Chronic respiratory pathology and life quality of the adolescents living in industrial centers

At present the pathology of laryngeal and respiratory organs is known to prevail in the structure of the child and adolescent morbidity in the industrial centers of the northern and eastern regions of the country and their proportion reaches 70 %. The disease symptoms of the respiratory tracts (the obstacle nose, discomfort and throat tickling as well as the hoarseness) and especially their complications may lead to the decrease in the life quality of children and adolescents. Taking into account that at present time it is impossible to remove technogenic air pollution totally, the complex studies on revealing the unfavorable factors, which may influence the organisms of the children and adolescents, are known to be the prior task of the preventive medicine. The aim of the study was to examine the frequency chronic respiratory pathology and influence on the adolescents life quality of industrial centers of Irkutsk region with different levels of technogenic pollution. 410 adolescents (14-17 years) for a long time living in the regions with the different air pollution levels air with the developed oil-chemical and industries were examined. The differences in the structure of chronic respiratory pathology were revealed in the examined adolescents: under conditions of the air pollution with a complex of chemical compounds with a high Hasard Index (HI = 17) the respiratory diseases prevail, and with the medium level (HI = 3,6) the nasal diseases prevail. It was found that that about 12,6 % of the morbidity variability in the category of the respiratory organs, in particular the throat pathology in the teenagers may be correlated with the air pollution. The more significant factors of forming the chronic respiratory pathology were found to be the health state, characterized by the laboratory indices (common microbial number of the nasal cavity and the pharynx; activity of lysocyme); chemical inhalation exposure; the work of the parents under harmful conditions during the period before child birth. The study results may be considered as the basis for the optimization of the approaches to the diagnostics and prevention of the respiratory pathology of adolescents

Official and Business Styles’ Presentation of Genres in the Education Text

Статья посвящена методическим вопросам ознакомления студентов вуза с жанрами документов в процессе преподавания документной лингвистики. Авторы считают, что организация учебного процесса в той его части, которая посвящена типологии письменных жанров официально-делового стиля, может быть сопряжена с научной работой студентов. Рассматриваются основные проблемы изучения жанровой специфики документов и пути их решения в процессе освоения предмета «Документная лингвистика».The article is devoted to the methodological issues of higher school students’ familiarization with the genres of documents in the teaching of documentary linguistics. The authors believe that the organization of the educational process in that part of it, which is devoted to the typology of official business style’s written genres, can be associated with the students’ scientific work. The main problems of studying the genre specificity of documents and ways to solve them in the process of mastering the subject “Documentary linguistics” are considered

Electronic-microscopic estimation of changes in erythrocytes ultrastructure under influence of low-frequency ultrasound

The paper presents the estimation of changes of erythrocytes ultra-structure under influence of low-frequency ultrasound using various types of waveguides. It was demonstrated that application of ultrasound contributes to change of erythrocyte form and size, which depend on intensity, duration and type of the waveguide

Bioinformatic Analysis of Immunodominant Peptides of Rabies Virus (<i>Rabies lyssavirus, Rhabdoviridae</i>)

There is a need to develop a new generation of anti-rabies vaccines that provide a protective level of antibodies after a single injection. Prospects for solving this problem are opened by the latest developments in the field of “reverse vaccinology”. The main parameter that determines the effectiveness of recombinant vaccines is the design of the antigen-coding sequence. In this regard, the aim of the work was to conduct a bioinformatic analysis of rabies virus (Rabies lyssavirus, Rhabdoviridae) peptides to identify immunogenic epitopes.Materials and methods. Analysis of 5 candidate protein sequences of more than 100 strains and epizootic isolates of the rabies virus was performed using standard in silico prediction methods using Immune Epitope Database (IEDB) (NIH, USA).Results and discussion. As a result of the analysis of primary amino acid sequences, carried out using the most commonly used bioinformatics tools, the number of immunogenic epitopes and the types of immune response detected (T- and B-cell epitopes, class I MHCbinding epitopes) were established for viral proteins: glycoprotein (G), nucleoprotein (N), phosphoprotein (P), matrix protein (M), RNA-dependent RNA polymerase (L). In the amino acid structure of these proteins, N- and O-glycosylation sites, signal peptides, and transmembrane domains were additionally identified. In order to predict the safety and efficacy of these proteins as components of recombinant vaccines, an in silico assessment of their physicochemical properties was carried out. Despite the fact that the predominant number of epitopes is concentrated in the structure of the glycoprotein, the epitopes of other proteins, ranging according to the level of antigenicity and conservatism, may also be of interest as components of preventive drugs or diagnostics. The presented data can be used in the design of the insert during the construction of a candidate virus-vector vaccine or control positive samples in diagnostic methods based on the indication of viral genome fragments

Frequency of hyperglycemia and polymorphism of TNF and TP53 genes in patients with acute pancreatitis, chronic pancreatitis, pancreatic cancer

BACKGROUND: «The vicious circle» of associations of diabetes mellitus (DM) with pancreatic pathology, when pancreatic diseases can initiate DM, and type 2 DM — cause functional and organic pancreatic pathology, determines the search for possible associations. Some studies have established a relationship between TNF or TP53 polymorphisms with DM or with pancreatic diseases.AIMS: to determine and compare fasting plasma glucose and the frequency of hyperglycemia in patients with acute pancreatitis (APp), chronic pancreatitis (CPp), pancreatic cancer (PCp) depending on gender, etiology or stage of the disease, polymorphism -308G/A TNF gene in all patients, and polymorphism 72Arg/Pro gene TP53 in PCp..MATERIALS AND METHODS: At the observational multicenter clinical cross-sectional uncontrolled case-study 44 APp, 97 CPp and 45 PCp were examined; the groups were comparable by sex/age. Informed consent form for participate in the study was obtained from all patients. The main outcome of the study: frequency of hyperglycemia in APp, CPp, PCp, considering the polymorphism TNF and TP53 genes. RESULTS: The lowest age-standardized fasting plasma glucose (FPG) was found in CPp (6,2±0,2 mmol/l) than in APp (6,7±0,2 mmol/l, p=0,041). In PCp (6,6±0,2 mmol/l), the average levels of FPG did not differ substantially when compared with APp (p=0,749) or CPp (p=0,092). In APp, the norm of GP was detected less frequently (31,8%) than in CPp (54,6%, χ2 =6,3, p=0,012), and the frequency of the norm of GP in PCp (48,9%) did not differ with that in APp or CPp. The frequency of FPG≥6,1&lt;7,0 mmol/l did not differ in APp (20,5%), CPp (9,3%) or PCp (17,8%). The frequency of FGP≥7.0 mmol/l did not differ in APp CPp and PCp: 47,7, 36,1, 33,3%. Logistic regression analysis revealed a tendency for an increased chance of having stage 3–4 PC with FPG≥7,0 mmol/l (Exp (B)=3,205 95%CI 0,866–11,855, p=0,081) in PCp, but not in patients with pancreatic necrosis or “definite» СP.The frequencies of G/G (71,4, 74,7, 76,2%), G/A (26,2, 24,1, 23,8%) of TNF genotypes did not differ in APp, CPp or PCp, p&gt;0,05. In PCp genotypes Arg/Arg, Arg/Pro, Pro/Pro polymorphism gene 72Arg/Pro TP53 in 2,4, 35,7, 61,9% of cases. No associations of GP≥7,0 mmol/l with TNF polymorphism in APp, CPp, PCp and with TP53 polymorphism in PCp were obtained.CONCLUSIONS: The frequency of FGP≥7,0 mmol/l did not differ for various pancreatic disease and was not associated with the risk of pancreatic necrosis and “defined” CP. The -308G/A polymorphism TNF gene did not differ in APp, CPp or PCp and was not associated with impaired carbohydrate metabolism. The 72Arg/Pro polymorphism TP53 gene in PCp was not associated with impaired carbohydrate metabolism

Gastric Microbiota and Morphological Changes of the Gastroduodenal Tract Associated with Helicobacter Pylori Infection

© 2016, Springer Science+Business Media New York.Investigation of the microbiota at morphological changes of a gastrointestinal tract. One hundred four patients were examined by cytologic, bacteriological methods, and mass-spectrometry techniques. Studying of the microbial association of stomach has demonstrated the mixed microflora presented by Helicobacter pylori, cocci, fungi Candida, rods, and protozoa. Bacteria of the following species were found: Actinomyces, Arthrobacter, Bacillus, Corynebacterium, Lactobacillus, Neisseria, Pseudomonas, Rothia, Staphylococcus, Streptococcus, and Streptomyces. The microbial flora of esophageal samples was less various (opportunistic bacteria Neisseria, Gemella, and Rothia mucilaginosa were revealed). In group of Helicobacter-positive patients, bacteria were found in fungi Candida, Colletotrichum, and bacterias Thauera and Mycoplasma, while in H.pylori-negative patients, samples have more different species. Intensity of morphological changes (the atrophy, the intestinal metaplasia, and the dysplasia) correlated with a frequency of detection of H. pylori and eukaryotic microbiota (fungi Candida and protozoa)

4.各部門の活動内容 理学療法科

© 2016, Springer Science+Business Media New York.Investigation of the microbiota at morphological changes of a gastrointestinal tract. One hundred four patients were examined by cytologic, bacteriological methods, and mass-spectrometry techniques. Studying of the microbial association of stomach has demonstrated the mixed microflora presented by Helicobacter pylori, cocci, fungi Candida, rods, and protozoa. Bacteria of the following species were found: Actinomyces, Arthrobacter, Bacillus, Corynebacterium, Lactobacillus, Neisseria, Pseudomonas, Rothia, Staphylococcus, Streptococcus, and Streptomyces. The microbial flora of esophageal samples was less various (opportunistic bacteria Neisseria, Gemella, and Rothia mucilaginosa were revealed). In group of Helicobacter-positive patients, bacteria were found in fungi Candida, Colletotrichum, and bacterias Thauera and Mycoplasma, while in H.pylori-negative patients, samples have more different species. Intensity of morphological changes (the atrophy, the intestinal metaplasia, and the dysplasia) correlated with a frequency of detection of H. pylori and eukaryotic microbiota (fungi Candida and protozoa)

SAFETY ASSESSMENT OF FOOD-STUFF RAW MATERIAL AND FOOD PRODUCTS IN BURYAT REPUBLIC

The aim of study was a hygienic assessment on sanitary and chemical, microbiological indicators of food and the food staples, realized in the territory of the Buryat Republic during 2008-2012. Preparation for processing of primary data and the subsequent statistical analysis made with Excel and the integrated package STATISTICA 6.0. Calculation of percent of tests of hygienic standards not conforming to requirements (%) on areas and as a whole on the republic is carried out. The specified sizes are determined as for separate years, and on the average for the period. To average values 95% confidential intervals (CI) are calculated. As significant deviations from the average levels the sizes which are going out of CI were considered. The performed result of assessment of the laboratory studies may testify that the stable high percent of the samples which don't accord to the normative in the sanitary-chemical indices is available at present. Using the cluster analyses allowed to reveal that according to the sanitary-chemical indices 52,4 % of all the regions in which the portion of the non-standard samples was found to be in the range of 95% confidence interval (CI) [6,5-9,0] % of the average level have formed the more numerous (1st) cluster. The second cluster consisted of the Zaigrayevskiy and Bichurskiy regions in which the statistically significant increase in the prevalence of the non-standard samples was revealed. In 5 regions and the capital of Republic (Ulan-Ude) in the third cluster the most significant percent of the samples which don't accord to the hygienic claims (CI [15,0-29,0] %) is continuously registered. The fourth cluster consisted of three regions situated in the northern part of Buryat Republic in which the non-standard samples are extremely rare revealed (CI [0,5-2,2] %). The samples portion which don't accord to the hygienic normative in the microbiological indices was found to increase by 1,4-fold compared to the 2009-2011 years and amounted 3,8 % in 2012 year. The analysis based on the territorial factor has shown that the high levels of the non-standard samples of the food products in the microbiological indices were found to be in the regions of the first cluster: Eravninskiy, Mukhorshibirskiy, Kabanskiy and Ulan-Ude (CI [5,1-7,8] %), the last revealed level is the regions of the third cluster: Zakamenskiy, Bichurskiy, Barguzinskiy, Kurumkanskiy, Okinskiy, Bauntovskiy (CI [0,10-0,24] %). The control of microbiological safety allowed to reveal the most significant, in epidemic relation, food products: milk, meat, fish products, the products of children's food as well as the poultry products