The rise and fall of the ancient northern pike master sex-determining gene

The understanding of the evolution of variable sex determination mechanisms across taxa requires comparative studies among closely related species. Following the fate of a known master sex-determining gene, we traced the evolution of sex determination in an entire teleost order (Esociformes). We discovered that the northern pike (Esox lucius) master sex-determining gene originated from a 65 to 90 million-year-old gene duplication event and that it remained sex linked on undifferentiated sex chromosomes for at least 56 million years in multiple species. We identified several independent species- or population-specific sex determination transitions, including a recent loss of a Y chromosome. These findings highlight the diversity of evolutionary fates of master sex-determining genes and the importance of population demographic history in sex determination studies. We hypothesize that occasional sex reversals and genetic bottlenecks provide a non-adaptive explanation for sex determination transitions

Integrative genomic analysis reveals somatic mutations in pheochromocytoma and

Pheochromocytomas and paragangliomas are neuroendocrine tumors that occur in the context of inherited cancer syndromes in ∼30% of cases and are linked to germline mutations in the VHL, RET, NF1, SDHA, SDHB, SDHC, SDHD, SDHAF2 and TMEM127 genes. Although genome-wide expression studies have revealed some of the mechanisms likely to be involved in pheochromocytoma/paraganglioma tumorigenesis, the complete molecular distinction of all subtypes of hereditary tumors has not been solved and the genetic events involved in the generation of sporadic tumors are unknown. With these purposes in mind, we investigated 202 pheochromocytomas/paragangliomas, including 75 hereditary tumors, using expression profiling, BAC array comparative genomic hybridization and somatic mutation screening. Gene expression signatures defined the hereditary tumors according to their genotype and notably, led to a complete subseparation between SDHx-and VHL-related tumors. In tumor tissues, the systematic characterization of somatic genetic events associated with germline mutations in tumor suppressor genes revealed loss of heterozygosity (LOH) in a majority of cases, but also detected point mutations and copy-neutral LOH. Finally, guided by transcriptome classifications and LOH profiles, somatic mutations in VHL or RET genes were identified in 14% of sporadic pheochromocytomas/paragangliomas. Overall, we found a germline or somatic genetic alteration in 45.5% (92/202) of the tumors in this large series of pheochromocytomas/paragangliomas. Regarding mutated genes, specific molecular pathways involved in tumorigenesis mechanisms are identified. Altogether, these new findings suggest that somatic mutation analysis is likely to yield important clues for personalizing molecular targeted therapies

The rise and fall of the ancient northern pike master sex determining gene

The understanding of the evolution of variable sex determination mechanisms across taxa requires comparative studies among closely related species. Following the fate of a known master sex-determining gene, we traced the evolution of sex determination in an entire teleost order (Esociformes). We discovered that the northern pike (Esox lucius) master sex-determining gene originated from a 65 to 90 million-year-old gene duplication event and that it remained sex-linked on undifferentiated sex chromosomes for at least 56 million years in multiple species. We identified several independent species- or population-specific sex determination transitions, including a recent loss of a Y-chromosome. These findings highlight the diversity of evolutionary fates of master sex-determining genes and the importance of population demographic history in sex determination studies. We hypothesize that occasional sex reversals and genetic bottlenecks provide a non-adaptive explanation for sex determination transitions

Caractérisation du phénotype gonadique de truites génétiquement femelles porteuses d'une mutation masculinisante mutation <em>"mal"</em>

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Follistatin Is an Early Player in Rainbow Trout Ovarian Differentiation and Is Both Colocalized with Aromatase and Regulated by the Wnt Pathway.

International audienceIn mammals, follistatin (FST) plays an important role in early ovarian differentiation, acting downstream of the Wnt pathway. In teleost fish, fst is implicated in folliculogenesis and oocyte maturation, and an early and specific expression during ovarian differentiation has been described in rainbow trout, Oncorhynchus mykiss. By in situ hybridization, we demonstrated that during rainbow trout gonadal differentiation, fst shares a similar expression pattern with cyp19a1a, the gene encoding ovarian aromatase, a key steroidogenic enzyme needed for ovarian differentiation in fish. Expression of fst and cyp19a1a was first detected in a few scattered cells in the embryonic ovary several days before hatching. Then, after histological differentiation, fst and cyp19a1a expression was localized in clusters of cells lining the future ovarian lamellae. As FST expression is known to be induced by the Wnt/β-catenin pathway in mammals, the Wnt pathway was inhibited in vivo with the IWR-1 molecule, and we analyzed by qPCR the effects of this treatment on fst expression. We found that IWR-1 decreased fst expression in female gonads, consistent with a regulation of fst expression by the Wnt pathway in rainbow trout. Furthermore, expression of cyp19a1a was also downregulated, suggesting an implication of the Wnt pathway in ovarian differentiation

The sexually dimorphic on the Y-chromosome gene (sdY) is a conserved male-specific Y-chromosome sequence in many salmonids

All salmonid species investigated to date have been characterized with a male heterogametic sex-determination system. However, as these species do not share any Y-chromosome conserved synteny, there remains a debate on whether they share a common master sex-determining gene. In this study, we investigated the extent of conservation and evolution of the rainbow trout (Oncorhynchus mykiss) master sex-determining gene, sdY (sexually dimorphic on the Y-chromosome), in 15 different species of salmonids. We found that the sdY sequence is highly conserved in all salmonids and that sdY is a male-specific Y-chromosome gene in the majority of these species. These findings demonstrate that most salmonids share a conserved sex-determining locus and also strongly suggest that sdY may be this conserved master sex-determining gene. However, in two whitefish species (subfamily Coregoninae), sdY was found both in males and females, suggesting that alternative sex-determination systems may have also evolved in this family. Based on the wide conservation of sdY as a male-specific Y-chromosome gene, efficient and easy molecular sexing techniques can now be developed that will be of great interest for studying these economically and environmentally important species

Sea surface salinity signature of the tropical Atlantic interannual climatic modes

Plain Language Summary The characteristic sea surface salinity (SSS) patterns associated with the tropical Atlantic meridional and equatorial interannual modes are extracted from in situ observations, by a statistical analysis performed on the 1980-2012 period. These SSS signatures of the interannual climatic modes are reproduced in a regional numerical simulation. For each mode, oceanic and/or atmospheric processes driving the SSS signature are identified through a mixed-layer salt budget in the validated model. During a positive meridional mode in spring, a northward shift of the Intertropical Convergence Zone and related precipitation maximum creates a south-north dipole of positive-negative SSS anomalies around the equator. Western boundary currents strengthen and advect relatively fresh equatorial waters, which creates negative SSS anomalies in the north and south west tropical Atlantic. Meridional and vertical advection create positive SSS anomalies off the Congo River. During a positive equatorial mode in summer, a southward shift of the Intertropical Convergence Zone-related rainfall maximum creates a south-north dipole of negative-positive SSS anomalies between the equator and 10 degrees N. Meridional advection also contributes to the positive SSS anomalies between 5 degrees N and 10 degrees N. Vertical advection and diffusion at the mixed-layer base create positive SSS anomalies between 5 degrees S and the equator. Horizontal advection creates large SSS anomalies in the North Brazil Current retroflection region, negative along the coast and positive further offshore. The SSS signatures of the meridional and equatorial modes described above are well captured by the Soil Moisture-Ocean Salinity satellite during the 2010 and 2012 events. This study shows that both meridional and equatorial interannual climatic modes impact the sea surface salinity (SSS) in tropical Atlantic through atmospheric and/or oceanic processes. The atmospheric forcing, related to Intertropical Convergence Zone migration, controls the equatorial region, while the advection, due to modulation of current dynamics, vertical SSS gradient, and mixing at the base of mixed layer, drives SSS in the region under the influence of river plumes

Response of the Bight of Benin (Gulf of Guinea, West Africa) coastline to anthropogenic and natural forcing : Part 1 : Wave climate variability and impacts on the longshore sediment transport

The short, medium and long-term evolution of the sandy coastline of the Bight of Benin in the Gulf of Guinea, West Africa, has become a major regional focal point due to the rapid socio-economic development that is occurring in the region, including rapid urbanization and a sharp increase in harbor-based trade. Harbors have a significant impact on the present evolution of this coast, notably by affecting longshore sediment transport. However, little is known of the environmental drivers, notably the wave climate, that governs longshore sediment transport and the ensuing pattern of shoreline evolution of this coastal zone. This article aims to address this important knowledge gap by providing a general overview of coastal evolution in the Bight of Benin and the physical processes that control this evolution. Here, the 1979-2012 ERA-Interim hindcast is used to understand the temporal dynamics of longshore sediment transport. Oblique waves (annual average H-s,=1.36 m, T-p=9.6 s, S-SW incidence) drive an eastward drift of approximately 500,000 m(3)/yr. The waves driving this large longshore transport can be separated into two components with distinct origins and behavior: wind waves generated locally in the Gulf of Guinea and swell waves generated in the southern hemisphere sub- (30-35 degrees S), and extra-tropics (45-60 degrees S). The analysis undertaken here shows that the contribution to the gross annual longshore transport from swell wave-driven longshore currents is an order of magnitude larger than the local wind wave-driven longshore currents. Swell waves are dominantly generated by westerlies in the 40-60 degrees S zone and to a lesser extent by trade winds at 30-35 degrees S. The longshore sediment drift decay (-5% over 1979-2012) is found to be linked with a decrease in the intensity of westerly winds associated with their southward shift, in addition to a strengthening of the trade winds, which reduces the eastward sediment transport potential. The equatorial fluctuation of the Inter-Tropical Convergence Zone (ITCZ) is found to explain most of the variability in transport induced by wind waves, while the Southern Annular Mode (SAM), an extra-tropical mode, has a predominant influence on transport induced by swell waves. The ITCZ and SAM have, respectively, a negative and positive trend over the period 1979-2012 that explains the decrease in both wind- and swell-wave-induced transport. For future scenarii, General Circulation Models (GCMs) predict a stabilization of the SAM, and, thus, a non-substantial or weak change in longshore sediment transport can be expected on the coast of the Bight of Benin

Characterization of a conserved master sex-determining gene in salmonids

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SEXYTROUT - Caractérisation du phénotype gonadique d'une population de truite arc-en-ciel porteuse d'une mutation masculinisante (mutation "mal" (2009-2012)

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