338 research outputs found
Evaluation of human umbilical cord blood as a source of embryonic stem cells
Human umbilical cord blood (HUCB) has been poorly characterised as a source of embryonic stem cells (ESCs). The aim of this study, therefore, was to evaluate HUCB as source of mesenchymal stem cells (MSCs) with embryonic characteristics. HUCB was collected from consenting women undergoing elective caesarean sections. HUCB was meticulously explanted into MesenCult media and incubated. Qualitative and quantitative immunophenotyping of cells was achieved using fluorescein isothiocyanate (FITC) labelled antibodies (CD34, CD45, CD29, CD44, CD73 and CD105) phenotypic markers. Immunocytochemistry was carried out for the human ESC markers CD9, stage-specific embryonic antigen-1 and 4 (SSEA-1 and SSEA-4), E-cadherin, Podocalyxin (PODXL), sex-determining region Y-box 2 (SOX2), NANOG and Octamer (OCT3/4). MSCs were cultured to induce differentiation into adipogenic, osteogenic, chondrogenic and neurogenic cells. Immunocytochemistry was used to identify fatty acid binding protein-4 (FABP-4), osteocalcin, aggrecan, SOX2 and oligodendrocyte-4 (Olig-4) markers. The cells were strongly positive for the MSC markers CD29, CD44, CD73 and CD105; these cells also expressed the ESC markers CD9, SSEA-1 and SSEA-4, E-cadherin, PODXL, SOX2, NANOG and OCT3/4. Additionally, the MSCs expressed the adipogenic FABP-4, osteogenic osteocalcin, chondrogenic aggrecan and neural Olig-4 and SOX2 markers after differentiation. Therefore, HUCB is a rich source for MSCs with embryonic characteristics
ΠΠ΅ΡΠΎΠ΄ΠΈΡΠ½Ρ Π²ΠΊΠ°Π·ΡΠ²ΠΊΠΈ Π΄ΠΎ Π²ΠΈΠΊΠΎΠ½Π°Π½Π½Ρ Π΄ΠΈΠΏΠ»ΠΎΠΌΠ½ΠΈΡ ΠΏΡΠΎΠ΅ΠΊΡΡΠ² ΡΠ° ΡΠΎΠ±ΡΡ Π΄Π»Ρ Π·Π΄ΠΎΠ±ΡΡΡΡ ΠΎΡΠ²ΡΡΠ½ΡΠΎ-ΠΊΠ²Π°Π»ΡΡΡΠΊΠ°ΡΡΠΉΠ½ΠΎΠ³ΠΎ ΡΡΠ²Π½Ρ Β«Π±Π°ΠΊΠ°Π»Π°Π²ΡΒ» Π·Π° Π½Π°ΠΏΡΡΠΌΠΎΠΌ ΠΏΡΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ 6.050701 ΠΠ»Π΅ΠΊΡΡΠΎΡΠ΅Ρ Π½ΡΠΊΠ° ΡΠ° Π΅Π»Π΅ΠΊΡΡΠΎΡΠ΅Ρ Π½ΠΎΠ»ΠΎΠ³ΡΡ ΡΠ° ΡΡΠ²Π½Ρ Β«ΡΠΏΠ΅ΡΡΠ°Π»ΡΡΡΒ» Π·Π° ΡΠΏΠ΅ΡΡΠ°Π»ΡΠ½ΡΡΡΡ Β«ΠΠ»Π΅ΠΊΡΡΠΎΡΠ΅Ρ Π½ΡΡΠ½Ρ ΡΠΈΡΡΠ΅ΠΌΠΈ Π΅Π»Π΅ΠΊΡΡΠΎΡΠΏΠΎΠΆΠΈΠ²Π°Π½Π½ΡΒ»
ΠΠ΅ΡΠΎΠ΄ΠΈΡΠ½Ρ Π²ΠΊΠ°Π·ΡΠ²ΠΊΠΈ ΠΌΡΡΡΡΡΡ Π²ΠΈΠΌΠΎΠ³ΠΈ Π΄ΠΎ ΠΊΠ²Π°Π»ΡΡΡΠΊΠ°ΡΡΠΉΠ½ΠΈΡ
ΡΠΎΠ±ΡΡ,
ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ°ΡΠΈΠΊΡ, Π·Π° ΡΠΊΠΎΡ Π²ΠΈΠ·Π½Π°ΡΠ°ΡΡΡΡΡ ΡΠ΅ΠΌΠΈ, ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄Π°ΡΡΡ Π· ΠΎΡΠΎΡΠΌΠ»Π΅Π½Π½Ρ ΡΡ
ΡΠΊΠ»Π°Π΄ΠΎΠ²ΠΈΡ
ΡΠ° Π· ΠΎΡΠ³Π°Π½ΡΠ·Π°ΡΡΡ Π·Π°Ρ
ΠΈΡΡΡ. ΠΡΡΡΠ½ΡΠΎΠ²Π°Π½Ρ Π½Π° ΡΡΡΠ΄Π΅Π½ΡΡΠ² ΡΠ° ΠΊΠ΅ΡΡΠ²Π½ΠΈΠΊΡΠ²
ΠΊΠ²Π°Π»ΡΡΡΠΊΠ°ΡΡΠΉΠ½ΠΈΡ
ΡΠΎΠ±ΡΡ Π΄Π»Ρ ΠΎΡΠ³Π°Π½ΡΠ·Π°ΡΡΡ Ρ Π²ΠΈΠΊΠΎΠ½Π°Π½Π½Ρ Π·Π°Π²Π΄Π°Π½Ρ Π½Π°Π²ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΠ»Π°Π½Ρ
ΠΏΡΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ Π±Π°ΠΊΠ°Π»Π°Π²ΡΡΠ² ΡΠ° ΡΠΏΠ΅ΡΡΠ°Π»ΡΡΡΡΠ²
Π ΠΏΠ»Π°Π·ΠΌΠ΅Π½Π½ΠΎ-Π²ΠΈΠ±ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΌ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠΈ Π½Π° ΠΆΠ΅Π»Π΅Π·ΠΎΡΡΠ΄Π½ΡΠΉ ΠΌΠ°ΡΡΠΈΠ² Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ ΡΠ΅ΡΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΠΎΡΠ»ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ
Π ΠΎΠ·Π³Π»ΡΠ½ΡΡΡ ΠΏΡΠΎΡΠ΅ΡΠΈ ΠΎΠ±βΡΠΌΠ½ΠΎΡ ΡΠΎΠ½ΡΠ·Π°ΡΡΡ Π² ΠΊΠ²Π°ΡΡΠΈΡΠ°Ρ
, ΡΠΊΡ Π²ΠΈΠ½ΠΈΠΊΠ°ΡΡΡ ΠΏΡΠΈ ΠΏΠ»Π°Π·ΠΌΠΎΠ²ΠΎ-Π²ΡΠ±ΡΠΎΡΠ΄Π°ΡΠ½ΡΠΉ Π΄ΡΡ Π½Π° ΠΌΡΠ½Π΅ΡΠ°Π»ΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΠΎ ΠΏΠ»Π°Π·ΠΌΠΎΠ²ΠΎ Π²ΡΠ±ΡΠΎΡΠ΄Π°ΡΠ½Π΅ Π½Π°Π²Π°Π½ΡΠ°ΠΆΠ΅Π½Π½Ρ ΠΌΠ°ΡΠ΅ΡΡΠ°Π»ΡΠ² ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡΡ Π΄ΠΎ Π²ΠΈΠ½ΠΈΠΊΠ½Π΅Π½Π½Ρ Π»ΠΎΠΊΠ°Π»ΡΠ½ΠΈΡ
ΠΎΠ±Π»Π°ΡΡΠ΅ΠΉ Π· Π°Π½ΠΎΠΌΠ°Π»ΡΠ½ΠΈΠΌ Π΅ΡΠ΅ΠΊΡΠΎΠΌ Π¨ΠΎΡΡΠΊΠΈ.The process of volume ionization in the quartzite, that arose at plasma-vibrostriking action upon minerals, was considered. It was shown, that the plasma-vibrostriking loading of materials resulted in the appearance of local regions with an anomalous Shottci effect
Sclerodactyly and Diabetic Complications among Egyptian Adolescent Type 1 Diabetic Patient
BACKGROUND: One of the common complications of diabetic patients is sclerodactyly which is considered as a part of limited joint mobility.
AIM: To assess sclerodactyly in adolescent type 1 diabetics and to detect its relation to other diabetic complications.
METHODS: Sixty-three diabetics and 60 controls were studied. Clinical, laboratory assessment, ultrasonography of the skin, carotid artery intima-media thickness (cIMT) & renal colour duplex were done for all participants.
RESULTS: Sclerodactyly was positive in 12 (19%) of diabetics. Patients with sclerodactyly had a significantly thickened skin compared to patients without sclerodactyly and controls, P = 0.0001. Male diabetics had significantly thickened skin (p = 0.0001). Diabetic patients with sclerodactyly had significant higher systolic blood pressure (p = 0.03), cholesterol (p = 0.05) and triglyceride (p = 0.004) and lower HDL-c (p = 0.04). Skin thickness had a significant positive correlation with age of diabetic patients (p = 0.02), waist/height ratio (p = 0.04), glycosylated hemoglobin (p = 0.03), albumin/creatinine ratio (p = 0.03), and cIMT (p = 0.03).
CONCLUSION: Ultrasound easily diagnoses sclerodactyly. Diabetic patients had a high prevalence of sclerodactyly with increased macrovascular and microvascular complications. Sclerodactyly may be a marker for diabetic vascular complications. Frequent follow up of diabetic patients for early detection of sclerodactyly in uncontrolled diabetic patients is recommended. It could be an alarming sign for microalbuminuria, hypertension, hyperlipidaemia and atherosclerosis
ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠΈΡΡΠ΅ΠΌ Π±Π΅ΡΠΏΠ΅ΡΠ΅Π±ΠΎΠΉΠ½ΠΎΠ³ΠΎ ΡΠ»Π΅ΠΊΡΡΠΎΠΏΠΈΡΠ°Π½ΠΈΡ Π² ΡΠΊΡΡΡΠ΅Π½Π½ΠΎΠΉ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½Π΅
Π Π°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΠΏΠΎΡΡΡΠΎΠ΅Π½ΠΈΡ ΡΠΈΡΡΠ΅ΠΌ Π±Π΅ΡΠΏΠ΅ΡΠ΅Π±ΠΎΠΉΠ½ΠΎΠ³ΠΎ ΡΠ»Π΅ΠΊΡΡΠΎΠΏΠΈΡΠ°Π½ΠΈΡ ΡΠΊΡΡΡΠ΅Π½Π½ΡΡ
ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΡ
ΠΎΡΠ΄Π΅Π»Π΅Π½ΠΈΠΉ Π½Π° Π±Π°Π·Π΅ ΠΏΡΠ΅ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°ΡΠ΅Π»Π΅ΠΉ Π½Π°ΠΏΡΡΠΆΠ΅Π½ΠΈΡ. ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ ΡΡ
Π΅ΠΌΡ ΡΠΈΡΡΠ΅ΠΌ Π±Π΅ΡΠΏΠ΅ΡΠ΅Π±ΠΎΠΉΠ½ΠΎΠ³ΠΎ ΡΠ»Π΅ΠΊΡΡΠΎΠΏΠΈΡΠ°Π½ΠΈΡ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΎΡΡΠΈΠ»Π»ΠΎΠ³ΡΠ°ΠΌΠΌΡ ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄Π½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² Π²ΡΡ
ΠΎΠ΄Π½ΠΎΠ³ΠΎ Π½Π°ΠΏΡΡΠΆΠ΅Π½ΠΈΡ ΠΏΡΠΈ ΡΠ°Π±ΠΎΡΠ΅ ΡΠΈΡΡΠ΅ΠΌΡ Π½Π° ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ ΠΏΠ°ΡΠ°Π»Π»Π΅Π»ΡΠ½ΠΎ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½Π½ΡΡ
ΡΠ°Π·Π½ΠΎΡΠΎΠ΄Π½ΡΡ
Π½Π°Π³ΡΡΠ·ΠΎΠΊ
ΠΡΠΎΠ΅ΠΊΡ ΡΡΡΠ°Π½ΠΎΠ²ΠΊΠΈ ΠΎΡΠΌΡΠ²ΠΊΠΈ ΡΠ»Π΅ΠΊΡΡΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΡΠΎΡΠΊΠ° ΡΠΈΡΠ°Π½Π° ΠΎΡ ΡΡΠΎΡΠΈΠ΄Π½ΡΡ ΡΠΎΠ»Π΅ΠΉ ΡΠ»Π΅ΠΊΡΡΠΎΠ»ΠΈΡΠ° ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ 30 ΠΊΠ³/ΡΠ°Ρ
ΠΡΠΏΡΡΠΊΠ½Π°Ρ ΠΊΠ²Π°Π»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½Π°Ρ ΡΠ°Π±ΠΎΡΠ° Π² ΡΠΎΡΠΌΠ΅ Π΄ΠΈΠΏΠ»ΠΎΠΌΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠ΅ΠΊΡΠ° 118 Ρ., 24 ΡΠΈΡ., 23 ΡΠ°Π±Π»., 46 ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΎΠ², 1 ΠΏΡΠΈΠ»ΠΎΠΆΠ΅Π½ΠΈΠ΅.
ΠΠ»ΡΡΠ΅Π²ΡΠ΅ ΡΠ»ΠΎΠ²Π°: ΡΠ»Π΅ΠΊΡΡΠΎΠ»ΠΈΠ·, ΠΊΠ°ΡΠΎΠ΄Π½ΡΠΉ ΠΎΡΠ°Π΄ΠΎΠΊ, ΠΎΡΠΌΡΠ²ΠΊΠ°.
Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ β ΡΠΏΡΠΎΠ΅ΠΊΡΠΈΡΠΎΠ²Π°ΡΡ ΡΠ΅Ρ
Π΄Π»Ρ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΎΡΠΌΡΠ²ΠΊΠΈ ΡΠΈΡΠ°Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΡΠΎΡΠΊΠ°.
ΠΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠ²Π½ΡΠ΅, ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈ ΡΠ΅Ρ
Π½ΠΈΠΊΠΎ-ΡΠΊΡΠΏΠ»ΡΠ°ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ: ΠΠ±ΡΠ΅ΠΌ ΠΈ Π³Π°Π±Π°ΡΠΈΡΠ½ΡΠ΅ ΡΠ°Π·ΠΌΠ΅ΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠ³ΠΎ Π°ΠΏΠΏΠ°ΡΠ°ΡΠ° ΡΠΎΡΡΠ°Π²Π»ΡΡΡ: V = 0,25 ΠΌ3, D = 0,8 ΠΌ, H = 1,8 ΠΌ.
Π‘ΡΠ΅ΠΏΠ΅Π½Ρ Π²Π½Π΅Π΄ΡΠ΅Π½ΠΈΡ: ΠΏΡΠΎΠ΅ΠΊΡ Π½Π°Ρ
ΠΎΠ΄ΠΈΡΡΡ Π½Π° ΡΡΠ°Π΄ΠΈΠΈ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ.
ΠΠ±Π»Π°ΡΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ: Π₯ΠΈΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡ ΡΠ΅Π΄ΠΊΠΈΡ
ΠΌΠ΅ΡΠ°Π»Π»ΠΎΠ².
ΠΠΊΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ/Π·Π½Π°ΡΠΈΠΌΠΎΡΡΡ ΡΠ°Π±ΠΎΡΡ: ΡΠΊΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΡΠ΅ΠΊΡ β ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΠ΅Π±Π΅ΡΡΠΎΠΈΠΌΠΎΡΡΠΈ ΡΠΈΡΠ°Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΡΠΎΡΠΊΠ° ΠΈ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠΈΠ±ΡΠ»ΠΈ.Die AbschluΓqualifikationsarbeit in Form vom Diplomprojekt 118 mit., 24 Abb., 23 Tabellen, 46 Quellen, 1 Anlage.
Die StichwΓΆrter: die Elektrolyse, die Kathodenablagerung, das Abwaschen.
Das Ziel der Arbeit β die Abteilung fΓΌr die Verwirklichung des Prozesses des Abwaschens des Titanpulvers zu entwerfen.
Die Haupt- konstruktiven, technologischen und techniko-Betriebscharakteristiken: der Umfang und die LadeumfΓ€nge des Hauptapparates bilden: V = 0,25 ΠΌ3, D = 0,8 m, H = 1,8 m
Die Stufe der EinfΓΌhrung: das Projekt befindet sich auf dem Stadium der Entwicklung.
Das Gebiet der Anwendung: die Chemische Technologie der seltenen Metalle.
Die WirtschaftseffektivitΓ€t/Bedeutsamkeit der Arbeit: der Nutzeffekt β die Selbstkostenverringerung des Titanpulvers und die VergrΓΆΓerung des Gewinnes
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