Human monocytes tolerant to LPS retain the ability to phagocytose bacteria and generate reactive oxygen species

Tolerance to lipopolysaccharide (LPS) occurs when animals or cells exposed to LPS become hyporesponsive to a subsequent challenge with LPS. This mechanism is believed to be involved in the down-regulation of cellular responses observed in septic patients. The aim of this investigation was to evaluate LPS-induced monocyte tolerance of healthy volunteers using whole blood. The detection of intracellular IL-6, bacterial phagocytosis and reactive oxygen species (ROS) was determined by flow cytometry, using anti-IL-6-PE, heat-killed Staphylococcus aureus stained with propidium iodide and 2',7'-dichlorofluorescein diacetate, respectively. Monocytes were gated in whole blood by combining FSC and SSC parameters and CD14-positive staining. The exposure to increasing LPS concentrations resulted in lower intracellular concentration of IL-6 in monocytes after challenge. A similar effect was observed with challenge with MALP-2 (a Toll-like receptor (TLR)2/6 agonist) and killed Pseudomonas aeruginosa and S. aureus, but not with flagellin (a TLR5 agonist). LPS conditioning with 15 ng/mL resulted in a 40% reduction of IL-6 in monocytes. In contrast, phagocytosis of P. aeruginosa and S. aureus and induced ROS generation were preserved or increased in tolerant cells. The phenomenon of tolerance involves a complex regulation in which the production of IL-6 was diminished, whereas the bacterial phagocytosis and production of ROS was preserved. Decreased production of proinflammatory cytokines and preserved or increased production of ROS may be an adaptation to control the deleterious effects of inflammation while preserving antimicrobial activity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Divisão de Moléstias InfecciosasUNIFESP, EPM, Divisão de Moléstias InfecciosasFAPESP: 2006/58744-1SciEL

The Near Infrared NaI Doublet Feature in M Stars

The NaI near-infrared doublet has been used to indicate the dwarf/giant population in composite systems, but its interpretation is still a contentious issue. In order to understand the behaviour of this controversial feature, we study the observed and synthetic spectra of cool stars. We conclude that the NaI infrared feature can be used as a dwarf/giant discriminator. We propose a modified definition of the NaI index by locating the red continuum at 8234 angstrons and by measuring the equivalent width in the range 8172-8197 angstrons, avoiding the region at lambda > 8197 angstrons, which contains VI, ZrI, FeI and TiO lines. We also study the dependence of this feature on stellar atmospheric parameters.Comment: 9 pages, (TeX file) + 7 Figures in Postscript format. Accepted for publication in The Astrophysical Journa

Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood

Lipopolysaccharide (LPS) activates neutrophils and monocytes, inducing a wide array of biological activities. LPS rough (R) and smooth (S) forms signal through Toll-like receptor 4 (TLR4), but differ in their requirement for CD14. Since the R-form LPS can interact with TLR4 independent of CD14 and the differential expression of CD14 on neutrophils and monocytes, we used the S-form LPS from Salmonella abortus equi and the R-form LPS from Salmonella minnesota mutants to evaluate LPS-induced activation of human neutrophils and monocytes in whole blood from healthy volunteers. Expression of cell surface receptors and reactive oxygen species (ROS) and nitric oxide (NO) generation were measured by flow cytometry in whole blood monocytes and neutrophils. The oxidative burst was quantified by measuring the oxidation of 2',7'-dichlorofluorescein diacetate and the NO production was quantified by measuring the oxidation of 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate. A small increase of TLR4 expression by monocytes was observed after 6 h of LPS stimulation. Monocyte CD14 modulation by LPS was biphasic, with an initial 30% increase followed by a 40% decrease in expression after 6 h of incubation. Expression of CD11b was rapidly up-regulated, doubling after 5 min on monocytes, while down-regulation of CXCR2 was observed on neutrophils, reaching a 50% reduction after 6 h. LPS induced low production of ROS and NO. This study shows a complex LPS-induced cell surface receptor modulation on human monocytes and neutrophils, with up- and down-regulation depending on the receptor. R- and S-form LPS activate human neutrophils similarly, despite the low CD14 expression, if the stimulation occurs in whole blood.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo (UNIFESP) Disciplina de InfectologiaMax-Planck-Institute for ImmunobiologyUNIFESP, Disciplina de InfectologiaFAPESP: 2006/58744-1SciEL

Near-infrared Spectral Features in Single-aged Stellar Populations

Synthetic spectra for single-aged stellar populations of metallicities [M/H] = -0.5, 0.0 and +0.5, ages = 3 to 17 Gyrs, and initial mass function exponents x = 0.1 to 2.0 were built in the wavelength range 6000-10200 Angstrons. For such we have employed the grid of synthetic spectra described in Schiavon & Barbuy (1999), computed for the stellar parameters 2500 <= Teff <= 6000 K, -0.5 <= log g <= 5.0, [M/H] = -0.5, 0.0 and +0.5, and [alpha/Fe] = 0.0, together with the isochrones by Bertelli et al. (1994) and Baraffe et al. (1998). The behavior of the features NaI8190, CaII8662, TiO6600 and FeH9900 in the integrated spectra of single stellar populations were studied in terms of metallicity, initial mass function and age variations. The main conclusions are that the NaI doublet is an IMF-sensitive feature, which is however sensitive also to metallicity and age, whereas TiO, CaII and FeH are very sensitive to metallicity and essentially insensitive to IMF and age.Comment: 13 pages + 7 figures, ApJ accepte

Fabry-Perot Absorption Line Spectroscopy of the Galactic Bar. II. Stellar Metallicities

We measure the Ca II 8542 line strength in 3360 stars along three lines-of-sight in the Galactic bar: (l,b) ~ (+/-5.0,-3.5) and Baade's Window, using Fabry-Perot (FP) absorption line spectroscopy. This is the first attempt to show that reliable absorption line strengths can be measured using FP spectroscopy. The Ca II 8542 line is a good indicator of metallicity and its calibration to [Fe/H] is determined for globular cluster red giants in previous investigations. We derive such a calibration for the bulge giants and use it to infer metallicities for our full red clump sample (2488 stars) at all three lines-of-sight. We present the stellar metallicity distributions along the major axis of the bar. We find the mean [Fe/H] = -0.09 +/- 0.04 dex in Baade's Window, and find the distribution in this field to agree well with previous works. We find gradients in the mean metallicity and its dispersion w.r.t Baade's WIndow of -0.45 and -0.20 dex respectively at l = +5.5, and of -0.10 dex and -0.20 dex at l = -5.0. We detect a signature of a possible tidal stream at l = +5.5, in both our velocity and metallicity distributions. Its radial velocity indicates that it is not associated with the Sagittarius stream. We also measure the metallicity of a bulge globular cluster NGC 6522 in our Baade's Window field to be -0.90 +/- 0.10 dex, in agreement with recent measurements of Zoccali et al. (2008). This agreement demonstrates the reliability of our metallicity measurements.Comment: accepted for publication in the Astrophysical Journal, 15 pages, 12 figure

The Temperature Scale of Metal-Rich M Giants Based on TiO Bands: Population Synthesis in the Near Infrared

We have computed a grid of high resolution synthetic spectra for cool stars (2500<Teff<6000 K) in the wavelength range 6000 -- 10200A, by employing an updated line list of atomic and molecular lines, together with state-of-the-art model atmospheres. As a by-product, by fitting TiO bandheads in spectra of well-known M giants, we have derived the electronic oscillator strengths of the TiO gamma prime, delta, epsilon and phi systems. The derived oscillator strenghts for the gamma prime, epsilon and phi systems differ from the laboratory and ab initio values found in the literature, but are consistent with the model atmospheres and line lists employed, resulting in a good match to the observed spectra of M giants of known parameters. The behavior of TiO bands as a function of the stellar parameters Teff, log g and [Fe/H] is presented and the use of TiO spectral indices in stellar population studies is discussed.Comment: ApJ accepted, 27 pages + 11 figures, AASLatex v4.

The Infrared Ca II triplet as metallicity indicator

From observations of almost 500 RGB stars in 29 Galactic open and globular clusters, we have investigated the behaviour of the infrared Ca II triplet (8498, 8542 and 8662 \AA) in the age range 13$\leq$Age/Gyr$\leq$0.25 and the metallicity range $-2.2\leq$ [Fe/H] $\leq$+0.47. These are the widest ranges of ages and metallicities in which the behaviour of the Ca II triplet lines has been investigated in a homogeneous way. We report the first empirical study of the variation of the CaII triplet lines strength, for given metallicities, with respect to luminosity. We find that the sequence defined by each cluster in the Luminosity-$\Sigma$Ca plane is not exactly linear. However, when only stars in a small magnitude interval are observed, the sequences can be considered as linear. We have studied the the Ca II triplet lines on three metallicities scales. While a linear correlation between the reduced equivalent width ($W'_V$ or $W'_I$) versus metallicity is found in the \citet{cg97} and \citet{ki03} scales, a second order term needs to be added when the \citet{zw84} scale is adopted. We investigate the role of age from the wide range of ages covered by our sample. We find that age has a weak influence on the final relationship. Finally, the relationship derived here is used to estimate the metallicities of three poorly studied open clusters: Berkeley 39, Trumpler 5 and Collinder 110. For the latter, the metallicity derived here is the first spectroscopic estimate available.Comment: 52 pages, 16 figures, accepted for publication in Astronomical Journa

Spatially resolved Spectro-photometry of M81: Age, Metallicity and Reddening Maps

In this paper, we present a multi-color photometric study of the nearby spiral galaxy M81, using images obtained with the Beijing Astronomical Observatory 60/90 cm Schmidt Telescope in 13 intermediate-band filters from 3800 to 10000{\AA}. The observations cover the whole area of M81 with a total integration of 51 hours from February 1995 to February 1997. This provides a multi-color map of M81 in pixels of 1\arcsec.7 \times 1\arcsec.7. Using theoretical stellar population synthesis models, we demonstrate that some BATC colors and color indices can be used to disentangle the age and metallicity effect. We compare in detail the observed properties of M81 with the predictions from population synthesis models and quantify the relative chemical abundance, age and reddening distributions for different components of M81. We find that the metallicity of M81 is about $Z=0.03$ with no significant difference over the whole galaxy. In contrast, an age gradient is found between stellar populations of the central regions and of the bulge and disk regions of M81: the stellar population in its central regions is older than 8 Gyr while the disk stars are considerably younger, $\sim 2$ Gyr. We also give the reddening distribution in M81. Some dust lanes are found in the galaxy bulge region and the reddening in the outer disk is higher than that in the central regions.Comment: Accepted for publication in AJ (May 2000 issue). 27 pages including 6 figures. Uses AASTeX aasms4 styl

Metallicity Estimates for Old Star Clusters in M33

Using the theoretical stellar population synthesis models of BC96, Kong et al. (2003) showed that some BATC colors and color indices could be used to disentangle the age and metallicity effect. They found that there is a very good relation between the flux ratio of L_{8510}/L_{9170} and the metallicity for stellar populations older than 1 Gyr. In this paper, based on the Kong et al. results and on the multicolor spectrophotometry of Ma et al. (2001, 2002a,b,c), we estimate the metallicities of 31 old star clusters in the nearby spiral galaxy M33, 23 of which are ``true'' globular clusters. The results show that most of these old clusters are metal poor. We also find that the ages and metal abundance for these old star clusters of M33 do not vary with deprojected radial position.Comment: Accepted for Publication in A&A, 13 pages, 7 figures (1 figure of jpg

A library of high resolution synthetic stellar spectra from 300nm to 1.8 micron with solar and alpha-enhanced composition

Libraries of stellar spectra are fundamental tools for the study of stellar populations and both empirical and synthetic libraries have been used for this purpose. In this paper, a new library of high resolution synthetic spectra is presented, ranging from the near-ultraviolet (300nm) to the near-infrared (1.8${\rm \mu}$m). The library spans all the stellar types that are relevant to the integrated light of old and intermediate-age stellar populations in the involved spectral region (spectral types F through M and all luminosity classes). The grid was computed for metallicities ranging from [Fe/H] = --2.5 to +0.5, including both solar and $\alpha$-enhanced ([$\alpha$/Fe] = 0.4) chemical compositions. The synthetic spectra are a good match to observations of stars throughout the stellar parameter space encompassed by the library and over the whole spectral region covered by the computations.Comment: A&A, accepted (revised version