Estimation of woody plant species diversity during a dry season in a savanna environment using the spectral and textural information derived from WorldView-2 imagery

Abstract: Remote sensing techniques are useful in the monitoring of woody plant species diversity in different environments including in savanna vegetation types. However, the performance of satellite imagery in assessing woody plant species diversity in dry seasons has been understudied. This study aimed to assess the performance of multiple Gray Level Co-occurrence Matrices (GLCM) derived from individual bands of WorldView-2 satellite imagery to quantify woody plant species diversity in a savanna environment during the dry season. Woody plant species were counted in 220 plots (20 m radius) and subsequently converted to a continuous scale of the Shannon species diversity index. The index regressed against the GLCMs using the all-possible-subsets regression approach that builds competing models to choose from. Entropy GLCM yielded the best overall accuracy (adjusted R2: 0.41−0.46; Root Mean Square Error (RMSE): 0.60−0.58) in estimating species diversity. The effect of the number of predicting bands on species diversity estimation was also explored. Accuracy generally increased when three–five bands were used in models but stabilised or gradually decreased as more than five bands were used. Despite the peak accuracies achieved with three–five bands, performances still fared well for models that used fewer bands, showing the relevance of few bands for species diversity estimation. We also assessed the effect of GLCM window size (3×3, 5×5 and 7×7) on species diversity estimation and generally found inconsistent conclusions. These findings demonstrate the capability of GLCMs combined with high spatial resolution imagery in estimating woody plants species diversity in a savanna environment during the dry period. It is important to test the performance of species diversity estimation of similar environmental set-ups using widely available moderate-resolution imagery

Integrated Multi-Satellite Evaluation for the Global Precipitation Measurement: Impact of Precipitation Types on Spaceborne Precipitation Estimation

Integrated multi-sensor assessment is proposed as a novel approach to advance satellite precipitation validation in order to provide users and algorithm developers with an assessment adequately coping with the varying performances of merged satellite precipitation estimates. Gridded precipitation rates retrieved from space sensors with quasi-global coverage feed numerous applications ranging from water budget studies to forecasting natural hazards caused by extreme events. Characterizing the error structure of satellite precipitation products is recognized as a major issue for the usefulness of these estimates. The Global Precipitation Measurement (GPM) mission aims at unifying precipitation measurements from a constellation of low-earth orbiting (LEO) sensors with various capabilities to detect, classify and quantify precipitation. They are used in combination with geostationary observations to provide gridded precipitation accumulations. The GPM Core Observatory satellite serves as a calibration reference for consistent precipitation retrieval algorithms across the constellation. The propagation of QPE uncertainty from LEO active/passive microwave (PMW) precipitation estimates to gridded QPE is addressed in this study, by focusing on the impact of precipitation typology on QPE from the Level-2 GPM Core Observatory Dual-frequency Precipitation Radar (DPR) to the Microwave Imager (GMI) to Level-3 IMERG precipitation over the Conterminous U.S. A high-resolution surface precipitation used as a consistent reference across scales is derived from the ground radar-based Multi-Radar/Multi-Sensor. While the error structure of the DPR, GMI and subsequent IMERG is complex because of the interaction of various error factors, systematic biases related to precipitation typology are consistently quantified across products. These biases display similar features across Level-2 and Level-3, highlighting the need to better resolve precipitation typology from space and the room for improvement in global-scale precipitation estimates. The integrated analysis and framework proposed herein applies more generally to precipitation estimates from sensors and error sources affecting low-earth orbiting satellites and derived gridded products

The impact of rotavirus gastroenteritis on the family

BACKGROUND: Rotavirus is the leading cause of severe diarrhea in young children and causes substantial morbidity and mortality. Although the clinical aspects have been well described, little information is available regarding the emotional, social, and economic impact of rotavirus gastroenteritis on the family of a sick child. The objectives of this study were to: 1) assess the family impact of rotavirus gastroenteritis through qualitative interviews with parents; 2) compare the clinical severity of rotavirus-positive and negative gastroenteritis; 3) test a questionnaire asking parents to rank the importance of various factors associated with a case of rotavirus gastroenteritis. METHODS: The study enrolled parents and children (2–36 months of age) brought to one of the study sites (outpatient clinic or ER) if the child experienced ≥ 3 watery or looser-than normal stools and/or forceful vomiting within any 24-hour period within the prior 3 days. The clinical severity of each child's illness was rated using a clinical scoring system and stool samples were tested for rotavirus antigen. Parents of rotavirus-positive children were invited to participate in focus group or individual interviews and subsequently completed a questionnaire regarding the impact of their child's illness. RESULTS: Of 62 enrolled children, 43 stool samples were collected and 63% tested positive for rotavirus. Illness was more severe in children with rotavirus-positive compared to rotavirus-negative gastroenteritis (92% vs. 37.5% rated as moderate/severe). Seventeen parents of rotavirus-positive children participated in the interviews and completed the written questionnaire. Parents were frightened by the severity of vomiting and diarrhea associated with rotavirus gastroenteritis, and noted that family life was impacted in several ways including loss of sleep, missed work, and an inability to complete normal household tasks. They expressed frustration at the lack of a specific medication and the difficulty of treating the illness with oral rehydration solutions, but had a largely positive outlook concerning the prospect of a rotavirus vaccine. CONCLUSION: A better understanding of how rotavirus gastroenteritis impacts the family can help healthcare providers ease parental fears and advise them on the characteristics of this illness, practices to prevent infection, and the optimal care of an affected child

2009 H1N1 and Seasonal Influenza Immunization Among Pregnant Women: a Comparison of Different Sources of Immunization Information

Validity of prenatal immunization data from different sources has not been assessed. We evaluated prenatal 2009 H1N1 and seasonal influenza (FLU) data obtained from state immunization information systems (IIS), medical record abstraction (MRA), and participant recall using medical care logs (NCS-MCL). 2009 H1N1 and FLU data were obtained from IIS and MRA for 325 pregnant women participating in the National Children\u27s Study at three locations (SD/MN, NC, WI). Women recalled immunizations at first pregnancy visit and at 16-17 and 36 weeks\u27 gestation (NCS-MCL). The proportion of women with vaccine information obtainable from each data source was determined, and proportions immunized as determined using different data sources were compared. IIS data were available for 82%, MRA for 97%, and NCS-MCL for 93% of women. No mention of either vaccine occurred in 29% (range 4-48%) of IIS, 40% of MRA (25-59%), and 59% (43-82%) in NCS-MCL. Best agreement between sources was 2009 H1N1 vaccine in MRA versus IIS [kappa (95% CI) of 0.44 (0.32-0.55)], with poorest agreement for FLU in IIS versus NCS-MCL [0.11 (-0.03 to 0.25)]. IIS was the most sensitive method for identifying women receiving 2009 H1N1 vaccine (92%); MRA was most sensitive for FLU vaccine (81%). IIS provided the most complete and sensitive data for 2009 H1N1 immunizations and MRA the most complete and sensitive data for FLU; IIS data were available for a smaller percent of population than MRA. NCS-MCL was the least sensitive method for identifying vaccinated women

Parents' reported preference scores for childhood atopic dermatitis disease states

BACKGROUND: We sought to elicit preference weights from parents for health states corresponding to children with various levels of severity of atopic dermatitis. We also evaluated the hypothesis that parents with children who had been diagnosed with atopic dermatitis would assign different preferences to the health state scenarios compared with parents who did not have a child with atopic dermatitis. METHODS: Subjects were parents of children aged 3 months to 18 years. The sample was derived from the General Panel, Mommies Sub-Panel, and Chronic Illness Sub-Panel of Harris Interactive. Participants rated health scenarios for atopic dermatitis, asthma, and eyeglasses on a visual analog scale, imagining a child was experiencing the described state. RESULTS: A total of 3539 parents completed the survey. Twenty-nine percent had a child with a history of atopic dermatitis. Mean preference scores for atopic dermatitis were as follows: mild, 91 (95% confidence interval [CI], 90.7 to 91.5); mild/moderate, 84 (95%CI, 83.5 to 84.4); moderate, 73 (95%CI, 72.5 to 73.6); moderate/severe, 61 (95%CI, 60.6 to 61.8); severe, 49 (95% CI, 48.7 to 50.1); asthma, 58 (95%CI, 57.4 to 58.8); and eyeglasses, 87(95%CI, 86.3 to 87.4). CONCLUSIONS: Parents perceive that atopic dermatitis has a negative effect on quality of life that increases with disease severity. Estimates of parents' preferences can provide physicians with insight into the value that parents place on their children's treatment and can be used to evaluate new medical therapies for atopic dermatitis

State-Level Immunization Information Systems: Potential for Childhood Immunization Data Linkages.

Objectives Sources of immunization data include state registries or immunization information systems (IIS), medical records, and surveys. Little is known about the quality of these data sources or the feasibility of using IIS data for research. We assessed the feasibility of collecting immunization information for a national children\u27s health study by accessing existing IIS data and comparing the completeness of these data against medical record abstractions (MRA) and parent report. Staff time needed to obtain IIS and MRA data was assessed. Methods We administered a questionnaire to state-level IIS representatives to ascertain availability and completeness of their data for research and gather information about data formats. We evaluated quality of data from IIS, medical records, and reports from parents of 119 National Children\u27s Study participants at three locations. Results IIS data were comparable to MRA data and both were more complete than parental report. Agreement between IIS and MRA data was greater than between parental report and MRA, suggesting IIS and MRA are better sources than parental report. Obtaining IIS data took less staff time than chart review, making IIS data linkage for research a preferred choice. Conclusions IIS survey results indicate data can be obtained by researchers using data linkages. IIS are an accessible and feasible child immunization information source and these registries reduce reliance on parental report or medical record abstraction. Researchers seeking to link IIS data with large multi-site studies should consider acquiring IIS data, but may need strategies to overcome barriers to data completeness and linkage

Consumption and acceptability of whole grain staples for lowering markers of diabetes risk among overweight and obese Tanzanian adults

Background: Dietary changes characterized by a reduction in carbohydrate quality are occurring in developing countries and may be associated with a higher prevalence of obesity and chronic diseases such as type 2 diabetes mellitus. We assessed the preferences and acceptability of unrefined whole grain carbohydrate staples (i.e., brown rice, unrefined maize and unrefined sorghum ugali) as substitutes for commonly consumed refined carbohydrates in Tanzania. Methods: A questionnaire was used to collect sociodemographic information and dietary habits, and pre-and post-tasting questionnaires were administered for test foods. A 10-point LIKERT scale was used to rate attributes of the three test foods. Results: White rice and refined maize ugali were the most commonly consumed carbohydrate staples in this population; 98% and 91%, respectively. Occasional consumption of unrefined maize and sorghum ugali was reported by 32% and 23% of the participants, respectively. All of the test foods were highly rated for smell, taste, color, appearance and texture. Taste was rated highest for unrefined maize ugali. Almost all of the participants were willing to participate in a future dietary intervention involving regular consumption of these unrefined carbohydrates for at least six months duration. Conclusions: These findings suggest that whole grain carbohydrates are highly acceptable, and that there is a promising potential for their use in future dietary intervention studies in Tanzania

Lp-PLA2 Activity During Iron Depletion Treatment in Primary IO Patients

Background: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an inflammatory biomarker involved in atherosclerosis and cardiovascular disease (CVD). Iron stores may modify Lp-PLA2 as higher activity levels wereobserved in patients with primary iron overload (IO).Aim: to evaluate the changes of Lp-PLA2 activity and other atherosclerosis markers in patients with primary IO after iron depletion.Materials and Methods:The study initially included 20 male patients with primary IO, defined by liver histology,from which 7 were lost during follow-up and 13 completed the study (mean follow-up duration: 24±6 months).Phlebotomy treatment consisted in the removal of 1 unit of blood weekly or biweekly. We recorded traditional cardiovascular risk factors, high sensitivity C-reactive protein (hsCRP), and Lp-PLA2 activity. Longitudinal differences were tested by paired T or Wilcoxon tests. Linear regression was used to evaluate the relationship between changes in ferritin and in Lp-PLA2.Results: HFE mutations were present in 77% of the patients. Besides ferritin concentration (-74%), ALT (-11%) and Lp-PLA2 activities (-14%) were reduced after iron depletion (all p<0.05). Linear regression showed that changes in ferritin levels explained a 60% of the variability in the changes of Lp-PLA2 activity (B=0.80, p=0.008, R2 = 0.60).Conclusions: Treatment by phlebotomy significantly reduced the levels of Lp-PLA2 activity besides its expected effects in liver markers. The implications of iron depletion for the reduction of CVD risk remain to be studied.Fil: Tetzlaff, Walter Francisco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Martín, Maximiliano Emmanuel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Botta, Eliana Elizabeth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Saez, María Soledad. Hospital Italiano; ArgentinaFil: Ferraro, Maria Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Boero, Laura Estela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Sorroche, Patricia B. Hospital Italiano; ArgentinaFil: Arbelbide, Jorge. Hospital Italiano; ArgentinaFil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Meroño, Tomás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentin

Lp-PLA2 Activity During Iron Depletion Treatment in Primary IO Patients

Background: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an inflammatory biomarker involved in atherosclerosis and cardiovascular disease (CVD). Iron stores may modify Lp-PLA2 as higher activity levels wereobserved in patients with primary iron overload (IO).Aim: to evaluate the changes of Lp-PLA2 activity and other atherosclerosis markers in patients with primary IO after iron depletion.Materials and Methods:The study initially included 20 male patients with primary IO, defined by liver histology,from which 7 were lost during follow-up and 13 completed the study (mean follow-up duration: 24±6 months).Phlebotomy treatment consisted in the removal of 1 unit of blood weekly or biweekly. We recorded traditional cardiovascular risk factors, high sensitivity C-reactive protein (hsCRP), and Lp-PLA2 activity. Longitudinal differences were tested by paired T or Wilcoxon tests. Linear regression was used to evaluate the relationship between changes in ferritin and in Lp-PLA2.Results: HFE mutations were present in 77% of the patients. Besides ferritin concentration (-74%), ALT (-11%) and Lp-PLA2 activities (-14%) were reduced after iron depletion (all p<0.05). Linear regression showed that changes in ferritin levels explained a 60% of the variability in the changes of Lp-PLA2 activity (B=0.80, p=0.008, R2 = 0.60).Conclusions: Treatment by phlebotomy significantly reduced the levels of Lp-PLA2 activity besides its expected effects in liver markers. The implications of iron depletion for the reduction of CVD risk remain to be studied.Fil: Tetzlaff, Walter Francisco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Martín, Maximiliano Emmanuel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Botta, Eliana Elizabeth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Saez, María Soledad. Hospital Italiano; ArgentinaFil: Ferraro, Maria Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Boero, Laura Estela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Sorroche, Patricia B. Hospital Italiano; ArgentinaFil: Arbelbide, Jorge. Hospital Italiano; ArgentinaFil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Meroño, Tomás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentin