Measuring Local Genetic Variability in Populations of Codling Moth (Lepidoptera: Tortricidae) Across an Unmanaged and Commercial Orchard Interface

The genetic structure of adult codling moth, Cydia pomonella (L.), populations was characterized both inside a managed apple, Malus domestica Borkdhausen, orchard and in surrounding unmanaged hosts and nonhost trees in central Chile during 2006–2007. Adult males were collected using an array of sex pheromone-baited traps. Five microsatellite genetic markers were used to study the population genetic structure across both spatial (1–100 ha) and temporal (generations within a season) gradients. Analysis of molecular variance (AMOVA) found a significant, but weak, association in both the spatial and temporal genetic structures. Discriminant analysis also found significant differentiation between the first and second generation for traps located either inside or outside the managed orchard. The Bayesian assignment test detected three genetic clusters during each of the two generations, which corresponded to different areas within the unmanaged and managed apple orchard interface. The lack of a strong spatial structure at a local scale was hypothesized to be because of active adult movement between the managed and unmanaged hosts and the asymmetry in the insecticide selection pressure inside and outside the managed habitats. These data highlight the importance of developing area-wide management programs that incorporate management tactics effective at the landscape level for successful codling moth control.This is the publisher’s final pdf. The published article is copyrighted by the Entomological Society of America and can be found at: http://www.bioone.org/loi/enveKeywords: Cydia pomonella, TESS, Bayesian assignment, Genetic structure, Microsatellit

¿Es Alstroemeria pulchra Sims var. maxima Phil. (Alstroemeriaceae) un miembro del complejo A. magnifica Herbert?: Estudio colorimétrico de los tépalos externos e internos de la flor*

Alstroemeria pulchra var. maxima is an endemic Chilean taxon described by R.A. Philippi in 1864, whose taxonomic position iscontroversial; even though Bayer in 1987 transferred this taxon to A. magnifica with the rank of subspecies, Muñoz & Moreira in2003 return to the original concept proposed by Philippi. In order to provide a new character to decide if A. pulchra var. maxima is amember of the A. magnifica complex or must remain as a variety of A. pulchra as was originally described, the color of outer, upperinner and lower inner tepals of the flowers of eight populations of Alstroemeria L. belonging to these two endemic Chilean specieswere analyzed with a spectrophotometer colorimeter Hunterlab Colorquest: five populations of A. magnifica Herbert and threepopulations of A. pulchra Sims. CIELab coordinates allow to separate A. magnifica from A. pulchra on the basis of the color of theouter and lower inner tepals but not by the color of the upper inner tepals. We conclude that the color of the flower is a new characterto confirm that var. maxima belong to A. pulchra, not to the A. magnifica complex.Alstroemeria pulchra var. maxima es un taxón endémico de Chile descrito por R.A. Philippi en 1864, cuya posición sistemática escontroversial; aunque Bayer en 1987 transfiere este taxón a A. magnifica con el rango de subespecie, Muñoz & Moreira en 2003retoman el concepto original de Philippi. Con el objetivo de proporcionar nuevos caracteres para decidir si A. pulchra var. maximaes un miembro del complejo A. magnifica o debe permanecer como una variedad de A. pulchra como fue originalmente descrita, seanalizó el color de los tépalos externos, internos superiores e interno inferior, utilizando un espectrofotómetro colorímetro HunterlabColorquest. Se trabajó con flores de ocho poblaciones de Alstroemeria L. pertenecientes a estas dos especies endémicas de Chile:cinco poblaciones de A. magnifica Herbert y tres poblaciones de A. pulchra Sims. Las mediciones en el espacio CIELab permitendistinguir A. magnifica de A. pulchra sobre la base del color de los tépalos externos y del tépalo interno inferior, pero no por el colorde los tépalos internos superiores. Se concluye que el color de la flor es un nuevo carácter que confirma que la var. maxima pertenecea A. pulchra y no al complejo A. magnifica

Metal-THINGS: On the metallicity and ionization of ULX sources in NGC 925

We present an analysis of the optical properties of three Ultra Luminous X-ray (ULX) sources identified in NGC 925. We use Integral field unit data from the George Mitchel spectrograph in the context of the Metal-THINGS survey. The optical properties for ULX-1 and ULX-3 are presented, while the spaxel associated with ULX-2 had a low S/N, which prevented its analysis. We also report the kinematics and dimensions of the optical nebula associated with each ULX using ancillary data from the PUMA Fabry-Perot spectrograph. A BPT analysis demonstrates that most spaxels in NGC 925 are dominated by star-forming regions, including those associated with ULX-1 and ULX-3. Using the resolved gas-phase metallicities, a negative metallicity gradient is found, consistent with previous results for spiral galaxies, while the ionization parameter tends to increase radially throughout the galaxy. Interestingly, ULX-1 shows a very low gas metallicity for its galactocentric distance, identified by two independent methods, while exhibiting a typical ionization. We find that such low gas metallicity is best explained in the context of the high-mass X-ray binary population, where the low-metallicity environment favours active Roche lobe overflows that can drive much higher accretion rates. An alternative scenario invoking accretion of a low-mass galaxy is not supported by the data in this region. Finally, ULX-3 shows both a high metallicity and ionization parameter, which is consistent with the progenitor being a highly-accreting neutron star within an evolved stellar population region.Comment: Accepted by Ap

BRAIN & SPINAL CORD DAMAGE & REHABILITATION

Stroke and traumatic injury in brain or spinal cord are often life-threating conditions and major causes of death or permanent disability with high impact in the health care system. There are several stages of intervention to improve the neurological outcome. Acutely, fast interventions aiming to reestablish cerebral blood flow in ischemic stroke, to stop bleeding after brain hemorrhage, and to reduce edema after contusions are amongst mandatory actions. Current studies aim to develop accompanying strategies for brain cell protection based on enhancing endogenous protective mechanism, blocking cell death pathways, or through immunomodulation. After the acute phase, interventions are intended to promote recovery of function using rehabilitation with state-of-the-art technologies enabled by robotics. Other advanced strategies include cell, gene, and immune therapies, and brain function modulation with the aid of smart nanotechnologies. There is great expectation in the fast evolving novel approaches for improvement of neurological deficits in these unpredictable and devastating conditionPeer reviewe

Catálogo de las plantas vasculares de Chile

A catalog of vascular plants growing in Chile is presented. It is organized by divisions, Pteridophyta (Lycopodiopsida and Polypodiopsida), Pinophyta (Gnetopsida and Pinopsida) and Magnoliophyta (Liliopsida and Magnoliopsida), and within each group, the taxonomic hierarchies (Family, Genus, Species and infraspecific taxa) are arranged alphabetically. In accordance with this catalogue, the flora of vascular plants of Chile comprise 186 families, 1121 genera and 5471 species, 4655 species are native, 2145 of these are endemic to Chile and 816 species are introduced.Se presenta un catálogo de las plantas vasculares que crecen en Chile. Está organizado por divisiones, Pteridophyta (Lycopodiopsida y Polypodiopsida), Pinophyta (Gnetopsida y Pinopsida) y Magnoliophyta (Liliopsida y Magnoliopsida), y dentro de cada grupo, las jerarquías taxonómicas (Familia, Género, Especies y taxones infraespecíficos) están ordenados alfabéticamente. Se incluye además un índice alfabético de géneros con indicación de la familia y grupo a que pertenecen. De acuerdo a este catálogo la flora de las plantas vasculares que crecen en Chile, comprende 186 familias, 1121 géneros y especies, de éstas, 4655 corresponden a especies nativas, de las cuales 2145 son endémicas de Chile y 816 las especies introducidas

The Relationship between Depressive Symptoms, Quality of Life and miRNAs 8 Years after Bariatric Surgery

Altres ajuts: Universidad de Málaga, UMA20-FEDERJA-144; Junta de Andalucia, PI-0194-2017, C-0028-2018, RC-005-2020, DOC_00288 and DOC_01095Background: There are conflicting results on whether weight loss after bariatric surgery (BS) might be associated with quality of life (QoL)/depressive symptomatology. We aim to determine whether BS outcomes are associated with QoL/depressive symptomatology in studied patients at the 8-year follow-up after BS, as well as their relationship with different serum proteins and miRNAs. Methods: A total of 53 patients with class III obesity who underwent BS, and then classified into "good responders" and "non-responders" depending on the percentage of excess weight lost (%EWL) 8 years after BS (%EWL ≥ 50% and %EWL < 50%, respectively), were included. Basal serum miRNAs and different proteins were analysed, and patients completed tests to evaluate QoL/depressive symptomatology at 8 years after BS. Results: The good responders group showed higher scores on SF-36 scales of physical functioning, role functioning-physical, role functioning-emotional, body pain and global general health compared with the non-responders. The expression of hsa-miR-101-3p, hsa-miR-15a-5p, hsa-miR-29c-3p, hsa-miR-144-3p and hsa-miR-19b-3p were lower in non-responders. Hsa-miR-19b-3p was the variable associated with the response to BS in a logistic regression model. Conclusions: The mental health of patients after BS is limited by the success of the intervention. In addition, the expression of basal serum miRNAs related to depression/anxiety could predict the success of BS

The intrinsically disordered, epigenetic factor RYBP binds to the citrullinating enzyme PADI4 in cancer cells

14 p.-6 fig.-1 tab.RYBP (Ring1 and YY 1 binding protein) is a multifunctional, intrinsically disordered protein (IDP), best described as a transcriptional regulator. It exhibits a ubiquitin-binding functionality, binds to other transcription factors, and has a key role during embryonic development. RYBP, which folds upon binding to DNA, has a Zn-finger domain at its N-terminal region. By contrast, PADI4 is a well-folded protein and it is one the human isoforms of a family of enzymes implicated in the conversion of arginine to citrulline. As both proteins intervene in signaling pathways related to cancer development and are found in the same localizations within the cell, we hypothesized they may interact. We observed their association in the nucleus and cytosol in several cancer cell lines, by using immunofluorescence (IF) and proximity ligation assays (PLAs). Binding also occurred in vitro, as measured by isothermal titration calorimetry (ITC) and fluorescence, with a low micromolar affinity (~1 μM). AlphaFold2-multimer (AF2) results indicate that PADI4's catalytic domain interacts with the Arg53 of RYBP docking into its active site. As RYBP sensitizes cells to PARP (Poly (ADP-ribose) polymerase) inhibitors, we applied them in combination with an enzymatic inhibitor of PADI4 observing a change in cell proliferation, and the hampering of the interaction of both proteins. This study unveils for the first time the possible citrullination of an IDP, and suggests that this new interaction, whether it involves or not citrullination of RYBP, might have implications in cancer development and progression.This research was funded by Ministry of Science and Innovation MCIN/AEI/10.13039/501100011033/ and “ERDF A way of Making Europe” [PID2021-127296OB-I00 to AVC; and PDC2022-133952-I00 to EF]; by Instituto de Salud Carlos III co-funded by European Social Fund “Investing in your future” [CP19/00095 to CdJ] [PI22/00824 to MS and CdJ] [PI18/00394 to OA]; by Diputación General de Aragón [“Protein targets and Bioactive Compounds group” E45-20R to AVC, and “Digestive Pathology Group” B25-20R to OA], and by Consellería de Innovación, Universidades, Ciencia y Sociedad Digital (Generalitat Valenciana) [CAICO 2021/0135 to CdJ and JLN].Peer reviewe