138 research outputs found
Prenatal stress exposure and multimodal assessment of amygdala-medial prefrontal cortex connectivity in infants
Stressful experiences are linked to neurodevelopment. There is growing interest in the role of stress in the connectivity between the amygdala and medial prefrontal cortex (mPFC), a circuit that subserves automatic emotion regulation. However, the specific timing and mechanisms that underlie the association between stress and amygdala-mPFC connectivity are unclear. Many factors, including variations in fetal exposure to maternal stress, appear to affect early developing brain circuitry. However, few studies have examined the associations of stress and amygdala-mPFC connectivity in early life, when the brain is most plastic and sensitive to environmental influence. In this longitudinal pilot study, we characterized the association between prenatal stress and amygdala-mPFC connectivity in young infants (approximately age 5 weeks). A final sample of 33 women who provided data on preconception and prenatal stress during their pregnancy returned with their offspring for a magnetic resonance imaging scan session, which enabled us to characterize amygdala-mPFC structural and functional connectivity as a function of prenatal stress. Increased prenatal stress was associated with decreased functional connectivity and increased structural connectivity between the amygdala and mPFC. These results provide insight into the influence of prenatal maternal stress on the early development of this critical regulatory circuitry
Radio and IR interferometry of SiO maser stars
Radio and infrared interferometry of SiO maser stars provide complementary
information on the atmosphere and circumstellar environment at comparable
spatial resolution. Here, we present the latest results on the atmospheric
structure and the dust condensation region of AGB stars based on our recent
infrared spectro-interferometric observations, which represent the environment
of SiO masers. We discuss, as an example, new results from simultaneous VLTI
and VLBA observations of the Mira variable AGB star R Cnc, including VLTI near-
and mid-infrared interferometry, as well as VLBA observations of the SiO maser
emission toward this source. We present preliminary results from a monitoring
campaign of high-frequency SiO maser emission toward evolved stars obtained
with the APEX telescope, which also serves as a precursor of ALMA images of the
SiO emitting region. We speculate that large-scale long-period chaotic motion
in the extended molecular atmosphere may be the physical reason for observed
deviations from point symmetry of atmospheric molecular layers, and for the
observed erratic variability of high-frequency SiO maser emissionComment: 8 pages, 4 figures, submitted to Proc. IAU Symp. 287 "Cosmic masers -
from OH to H_0", R.S. Booth, E.M.L. Humphreys, W.H.T. Vlemmings (eds.),
invited pape
PTEN protein loss by immunostaining: Analytic validation and prognostic indicator for a high risk surgical cohort of prostate cancer patients
PURPOSE: Analytically validated assays to interrogate biomarker status in clinical samples are crucial for personalized medicine. PTEN is a tumor suppressor commonly inactivated in prostate cancer that has been mechanistically linked to disease aggressiveness. Though deletion of PTEN, as detected by cumbersome fluorescence in situ hybridization (FISH) spot counting assays, is associated with poor prognosis, few studies have validated immunohistochemical (IHC) assays to determine whether loss of PTEN protein is associated with unfavorable disease. EXPERIMENTAL DESIGN: PTEN IHC was validated by employing formalin fixed and paraffin embedded isogenic human cell lines containing or lacking intact PTEN alleles. PTEN IHC was 100% sensitive and 97.8% specific for detecting genomic alterations in 58 additional cell lines. PTEN protein loss was then assessed on 376 prostate tumor samples, and PTEN FISH or high resolution SNP microarray analysis was performed on a subset of these cases. RESULTS: PTEN protein loss, as assessed as a dichotomous IHC variable, was highly reproducible, correlated strongly with adverse pathologic features (e.g. Gleason score and pathological stage), detected between 75% and 86% of cases with PTEN genomic loss, and was found at times in the absence of apparent genomic loss. In a cohort of 217 high risk surgically treated patients, PTEN protein loss was associated with decreased time to metastasis. CONCLUSIONS: These studies validate a simple method to interrogate PTEN status in clinical specimens and support the utility of this test in future multi-center studies, clinical trials and ultimately perhaps for routine clinical care
Prevalence of the HOXB13 G84E prostate cancer risk allele in men treated with radical prostatectomy
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106912/1/bju12522.pd
Drawing firmer conclusions: autistic children show no evidence of a local processing bias in a controlled copying task
Drawing tasks are frequently used to test competing theories of visuospatial skills in autism. Yet, methodological differences between studies have led to inconsistent findings. To distinguish between accounts based on local bias or global deficit, we present a simple task that has previously revealed dissociable local/global impairments in neuropsychological patients. Autistic and typical children copied corner elements, arranged in a square configuration. Grouping cues were manipulated to test whether global properties affected the accuracy of reproduction. All children were similarly affected by these manipulations. There was no group difference in the reproduction of local elements, although global accuracy was negatively related to better local processing for autistic children. These data speak against influential theories of visuospatial differences in autism
The ALMA Interferometric Pipeline Heuristics
We describe the calibration and imaging heuristics developed and deployed in
the ALMA interferometric data processing pipeline, as of ALMA Cycle 9. The
pipeline software framework is written in Python, with each data reduction
stage layered on top of tasks and toolkit functions provided by the Common
Astronomy Software Applications package. This framework supports a variety of
tasks for observatory operations, including science data quality assurance,
observing mode commissioning, and user reprocessing. It supports ALMA and VLA
interferometric data along with ALMA and NRO45m single dish data, via different
stages and heuristics. In addition to producing calibration tables, calibrated
measurement sets, and cleaned images, the pipeline creates a WebLog which
serves as the primary interface for verifying the data quality assurance by the
observatory and for examining the contents of the data by the user. Following
the adoption of the pipeline by ALMA Operations in 2014, the heuristics have
been refined through annual development cycles, culminating in a new pipeline
release aligned with the start of each ALMA Cycle of observations. Initial
development focused on basic calibration and flagging heuristics (Cycles 2-3),
followed by imaging heuristics (Cycles 4-5), refinement of the flagging and
imaging heuristics with parallel processing (Cycles 6-7), addition of the
moment difference analysis to improve continuum channel identification (2020
release), addition of a spectral renormalization stage (Cycle 8), and
improvement in low SNR calibration heuristics (Cycle 9). In the two most recent
Cycles, 97% of ALMA datasets were calibrated and imaged with the pipeline,
ensuring long-term automated reproducibility. We conclude with a brief
description of plans for future additions, including self-calibration,
multi-configuration imaging, and calibration and imaging of full polarization
data.Comment: accepted for publication by Publications of the Astronomical Society
of the Pacific, 65 pages, 20 figures, 10 tables, 2 appendice
Behavioral Coping Phenotypes and Associated Psychosocial Outcomes of Pregnant and Postpartum Women During the COVID-19 Pandemic
The impact of COVID-19-related stress on perinatal women is of heightened public health concern given the established intergenerational impact of maternal stress-exposure on infants and fetuses. There is urgent need to characterize the coping styles associated with adverse psychosocial outcomes in perinatal women during the COVID-19 pandemic to help mitigate the potential for lasting sequelae on both mothers and infants. This study uses a data-driven approach to identify the patterns of behavioral coping strategies that associate with maternal psychosocial distress during the COVID-19 pandemic in a large multicenter sample of pregnant women (N = 2876) and postpartum women (N = 1536). Data was collected from 9 states across the United States from March to October 2020. Women reported behaviors they were engaging in to manage pandemic-related stress, symptoms of depression, anxiety and global psychological distress, as well as changes in energy levels, sleep quality and stress levels. Using latent profile analysis, we identified four behavioral phenotypes of coping strategies. Critically, phenotypes with high levels of passive coping strategies (increased screen time, social media, and intake of comfort foods) were associated with elevated symptoms of depression, anxiety, and global psychological distress, as well as worsening stress and energy levels, relative to other coping phenotypes. In contrast, phenotypes with high levels of active coping strategies (social support, and self-care) were associated with greater resiliency relative to other phenotypes. The identification of these widespread coping phenotypes reveals novel behavioral patterns associated with risk and resiliency to pandemic-related stress in perinatal women. These findings may contribute to early identification of women at risk for poor long-term outcomes and indicate malleable targets for interventions aimed at mitigating lasting sequelae on women and children during the COVID-19 pandemic
Amyloid-Associated Nucleic Acid Hybridisation
Nucleic acids promote amyloid formation in diseases including Alzheimer's
and Creutzfeldt-Jakob disease. However, it remains unclear whether the close
interactions between amyloid and nucleic acid allow nucleic acid secondary
structure to play a role in modulating amyloid structure and function. Here we
have used a simplified system of short basic peptides with alternating
hydrophobic and hydrophilic amino acid residues to study nucleic acid - amyloid
interactions. Employing biophysical techniques including X-ray fibre
diffraction, circular dichroism spectroscopy and electron microscopy we show
that the polymerized charges of nucleic acids concentrate and enhance the
formation of amyloid from short basic peptides, many of which would not
otherwise form fibres. In turn, the amyloid component binds nucleic acids and
promotes their hybridisation at concentrations below their solution
Kd, as shown by time-resolved FRET studies. The
self-reinforcing interactions between peptides and nucleic acids lead to the
formation of amyloid nucleic acid (ANA) fibres whose properties are distinct
from their component polymers. In addition to their importance in disease and
potential in engineering, ANA fibres formed from prebiotically-produced peptides
and nucleic acids may have played a role in early evolution, constituting the
first entities subject to Darwinian evolution
TeraHertz Exploration and Zooming-in for Astrophysics (THEZA): ESA Voyage 2050 White Paper
This paper presents the ESA Voyage 2050 White Paper for a concept of
TeraHertz Exploration and Zooming-in for Astrophysics (THEZA). It addresses the
science case and some implementation issues of a space-borne radio
interferometric system for ultra-sharp imaging of celestial radio sources at
the level of angular resolution down to (sub-) microarcseconds. THEZA focuses
at millimetre and sub-millimetre wavelengths (frequencies above 300~GHz),
but allows for science operations at longer wavelengths too. The THEZA concept
science rationale is focused on the physics of spacetime in the vicinity of
supermassive black holes as the leading science driver. The main aim of the
concept is to facilitate a major leap by providing researchers with orders of
magnitude improvements in the resolution and dynamic range in direct imaging
studies of the most exotic objects in the Universe, black holes. The concept
will open up a sizeable range of hitherto unreachable parameters of
observational astrophysics. It unifies two major lines of development of
space-borne radio astronomy of the past decades: Space VLBI (Very Long Baseline
Interferometry) and mm- and sub-mm astrophysical studies with "single dish"
instruments. It also builds upon the recent success of the Earth-based Event
Horizon Telescope (EHT) -- the first-ever direct image of a shadow of the
super-massive black hole in the centre of the galaxy M87. As an amalgam of
these three major areas of modern observational astrophysics, THEZA aims at
facilitating a breakthrough in high-resolution high image quality studies in
the millimetre and sub-millimetre domain of the electromagnetic spectrum.Comment: White Paper submitted in response to the ESA Call Voyage 205
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