39 research outputs found
American Adolescents at Work
Despite advances in technology and medicine, safety for working adolescents still challenges 21st century Americans. One would think that by now, in the beginning of the new millennium, America would have cured this disease of child labor that infects its younger population. Yet, injuries still maim and kill America's working youth. Politicians speak out against child obesity, and both celebrities and ordinary citizens criticize school violence, especially after a Columbine or Virginia Tech massacre. Human rights activists picket clothing lines that depend upon the work of underpaid children in developing countries, and Congress holds hearings to ensure that American consumers do not buy goods produced by these exploited children. However, health care providers, legislators, and the general public often relegate child labor to the back burner. Moreover, many diminish the role of child labor in the United States by viewing child labor as a social, economic, and political problem limited to developing countries. The employment of children in the work force should be in the forefront of domestic health policy because of its social and economic significance to public health. Even though current societal awareness indicates some understanding of the health risks of adolescent workers, statistics continue to show a bleak picture of preventable workplace injuries and fatalities of this vulnerable population.This paper defines "child or youth" as any individual 17 or younger who engages in some kind of work. In discussing youth employment, the paper does more than just describe child labor laws; it also focuses on the unique traits of this young population and the trends that characterize its employment. This gives an identity to the faceless young men and women who deal with the risks of the industrial and agricultural work places. Once presenting the current statistics on injuries and fatalities incurred by youth in both the industrial and agricultural sectors, the paper compares the similarities and differences in the major industries between youth and adult workers. It then moves into the legal arena, describing what has been done and what still needs to happen to combat child labor problems
A role for Syk-kinase in the control of the binding cycle of the β2 integrins (CD11/CD18) in human polymorphonuclear neutrophils
A fine control of β2 integrin (CD11/CD18)-mediated firm adhesion of human neutrophils to the endothelial cell monolayer is required to allow ordered emigration. To elucidate the molecular mechanisms that control this process, intracellular protein tyrosine signaling subsequent to β2 integrin-mediated ligand binding was studied by immunoprecipitation and Western blotting techniques. The 72-kDa Syk-kinase, which was tyrosine-phosphorylated upon adhesion, was found to coprecipitate with CD18, the β-subunit of the β2 integrins. Moreover, inhibition of Syk-kinase by piceatannol enhanced adhesion and spreading but diminished N-formyl-Met-Leu-Phe-induced chemotactic migration. The enhancement of adhesiveness was associated with integrin clustering, which results in increased integrin avidity. In contrast, piceatannol had no effect on the surface expression or on the affinity of β2 integrins. Altogether, this suggests that Syk-kinase controls alternation of β2 integrin-mediated ligand binding with integrin detachment
Growth performance, nutrients digestibility, and blood metabolites of lambs fed diets supplemented with probiotics during pre- and post-weaning period
Go6976 abrogates GPVI-mediated platelet functional responses in human platelets through inhibition of Syk
Negative Regulation of FcϵRI-mediated Signaling and Mast Cell Function by the Adaptor Protein LAX
Phosphatidylinositol-3,4,5-trisphosphate (PtdIns-3,4,5-P3)/Tec kinase-dependent calcium signaling pathway: a target for SHIP-mediated inhibitory signals.
Tec family non-receptor tyrosine kinases have been implicated in signal transduction events initiated by cell surface receptors from a broad range of cell types, including an essential role in B-cell development. A unique feature of several Tec members among known tyrosine kinases is the presence of an N-terminal pleckstrin homology (PH) domain. We directly demonstrate that phosphatidylinositol-3,4,5-trisphosphate (PtdIns-3,4,5-P3) interacting with the PH domain acts as an upstream activation signal for Tec kinases, resulting in Tec kinase-dependent phospholipase Cgamma (PLCgamma) tyrosine phosphorylation and inositol trisphosphate production. In addition, we show that this pathway is blocked when an SH2-containing inositol phosphatase (SHIP)-dependent inhibitory receptor is engaged. Together, our results suggest a general mechanism whereby PtdIns-3,4,5-P3 regulates receptor-dependent calcium signals through the function of Tec kinases
The novel Syk inhibitor R406 reveals mechanistic differences in the initiation of GPVI and CLEC-2 signaling in platelets
Focal adhesion proteins connect IgE receptors to the cytoskeleton as revealed by micropatterned ligand arrays
Patterned surfaces that present specific ligands in spatially defined arrays are used to examine structural linkages between clustered IgE receptors (IgE-FcεRI) and the cytoskeleton in rat basophilic leukemia (RBL) mast cells. We showed with fluorescence microscopy that cytoskeletal F-actin concentrates in the same regions as cell surface IgE-FcεRI that bind to the micrometer-size patterned ligands. However, the proteins mediating these cytoskeletal connections and their functional relevance were not known. We now show that whereas the adaptor proteins ezrin and moesin do not detectably concentrate with the array of clustered IgE-FcεRI, focal adhesion proteins vinculin, paxillin, and talin, which are known to link F-actin with integrins, accumulate in these regions on the same time scale as F-actin. Moreover, colocalization of these focal adhesion proteins with clustered IgE-FcεRI is enhanced after addition of fibronectin-RGD peptides. Significantly, the most prominent rat basophilic leukemia cell integrin (α5) avoids the patterned regions occupied by the ligands and associates preferentially with exposed regions of the silicon substrate. Thus, spatial separation provided by the patterned surface reveals that particular focal adhesion proteins, which connect to the actin cytoskeleton, associate with ligand-cross-linked IgE-FcεRI, independently of integrins. We investigated the functional role of one of these proteins, paxillin, in IgE-FcεRI-mediated signaling by using small interfering RNA. From these results, we determine that paxillin reduces stimulated phosphorylation of the FcεRI β subunit but enhances stimulated Ca2+ release from intracellular stores. The results suggest that paxillin associated with clustered IgE-FcεRI has a net positive effect on FcεRI signaling