in soil, suggesting a common source. This makes it difficult to analytically distinguish between phosphate (PO4) adsorbed to hydroxy-interlayers from PO4 adsorbed to Al hydroxide-type precipitates.
Application of X-ray absorption near-edge structure (XANES) spectroscopy to evaluate P speciation in soil profiles and the effects of P depletion and fertilisation revealed a distinct change in P speciation with increasing soil depth in an agricultural clay soil profile. The results indicated that the subsoil P predominantly occurred as apatite, whereas PO4 adsorbed with Al-hydroxides or hydroxy-interlayers dominated in the topsoil. Organic P and PO4 adsorbed to iron (Fe)-(hydr)oxides were observed only in the topsoil. This can be explained by long-term weathering of apatite and silicates, particularly ferromagnesian forms such as amphibole, in the upper soil horizons, causing an association of the released PO4 with secondary metal (hydr)oxides.
Collectively, the XANES results showed that the most important phases governing P retention and release in agricultural clay soils are adsorption of PO4 to Al and to Fe in (hydr)oxide minerals or interlayers. After fertilisation, P was adsorbed to Al hydroxide phases in six different soil types studied, but in two of the soils there was also an increase in calcium phosphate. Moreover, P solubility was shown to be lowest at pH values ranging from 4.5 to 7.5 and increased with decreasing pH, probably as a result of the dissolution of apatite and PO4-bearing hydroxy-Al precipitates at low pH
Norwegian dairy farmer's preferences for breeding goal traits and associations with herd and farm characteristics
The aims of this study were to investigate variation and clustering in breeding goal trait preferences among Norwegian dairy farmers and to identify factors with a systematic influence on their preferences. An internet-based questionnaire was sent out to dairy farmers connected to the Norwegian co-operative breeding organization Geno (N = 8222), of which 10.8% answered (N = 888). Of the 15 suggested traits fertility had the highest overall ranking, while parasite resistance and methane emission had the lowest. Four distinct preference clusters were identified by the means of cluster analysis, of which two had a high preference for milk production. Differences in terms of farm and herd characteristics between clusters suggests a mixture of systematic and
intrinsic effects on breeding goal trait priorities. This study shows that Norwegian dairy farmers’ preferences for breeding goal traits fall into four distinct clusters, both affected by herd and farm characteristics along with intrinsic value
Digital soil mapping of copper in Sweden: Using the prediction and uncertainty as decision support in crop micronutrient management
Digital soil mapping (DSM) of topsoil copper (Cu) concentrations and prediction intervals covering 90% of agricultural land in Sweden was performed, in order to identify areas at risk of Cu deficiency. A total of 12,527 soil samples were used to calibrate the DSM model, using airborne gamma radiation data, climate data, topographical data and soil texture class data. Among the samples included, 11,093 had no laboratory-analysed Cu concentrations, so their Cu concentrations were predicted using portable X-ray fluorescence (PXRF) measurements. Cross-validation of the PXRF model resulted in Nash-Sutcliffe model efficiency coefficient (E) of 0.66 and mean absolute error (MAE) of 3.3 mg kg−1. Cross-validation of the DSM model showed somewhat lower performance (E = 0.57, MAE = 4.1 mg kg−1). Based on the lower bound of the prediction interval (5th percentile), 48% of agricultural soils in Sweden are most likely not at risk of Cu deficiency (>7 mg kg−1). The Cu map was also validated against concentrations in soil samples from five fields (25–47 ha in size; four samples per ha). The field means were predicted with a MAE of 1.0 mg kg−1 and within-field variation was reproduced with a field-wise squared Pearson correlation coefficient (r2) of 0–0.36. The classification metric ‘recall’ showed that the map of soil Cu concentrations might not predict all possible areas at risk of being Cu deficient, as observational data indicates that about 22% of soils in the mapped area should have Cu concentrations below the risk limit. However, the metric ‘precision’ showed that when the soil map predicted a concentration at or below 7 mg kg−1, it was generally correct. Increasing the limit resulted in the recall and precision increasing rapidly. The remaining 52% of agricultural soils at risk of being below the Cu concentration limit can be targeted by laboratory analysis or monitoring
Phosphorus in agricultural soils around the Baltic Sea
Eutrophication of the Baltic Sea is a serious problem. A major contributing factor is diffuse losses of phosphorus (P) from agricultural land in surrounding countries. In order to estimate P losses, environmental monitoring of small agriculture-dominated catchments is being carried out in most of these countries. Evaluation of the risk of P leaching to waters is usually based on chemical tests originally developed to quantify the amount of soil P available for plant production. The tests are performed in different ways in the different countries and a number of different extraction agents are in use. The ammonium lactate method (P-AL) is used in Sweden and Lithuania, the double lactate method (P-DL) in Latvia and Poland, the Mehlich 3 method (P-M3) in Estonia and the Olsen method (P-Olsen) in Denmark.
A total of 99 soil samples from five agricultural catchments and two field trials in the Baltic States and Sweden were extracted according to the four methods listed above. The amount of P was then quantified either colorimetrically or by inductively coupled plasma (ICP) spectrometry in accordance with the practices of the respective country. The amount of P determined by ICP spectrometry was nearly always (in 98% of cases) higher than that determined colorimetrically, with an average difference of 19% in plant-available P. The amount of P extracted by the four methods increased in the order Olsen-P < P-DL ≤ P-M3 < P-AL, with Olsen-P values being on average only 24% of P-AL values. The different active agents used in the four methods differ in their efficiency in desorbing and releasing P from minerals and organic compounds.
In the Baltic Sea area, neither a P adsorption index (PSI) or the amounts of P in relation to aluminium (Al-AL) and iron (Fe-AL) in the acidic AL extract is suggested to be a general good predictor of soil capacity to adsorb P or release dissolved reactive P (DRP) to water, based on the results from the limited number of sites in the present study
Validation of the rheumatoid arthritis diagnosis in the Swedish National patient register: a cohort study from Stockholm County
Flawed risk assessment of antifouling paints leads to exceedance of guideline values in Baltic Sea marinas
The seasonal variations of dissolved and bioavailable copper (Cu) and zinc (Zn) were studied in two recreational marinas in Sweden and Finland. The time series from the two marinas were characterized by rising concentrations during the spring boat launching, elevated concentrations all through the peak boating season, and decreasing concentrations in autumn when boats were retrieved for winter storage. This pattern shows a clear link between Cu and Zn concentrations and boating activity, with antifouling paints as the principal source. The leaching from antifouling paints was also found to significantly alter the speciation of dissolved Cu and Zn in marina waters, with an increase of the proportion of metals that may be considered bioavailable. This change in speciation, which occurred without any change in dissolved organic carbon (DOC), further increases the environmental risk posed by antifouling paints. In the Swedish marina, dissolved Cu and Zn exceed both Environmental Quality Standards (EQS) and Predicted No Effect Concentrations (PNEC), indicating that the current Swedish risk assessment (RA) of antifouling paints is failing to adequately protect the marine environment. An evaluation of the RA performance showed the underlying cause to be an underestimation of the predicted environmental concentration (PEC) by factors of 2 and 5 for Cu and Zn, respectively. For both metals, the use of inaccurate release rates for the PEC derivation was found to be either mainly (Cu) or partly (Zn) responsible for the underestimation. For Zn, the largest source of error seems to be the use of an inappropriate partitioning coefficient (KD) in the model. To ensure that the use of antifouling coatings does not adversely impact the sensitive Baltic Sea, it is thus recommended that the KD value for Zn is revised and that representative release rates are used in the RA procedure
Keratins Stabilize Hemidesmosomes through Regulation of β4-Integrin Turnover
Epidermal integrity and wound healing depend on remodeling of cell-matrix contacts including hemidesmosomes. Mutations in β4-integrin and plectin lead to severe epidermolysis bullosa (EB). Whether mutations in keratins K5 or K14, which cause EB simplex, also compromise cell-matrix adhesion through altering hemidesmosomal components is not well investigated. In particular, the dependence of β4-integrin endocytosis and turnover on keratins remains incompletely understood. Here, we show that the absence of keratins causes loss of plectin-β4-integrin interaction and elevated β4-integrin phosphorylation at Ser1354 and Ser1362. This triggered a caveolin-dependent endocytosis of β4-integrin but not of other integrins through Rab5 and Rab11 compartments in keratinocytes. Expressing a phospho–deficient β4-integrin mutant reduces β4-integrin endocytosis and rescues plectin localization in keratin–free cells. β4-integrin phosphorylation in the absence of keratins resulted from elevated Erk1/2 activity downstream of increased EGFR and PKCα signaling. Further, increased Erk1/2 phosphorylation and altered plectin localization occur in keratin–deficient mouse epidermis in vivo. Strikingly, expression of the K14-R125P EBS mutant also resulted in plectin mislocalization and elevated β4-integrin turnover, suggesting disease relevance. Our data underscore a major role of keratins in controlling β4-integrin endocytosis involving a plectin-Erk1/2-dependent mechanism relevant for epidermal differentiation and pathogenesis
Sensor mapping of amazonian dark earths in deforested croplands
Amazonian Dark Earths (ADEs) are fertile soils for agricultural production as well as important archaeological resources for understanding the pre-Columbian past of the Neotropical lowland rainforest. ADEs are threatened by expanding land exploitation and there is a need to develop efficient approaches to soil mapping and analysis for documenting these soils. In this paper we assess the potential of satellite remote sensing and proximal soil sensing to map, predict and monitor ADEs in land affected by agro-industrial development. We use instruments based on portable x-ray fluorescence (PXRF) and electromagnetic induction (EMI) as well as high-resolution satellite data (Spot 6) for detailed soil surveys at a 10-ha ADE site now mainly used for soybean production on the Belterra Plateau, Pará, Brazil. We predict the regional occurrence of ADE in a c. 250 km 2 test area centred on the known ADE site São Francisco using satellite data. Multivariate adaptive regression splines models were parameterised for predictions of soil organic carbon (SOC), cation exchange capacity (CEC), phosphorus (P) and depth of the A horizon in ADEs from sensor data - both from individual sensors and in sensor combinations. Combining sensors gave the best validation results: the highest modelling efficiencies (E) were 0.70 (SOC), 0.88 (CEC) and 0.74 (for both P and A depth). The most powerful single proximal sensor outputs in the predictions were Sr from the PXRF data and magnetic susceptibility (MSa) as measured by the EMI instrument. In the regional satellite based model we located 17 previously unrecorded ADE sites N2 ha. Ground control checks showed that 10 out of 11 sites were correctly classified. We conclude that these sensors are useful in studies of ADE in deforested cropland and provide new opportunities for detailed studies of the archaeological record
Validation of Effective Extracellular Vesicles Isolation Methods Adapted to Field Studies in Malaria Endemic Regions.
Malaria affects the poorer regions of the world and is of tremendous health and economic burden for developing countries. Extracellular vesicles (EVs) are small vesicles released by almost any cells in the human body, including malaria infected red blood cells. Recent evidence shows that EVs might contribute to the pathogenesis of malaria. In addition, EVs hold considerable value in biomarker discovery. However, there are still significant gaps in our understanding of EV biology. So far most of our knowledge about EVs in malaria comes from in vitro work. More field studies are required to gain insight into their contribution to the disease and pathogenesis under physiological conditions. However, to perform research on EVs in low-income regions might be challenging due to the lack of appropriate equipment to isolate EVs. Therefore, there is a need to develop and validate EV extraction protocols applicable to poorly equipped laboratories. We established and validated two protocols for EV isolation from cell culture supernatants, rodent and human plasma. We compared polyethylene glycol (PEG) and salting out (SA) with sodium acetate for precipitation of EVs. We then characterized the EVs by Transmission Electron Microscopy (TEM), Western Blot, Size-exclusion chromatography (SEC), bead-based flow cytometry and protein quantification. Both protocols resulted in efficient purification of EVs without the need of expensive material or ultracentrifugation. Furthermore, the procedure is easily scalable to work with large and small sample volumes. Here, we propose that both of our approaches can be used in resource limited countries, therefore further helping to close the gap in knowledge of EVs during malaria
Evolution of phosphorus speciation with depth in an agricultural soil profile
With time, different soil-forming processes such as weathering, plant growth, accumulation of organic matter, and cultivation are likely to affect phosphorus (P) speciation. In this study, the depth distribution of P species was investigated for an agricultural clay soil, Lanna, Sweden. Small amounts of apatite-P was demonstrated in the topsoil whereas the speciation of Pat 70-100 cm depth consisted of approximately 86% apatite according to P K-edge XANES (X-ray absorption near-edge structure) spectroscopy. Because there were only minor differences in bulk mineralogy and texture, these variations in P speciation were interpreted as the result of apatite weathering of the topsoil. Speciation modeling on soil extracts supported this idea: hydroxyapatite was not thermodynamically stable in the top 50 cm of the soil. Apatite was enriched in the bulk soil relative to the clay fraction, as expected during apatite dissolution. Combined results from batch experiments, XANES spectroscopy and X-ray diffraction suggested chemical transformations of the topsoil as a result from accumulation of organic matter and airing from tillage followed by enhanced weathering of apatite, amphiboles, clay minerals, and iron oxides. This caused the formation of poorly crystalline secondary iron and aluminum (hydr)oxides in the topsoil, which retained part of the released P from apatite. Other P was incorporated into organic forms. Furthermore, the results also showed that short-term acidification below the current pH value (below 5.5 in the topsoil and 7.2 in the deeper subsoil) caused significant solubilization of P. This is attributed to two different mechanisms: the instability of Al-containing sorbents (e.g. Al hydroxides) at low pH (in the topsoil), and the acid-mediated dissolution of apatite (the subsoil). (C) 2016 Elsevier B.V. All rights reserved
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