91 research outputs found
ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π²ΠΈΠ΄ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΡΠΎΠ΄Π° Bifidobacterium ΠΊΠΈΡΠ΅ΡΠ½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠ΅ΡΠΎΠ΄Π° MALDI-TOF ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ
Background: The members of genus Bifidobacterium represent a significant part of intestinal microbiota in adults and predominate in infants. Species repertoire of the intestinal bifidobacteria is known to be subjected to major changes with age; however, many details of this process are still to be elucidated.Objective: Our aim was to study the diversity of intestinal bifidobacteria and changes of their qualitative and quantitative composition characteristics during the process of growing up using MALDI-TOF mass-spectrometric analysis of pure bacterial cultures.Methods: A cross-sectional study of bifidobacteria in the intestinal microbiota was performed in 93 healthy people of the ages from 1 month to 57 years. Strains were identified using Microflex LT MALDI-TOF MS, the confirmation was performed by 16S rRNA gene fragment sequencing.Results: 93% of isolated bifidobacterial strains were successfully identified using MALDI-TOF mass-spectrometry. At least two of the strains from each species were additionally identified by 16S rRNA gene fragment sequencing, in all of the cases the results were the same. It was shown that the total concentration of bifidobacteria decreases with age (p 0.001) as well as the frequency of isolation of Bifidobacterium bifidum (p =0.020) and Bifidobacterium breve (p 0.001), and the frequency of isolation of Bifidobacterium adolescentis, increases (p 0.001), representing the continuous process of transformation of microbiota.Conclusion: The method of MALDI-TOF mass spectrometry demonstrated the ability to perform rapid and reliable identification of bifidobacteria that allowed the study of changes in the quantitative and qualitative characteristics of human microbiota in the process of growing up.ΠΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»ΠΈ ΡΠΎΠ΄Π° Bifidobacterium ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΡ ΡΠ°ΡΡΡ ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΡ ΠΊΠΈΡΠ΅ΡΠ½ΠΈΠΊΠ° Π²Π·ΡΠΎΡΠ»ΡΡ
Π»ΡΠ΄Π΅ΠΉ ΠΈ ΡΠΈΡΠ»Π΅Π½Π½ΠΎ Π΄ΠΎΠΌΠΈΠ½ΠΈΡΡΡΡ Π² ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΠ΅ ΠΌΠ»Π°Π΄Π΅Π½ΡΠ΅Π². ΠΠ·Π²Π΅ΡΡΠ½ΠΎ, ΡΡΠΎ Π²ΠΈΠ΄ΠΎΠ²ΠΎΠΉ ΡΠΎΡΡΠ°Π² ΠΊΠΈΡΠ΅ΡΠ½ΡΡ
Π±ΠΈΡΠΈΠ΄ΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π°Π΅ΡΡΡ ΡΠΈΠ»ΡΠ½ΡΠΌ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡΠΌ Ρ Π²ΠΎΠ·ΡΠ°ΡΡΠΎΠΌ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΌΠ½ΠΎΠ³ΠΈΠ΅ Π΄Π΅ΡΠ°Π»ΠΈ ΡΡΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΎΡΡΠ°ΡΡΡΡ Π½Π΅ΡΡΠ½ΡΠΌΠΈ.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ: ΠΈΠ·ΡΡΠΈΡΡ Π²ΠΈΠ΄ΠΎΠ²ΠΎΠ΅ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ Π±ΠΈΡΠΈΠ΄ΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΊΠΈΡΠ΅ΡΠ½ΠΈΠΊΠ° ΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΈΡ
ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ Π²Π·ΡΠΎΡΠ»Π΅Π½ΠΈΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΏΡΠΈ ΠΏΠΎΠΌΠΎΡΠΈ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ MALDI-TOF ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
ΠΏΡΠΎΡΠΈΠ»Π΅ΠΉ ΡΠΈΡΡΡΡ
ΠΊΡΠ»ΡΡΡΡ.ΠΠ΅ΡΠΎΠ΄Ρ: ΠΊΡΠΎΡΡ-ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΡ Π±ΠΈΡΠΈΠ΄ΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠΉ Π² ΡΠΎΡΡΠ°Π²Π΅ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΡ ΠΊΠΈΡΠ΅ΡΠ½ΠΈΠΊΠ° ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ Ρ 93 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 1 ΠΌΠ΅Ρ Π΄ΠΎ 57 Π»Π΅Ρ. ΠΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ Π²ΡΠ΄Π΅Π»Π΅- Π½ΠΈΠ΅ ΡΠΈΡΡΡΡ
ΠΊΡΠ»ΡΡΡΡ ΠΈ ΠΈΡ
ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π½Π° ΠΏΡΠΈΠ±ΠΎΡΠ΅ Microflex LT MALDI-TOF MS (Bruker Daltonics, ΠΠ΅ΡΠΌΠ°Π½ΠΈΡ), ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½ΠΈΠ΅ ΡΠ΅Π°Π»ΠΈΠ·ΠΎΠ²ΡΠ²Π°Π»ΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ° Π³Π΅Π½Π° 16S ΡΠ ΠΠ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ MALDI-TOF ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ Π±ΡΠ»ΠΎ ΡΡΠΏΠ΅ΡΠ½ΠΎ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½ΠΎ 93% Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΡΡ
ΡΡΠ°ΠΌΠΌΠΎΠ² Π±ΠΈΡΠΈΠ΄ΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠΉ. ΠΠΈΠ½ΠΈΠΌΡΠΌ ΠΏΠΎ 2 ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Ρ ΠΎΡ ΠΊΠ°ΠΆΠ΄ΠΎΠ³ΠΎ ΠΈΠ· Π²ΠΈΠ΄ΠΎΠ² Π±ΡΠ»ΠΈ Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ° Π³Π΅Π½Π° 16SΡΠ ΠΠ; Π²ΠΎ Π²ΡΠ΅Ρ
ΡΠ»ΡΡΠ°ΡΡ
ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΡΠΎΠ²ΠΏΠ°Π»ΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Ρ Π²ΠΎΠ·ΡΠ°ΡΡΠΎΠΌ ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΡ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΠΎΠ±ΡΠ΅ΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π±ΠΈΡΠΈΠ΄ΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠΉ (p 0,001), ΡΠΌΠ΅Π½ΡΡΠ°Π΅ΡΡΡ Π²ΡΡΡΠ΅ΡΠ°Π΅ΠΌΠΎΡΡΡ Π²ΠΈΠ΄ΠΎΠ² Bifidobacterium bifidum (p =0,020) ΠΈ Bifidobacterium breve (p 0,001), Π° Π²ΡΡΡΠ΅ΡΠ°Π΅ΠΌΠΎΡΡΡ Π²ΠΈΠ΄Π° Bifidobacterium adolescentis ΡΠ²Π΅Π»ΠΈΡΠΈΠ²Π°Π΅ΡΡΡ (p 0,001), ΠΎΡΡΠ°ΠΆΠ°Ρ ΠΏΠΎΡΡΠ΅ΠΏΠ΅Π½Π½ΡΠ΅ ΠΏΡΠΎΡΠ΅ΡΡΡ ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΠΈ ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΡ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅: ΠΌΠ΅ΡΠΎΠ΄ MALDI-TOF ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ ΠΏΠΎΠΊΠ°Π·Π°Π» Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ Π±ΡΡΡΡΠΎΠΉ ΠΈ Π½Π°Π΄Π΅ΠΆΠ½ΠΎΠΉ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π±ΠΈΡΠΈΠ΄ΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠΉ, ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ²ΡΠ΅ΠΉ ΠΏΡΠΎΠ²Π΅ΡΡΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΈ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ Π²Π·ΡΠΎΡΠ»Π΅Π½ΠΈ
Modulation control and spectral shaping of optical fiber supercontinuum generation in the picosecond regime
Numerical simulations are used to study how fiber supercontinuum generation
seeded by picosecond pulses can be actively controlled through the use of input
pulse modulation. By carrying out multiple simulations in the presence of
noise, we show how tailored supercontinuum Spectra with increased bandwidth and
improved stability can be generated using an input envelope modulation of
appropriate frequency and depth. The results are discussed in terms of the
non-linear propagation dynamics and pump depletion.Comment: Aspects of this work were presented in Paper ThJ2 at OECC/ACOFT 2008,
Sydney Australia 7-10 July (2008). Journal paper submitted for publication 30
July 200
The semileptonic decays of the B_c meson
We study the semileptonic transitions B_c to \eta_c, J/\psi, D, D^*, B, B^*,
B_s, B_s^* in the framework of a relativistic constituent quark model. We use
experimental data on leptonic J/\psi decay, lattice and QCD sum rule results on
leptonic B_c decay, and on radiative \eta_c transitions to adjust the quark
model parameters. We compute all form factors of the above semileptonic
B_c-transitions and give predictions for various semileptonic B_c decay modes
including their \tau-modes when they are kinematically accessible. The
implications of heavy quark symmetry for the semileptonic decays are discussed
and are shown to be manifest in our explicit relativistic quark model
calculation. A comparison of our results with the results of other calculations
is performed.Comment: 31 pages Latex (uses epsf, revtex). Section II expanded, typos
corrected. This version will appear in Phys. Rev.
Crossovers in Unitary Fermi Systems
Universality and crossover is described for attractive and repulsive
interactions where, respectively, the BCS-BEC crossover takes place and a
ferromagnetic phase transition is claimed. Crossovers are also described for
optical lattices and multicomponent systems. The crossovers, universal
parameters and phase transitions are described within the Leggett and NSR
models and calculated in detail within the Jastrow-Slater approximation. The
physics of ultracold Fermi atoms is applied to neutron, nuclear and quark
matter, nuclei and electrons in solids whenever possible. Specifically, the
differences between optical lattices and cuprates is discussed w.r.t.
antiferromagnetic, d-wave superfluid phases and phase separation.Comment: 50 pages, 15 figures. Contribution to Lecture Notes in Physics
"BCS-BEC crossover and the Unitary Fermi Gas" edited by W. Zwerge
Origins of the Ambient Solar Wind: Implications for Space Weather
The Sun's outer atmosphere is heated to temperatures of millions of degrees,
and solar plasma flows out into interplanetary space at supersonic speeds. This
paper reviews our current understanding of these interrelated problems: coronal
heating and the acceleration of the ambient solar wind. We also discuss where
the community stands in its ability to forecast how variations in the solar
wind (i.e., fast and slow wind streams) impact the Earth. Although the last few
decades have seen significant progress in observations and modeling, we still
do not have a complete understanding of the relevant physical processes, nor do
we have a quantitatively precise census of which coronal structures contribute
to specific types of solar wind. Fast streams are known to be connected to the
central regions of large coronal holes. Slow streams, however, appear to come
from a wide range of sources, including streamers, pseudostreamers, coronal
loops, active regions, and coronal hole boundaries. Complicating our
understanding even more is the fact that processes such as turbulence,
stream-stream interactions, and Coulomb collisions can make it difficult to
unambiguously map a parcel measured at 1 AU back down to its coronal source. We
also review recent progress -- in theoretical modeling, observational data
analysis, and forecasting techniques that sit at the interface between data and
theory -- that gives us hope that the above problems are indeed solvable.Comment: Accepted for publication in Space Science Reviews. Special issue
connected with a 2016 ISSI workshop on "The Scientific Foundations of Space
Weather." 44 pages, 9 figure
Single Spin Asymmetry in Polarized Proton-Proton Elastic Scattering at GeV
We report a high precision measurement of the transverse single spin
asymmetry at the center of mass energy GeV in elastic
proton-proton scattering by the STAR experiment at RHIC. The was measured
in the four-momentum transfer squared range \GeVcSq, the region of a significant interference between the
electromagnetic and hadronic scattering amplitudes. The measured values of
and its -dependence are consistent with a vanishing hadronic spin-flip
amplitude, thus providing strong constraints on the ratio of the single
spin-flip to the non-flip amplitudes. Since the hadronic amplitude is dominated
by the Pomeron amplitude at this , we conclude that this measurement
addresses the question about the presence of a hadronic spin flip due to the
Pomeron exchange in polarized proton-proton elastic scattering.Comment: 12 pages, 6 figure
Effect of event selection on jetlike correlation measurement in d+Au collisions at sNN=200Β GeV
AbstractDihadron correlations are analyzed in sNN=200Β GeV d+Au collisions classified by forward charged particle multiplicity and zero-degree neutral energy in the Au-beam direction. It is found that the jetlike correlated yield increases with the event multiplicity. After taking into account this dependence, the non-jet contribution on the away side is minimal, leaving little room for a back-to-back ridge in these collisions
J/Ο polarization in p+p collisions at s=200 GeV in STAR
AbstractWe report on a polarization measurement of inclusive J/Ο mesons in the di-electron decay channel at mid-rapidity at 2<pT<6 GeV/c in p+p collisions at s=200 GeV. Data were taken with the STAR detector at RHIC. The J/Ο polarization measurement should help to distinguish between different models of the J/Ο production mechanism since they predict different pT dependences of the J/Ο polarization. In this analysis, J/Ο polarization is studied in the helicity frame. The polarization parameter λθ measured at RHIC becomes smaller towards high pT, indicating more longitudinal J/Ο polarization as pT increases. The result is compared with predictions of presently available models
Beam-energy Dependence Of Charge Balance Functions From Au + Au Collisions At Energies Available At The Bnl Relativistic Heavy Ion Collider
Conselho Nacional de Desenvolvimento CientΓfico e TecnolΓ³gico (CNPq)Balance functions have been measured in terms of relative pseudorapidity (ΞΞ·) for charged particle pairs at the BNL Relativistic Heavy Ion Collider from Au + Au collisions at sNN=7.7GeV to 200 GeV using the STAR detector. These results are compared with balance functions measured at the CERN Large Hadron Collider from Pb + Pb collisions at sNN=2.76TeV by the ALICE Collaboration. The width of the balance function decreases as the collisions become more central and as the beam energy is increased. In contrast, the widths of the balance functions calculated using shuffled events show little dependence on centrality or beam energy and are larger than the observed widths. Balance function widths calculated using events generated by UrQMD are wider than the measured widths in central collisions and show little centrality dependence. The measured widths of the balance functions in central collisions are consistent with the delayed hadronization of a deconfined quark gluon plasma (QGP). The narrowing of the balance function in central collisions at sNN=7.7 GeV implies that a QGP is still being created at this relatively low energy. Β© 2016 American Physical Society.942CNPq, Conselho Nacional de Desenvolvimento CientΓfico e TecnolΓ³gicoMinistry of Education and Science of the Russian FederationMOE, Ministry of Education of the People's Republic of ChinaMOST, Ministry of Science and Technology of the People's Republic of ChinaNRF-2012004024, National Research FoundationNSF, National Stroke FoundationConselho Nacional de Desenvolvimento CientΓfico e TecnolΓ³gico (CNPq
J/Ο Production At Low Pt In Au+au And Cu+cu Collisions At Snn =200 Gev With The Star Detector
The J/Ο pT spectrum and nuclear modification factor (RAA) are reported for pT<5GeV/c and |y|<1 from 0% to 60% central Au+Au and Cu+Cu collisions at sNN=200GeV at STAR. A significant suppression of pT-integrated J/Ο production is observed in central Au+Au events. The Cu+Cu data are consistent with no suppression, although the precision is limited by the available statistics. RAA in Au+Au collisions exhibits a strong suppression at low transverse momentum and gradually increases with pT. The data are compared to high-pT STAR results and previously published BNL Relativistic Heavy Ion Collider results. Comparing with model calculations, it is found that the invariant yields at low pT are significantly above hydrodynamic flow predictions but are consistent with models that include color screening and regeneration. 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