1,988 research outputs found
Toward reliable morphology assessment of thermosets via physical etching: Vinyl ester resin as an example
The morphology of peroxide-cured, styrene crosslinked, bisphenol A-based vinyl ester (VE) resin was investigated by atomic force microscopy (AFM) after ‘physical’ etching with different methods. Etching was achieved by laser ablation, atmospheric plasma treatment and argon ion bombardment. Parameters of the etching were varied to get AFM scans of high topography resolution. VE exhibited a nanoscaled nodular structure the formation of which was ascribed to complex intra- and intermolecular reactions during crosslinking. The microstructure resolved after all the above physical etching techniques was similar provided that optimized etching and suitable AFM scanning conditions were selected. Nevertheless, with respect to the ‘morphology visualization’ these methods follow the power ranking: argon bombardment > plasma treatment > laser ablation
Components of the ubiquitin-proteasome pathway compete for surfaces on Rad23 family proteins
Background: The delivery of ubiquitinated proteins to the proteasome for degradation is a key step in the regulation of the ubiquitin-proteasome pathway, yet the mechanisms underlying this step are not understood in detail. The Rad23 family of proteins is known to bind ubiquitinated proteins through its two ubiquitin-associated (UBA) domains, and may participate in the delivery of ubiquitinated proteins to the proteasome through docking via the Rad23 ubiquitin-like (UBL) domain.
Results: In this study, we investigate how the interaction between the UBL and UBA domains may modulate ubiquitin recognition and the delivery of ubiquitinated proteins to the proteasome by autoinhibition. We have explored a competitive binding model using specific mutations in the UBL domain. Disrupting the intramolecular UBL-UBA domain interactions in HHR23A indeed potentiates ubiquitin-binding. Additionally, the analogous surface on the Rad23 UBL domain overlaps with that required for interaction with both proteasomes and the ubiquitin ligase Ufd2. We have found that mutation of residues on this surface affects the ability of Rad23 to deliver ubiquitinated proteins to the proteasome.
Conclusions: We conclude that the competition of ubiquitin-proteasome pathway components for surfaces on Rad23 is important for the role of the Rad23 family proteins in proteasomal targeting
Simulating ice core 10Be on the glacial–interglacial timescale
10Be ice core measurements are an important tool for paleoclimate research, e.g., allowing for the reconstruction of past solar activity or changes in the geomagnetic dipole field. However, especially on multi-millennial timescales, the share of production and climate-induced variations of respective 10Be ice core records is still up for debate. Here we present the first quantitative climatological model of the 10Be ice concentration up to the glacial–interglacial timescale. The model approach is composed of (i) a coarse resolution global atmospheric transport model and (ii) a local 10Be air–firn transfer model. Extensive global-scale observational data of short-lived radionuclides as well as new polar 10Be snow-pit measurements are used for model calibration and validation. Being specifically configured for 10Be in polar ice, this tool thus allows for a straightforward investigation of production- and non-production-related modulation of this nuclide. We find that the polar 10Be ice concentration does not immediately record the globally mixed cosmogenic production signal. Using geomagnetic modulation and revised Greenland snow accumulation rate changes as model input, we simulate the observed Greenland Summit (GRIP and GISP2) 10Be ice core records over the last 75 kyr (on the GICC05modelext timescale). We show that our basic model is capable of reproducing the largest portion of the observed 10Be changes. However, model–measurement differences exhibit multi-millennial trends (differences up to 87% in case of normalized to the Holocene records) which call for closer investigation. Focusing on the (12–37) b2k (before the year AD 2000) period, mean model–measurement differences of 30% cannot be attributed to production changes. However, unconsidered climate-induced changes could likely explain the model–measurement mismatch. In fact, the 10Be ice concentration is very sensitive to snow accumulation changes. Here the reconstructed Greenland Summit (GRIP) snow accumulation rate record would require revision of +28% to solely account for the (12–37) b2k model–measurement differences
Self-Pulsating Semiconductor Lasers: Theory and Experiment
We report detailed measurements of the pump-current dependency of the
self-pulsating frequency of semiconductor CD lasers. A distinct kink in this
dependence is found and explained using rate-equation model. The kink denotes a
transition between a region where the self-pulsations are weakly sustained
relaxation oscillations and a region where Q-switching takes place. Simulations
show that spontaneous emission noise plays a crucial role for the cross-over.Comment: Revtex, 16 pages, 7 figure
Overcoming of Information Extremism as a Condition of Human and Social Wellbeing
The urgency of this research is indisputable because the subjects of information extremism having at their disposal various mass media can disseminate their beliefs and ideas over thousands of people in different countries all over the world. International collaboration is needed to struggle against this evil. The paper provides deep insight into the notion "information extremism", as well as its classification and forms. It is aimed at revealing the specific character of the notions "information extremism" and "information terrorism" and their investigation. Illocutionary influence as the major method of information extremism is highlighted in the paper. The authors demonstrate important influence of mass media including the Internet over readership, especially the youth. It is concluded that the issue of information extremism requires for more rigorous and deeper consideration. It is necessary to fight against forms of its manifestation, because they have a significant effect on homeland and international safety and stability
A Conditional Yeast E1 Mutant Blocks the Ubiquitin–Proteasome Pathway and Reveals a Role for Ubiquitin Conjugates in Targeting Rad23 to the Proteasome
E1 ubiquitin activating enzyme catalyzes the initial step in all ubiquitin-dependent processes. We report the isolation of uba1-204, a temperature-sensitive allele of the essential Saccharomyces cerevisiae E1 gene, UBA1. Uba1-204 cells exhibit dramatic inhibition of the ubiquitin–proteasome system, resulting in rapid depletion of cellular ubiquitin conjugates and stabilization of multiple substrates. We have employed the tight phenotype of this mutant to investigate the role ubiquitin conjugates play in the dynamic interaction of the UbL/UBA adaptor proteins Rad23 and Dsk2 with the proteasome. Although proteasomes purified from mutant cells are intact and proteolytically active, they are depleted of ubiquitin conjugates, Rad23, and Dsk2. Binding of Rad23 to these proteasomes in vitro is enhanced by addition of either free or substrate-linked ubiquitin chains. Moreover, association of Rad23 with proteasomes in mutant and wild-type cells is improved upon stabilizing ubiquitin conjugates with proteasome inhibitor. We propose that recognition of polyubiquitin chains by Rad23 promotes its shuttling to the proteasome in vivo
Organic molecular markers and signature from wood combustion particles in winter ambient aerosols: aerosol mass spectrometer (AMS) and high time-resolved GC-MS measurements in Augsburg, Germany
The impact of wood combustion on ambient aerosols was investigated in
Augsburg, Germany during a winter measurement campaign of a six-week period.
Special attention was paid to the high time resolution observations of wood
combustion with different mass spectrometric methods. Here we present and
compare the results from an Aerodyne aerosol mass spectrometer (AMS) and gas
chromatographic – mass spectrometric (GC-MS) analysed PM<sub>1</sub> filters on an
hourly basis. This includes source apportionment of the AMS derived organic
matter (OM) using positive matrix factorisation (PMF) and analysis of
levoglucosan as wood combustion marker, respectively.
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During the measurement period nitrate and OM mass are the main contributors
to the defined submicron particle mass of AMS and Aethalometer with 28%
and 35%, respectively. Wood combustion organic aerosol (WCOA) contributes
to OM with 23% on average and 27% in the evening and night time.
Conclusively, wood combustion has a strong influence on the organic matter
and overall aerosol composition. Levoglucosan accounts for 14% of WCOA
mass with a higher percentage in comparison to other studies. The ratio
between the mass of levoglucosan and organic carbon amounts to 0.06.
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This study is unique in that it provides a one-hour time resolution
comparison between the wood combustion results of the AMS and the GC-MS
analysed filter method at a PM<sub>1</sub> particle size range. The comparison of
the concentration variation with time of the PMF WCOA factor, levoglucosan
estimated by the AMS data and the levoglucosan measured by GC-MS is highly
correlated (<i>R</i><sup>2</sup> = 0.84), and a detailed discussion on the contributors
to the wood combustion marker ion at mass-to-charge ratio 60 is given. At
the end, both estimations, the WCOA factor and the levoglucosan
concentration estimated by AMS data, allow to observe the variation with
time of wood combustion emissions (gradient correlation with GC-MS
levoglucosan of <i>R</i><sup>2</sup> = 0.84). In the case of WCOA, it provides the
estimated magnitude of wood combustion emission. Quantitative estimation of
the levoglucosan concentration from the AMS data is problematic due to its
overestimation in comparison to the levoglucosan measured by the GC-MS
Internally Electrodynamic Particle Model: Its Experimental Basis and Its Predictions
The internally electrodynamic (IED) particle model was derived based on
overall experimental observations, with the IED process itself being built
directly on three experimental facts, a) electric charges present with all
material particles, b) an accelerated charge generates electromagnetic waves
according to Maxwell's equations and Planck energy equation and c) source
motion produces Doppler effect. A set of well-known basic particle equations
and properties become predictable based on first principles solutions for the
IED process; several key solutions achieved are outlined, including the de
Broglie phase wave, de Broglie relations, Schr\"odinger equation, mass,
Einstein mass-energy relation, Newton's law of gravity, single particle self
interference, and electromagnetic radiation and absorption; these equations and
properties have long been broadly experimentally validated or demonstrated. A
specific solution also predicts the Doebner-Goldin equation which emerges to
represent a form of long-sought quantum wave equation including gravity. A
critical review of the key experiments is given which suggests that the IED
process underlies the basic particle equations and properties not just
sufficiently but also necessarily.Comment: Presentation at the 27th Int Colloq on Group Theo Meth in Phys, 200
A finite model of two-dimensional ideal hydrodynamics
A finite-dimensional su() Lie algebra equation is discussed that in the
infinite limit (giving the area preserving diffeomorphism group) tends to
the two-dimensional, inviscid vorticity equation on the torus. The equation is
numerically integrated, for various values of , and the time evolution of an
(interpolated) stream function is compared with that obtained from a simple
mode truncation of the continuum equation. The time averaged vorticity moments
and correlation functions are compared with canonical ensemble averages.Comment: (25 p., 7 figures, not included. MUTP/92/1
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