Creative Interventions to Increase Counselor-in-Training Wellness

Wellness is a broad term that emphasizes a person’s current state of mental, physical, emotional, spiritual, and professional wellness. Professional counseling was built upon the foundations of wellness and practitioner self-care. While counseling programs understand the importance of wellness and counseling ethics emphasize the necessity of professional wellness, students frequently do not feel prepared or trained enough in wellness dimensions. This manuscript provides a rationale for a program wellness model and outlines a proposed series of interventions aimed at increasing students’ understanding, ability to self-assess, and strategies related to self-care and wellness

Cell Type–Specific Transcriptome Analysis Reveals a Major Role for Zeb1 and miR-200b in Mouse Inner Ear Morphogenesis

Cellular heterogeneity hinders the extraction of functionally significant results and inference of regulatory networks from wide-scale expression profiles of complex mammalian organs. The mammalian inner ear consists of the auditory and vestibular systems that are each composed of hair cells, supporting cells, neurons, mesenchymal cells, other epithelial cells, and blood vessels. We developed a novel protocol to sort auditory and vestibular tissues of newborn mouse inner ears into their major cellular components. Transcriptome profiling of the sorted cells identified cell type–specific expression clusters. Computational analysis detected transcription factors and microRNAs that play key roles in determining cell identity in the inner ear. Specifically, our analysis revealed the role of the Zeb1/miR-200b pathway in establishing epithelial and mesenchymal identity in the inner ear. Furthermore, we detected a misregulation of the ZEB1 pathway in the inner ear of Twirler mice, which manifest, among other phenotypes, malformations of the auditory and vestibular labyrinth. The association of misregulation of the ZEB1/miR-200b pathway with auditory and vestibular defects in the Twirler mutant mice uncovers a novel mechanism underlying deafness and balance disorders. Our approach can be employed to decipher additional complex regulatory networks underlying other hearing and balance mouse mutants

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN

Street tree density and distribution: an international comparative analysis of five cities

Cities around the world are demonstrating significant interest in urban greening to realize a range of socioecological benefits. The urban greening toolkit often includes street trees, an essential component of urban design informed by historic legacies of both human and environmental factors. To date, there has been little comparative analysis of street tree density and distribution across international and intercontinental settings, and associated research has not been situated within the broader discussion on historical legacies. This study focuses on five capital cities (Buenos Aires, Ottawa, Paris, Stockholm and Washington, D.C.) situated in two climate zones and it addresses two research questions: (1) what are the density and distribution of street trees across a given city and its street hierarchy? (2) how do these metrics compare within and between cities by climate zone? The analysis draws upon up-to-date datasets from local authorities and includes geospatial analysis of street trees across hierarchical street classes within the central zones of each city. The results show clear differences in street tree density in cities within and between climate zones as well as differences in street tree distribution in cities within the same climate zone. Substantial differences within climate zones further suggests that cultural factors–including but not limited to urban form, aesthetic norms, and governance regimes–may play a pivotal role in the distribution and density of street trees. This illustrates the importance of place-specific cultural and environmental legacies as determinants of street tree density and distribution and supports further comparative research on the topic

Beyond ‘trees are good’: Disservices, management costs, and tradeoffs in urban forestry

The provision of ecosystem services is a prominent rationale for urban greening, and there is a prevailing mantra that ‘trees are good’. However, understanding how urban trees contribute to sustainability must also consider disservices. In this perspective article, we discuss recent research on ecosystem disservices of urban trees, including infrastructure conflicts, health and safety impacts, aesthetic issues, and environmentally detrimental consequences, as well as management costs related to ecological disturbances and risk management. We also discuss tradeoffs regarding species selection and local conservation concerns, as well as the central role of human perception in the interpretation of ecosystem services and disservices, particularly the uncritical assertion that ‘everybody loves trees’. Urban forestry decision-making that fails to account for disservices can have unintended negative consequences for communities. Further research is needed regarding life cycle assessments, stakeholder decision-making, return-on-investment, and framings of services and disservices in urban forestry