Tripartite phase separation of two signal effectors with vesicles priming B cell responsiveness.

Antibody-mediated immune responses rely on antigen recognition by the B cell antigen receptor (BCR) and the proper engagement of its intracellular signal effector proteins. Src homology (SH) 2 domain-containing leukocyte protein of 65 kDa (SLP65) is the key scaffold protein mediating BCR signaling. In resting B cells, SLP65 colocalizes with Cbl-interacting protein of 85 kDa (CIN85) in cytoplasmic granules whose formation is not fully understood. Here we show that effective B cell activation requires tripartite phase separation of SLP65, CIN85, and lipid vesicles into droplets via vesicle binding of SLP65 and promiscuous interactions between nine SH3 domains of the trimeric CIN85 and the proline-rich motifs (PRMs) of SLP65. Vesicles are clustered and the dynamical structure of SLP65 persists in the droplet phase in vitro. Our results demonstrate that phase separation driven by concerted transient interactions between scaffold proteins and vesicles is a cellular mechanism to concentrate and organize signal transducers

A reduced-reference perceptual image and video quality metric based on edge preservation

In image and video compression and transmission, it is important to rely on an objective image/video quality metric which accurately represents the subjective quality of processed images and video sequences. In some scenarios, it is also important to evaluate the quality of the received video sequence with minimal reference to the transmitted one. For instance, for quality improvement of video transmission through closed-loop optimisation, the video quality measure can be evaluated at the receiver and provided as feedback information to the system controller. The original image/video sequence-prior to compression and transmission-is not usually available at the receiver side, and it is important to rely at the receiver side on an objective video quality metric that does not need reference or needs minimal reference to the original video sequence. The observation that the human eye is very sensitive to edge and contour information of an image underpins the proposal of our reduced reference (RR) quality metric, which compares edge information between the distorted and the original image. Results highlight that the metric correlates well with subjective observations, also in comparison with commonly used full-reference metrics and with a state-of-the-art RR metric. © 2012 Martini et al

Directed invasion of cancer cell spheroids inside 3D collagen matrices oriented by microfluidic flow in experiment and simulation

Invasion is strongly influenced by the mechanical properties of the extracellular matrix. Here, we use microfluidics to align fibers of a collagen matrix and study the influence of fiber orientation on invasion from a cancer cell spheroid. The microfluidic setup allows for highly oriented collagen fibers of tangential and radial orientation with respect to the spheroid, which can be described by finite element simulations. In invasion experiments, we observe a strong bias of invasion towards radial as compared to tangential fiber orientation. Simulations of the invasive behavior with a Brownian diffusion model suggest complete blockage of migration perpendicularly to fibers allowing for migration exclusively along fibers. This slows invasion toward areas with tangentially oriented fibers down, but does not prevent it

Intra-oral compartment pressures: a biofunctional model and experimental measurements under different conditions of posture

Oral posture is considered to have a major influence on the development and reoccurrence of malocclusion. A biofunctional model was tested with the null hypotheses that (1) there are no significant differences between pressures during different oral functions and (2) between pressure measurements in different oral compartments in order to substantiate various postural conditions at rest by intra-oral pressure dynamics. Atmospheric pressure monitoring was simultaneously carried out with a digital manometer in the vestibular inter-occlusal space (IOS) and at the palatal vault (sub-palatal space, SPS). Twenty subjects with normal occlusion were evaluated during the open-mouth condition (OC), gently closed lips (semi-open compartment condition, SC), with closed compartments after the generation of a negative pressure (CCN) and swallowing (SW). Pressure curve characteristics were compared between the different measurement phases (OC, SC, CCN, SW) as well as between the two compartments (IOS, SPS) using analysis of variance and Wilcoxon matched-pairs tests adopting a significance level of α = 0.05. Both null hypotheses were rejected. Average pressures (IOS, SPS) in the experimental phases were 0.0, −0.08 (OC); −0.16, −1.0 (SC); −48.79, −81.86 (CCN); and −29.25, −62.51 (SW) mbar. CCN plateau and peak characteristics significantly differed between the two compartments SPS and IOS. These results indicate the formation of two different intra-oral functional anatomical compartments which provide a deeper understanding of orofacial biofunctions and explain previous observations of negative intra-oral pressures at rest

Generalized Pickands constants and stationary max-stable processes

Pickands constants play a crucial role in the asymptotic theory of Gaussian processes. They are commonly defined as the limits of a sequence of expectations involving fractional Brownian motions and, as such, their exact value is often unknown. Recently, Dieker and Yakir (Bernoulli, 20(3), 1600–1619, 2014) derived a novel representation of Pickands constant as a simple expected value that does not involve a limit operation. In this paper we show that the notion of Pickands constants and their corresponding Dieker–Yakir representations can be extended to a large class of stochastic processes, including general Gaussian and Lévy processes. We furthermore develop a link to extreme value theory and show that Pickands-type constants coincide with certain constants arising in the study of max-stable processes with mixed moving maxima representations

Extremal behavior of squared Bessel processes attracted by the Brown-Resnick process

The convergence of properly time-scaled and normalized maxima of independent standard Brownian motions to the Brown-Resnick process is well-known in the literature. In this paper, we study the extremal functional behavior of non-Gaussian processes, namely squared Bessel processes and scalar products of Brownian motions. It is shown that maxima of independent samples of those processes converge weakly on the space of continuous functions to the Brown-Resnick process

Psychophysiology-based QoE assessment : a survey

We present a survey of psychophysiology-based assessment for quality of experience (QoE) in advanced multimedia technologies. We provide a classification of methods relevant to QoE and describe related psychological processes, experimental design considerations, and signal analysis techniques. We summarize multimodal techniques and discuss several important aspects of psychophysiology-based QoE assessment, including the synergies with psychophysical assessment and the need for standardized experimental design. This survey is not considered to be exhaustive but serves as a guideline for those interested to further explore this emerging field of research

Conditional Sampling for Max-Stable Processes with a Mixed Moving Maxima Representation

This paper deals with the question of conditional sampling and prediction for the class of stationary max-stable processes which allow for a mixed moving maxima representation. We develop an exact procedure for conditional sampling using the Poisson point process structure of such processes. For explicit calculations we restrict ourselves to the one-dimensional case and use a finite number of shape functions satisfying some regularity conditions. For more general shape functions approximation techniques are presented. Our algorithm is applied to the Smith process and the Brown-Resnick process. Finally, we compare our computational results to other approaches. Here, the algorithm for Gaussian processes with transformed marginals turns out to be surprisingly competitive.Comment: 35 pages; version accepted for publication in Extremes. The final publication is available at http://link.springer.co

Absolute photometric calibration of IRAC: lessons learned using nine years of flight data

Significant improvements in our understanding of various photometric effects have occurred in the more than nine years of flight operations of the Infrared Array Camera aboard the Spitzer Space Telescope. With the accumulation of calibration data, photometric variations that are intrinsic to the instrument can now be mapped with high fidelity. Using all existing data on calibration stars, the array location-dependent photometric correction (the variation of flux with position on the array) and the correction for intra-pixel sensitivity variation (pixel-phase) have been modeled simultaneously. Examination of the warm mission data enabled the characterization of the underlying form of the pixelphase variation in cryogenic data. In addition to the accumulation of calibration data, significant improvements in the calibration of the truth spectra of the calibrators has taken place. Using the work of Engelke et al. (2006), the KIII calibrators have no offset as compared to the AV calibrators, providing a second pillar of the calibration scheme. The current cryogenic calibration is better than 3% in an absolute sense, with most of the uncertainty still in the knowledge of the true flux densities of the primary calibrators. We present the final state of the cryogenic IRAC calibration and a comparison of the IRAC calibration to an independent calibration methodology using the HST primary calibrators