251 research outputs found

    Early Detection of Mountain Pine Beetle Damage in Ponderosa Pine Forests of the Black Hills Using Hyperspectral and WorldView-2 Data

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    A leading cause for mortality in the pine forests of western North America, the mountain pine beetle, has impacted over 400,000 acres of ponderosa pine forest in the Black Hills of South Dakota since 1996. Methods aimed at earlier detection, prior to visual manifestation of a mountain pine beetle damage in the tree crown, have not been successful because of the overlap and variability of spectral response between the initial stages of attack (green-attacked) and non-attacked tree crowns. Needle-level reflectance spectra was measured from green-attack and non-attack ponderosa pine trees in early spring following an infestation and analyzed using a multi-statistical approach to determine which spectral features best discriminate green-attack needles. Green-attack reflectance was significantly higher than non-attack from 424-717 nm and 1151-2400 nm. Bands in the shortwave-infrared had increased measures of separation between classes compared to visible and near-infrared bands. Peaks in separation related to moisture absorption features, from 1451-1540 nm and 1973-2103 nm, and pigment absorption features from 462-520 nm and 663-689 nm, were consistently observed over multiple statistical analyses. While these features show promise for operational canopy-level detection, it is unknown if they can be scaled up due to large within-class variability and spectral overlap between classes. To examine the potential for canopy-level detection, in-situ training data was collected for green-attack and non-attack trees from known locations within the Black Hills at a similar time a WorldView-2 image was acquired of the study area. Along with eight WV-2 bands, all possible normalized two-band indices were calculated to examine the suitability of WV-2 data for detecting green-attack damage. The performance of three different classifiers, logistic regression, linear discriminant analysis, Random Forest, was evaluated. Normalized two-band indices using a combination of a near-infrared band and visible band increased separation compared to single WV-2 bands. Random Forest classifiers using the eight WV-2 bands as predictors yielded an independently validated accuracy of 70.6%. Compared to non-attack, green-attack class accuracies were lower, likely due to the high within-class variance and spectral overlap between classes observed. Even with these limitations, the methods presented offer improvements over existing green-attack detection methods

    Classic Period Dune Settlement in the Eastern Lower Papaloapan Basin, Southern Veracruz, Mexico

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    This dissertation is an archaeological investigation into the long-term settlement change of an ecologically distinct portion of the Eastern Lower Papaloapan Basin (ELPB) of southern Veracruz, Mexico, before, during, and after the fluorescence of the Tres Zapotes polity. This project examines the changing settlement history in an area of near-coastal paleodunes and estuarine lakes in the northern ELPB, addressing the question: “What processes account for variations in the distribution of occupation on the dune landscape through time?” I argue that the answer lies at the intersection of specific environmental, economic, and political factors in the ELPB over time

    A Visual Presentation of Man Prostituting Himself

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    This study involves the artistic presentation of the concept that: •’Man prostitutes himself and is trapped by his own prostitution. The chief problems of the thesis were the clarification of that concept and its subsequent interpretation in aesthetic form. Formulation of the basic idea demanded an examination of prostitution in its broader aspects. The use of man\u27s talents for base purposes was considered in the areas of public relations, business, corporation operation, art, education and religion. Then consideration was given to the assemblage as an aesthetic communicative device. This discussion involved the level of articulation available to the assemblage, and the emotive use of non-art objects in art work. A series of assemblages were projected to communicate the basic concept. The assemblages were first considered in view of their technical and aesthetic qualities. Their execution was then explored in detail. This exploration involved surface quality, material, the physical scale of the work, and the relation of the parts to the total work. The conclusions derived from this thesis were open-ended but indicated the potential of the assemblage as a method of aesthetic communication or concepts

    Virginia\u27s Proffer System and the Proffer Reform Act of 2016

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    Evidence-based practice in a social work context - the United States case

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    Sosiaalihuollon menetelmien arviointi (FinSoc) -projekt

    Pathway of histone mRNA degradation: oligouridylation followed by bidirectional decay

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    Histone mRNAs are rapidly degraded at the end of S phase or when DNA replication is inhibited. Histone mRNAs end in a conserved stemloop rather than a poly(A) tail. Degradation of histone mRNAs requires the stemloop sequence, which binds the stemloop binding protein (SLBP), active translation of the histone mRNA, and the location of the stemloop close to the termination codon. In this thesis I present evidence that the initial step in histone mRNA degradation is the addition of uridines to the 3' end of the histone mRNA, both after inhibition of DNA replication and at the end of S-phase. Lsm1 is required for histone mRNA degradation and is present in a complex containing SLBP on the 3' end of histone mRNA after inhibition of DNA replication. I cloned degradation intermediates that had been partially degraded from both the 5' and the 3' end. RNA interference (RNAi) experiments demonstrate that both the exosome (3'-5') and 5' to 3' decay pathway components are functionally required for degradation. cRT-PCR experiments corroborate the findings from the functional RNAi experiments by providing direct evidence that individual histone mRNAs are degraded simultaneously 5' to 3' and 3' to 5' when DNA synthesis is inhibited. Finally, I present evidence that SLBP protein expression is required for proper regulation of histone mRNA degradation when DNA synthesis is inhibited, but that the underlying mechanism is due to nuclear retention of properly processed histone mRNA. The latter suggests that one of the critical functions of SLBP in human cells is the proper export of histone mRNA to the cytoplasm
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