A novel approach to determine anti-proliferative, anti-migratory and anti-microbial properties of 2-phenylethylammonium carboxylate molecular salts

2-Phenylethylammonium (PEA) salt derivatives have been prepared between 2-phenylethylamine with various aromatic carboxylic acids (nicotinic, benzoic, salicylic, and γ-resorcylic) and confirmed by spectroscopic analyses. Additionally, the possible biological activities of these salts have been examined and the antiproliferative and antimigrative effects of salts on breast and colon cancer cell lines have been determined. Furthermore, to obtain preliminary data for the effects of the indicated compounds on cancer angiogenesis, in vitro migration analysis has been performed by human vascular endothelial cells. The antibacterial effects of the compounds have also been investigated. Therefore, an important contribution has been made to studies conducted to better recognize the pharmacological effects of salts

Investigation of relationship between IL-6 gene variants and hypertension in Turkish population

Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a polymorphic gene which -174 G/C is a common and -572 G/C is a rare polymorphisms identified in promoter region. Publications on IL-6 gene polymorphisms raised the question whether this gene polymorphisms lead to susceptibility to HT or not. To investigate the effects of IL-6 gene -174 G/C (rs 1800795) and -572 G/C (rs1800796) polymorphisms on plasma IL-6 and CRP levels and their associations with hypertension disease in Turkish population we analyzed -174 G/C and -572 G/C polymorphisms and plasma IL-6 and CRP levels in 111 healthy controls and 108 hypertension patients from Adyaman, Turkey. We determined the genotypes using polymerase chain reaction-restriction fragment length polymorphism and analyzed plasma levels of IL-6 by ELISA and CRP by automated standard biochemical methods. We have found no statistically significant differences between IL-6 gene -174 G/C and -572 G/C genotypes and allelic frequencies and IL-6 and CRP plasma levels and HT (p > 0.05). No CC genotype was found in control subjects for -572 G/C polymorphism. In conclusion, we found relation to -174 G/C and -572 G/C gene variants between neither IL-6 and CRP levels nor hypertension. The -572 G allele and GG genotype are predominant in Turkish population in Adyaman, Turkey whereas the CC genotype is very rare.This study was supported by a grant of the Research Foundation of Adiyaman University (FEFYL/2012-0002), Turkey

Study of Turkish Preschool Teachers' Attitudes toward Science Teaching

This study aims to explore preschool teachers' attitudes toward science teaching and its impact on classroom practices through the frequency of science activities provided in the classroom. In addition, the study investigates if their attitudes are related to factors such as educational level, years of teaching experience, and the school type they work in. The present research was conducted with 292 preschool teachers who work in public and private schools in different districts of Ankara, Turkey. The data were collected by administering the Early Childhood Teachers' Attitudes toward Science Teaching Scale. Our analyses indicate that there is a significant but weak link between preschool teachers' attitudes toward science teaching and the frequency of science activities that they provide in the classroom. Further, while teachers' characteristics such as educational level and experience are found to play an insignificant role on the overall measures of the scale, type of school appears to be a major factor in explaining the attitudes toward science teaching

A novel approach to determine anti-proliferative, anti-migratory and anti-microbial properties of 2-phenylethylammonium carboxylate molecular salts

207-2162-Phenylethylammonium (PEA) salt derivatives have been prepared between 2-phenylethylamine with various aromatic carboxylic acids (nicotinic, benzoic, salicylic, and γ-resorcylic) and confirmed by spectroscopic analyses. Additionally, the possible biological activities of these salts have been examined and the antiproliferative and antimigrative effects of salts on breast and colon cancer cell lines have been determined. Furthermore, to obtain preliminary data for the effects of the indicated compounds on cancer angiogenesis, in vitro migration analysis has been performed by human vascular endothelial cells. The antibacterial effects of the compounds have also been investigated. Therefore, an important contribution has been made to studies conducted to better recognize the pharmacological effects of salts

Seroprevalence of Asymptomatic Leishmania spp. Carriage Among Blood Donors in Leishmaniasis Endemic Area in Turkey

Introduction: Transfusion-related infections are usually caused by a microbial pathogen transmitted to the recipient by the donated blood. Plasmodium spp., Trypanosoma cruzi, Babesia microti, Toxoplasma gondii and Leishmania spp. are listed as the most widely reported transfusion-transmitted parasites. Leishmaniasis is well known as an endemic in Mediterranean countries including Turkey. Accordingly, detection of asymptomatic Leishmania infantum carriage in blood donors is an important issue in Turkey. In endemic teretories, research on blood donors is under-represented in Turkey. Likewise, Mersin province is also endemic for Leishmaniasis. Up to date, no studies have been conducted to detect Leishmaniasis in healthy blood donors in our region. Therefore, the main objective of the current study was to reveal the seroprevalance of asymptomatic Leishmania carriage among the blood donors in Mersin province

Koroner arter bypass cerrahisinde ramiprilin miyokardiyal hasar ve inflamatuvar yanıttaki etkisi üzerinde sitokin gen polimorfizmlerinin rolü

Purpose: Ramipril is effective in treating inflammatory myocardial injury by reducing cytokines such as TNF-?, IL-6, and IL-8. In this study, we investigated the effect of cytokine gene polymorphisms on inflammatory response which might be reduced by ramipril. Material and Methods: Of 102 patients undergoing on-pump coronary artery bypass grafting surgery, 51 were the treatment group which received ramipril and the remaining 51 were the non-treated control group. TNF-?, IL-6, and IL-8 were measured with Enzyme-Linked Immuno Sorbent Assay (ELISA), before anesthesia induction (t1), at 20 min following cross clamping (t2), at the end of the operation (t3), and 24 hours after anesthesia (t4). Genotyping was performed with PCR method. Results: While TNF-? increase began with surgery, IL-6 and IL-8 increase began with cardiac arrest during cardiopulmonary bypass and continued until the end of the operation. In contrast, only IL-8 remained high in the control group during the postoperative period, while TNF-?, IL-6, and IL-8 began to decrease. The decreases in IL-6 at t3 and in TNF-? at t4 were significant. Conclusion: Ramipril might have a role in preventing inflammatory myocardial injury by reducing cytokine and TnT levels after cardiac arrest.Amaç: Ramipril, inflamatuar miyokardial hasarın tedavisinde TNF-?, IL-6 ve IL-8 gibi sitokin seviyelerini azaltarak etkili olmaktadır. Çalışmamızda, ramipril tedavisinin, koroner arter bypass cerrahisi esnasında oluşan inflamatuar yanıt ve miyokardiyal hasara yönelik etkisi üzerinde, sitokin gen polimorfizmlerinin herhangi bir rolü olup olmadığını araştırdık. Gereç ve Yöntem: Çalışmamıza, koroner arter bypass cerrahisi uygulanan hastalardan cerrahi öncesi ramipril alan 51, kontrol grubu olarak ise ramipril almayan 51 olmak üzere toplam 102 hasta dahil edildi. Her iki grupta, anestezi başlamadan hemen önce (t1), kros klemp sonrası 20. dakikada (t2), ameliyatın sonunda (t3) ve anestezinin başlangıcından 24 saat sonra (t4) alınan kan örneklerinden TNF?, IL-6, IL-8 ve TnT serum düzeyleri ELISA yöntemi ile, genotipleme ise PCR-RFLP yöntemleri ile belirlendi. Bulgular: Her iki grupta TNF-? artışı cerrahi ile başlarken, IL-6 ve IL-8 artışı kardiyopulmoner bypass sırasında kardiyak arrestle başlayıp ameliyat sonuna kadar devam etmiştir. Postoperatif dönemde ise sadece IL-8 kontrol grubunda yüksek kalırken; çalışma grubunda TNF-?, IL-6, IL-8 düşüşe geçmektedir. t3 zaman aralığında IL-6, t4 zamanında TNF? değerlerinde anlamlı azalma gözlenmiştir. Sonuç: Ramiprilin kardiyak arrest sonrası sitokin ve TnT düzeylerini azaltarak inflamatuar miyokardiyal hasarı önlemede yeri olabilir