Three species of Colletotrichum are associated with anthracnose of pomegranate in Brazil

Pomegranate is a fruit rich in vitamins and secondary metabolites used in traditional medicine and industry. However, production losses have been associated the anthracnose, disease caused by Colletotrichum species. This is an important disease of the pomegranate, as it affects the yield and the quality of the fruits. The present study aimed to investigate which species of Colletotrichum are associated with anthracnose disease in pomegranate in the Northeast region of Brazil, using multi-locus phylogenetic analysis and morpho-cultural characteristics. The total DNA extracted was amplified with GAPDH, TUB2, CAL, ACT genes and the ITS-rDNA region. The sequences obtained were used for the construction of phylogenetic trees of Bayesian inference. The mycelial growth rate, size and shape of the conidia and appressories were evaluated for the morpho-cultural characterization of the species. Six isolates were analysis and three species belonging to the C. gloeosporioides complex were identified in this study. This is the first report of C. theobromicola (2) in pomegranate fruit in Brazil and C. siamense (2) and C. fructicola (2) in the world.Pomegranate is a fruit rich in vitamins and secondary metabolites used in traditional medicine and industry. However, production losses have been associated the anthracnose, disease caused by Colletotrichum species. This is an important disease of the pomegranate, as it affects the yield and the quality of the fruits. The present study aimed to investigate which species of Colletotrichum are associated with anthracnose disease in pomegranate in the Northeast region of Brazil, using multi-locus phylogenetic analysis and morpho-cultural characteristics. The total DNA extracted was amplified with GAPDH, TUB2, CAL, ACT genes and the ITS-rDNA region. The sequences obtained were used for the construction of phylogenetic trees of Bayesian inference. The mycelial growth rate, size and shape of the conidia and appressories were evaluated for the morpho-cultural characterization of the species. Six isolates were analysis and three species belonging to the C. gloeosporioides complex were identified in this study. This is the first report of C. theobromicola (2) in pomegranate fruit in Brazil and C. siamense (2) and C. fructicola (2) in the world

Anais do V Encontro Brasileiro de Educomunicação: Educação midiática e políticas públicas

A presente coletânea, que chega ao público através de um suporte digital, tem como objetivo disponibilizar os papers, bem como os relatos de experiências educomunicativas apresentados durante o V ENCONTRO BRASILEIRO DE EDUCOMUNICAÇÃO, que teve como tema central: “Educação Midiática e Políticas Públicas”. O evento foi realizado em São Paulo, entre 19 e 21 de setembro de 2013, a partir de uma parceria entre o NCE/USP - Núcleo de Comunicação e Educação da USP, a Licenciatura em Educomunicação da ECA/USP, a ABPEducom – Associação Brasileira de Pesquisadores e Profissionais da Educomunicação e a FAPCOM – Faculdade Paulus de Tecnologia e Comunicação, que ofereceu seu campus, na Vila Mariana, para os atos do evento. Os presentes anais disponibilizam o texto de abertura, de autoria do coordenador geral do evento, denominado “Educação midiática e políticas públicas: vertentes históricas da emergência da Educomunicação na América Latina”. Na sequência, apresentam 61 papers sobre aspectos específicos da temática geral, resultantes de pesquisas na área, seguidos de 27 relatos de práticas educomunicativas, em nível nacional

Armazenamento e germina\ue7\ue3o de sementes de Tabebuia aurea (manso) Benth. & Hook. f. ex. S. Moore

As sementes foram acondicionadas em sacos de papel, de algodão e plástico de natureza permeável, em ambiente frio e seco (15ºC e 40% de UR) por quatro meses. Inicialmente, testou-se a influência da presença e ausência contínua de luz branca e fotoperíodo de 12 h, a fim de determinar o fotoblastismo das sementes, na temperatura de 25ºC. Verificou-se a influência das temperaturas constantes de 10, 15, 20, 25, 30, 35 e 40ºC na germinação e determinou-se as temperaturas cardeais (mínima, ótima e máxima). Testou-se a viabilidade das sementes aos 60, 90 e 120 dias de armazenamento, empregando-se teste de germinação, e a velocidade de embebição aos 30, 60 e 90 dias, utilizando-se amostras provenientes de cada tipo de embalagem. As embalagens utilizadas no armazenamento não afetaram significativamente as variáveis estudadas. A espécie em estudo pode ser classificada como fotoblástica neutra à 25ºC, com percentagem média de germinação alta, variando de 88 a 98%. A temperatura ótima de germinação foi de 35ºC, a mínima de 20ºC e a máxima de 40ºC, na ausência de luz. As embalagens utilizadas no armazenamento mantiveram a viabilidade das sementes por até 120 dias, com altos percentuais de germinação, variando de 88 a 97%. As sementes apresentaram velocidade de embebição rápida atingindo 100% a partir das quatro horas de embebição

Improving biophysical models to link terrestrial, riverine and coastal interfaces accounting for biodiversity and ES

International audienc

Direct antiviral therapy for treatment of hepatitis C: A real-world study from Brazil

Introduction and objectives: Direct antiviral agents (DAAs) including sofosbuvir (SOF), daclatasvir (DCV), simeprevir (SIM) and ombitasvir, paritaprevir and dasabuvir were introduced 2015 in Brazil for treatment of hepatitis C virus (HCV) infection. The aims of this study were to assess effectiveness and safety of HCV treatment with DAA in real-life world in a highly admixed population from Brazil. Materials and methods: All Brazilian reference centers for HCV treatment were invited to take part in a web-based registry, prospectively conducted by the Brazilian Society of Hepatology, to assess outcomes of HCV treatment in Brazil with DAAs. Data to be collected included demographics, disease severity and comorbidities, genotype (GT), viral load, DAA regimens, treatment side effects and sustained virological response (SVR). Results: 3939 patients (60% males, mean age 58 ± 10 years) throughout the country were evaluated. Most had advanced fibrosis or cirrhosis, GT1 and were treated with SOF/DCV or SOF/SIM. Overall SVR rates were higher than 95%. Subjects with decompensated cirrhosis, GT2 and GT3 have lower SVR rates of 85%, 90% and 91%, respectively. Cirrhosis and decompensated cirrhosis in GT1 and male sex and decompensated cirrhosis in GT3 were significantly associated with no SVR. Adverse events (AD) and serious AD occurred in 18% and 5% of those subjects, respectively, but less than 1% of patients required treatment discontinuation. Conclusion: SOF-based DAA regimens are effective and safe in the heterogeneous highly admixed Brazilian population and could remain an option for HCV treatment at least in low-income countries