Solid-phase microextraction/gas chromatography–mass spectrometry method optimization for characterization of surface adsorption forces of nanoparticles

A complete characterization of the different physical chemical properties of nanoparticles (NPs) is necessary for the evaluation of their impact on health and environment. Among these properties, the surface characterization of the nanomaterial is the least developed and in many cases limited to the measurement of surface composition and Zeta potential. The biological surface adsorption index approach (BSAI) for characterization of surface adsorption properties of nanoparticles (NPs) has been recently introduced [1,2]. BSAI approach offers in principle the possibility to characterize the different interaction forces exerted between a nanomaterial surface and an organic –and by extension biological- entity. The present work develops further the BSAI approach of and optimizes a solid-phase microextraction – gas chromatography mass spectrometry (SPME/GC-MS) method, which is applied to measure the adsorption properties of different nanomaterials taking into account their specific surface area. This approach gives thus a better defined quantification of the adsorption properties on NPs. To optimize the SPME/GC-MS method, we investigated the various aspects of the process including: kinetics of adsorption of probe compounds on SPME fiber, kinetic of adsorption of probe compounds on NPs surface, and optimization of NPs concentration. The optimized conditions were then tested on 33 probe compounds and on Au NPs (15 nm) and SiO2 NPs (50 nm). The results demonstrated that this detailed optimization of the SPME/GC-MS method under various conditions is a critical factor and pre-requisite to the application of BSAI approach as a tool to characterize surface adsorption properties of NPs and therefore to any further conclusions on their potential impact on health.JRC.I.4-Nanobioscience

Komparativna analiza sadržaja Pb, Fe i Cu u plodovima šipka u zavisnosti od lokaliteta

The area of Herzegovina is known for its intensive cultivation of pomegranate, which is placed on the market either as a fruit or as a processed product. Main task of this research is to determine to which degree agricultural land is contaminated with heavy metals and organic pollutants on the wider area of Mostar and to record the amount of heavy metals that is accumulated in a ripe fruit. Determining of the level of lead, iron and copper in the ripe fruit of pomegranate, the clean grain, and the grain with mesocarp. The research is carried out at three different localities, depending from the place of cultivation from highway M17 and local roads. The same heavy metals were determined in the soil at two different depths.Područje Hercegovine poznato je po intenzivnoj proizvodnji šipka, koji se u sirovom ili prerađenom stanju plasira na tržište. Osnovni zadatak ovog istraživanja je utvrditi stepen kontaminiranosti poljoprivrednog zemljišta teškim metalima i organskim zagađivačima na širem području grada Mostara, te evidentirati količinu teških metala koja se akumulira u zrelim plodovima. Određivanje sadržaja olova, željeza i bakra u zrelim polodovima šipka, čisto zrno i zrno sa mezokarpom. Istraživanje je izvedeno na tri različita lokaliteta, zavisno od udaljenosti mesta uzgoja od magistralnog puta M-17 i lokalnih puteva. Isti teški metali su određivani i u zemljištu na dvema različitim dubinama

Tehnološka svojstva džema od kupine na tržištu grada Mostara

The goal of this paper is the control of the blackberry jam quality. Sampling was carried out by randomly. We carried out chemical, microbiological and organoleptic analysis of the sample. By chemical, microbiological and organoleptic analysis, we have obtained a percentage values of certain elements and it was compared to relevant conditions stipulated in the Rulebook on the quality of products. In order to comply with the given goal, we have analysed three different sample of blackberry jam produced by the following manufacturers Vegafruit, Hepok and Vitaminka. The conclusion is that, during the period of the analysis, the samples were in accordance with the provisions of Rulebook on the quality of fruit and vegetables products.Cilj rada je kontrola kvaliteta džema od kupine koji se nalazi u prodaji na tržištu grada Mostara. Uzorkovanje je izvršeno slučajnim odabirom. Urađene su hemijske, mikrobiološke i organoleptičke analize uzoraka. Pomoću hemijskih, mikrobioloških i organoleptičkih analiza dobila se slika procentualnih vrednosti pojedinih elemenata i uporedila sa odgovarajićim uslovima Pravilnika o kvaliteti proizvoda. Da bi se udovoljilo zadanom cilju analizirana su tri uzorka džema od kupine proizvođača Vegafruit, Hepok i Vitaminka. Zaključak je da su uzorci u periodu analize u skladu sa odredbama važećeg Pravilnika o kvaliteti proizvoda od voća i povrća

Solid-phase microextraction/gas chromatography–mass spectrometry method optimization for characterization of surface adsorption forces of nanoparticles

A complete characterization of the different physico-chemical properties of nanoparticles (NPs) is necessary for the evaluation of their impact on health and environment. Among these properties, the surface characterization of the nanomaterial is the least developed and in many cases limited to the measurement of surface composition and zetapotential. The biological surface adsorption index approach (BSAI) for characterization of surface adsorption properties of NPs has recently been introduced (Xia et al. Nat Nanotechnol 5:671–675, 2010; Xia et al. ACS Nano 5(11):9074–9081, 2011). The BSAI approach offers in principle the possibility to characterize the different interaction forces exerted between a NP's surface and an organic—and by extension biological—entity. The present work further develops the BSAI approach and optimizes a solid-phase microextraction gas chromatography–mass spectrometry (SPME/GC-MS) method which, as an outcome, gives a better-defined quantification of the adsorption properties on NPs. We investigated the various aspects of the SPME/GC-MS method, including kinetics of adsorption of probe compounds on SPME fiber, kinetic of adsorption of probe compounds on NP's surface, and optimization of NP's concentration. The optimized conditions were then tested on 33 probe compounds and on Au NPs (15 nm) and SiO(2) NPs (50 nm). The procedure allowed the identification of three compounds adsorbed by silica NPs and nine compounds by Au NPs, with equilibrium times which varied between 30 min and 12 h. Adsorption coefficients of 4.66 ± 0.23 and 4.44 ± 0.26 were calculated for 1-methylnaphtalene and biphenyl, compared to literature values of 4.89 and 5.18, respectively. The results demonstrated that the detailed optimization of the SPME/GC-MS method under various conditions is a critical factor and a prerequisite to the application of the BSAI approach as a tool to characterize surface adsorption properties of NPs and therefore to draw any further conclusions on their potential impact on health. [Figure: see text