Društveno-edukativni centar za starije osobe : diplomski rad

Generalni trend starenja stanovništva postavlja pitanje ponude sadržaja specijaliziranih za starije osobe. U razvijenijim državama zapada centri, klubovi, organizacije pa čak i sveučilišta za 3. životnu dob već su ustaljena pojava. Polazeći od činjenice da su umirovljenici kod nas zanemarivana skupina o kojoj se misli prvenstveno u kontekstu zbrinjavanja, a ne aktiviranja, projektom želim ponuditi prostor koji će služiti kao okupljalište starijih osoba u produktivnom provođenju vremenu te djelovati protiv predrasuda o starijim osobama kao pasivnom dijelu društva

Društveno-edukativni centar za starije osobe : diplomski rad

Generalni trend starenja stanovništva postavlja pitanje ponude sadržaja specijaliziranih za starije osobe. U razvijenijim državama zapada centri, klubovi, organizacije pa čak i sveučilišta za 3. životnu dob već su ustaljena pojava. Polazeći od činjenice da su umirovljenici kod nas zanemarivana skupina o kojoj se misli prvenstveno u kontekstu zbrinjavanja, a ne aktiviranja, projektom želim ponuditi prostor koji će služiti kao okupljalište starijih osoba u produktivnom provođenju vremenu te djelovati protiv predrasuda o starijim osobama kao pasivnom dijelu društva

Potentially toxic planktic and benthic Cyanobacteria in Slovenian freshwater bodies: detection by quantitative PCR

Due to increased frequency of cyanobacterial blooms and emerging evidence of cyanotoxicity in biofilm, reliable methods for early cyanotoxin threat detection are of major importance for protection of human, animal and environmental health. To complement the current methods of risk assessment, this study aimed to evaluate selected qPCR assays for detection of potentially toxic cyanobacteria in environmental samples. In the course of one year, 25 plankton and 23 biofilm samples were collected from 15 water bodies in Slovenia. Three different analyses were performed and compared to each other; qPCR targeting mcyE, cyrJ and sxtA genes involved in cyanotoxin production, LC-MS/MS quantifying microcystin, cylindrospermopsin and saxitoxin concentration, and microscopic analyses identifying potentially toxic cyanobacterial taxa. qPCR analyses detected potentially toxic Microcystis in 10 lake plankton samples, and potentially toxic Planktothrix cells in 12 lake plankton and one lake biofilm sample. A positive correlation was observed between numbers of mcyE gene copies and microcystin concentrations. Potential cylindrospermopsin- and saxitoxin-producers were detected in three and seven lake biofilm samples, respectively. The study demonstrated a potential for cyanotoxin production that was left undetected by traditional methods in both plankton and biofilm samples. Thus, the qPCR method could be useful in regular monitoring of water bodies to improve risk assessment and enable timely measures

The advantages of flow cytometry in comparison to fluorometric measurement in algal toxicity test

Fluorometric measurements in algal toxicity tests are very commonly used as surrogate parameters for algal biomass. Although, fluorometry is a powerful technique, we have demonstrated that it is not suitable for determination of toxic effects of chemicals, which alter the fluorescence spectra. We recommend the flow cytometry as the best technique for detecting algal and/or cyanobacterial cell count and fluorescence per cell. Flow cytometry has many advantages: little volume of algal/cyanobacterial sample required, suitable also for little algal/cyanobacterial cells, distinction between live and dead cells. Furthermore, flow cytometry reveals the early changes in fluorescence spectra as a consequence of the specific chemicals presence or stress, even though the cell count is not yet affected (an early marker for ecotoxicology testing)