3,459 research outputs found
Katniss as a Representation of Girl Power in Hunger Games Trilogy
This study is about the representation of girl power in Hunger Games trilogy.Hunger Games trilogy shows how women that are represented by the main character “Katniss”, can do what they want regardless others people opinion. It shows that Katniss as the main character lives in a society that does not have roles between man and woman by showing some action through her characterisctics in the story that against the culture and society. She is able to do anything without thinking about what man or woman should do. Thus,this thesis aims at showing girl power characteristics that are being represented by Katniss. Therefore, I will analyze girl power characteristics in Katniss by using girl power concept. Moreover through this analysis, I find that there are some girl power characteristics that are being represented by Katniss. First, she is an independent woman. Second, she is equally depends on her “brains and brawn”. Third, Katniss is a self-determined woman. For the result of the analysis, it can be concluded that Katniss is the representation of girl power that is shown through her action, words, and thoughts
Action of HMGB1 on miR221/222 cluster in neuroblastoma cell lines
microRNA (miR/miRNA) are small non-coding RNAs that control gene expression at the post-transcriptional level by targeting mRNAs. Aberrant expression of miRNAs is often observed in different types of cancer. Specific miRNAs function as tumor suppressors or oncogenes and interfere with various aspects of carcinogenesis, including differentiation, proliferation and invasion. Upregulation of miRNAs 221 and 222 has been shown to induce a malignant phenotype in numerous human cancers via inhibition of phosphatase and tensin homolog (PTEN) expression. Neuroblastoma is the most common extracranial solid malignancy in children, which is characterized by cellular heterogeneity that corresponds to different clinical outcomes. The different cellular phenotypes are associated with different gene mutations and miRs that control genetic and epigenetic factors. For this reason miRs are considered a potential therapeutic target in neuroblastoma. The aim of the present study was to investigate the mechanisms by which extracellular high mobility group box 1 (HMGB1) promotes cell growth in neuroblastoma. SK-N-BE(2) and SH-SY5Y neuroblastoma derived cell lines were transfected with the antisense oligonucleotides, anti-miR-221 and -222, followed by treatment with HMGB1 to investigate the expression of the oncosuppressor PTEN. In this study, it was demonstrated that HMGB1, which is released by damaged cells and tumor cells, upregulates miR-221/222 oncogenic clusters in the two human neuroblastoma derived cell lines. The results revealed that the oncogenic cluster miRs 221/222 were more highly expressed by the most undifferentiated cell line [SK-N-BE(2)] compared with the the less tumorigenic cell line (SH-SY5Y) and that exogenous HMGB1 increases this expression. In addition, HMGB1 modulates PTEN expression via miR-221/222, as demonstrated by transiently blocking miR-221/222 with anti-sense oligonucleotides. These results may lead to the development of novel therapeutic strategies for neuroblastoma
Extrapolation in games of coordination and dominance solvable games
We study extrapolation between games in a laboratory experiment. Participants in our experiment first play either the dominance solvable guessing game or a Coordination version of the guessing game for five rounds. Afterwards they play a 3x3 normal form game for ten rounds with random matching which is either a game solvable through iterated elimination of dominated strategies (IEDS), a pure Coordination game or a Coordination game with pareto ranked equilibria. We find strong evidence that participants do extrapolate between games. Playing a strategically different game hurts compared to the control treatment where no guessing game is played before and in fact impedes convergence to Nash equilibrium in both the 3x3 IEDS and the Coordination games. Playing a strategically similar game before leads to faster convergence to Nash equilibrium in the second game. In the Coordination games some participants try to use the first game as a Coordination device. Our design and results allow us to conclude that participants do not only learn about the population and/or succesful actions, but that they are also able to learn structural properties of the games
HMGB1-Induced Cross Talk between PTEN and miRs 221/222 in Thyroid Cancer
High mobility group box 1 (HMGB1) is an ubiquitous protein that plays different roles in the nucleus, cytoplasm and extra-cellular space. It is an important DAMP molecule that allows communication between damaged or tumor cells and the immune system. Tumor cells exploit HMGB1’s ability to activate intracellular pathways that lead to cell growth and migration. Papillary thyroid cancer is a well differentiated tumor and is often used to study relationships between cells and the inflammatory microenvironment as the latter is characterized by high levels of inflammatory cells and cytokines. Anaplastic thyroid cancer is one of the most lethal human cancers in which many microRNAs and tumor suppressor genes are de-regulated.
Up-regulation of microRNAs 221 and 222 has been shown to induce the malignant phenotype in many human cancers via inhibition of PTEN expression.
In this study we suggest that extracellular HMGB1 interaction with RAGE enhances expression of oncogenic cluster miR221/222 that in turn inhibits tumor suppressor gene PTEN in two cell lines derived from human thyroid anaplastic and papillary cancers.
The newly identified pathway HMGB1/RAGE/miR 221/222 may represent an effective way of tumor escape from immune surveillance that could be used to develop new therapeutic strategies against anaplastic tumors
A spherical model with directional interactions: I. Static properties
We introduce a simple spherical model whose structural properties are similar
to the ones generated by models with directional interactions, by employing a
binary mixture of large and small hard spheres, with a square-well attraction
acting only between particles of different size. The small particles provide
the bonds between the large ones. With a proper choice of the interaction
parameters, as well as of the relative concentration of the two species, it is
possible to control the effective valence. Here we focus on a specific choice
of the parameters which favors tetrahedral ordering and study the equilibrium
static properties of the system in a large window of densities and
temperatures. Upon lowering the temperature we observe a progressive increase
in local order, accompanied by the formation of a four-coordinated network of
bonds. Three different density regions are observed: at low density the system
phase separates into a gas and a liquid phase; at intermediate densities a
network of fully bonded particles develops; at high densities -- due to the
competition between excluded volume and attractive interactions -- the system
forms a defective network. The very same behavior has been previously observed
in numerical studies of non-spherical models for molecular liquids, such as
water, and in models of patchy colloidal particles. Differently from these
models, theoretical treatments devised for spherical potentials, e.g. integral
equations and ideal mode coupling theory for the glass transition can be
applied in the present case, opening the way for a deeper understanding of the
thermodynamic and dynamic behavior of low valence molecules and particles.Comment: 11 pages, 11 figure
A metabolomic approach to animal vitreous humor topographical composition: A pilot study
The purpose of this study was to evaluate the feasibility of a 1H-NMR-based metabolomic approach to explore the
metabolomic signature of different topographical areas of vitreous humor (VH) in an animal model. Five ocular globes were
enucleated from five goats and immediately frozen at 280uC. Once frozen, three of them were sectioned, and four samples
corresponding to four different VH areas were collected: the cortical, core, and basal, which was further divided into a
superior and an inferior fraction. An additional two samples were collected that were representative of the whole vitreous
body. 1H-NMR spectra were acquired for twenty-three goat vitreous samples with the aim of characterizing the
metabolomic signature of this biofluid and identifying whether any site-specific patterns were present. Multivariate
statistical analysis (MVA) of the spectral data were carried out, including Principal Component Analysis (PCA), Hierarchical
Cluster Analysis (HCA), and Partial Least Squares Discriminant Analysis (PLS-DA). A unique metabolomic signature belonging
to each area was observed. The cortical area was characterized by lactate, glutamine, choline, and its derivatives, N-acetyl
groups, creatine, and glycerol; the core area was characterized by glucose, acetate, and scyllo-inositol; and the basal area
was characterized by branched-chain amino acids (BCAA), betaine, alanine, ascorbate, lysine, and myo-inositol. We propose
a speculative approach on the topographic role of these molecules that are mainly responsible for metabolic differences
among the as-identified areas. 1H-NMR-based metabolomic analysis has shown to be an important tool for investigating the
VH. In particular, this approach was able to assess in the samples here analyzed the presence of different functional areas on
the basis of a different metabolite distribution.The purpose of this study was to evaluate the feasibility of a 1H-NMR-based metabolomic approach to explore the metabolomic signature of different topographical areas of vitreous humor (VH) in an animal model. Five ocular globes were enucleated from five goats and immediately frozen at -80°C. Once frozen, three of them were sectioned, and four samples corresponding to four different VH areas were collected: the cortical, core, and basal, which was further divided into a superior and an inferior fraction. An additional two samples were collected that were representative of the whole vitreous body. 1H-NMR spectra were acquired for twenty-three goat vitreous samples with the aim of characterizing the metabolomic signature of this biofluid and identifying whether any site-specific patterns were present. Multivariate statistical analysis (MVA) of the spectral data were carried out, including Principal Component Analysis (PCA), Hierarchical Cluster Analysis (HCA), and Partial Least Squares Discriminant Analysis (PLS-DA). A unique metabolomic signature belonging to each area was observed. The cortical area was characterized by lactate, glutamine, choline, and its derivatives, N-acetyl groups, creatine, and glycerol; the core area was characterized by glucose, acetate, and scyllo-inositol; and the basal area was characterized by branched-chain amino acids (BCAA), betaine, alanine, ascorbate, lysine, and myo-inositol. We propose a speculative approach on the topographic role of these molecules that are mainly responsible for metabolic differences among the as-identified areas. 1H-NMR-based metabolomic analysis has shown to be an important tool for investigating the VH. In particular, this approach was able to assess in the samples here analyzed the presence of different functional areas on the basis of a different metabolite distribution. © 2014 Locci et al
Self-Assembly of Patchy Particles into Polymer Chains: A Parameter-Free Comparison between Wertheim Theory and Monte Carlo Simulation
We numerically study a simple fluid composed of particles having a hard-core
repulsion, complemented by two short-ranged attractive (sticky) spots at the
particle poles, which provides a simple model for equilibrium polymerization of
linear chains. The simplicity of the model allows for a close comparison, with
no fitting parameters, between simulations and theoretical predictions based on
the Wertheim perturbation theory, a unique framework for the analytic
prediction of the properties of self-assembling particle systems in terms of
molecular parameter and liquid state correlation functions. This theory has not
been subjected to stringent tests against simulation data for ordering across
the polymerization transition. We numerically determine many of the
thermodynamic properties governing this basic form of self-assembly (energy per
particle, order parameter or average fraction of particles in the associated
state, average chain length, chain length distribution, average end-to-end
distance of the chains, and the static structure factor) and find that
predictions of the Wertheim theory accord remarkably well with the simulation
results
Myelodysplastic syndromes: the pediatric point of view.
Myelodysplastic syndromes (MDS) are clonal disorders of the multipotent hematopoietic stem cell characterized by ineffective hematopoiesis and associated with marrow hypercellularity, increased intramedullary cell death and peripheral cytopenias of varying severity. Patients with myelodysplasia have a propensity (20% to 30% of cases) to undergo transformation into acute myeloid leakemia (AML), and a large body of evidence indicates that MDS represent steps in the multiphasic evolution of AML. Progression of the disease is characterized by expansion of the abnormal clone and inhibition of normal hematopoiesis leading to deterioration of the blood cell count and/or development of AML. MDS are relatively unusual in childhood, representing only 3% of pediatric hematological malignancies, although it has been reported that up to 17% of pediatric AML cases may have a previous myelodysplastic phase. The first systematic attempt at morphological classification of MDS was provided by the French-American-British (FAB) group. However, the FAB classification of MDS is only partially applicable in children. Some variants are extremely rare or absent (refractory anemia with ring sideroblasts and chronic myelomonocytic leukemia), and other peculiar pediatric disorders, represented by juvenile chronic myelogenous leukemia (JCML) and the monosomy 7 syndrome, are not included. Moreover, since there is a partial overlap between pediatric MDS and myeloproliferative disorders and the variants occurring in young children have rather specific features, some confusion still surrounds the nosographical definition of childhood MDS, so that none of the proposed classifications are widely accepted and used. Characteristically, some genetic conditions such as Fanconi's anemia, Shwachman's and Down's syndromes predispose to the development of MDS in childhood. The most common variants of childhood MDS are represented by JCML and the monosomy 7 syndrome, both disorders typically occurring in young children. JCML is characterized by a spontaneous growth of granulocyte-macrophage progenitors that show a striking hypersensitivity to granulocyte-macrophage colony-stimulating factor. Clinical presentation resembles that of some myeloproliferative disorders, with massive organomegaly usually not observed in the classically reported variants of MDS. Clinical features of the monosomy 7 syndrome resemble those observed in JCML and a differential diagnosis between these two entities relies upon the higher percentage of fetal hemoglobin, the more pronounced decrease in platelet count and, in some cases, the lack of the peculiar cytogenetic abnormality in the latter. With the number of children being cured of cancer constantly rising, a significant increase in secondary or chemotherapy-related myelodysplasia is being observed, and these disorders represent a formidable challenge for pediatric hematologists due to their poor response to chemotherapy. As a matter of fact, owing to their biological heterogeneity and aggressive clinical course in childhood, all MDS variants pose serious difficulties for successful management. If a compatible donor is available, allogeneic bone marrow transplantation (BMT) becomes the treatment of choice and should be performed during the early stages of the disease. Supportive therapy, differentiative treatments and low-dose chemotherapy, while valuable alternative therapeutic options in adults, have limited application in pediatric patients. The role of intensive chemotherapy and autologous BMT has not yet been clearly defined, and the use of hematopoietic growth factors does not seem to have a significant influence on the natural history of the disease. In the future, new insights into the events leading to progressive genetic changes in the clonal population and into the molecular basis of these genetic lesions could result in interesting new therapeutic approaches directed either at the oncogenes involved in the pathogenesis of the disease, or at the cytokines and/or their receptors causing the abnormal differentiation and proliferation of the myelodysplastic clone
Crystallization Mechanism of Hard Sphere Glasses
In supercooled liquids, vitrification generally suppresses crystallization.
Yet some glasses can still crystallize despite the arrest of diffusive motion.
This ill-understood process may limit the stability of glasses, but its
microscopic mechanism is not yet known. Here we present extensive computer
simulations addressing the crystallization of monodisperse hard-sphere glasses
at constant volume (as in a colloid experiment). Multiple crystalline patches
appear without particles having to diffuse more than one diameter. As these
patches grow, the mobility in neighbouring areas is enhanced, creating dynamic
heterogeneity with positive feedback. The future crystallization pattern cannot
be predicted from the coordinates alone: crystallization proceeds by a sequence
of stochastic micro-nucleation events, correlated in space by emergent dynamic
heterogeneity.Comment: 4 pages 4 figures Accepted for publication in Phys. Rev. Lett., April
201
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