Operation of a concentrated mode dual-alkali scrubber plant at the Lonmin smelter

Lonmin Platinum installed a concentrated mode dual-alkali scrubber at the smelter in Marikana in 2002. The dual-alkali scrubber was the technology of choice at that time as a sulphur fixation plant, due to the perceived benefits of handling both the high SO2 concentrations of converter off-gas and the swings in SO2 concentration that are linked to Peirce-Smith operation. Owing to current and impending legislative requirements for air quality and waste, Lonmin is currently considering additions to the dual-alkali plant, but is also evaluating alternative technologies for sulphur fixation. This paper reviews the decision of Lonmin to install a concentrated mode dual-alkali scrubber and presents plant performance achieved. It also describes the important control variables and sensitivities of the plant, and the final product that is produced by the operation of the plant. The legislative requirement that drives the Lonmin technology evaluation is also discussed

Water and radiation use efficiency of sugarcane for bioethanol production in South Africa, benchmarked against other selected crops

There are indications that high-fibre sugarcane genotypes may produce more biomass and use resources more efficiently than conventional sugarcane cultivars. The objective of this research was to gather quantitative information on resource use for selected conventional and high-fibre sugarcane genotypes and benchmark it against other bioethanol crops. Although conventional sugarcane initially grew slower than sorghum and Napier grass, it produced very high biomass (about 70 t ha−1) and theoretical ethanol (first- and secondgenerations) yields (about 27 kL ha−1) at 12 months, and used water relatively efficiently (about 5 kg m−3 and 2 kL m−3), out-performing all other crops except sorghum. The contribution of cellulosic ethanol to total ethanol yield varied hugely, from 89% for the high-fibre sugarcane hybrid to about 48% for conventional sugarcane, to as low as 14% for sugar beet. The high-fibre sugarcane hybrid grew faster initially and produced more biomass at eight months (56 t ha−1 vs 45 t ha−1) than the conventional types, but then flowered, reducing its growth rates markedly thereafter. It was also less sensitive to mild drought conditions. The results suggest that cellulosic ethanol production may be a feasible option that could be incorporated into conventional or biomass sugarcane production systems.South African Sugarcane Research Institute.http://www.tandfonline.com/loi/tjps202017-01-30hb201

Isokinetic neck strength norms for schoolboy rugby forwards

Objective. To generate isokinetic neck strength norms for schoolboy rugby forwards. Design. Two hundred and eight schoolboys (17.21 – 1.03 years, mean – standard error of the mean (SEM), chosen from a population of under-19 first and second XV rugby players, participated in this study. The subjects were assessed anthropometrically and isokinetically according to a set protocol. The isokinetic assessment of neck strength was performed with the use of a specially designed stabilising chair and halo. The subjects performed a single maximal exertion set, consisting of 3 repetitions, through each of the cervical spinal movements in the sagittal and frontal planes. The data were analysed statistically according to positional categories (front-, second-, and back-row forwards), and were used to generate Stanine tables of normative data concerning the force characteristics of the cervical spine. Results. The front-row forwards produced the largest amounts of force during the measurement of peak torque flexion (PTF = 30.00 – 1.39 Nm) and peak torque extension (PTE = 55.26 – 1.42 Nm). Conversely, the second-row forwards performed the best during the measurement of lateral flexion peak torque to the right (PTR = 53.71 – 1.51 Nm) and lateral flexion peak torque to the left (PTL = 52.92 – 1.63 Nm) in the frontal plane. The front-row forwards were the most powerful in all the neck movements measured (power generated at 0.2 seconds during flexion (PowF) = 101.54 – 6.43 W, power generated at 0.2 s during extension (PowE) = 167.31 – 8.03 W, power generated at 0.2 s during lateral flexion to the right (PowR) = 211.92 – 7.44 W, and power generated at 0.2 s during lateral flexion to the left (PowL) = 194.81 – 7.73 W). However, further analysis of the data revealed that few statistically significant differences (p < 0.01 and p < 0.05) existed between the positional categories for the measured variables of peak torque, power generated at 0.2 of a second, peak torque to body mass ratio and cervical range of motion. Conclusion. It appears that the various positional categories have not undergone the expected neck strength adaptations to meet the unique requirements of each position. The generation of neck strength normative data allows for the effective and quantified comparison of neck strength variables, enabling more effective injury prevention and rehabilitation. South African Sports Medicine Vol.17(1) 2005: 19-2

Structure-activity relationships of the antimicrobial peptide arasin 1 - and mode of action studies of the N terminal, proline-rich region

Arasin 1 is a 37 amino acid long proline-rich antimicrobial peptide isolated from the spider crab, Hyas araneus. In this work the active region of arasin 1 was identified through structure-activity studies using different peptide fragments derived from the arasin 1 sequence. The pharmacophore was found to be located in the proline/arginine-rich NH2 terminus of the peptide and the fragment arasin 1(1–23) was almost equally active to the full length peptide. Arasin 1 and its active fragment arasin 1(1–23) were shown to be non-toxic to human red blood cells and arasin 1(1–23) was able to bind chitin, a component of fungal cell walls and the crustacean shell. The mode of action of the fully active N-terminal arasin 1(1–23) was explored through killing kinetic and membrane permeabilization studies. At the minimal inhibitory concentration (MIC), arasin 1(1–23) was not bactericidal and had no membrane disruptive effect. In contrast, at concentrations of 5×MIC and above it was bactericidal and interfered with membrane integrity. We conclude that arasin 1(1–23) has a different mode of action than lytic peptides, like cecropin P1. Thus, we suggest a dual mode of action for arasin 1(1–23) involving membrane disruption at peptide concentrations above MIC, and an alternative mechanism of action, possibly involving intracellular targets, at MIC

Loss of tolerance to gut immunity protein; glycoprotein 2 (GP2) is associated with progressive disease course in primary sclerosing cholangitis

Abstract Glycoprotein 2[GP2] is a specific target of pancreatic autoantibodies[PAbs] in Crohn’s disease(CD) and is involved in gut innate immunity processes. Our aim was to evaluate the prevalence and prognostic potential of PAbs in primary sclerosing cholangitis(PSC). Sixty-five PSC patients were tested for PAbs by indirect immunofluorescence and compared with healthy (n = 100) and chronic liver disease controls(CLD, n = 488). Additionally, a panel of anti-microbial antibodies and secretory (s)IgA levels were measured, as markers of bacterial translocation and immune dysregulation. PAbs were more frequent in PSC(46.2%) compared to controls(healthy:0% and CLD:4.5%), [P < 0.001, for each]. Occurrence of anti-GP2 antibody was 30.8% (20/65) and was exclusively of IgA isotype. Anti-GP2 IgA positive patients had higher sIgA levels (P = 0.021). With flow-cytometry, 68.4% (13/19) of anti-GP2 IgA antibodies were bound with secretory component, suggesting an active retro-transportation of anti-GP2 from the gut lumen to the mucosa. Anti-GP2 IgA was associated with shorter transplant-free survival [PLogRank < 0.01] during the prospective follow-up (median, IQR: 87 [9–99] months) and remained an independent predictor after adjusting for Mayo risk score(HR: 4.69 [1.05–21.04], P = 0.043). These results highlight the significance of gut-liver interactions in PSC. Anti-GP2 IgA might be a valuable tool for risk stratification in PSC and considered as a potential therapeutic target

B Lymphocyte intestinal homing in inflammatory bowel disease

<p>Abstract</p> <p>Background</p> <p>Inflammatory bowel disease (IBD) is thought to be due to an abnormal interaction between the host immune system and commensal microflora. Within the intestinal immune system, B cells produce physiologically natural antibodies but pathologically atypical anti-neutrophil antibodies (xANCAs) are frequently observed in patients with IBD. The objective is to investigate the localisation of immunoglobulin-producing cells (IPCs) in samples of inflamed intestinal tissue taken from patients with IBD, and their possible relationship with clinical features.</p> <p>Methods</p> <p>The IPCs in small intestinal, colonic and rectal biopsy specimens of patients with IBD were analysed by means of immunofluorescence using polyclonal rabbit anti-human Ig and goat anti-human IgM. The B cell phenotype of the IPC-positive samples was assessed using monoclonal antibodies specific for CD79, CD20, CD23, CD21, CD5, λ and κ chains. Statistical correlations were sought between the histological findings and clinical expression.</p> <p>Results</p> <p>The study involved 96 patients (64 with ulcerative colitis and 32 with Crohn's disease). Two different patterns of B lymphocyte infiltrates were found in the intestinal tissue: one was characterised by a strong to moderate stromal localisation of small IgM⁺/CD79⁺/CD20^-/CD21^-/CD23^-/CD5^±IPCs (42.7% of cases); in the other (57.3%) no such small IPCs were detected in stromal or epithelial tissues. <it>IPCs </it>were significantly less frequent in the patients with Crohn's disease than in those with ulcerative colitis (p = 0.004).</p> <p>Conclusion</p> <p>Our findings suggest that different immunopathogenetic pathways underlie chronic intestinal inflammation with different clinical expressions. The presence of small B lymphocytes resembling B-1 cells also seemed to be negatively associated with Crohn's disease. It can therefore be inferred that the gut contains an alternative population of B cells that have a regulatory function.</p

The immunobiology of primary sclerosing cholangitis

Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease histologically characterized by the presence of intrahepatic and/or extrahepatic biliary duct concentric, obliterative fibrosis, eventually leading to cirrhosis. Approximately 75% of patients with PSC have inflammatory bowel disease. The male predominance of PSC, the lack of a defined, pathogenic autoantigen, and the potential role of the innate immune system suggest that it may be due to dysregulation of immunity rather than a classic autoimmune disease. However, PSC is associated with several classic autoimmune diseases, and the strongest genetic link to PSC identified to date is with the human leukocyte antigen DRB01*03 haplotype. The precise immunopathogenesis of PSC is largely unknown but likely involves activation of the innate immune system by bacterial components delivered to the liver via the portal vein. Induction of adhesion molecules and chemokines leads to the recruitment of intestinal lymphocytes. Bile duct injury results from the sustained inflammation and production of inflammatory cytokines. Biliary strictures may cause further damage as a result of bile stasis and recurrent secondary bacterial cholangitis. Currently, there is no effective therapy for PSC and developing a rational therapeutic strategy demands a better understanding of the disease

Lymphocyte recruitment and homing to the liver in primary biliary cirrhosis and primary sclerosing cholangitis

The mechanisms operating in lymphocyte recruitment and homing to liver are reviewed. A literature review was performed on primary biliary cirrhosis (PBC), progressive sclerosing cholangitis (PSC), and homing mechanisms; a total of 130 papers were selected for discussion. Available data suggest that in addition to a specific role for CCL25 in PSC, the CC chemokines CCL21 and CCL28 and the CXC chemokines CXCL9 and CXCL10 are involved in the recruitment of T lymphocytes into the portal tract in PBC and PSC. Once entering the liver, lymphocytes localize to bile duct and retain by the combinatorial or sequential action of CXCL12, CXCL16, CX3CL1, and CCL28 and possibly CXCL9 and CXCL10. The relative importance of these chemokines in the recruitment or the retention of lymphocytes around the bile ducts remains unclear. The available data remain limited but underscore the importance of recruitment and homing