Maslinic acid alleviates ischemia/reperfusion-induced inflammation by downregulation of NFκB-mediated adhesion molecule expression

Ischemia/reperfusion (I/R)-induced inflammation is associated with enhanced leukocyte rolling, adhesion and transmigration within the microcirculation. These steps are mediated by hypoxia-triggered signaling pathways, which upregulate adhesion molecule expression on endothelial cells and pericytes. We analyzed whether these cellular events are affected by maslinic acid (MA). Mitochondrial activity and viability of MA-exposed endothelial cells and pericytes were assessed by water-soluble tetrazolium (WST)-1 and lactate dehydrogenase (LDH) assays as well as Annexin V/propidium iodide (PI) stainings. Effects of MA on hypoxia and reoxygenation-induced expression of E-selectin, intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 were determined by flow cytometry. The subcellular localization of the NFκB subunit p65 was analyzed by immunofluorescence and Western blot. I/R-induced leukocytic inflammation was studied in MA- and vehicle-treated mouse dorsal skinfold chambers by intravital fluorescence microscopy and immunohistochemistry. MA did not affect viability, but suppressed the mitochondrial activity of endothelial cells. Furthermore, MA reduced adhesion molecule expression on endothelial cells and pericytes due to an inhibitory action on NFκB signaling. Numbers of adherent and transmigrated leukocytes were lower in post-ischemic tissue of MA-treated mice when compared to vehicle-treated controls. In addition, MA affected reactive oxygen species (ROS) formation, resulting in a diminished oxidative DNA damage. Hence, MA represents an attractive compound for the establishment of novel therapeutic approaches against I/R-induced inflammation

Chemical expansion of La0.8Sr0.2Fe0.7Ga0.3O3-δ

International audienceThis paper deals with the chemical expansion measurements and modelling of La0.8Sr0.2Fe0.7Ga0.3O3-δ. The expansion behavior has been evaluated using a dilatometer and X-ray diffraction over a wide range of temperatures (RT to 1373 K) and oxygen partial pressures (10−21 to 1 atm). The material stoichiometry evolution with temperature and oxygen partial pressure has been measured using thermogravimetry analysis at different oxygen partial pressure, from 10−21 to 0.5 atm and from RT to 1473 K. Considering a typical defect model for lanthanum ferrite oxides, chemical expansion depends linearly on the Fe4+ concentration rather than on the oxygen vacancy concentration. A model of chemical expansion as a function of pO2 and temperature is then proposed. It helps to understand and anticipate the chemical expansion behavior exhibited by this material when used as Ionic Transport Membrane (ITM)

Deep‐water sediment transport patterns and basin floor topography in early rift basins: Plio‐Pleistocene syn‐rift of the Corinth Rift, Greece

Our current understanding on sedimentary deep‐water environments is mainly built of information obtained from tectonic settings such as passive margins and foreland basins. More observations from extensional settings are particularly needed in order to better constrain the role of active tectonics in controlling sediment pathways, depositional style and stratigraphic stacking patterns. This study focuses on the evolution of a Plio‐Pleistocene deep‐water sedimentary system (Rethi‐Dendro Formation) and its relation to structural activity in the Amphithea fault block in the Corinth Rift, Greece. The Corinth Rift is an active extensional basin in the early stages of rift evolution, providing perfect opportunities for the study of early deep‐water syn‐rift deposits that are usually eroded from the rift shoulders due to erosion in mature basins like the Red Sea, North Sea and the Atlantic rifted margin. The depocentre is located at the exit of a structurally controlled sediment fairway, approximately 15 km from its main sediment source and 12 km basinwards from the basin margin coastline. Fieldwork, augmented by digital outcrop techniques (LiDAR and photogrammetry) and clast‐count compositional analysis allowed identification of 16 stratigraphic units that are grouped into six types of depositional elements: A—mudstone‐dominated sheets, B—conglomerate‐dominated lobes, C—conglomerate channel belts and sandstone sheets, D—sandstone channel belts, E—sandstone‐dominated broad shallow lobes, F—sandstone‐dominated sheets with broad shallow channels. The formation represents an axial system sourced by a hinterland‐fed Mavro delta, with minor contributions from a transverse system of conglomerate‐dominated lobes sourced from intrabasinal highs. The results of clast compositional analysis enable precise attribution for the different sediment sources to the deep‐water system and their link to other stratigraphic units in the area. Structures in the Amphithea fault block played a major role in controlling the location and orientation of sedimentary systems by modifying basin‐floor gradients due to a combination of hangingwall tilt, displacement of faults internal to the depocentre and folding on top of blind growing faults. Fault activity also promoted large‐scale subaqueous landslides and eventual uplift of the whole fault block

Organic Wheat Farming Improves Grain Zinc Concentration

Zinc (Zn) nutrition is of key relevance in India, as a large fraction of the population suffers from Zn malnutrition and many soils contain little plant available Zn. In this study we compared organic and conventional wheat cropping systems with respect to DTPA (diethylene triamine pentaacetic acid)-extractable Zn as a proxy for plant available Zn, yield, and grain Zn concentration. We analyzed soil and wheat grain samples from 30 organic and 30 conventional farms in Madhya Pradesh (central India), and conducted farmer interviews to elucidate sociological and management variables. Total and DTPA-extractable soil Zn concentrations and grain yield (3400 kg ha-1) did not differ between the two farming systems, but with 32 and 28 mg kg-1 respectively, grain Zn concentrations were higher on organic than conventional farms (t = -2.2, p = 0.03). Furthermore, multiple linear regression analyses revealed that (a) total soil zinc and sulfur concentrations were the best predictors of DTPA-extractable soil Zn, (b) Olsen phosphate taken as a proxy for available soil phosphorus, exchangeable soil potassium, harvest date, training of farmers in nutrient management, and soil silt content were the best predictors of yield, and (c) yield, Olsen phosphate, grain nitrogen, farmyard manure availability, and the type of cropping system were the best predictors of grain Zn concentration. Results suggested that organic wheat contained more Zn despite same yield level due to higher nutrient efficiency. Higher nutrient efficiency was also seen in organic wheat for P, N and S. The study thus suggests that appropriate farm management can lead to competitive yield and improved Zn concentration in wheat grains on organic farms

New Enabling Technologies to Observe and Characterise Urban Environments with Big Data from Space – the Urban Thematic Exploitation Platform

Modern Earth Observation (EO) satellite missions provide valuable opportunities to support sustainable urban planning and management by delivering dedicated information on the spatiotemporal development of the built environment and its key morphological and physical characteristics such as imperviousness, greenness, built-up density, building volume, albedo – from global down to local scale. However, the transformation of the raw EO imagery into ready-to-use thematic data and indicators for scientist or planners on the one hand and actionable information for decision makers on the other hand requires detailed technical expert knowledge. Moreover, the imagery collected by satellite missions such as the US Landsat program or the European fleet of Sentinel satellites, but also by airborne systems or drones, rapidly adds up to a multiple of the data volume that can effectively be handled with standard work stations and software solutions. Hence, this contribution introduces the Urban Thematic Exploitation Platform (https://urban-tep.eo.esa.int) that utilizes modern information and communication technology to bridge the gap between the mass data collections of the technology-driven EO sector and the demand of science, planning, and policy for up-to-date information on the status, properties and dynamics of the urban system. Key components of the Urban Thematic Exploitation Platform (U-TEP) are an open, web-based portal that is connected to distributed high-level computing clusters and clouds and that also provides key functionalities for i) high-performance data access, analysis and visualization, ii) customized development and sharing of algorithms, products and services, and iii) networking, communication and exchange of data and information. The overarching objective here is to enable any interested (non-expert) user to easily generate actionable indicators and information for effective sustainable urban development based on a joint analysis of various data sources such as official survey data, EO mission data, socio-economic statistics, and data collected via social media or citizen science. So far more than 3.5 PB of data have been processed and analyzed by means of the U-TEP to finally provide a broad spectrum of urban information products and related services for visualization and analytics that have yet successfully been used by more than 240 institutions (science, planning, NGOs, policy) from 41 countries (i.a. World Bank Group, United Nations, Organisation for Economic Cooperation and Development, World Food Programme, Bill and Melinda Gates Foundation, Group on Earth Observation, Global Platform for Sustainable Cities)

Proposed treatment strategy for reactive hypoglycaemia

Background/aim: Managing reactive hypoglycaemia (RH) poses challenges due to limited and often ineffective treatment options. We report a case series and draw on this to propose a stepwise treatment approach consisting of lifestyle modifications, metformin, GLP-1 analogues, and the use of flash glucose monitoring technology. Method: A retrospective review was conducted to analyse the management of 11 cases presenting with recurrent RH symptoms. Result: Two patients experienced successful resolution of symptoms through lifestyle modifications. Metformin alone was effective in treating seven out of nine patients who received pharmacological treatment. Two patients with previous upper gastrointestinal surgery showed a partial response to metformin and benefited further from additional long-acting GLP-1 analogue. Pharmacological intervention led to significant reductions in insulin and C-peptide levels in repeat mixed meal tolerance tests (P-values 0.043 for insulin and 0.006 for C-peptide). Finally, flash glucose monitoring technology was useful in early detection and preventing episodes of hypoglycaemia in one of these patients with persistent symptoms. Conclusion: These findings highlight the potential efficacy of escalated treatment strategies for RH, including the use of metformin, GLP-1 analogues, and flash glucose monitoring technology

Differential acylated ghrelin, peptide YY3-36, appetite, and food intake responses to equivalent energy deficits created by exercise and food restriction

Context: Acute energy deficits imposed by food restriction increase appetite and energy intake; however, these outcomes remain unchanged when energy deficits are imposed by exercise.Objective: Our objective was to determine the potential role of acylated ghrelin and peptide YY3-36 (PYY3-36) in mediating appetite and energy intake responses to identical energy deficits imposed by food restriction and exercise.Design: Twelve healthy males completed three 9-h trials (exercise deficit, food deficit, and control) in a randomized counterbalanced design. Participants ran for 90 min (70% of VO2 max) at the beginning of the exercise deficit trial and then rested for 7.5 h. Participants remained sedentary throughout the food deficit and control trials. Test meals were consumed by participants at 2 and 4.75 h in all trials. The amount provided in the food deficit trial was restricted so that an energy deficit (equivalent to that imposed by exercise) was induced relative to control. Participants were permitted access to a buffet meal at 8 h.Results: The energy deficits imposed by food restriction (4820 +/- 151 kJ) and exercise (4715 +/- 113 kJ) were similar. Appetite and ad libitum energy intake responded in a compensatory fashion to food restriction yet were not influenced by exercise. Plasma acylated ghrelin concentrations increased, whereas PYY3-36 decreased, in response to food restriction (two-way ANOVA, trial x time interaction, P < 0.001 for each). Exercise did not induce such compensatory responses.Conclusions: These findings suggest a mediating role of acylated ghrelin and PYY3-36 in determining divergent feeding responses to energy deficits imposed by food restriction and exercise. (J Clin Endocrinol Metab 96: 1114-1121, 2011