1,229 research outputs found
Patient-specific iPSCs carrying an SFTPC mutation reveal the intrinsic alveolar epithelial dysfunction at the inception of interstitial lung disease
Alveolar epithelial type 2 cell (AEC2) dysfunction is implicated in the pathogenesis of adult and pediatric interstitial lung disease (ILD), including idiopathic pulmonary fibrosis (IPF); however, identification of disease-initiating mechanisms has been impeded by inability to access primary AEC2s early on. Here, we present a human in vitro model permitting investigation of epithelial-intrinsic events culminating in AEC2 dysfunction, using patient-specific induced pluripotent stem cells (iPSCs) carrying an AEC2-exclusive disease-associated variant (SFTP
Extracapsular tumor spread and the risk of local, axillary and supraclavicular recurrence in node-positive, premenopausal patients with breast cancer
BACKGROUND: Extracapsular tumor spread (ECS) has been identified as a possible risk factor for breast cancer recurrence, but controversy exists regarding its role in decision making for regional radiotherapy. This study evaluates ECS as a predictor of local, axillary, and supraclavicular recurrence. PATIENTS AND METHODS: International Breast Cancer Study Group Trial VI accrued 1475 eligible pre- and perimenopausal women with node-positive breast cancer who were randomly assigned to receive three to nine courses of classical combination chemotherapy with cyclophosphamide, methotrexate, and fluorouracil. ECS status was determined retrospectively in 933 patients based on review of pathology reports. Cumulative incidence and hazard ratios (HRs) were estimated using methods for competing risks analysis. Adjustment factors included treatment group and baseline patient and tumor characteristics. The median follow-up was 14 years. RESULTS: In univariable analysis, ECS was significantly associated with supraclavicular recurrence (HR = 1.96; 95% confidence interval 1.23-3.13; P = 0.005). HRs for local and axillary recurrence were 1.38 (P = 0.06) and 1.81 (P = 0.11), respectively. Following adjustment for number of lymph node metastases and other baseline prognostic factors, ECS was not significantly associated with any of the three recurrence types studied. CONCLUSIONS: Our results indicate that the decision for additional regional radiotherapy should not be based solely on the presence of ECS
Water structuring and collagen adsorption at hydrophilic and hydrophobic silicon surfaces
The adsorption of a collagen fragment on both a hydrophobic,
hydrogen-terminated and a hydrophilic, natively oxidised Si surface is
investigated using all-atom molecular dynamics. While favourable direct
protein-surface interactions via localised contact points characterise adhesion
to the hydrophilic surface, evenly spread surface/molecule contacts and
stabilisation of the helical structure occurs upon adsorption on the
hydrophobic surface. In the latter case, we find that adhesion is accompanied
by a mutual fit between the hydrophilic/hydrophobic pattern within the protein
and the layered water structure at the solid/liquid interface, which may
provide an additional driving force to the classic hydrophobic effect
Worldwide Disparities in Recovery of Cardiac Testing 1 Year Into COVID-19
BACKGROUND
The extent to which health care systems have adapted to the COVID-19 pandemic to provide necessary cardiac diagnostic services is unknown.
OBJECTIVES
The aim of this study was to determine the impact of the pandemic on cardiac testing practices, volumes and types of diagnostic services, and perceived psychological stress to health care providers worldwide.
METHODS
The International Atomic Energy Agency conducted a worldwide survey assessing alterations from baseline in cardiovascular diagnostic care at the pandemic's onset and 1 year later. Multivariable regression was used to determine factors associated with procedure volume recovery.
RESULTS
Surveys were submitted from 669 centers in 107 countries. Worldwide reduction in cardiac procedure volumes of 64% from March 2019 to April 2020 recovered by April 2021 in high- and upper middle-income countries (recovery rates of 108% and 99%) but remained depressed in lower middle- and low-income countries (46% and 30% recovery). Although stress testing was used 12% less frequently in 2021 than in 2019, coronary computed tomographic angiography was used 14% more, a trend also seen for other advanced cardiac imaging modalities (positron emission tomography and magnetic resonance; 22%-25% increases). Pandemic-related psychological stress was estimated to have affected nearly 40% of staff, impacting patient care at 78% of sites. In multivariable regression, only lower-income status and physicians' psychological stress were significant in predicting recovery of cardiac testing.
CONCLUSIONS
Cardiac diagnostic testing has yet to recover to prepandemic levels in lower-income countries. Worldwide, the decrease in standard stress testing is offset by greater use of advanced cardiac imaging modalities. Pandemic-related psychological stress among providers is widespread and associated with poor recovery of cardiac testing
The Star Formation History in The Far Outer Disc of M33
The outer regions of disc galaxies are becoming increasingly recognized as
key testing sites for models of disc assembly and evolution. Important issues
are the epoch at which the bulk of the stars in these regions formed and how
discs grow radially over time. To address these issues, we use Hubble Space
Telescope Advanced Camera for Surveys imaging to study the star formation
history (SFH) of two fields at 9.1 and 11.6 kpc along M33's northern major
axis. These fields lie at ~ 4 and 5 V-band disc scale-lengths and straddle the
break in M33's surface brightness profile. The colour-magnitude diagrams (CMDs)
reach the ancient main sequence turnoff with a signal-to-noise ratio of ~ 5.
From detailed modelling of the CMDs, we find that the majority of stars in both
fields combined formed at z < 1. The mean age in the inner field, S1, is ~ 3
+/- 1 Gyr and the mean metallicity is [M/H] ~ -0.5 +/- 0.2 dex. The star
formation history of S1 unambiguously reveals how the inside-out growth
previously measured for M33's inner disc out to ~ 6 kpc extends out to the disc
edge at ~ 9 kpc. In comparison, the outer field, S2, is older (mean age ~ 7 +/-
2 Gyr), more metal-poor (mean [M/H] ~ -0.8 +/- 0.3 dex), and contains ~ 30
times less stellar mass. These results provide the most compelling evidence yet
that M33's age gradient reverses at large radii near the disc break and that
this reversal is accompanied by a break in stellar mass surface density. We
discuss several possible interpretations of this behaviour including radial
stellar mixing, warping of the gaseous disc, a change in star formation
efficiency, and a transition to another structural component. These results
offer one of the most detailed views yet of the peripheral regions of any disc
galaxy and provide a much-needed observational constraint on the last major
epoch of star formation in the outer disc.Comment: 15 pages, 9 figures, accepted to MNRAS, hi-res version available at
http://www.roe.ac.uk/~mkb/astroph/m33hires.pd
Quantum phase transitions of light
Recently, condensed matter and atomic experiments have reached a length-scale
and temperature regime where new quantum collective phenomena emerge. Finding
such physics in systems of photons, however, is problematic, as photons
typically do not interact with each other and can be created or destroyed at
will. Here, we introduce a physical system of photons that exhibits strongly
correlated dynamics on a meso-scale. By adding photons to a two-dimensional
array of coupled optical cavities each containing a single two-level atom in
the photon-blockade regime, we form dressed states, or polaritons, that are
both long-lived and strongly interacting. Our zero temperature results predict
that this photonic system will undergo a characteristic Mott insulator
(excitations localised on each site) to superfluid (excitations delocalised
across the lattice) quantum phase transition. Each cavity's impressive photon
out-coupling potential may lead to actual devices based on these quantum
many-body effects, as well as observable, tunable quantum simulators. We
explicitly show that such phenomena may be observable in micro-machined diamond
containing nitrogen-vacancy colour centres and superconducting microwave
strip-line resonators.Comment: 11 pages, 5 figures (2 in colour
Body composition and body fat distribution are related to cardiac autonomic control in non-alcoholic fatty liver disease patients
BACKGROUND/OBJECTIVES: Heart rate recovery (HRR), a cardiac autonomic control marker, was shown to be related to body composition (BC), yet this was not tested in non-alcoholic fatty liver disease (NAFLD) patients. The aim of this study was to determine if, and to what extent, markers of BC and body fat (BF) distribution are related to cardiac autonomic control in NAFLD patients. SUBJECTS/METHODS: BC was assessed with dual-energy X-ray absorptiometry in 28 NAFLD patients (19 men, 51±13 years, and 9 women, 47±13 years). BF depots ratios were calculated to assess BF distribution. Subjects’ HRR was recorded 1 (HRR1) and 2 min (HRR2) immediately after a maximum graded exercise test.
RESULTS: BC and BF distribution were related to HRR; particularly weight, trunk BF and trunk BF-to-appendicular BF ratio showed a negative relation with HRR1 (r 1⁄4 0.613, r 1⁄4 0.597 and r 1⁄4 0.547, respectively, Po0.01) and HRR2 (r 1⁄4 0.484, r 1⁄4 0.446, Po0.05, and r 1⁄4 0.590, Po0.01, respectively). Age seems to be related to both HRR1 and HRR2 except when controlled for BF distribution. The preferred model in multiple regression should include trunk BF-to-appendicular BF ratio and BF to predict HRR1 (r2 1⁄4 0.549; Po0.05), and trunk BF-to-appendicular BF ratio alone to predict HRR2 (r2 1⁄4 0.430; Po0.001).
CONCLUSIONS: BC and BF distribution were related to HRR in NAFLD patients. Trunk BF-to-appendicular BF ratio was the best independent predictor of HRR and therefore may be best related to cardiovascular increased risk, and possibly act as a mediator in age-related cardiac autonomic control variation.info:eu-repo/semantics/publishedVersio
High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source
High-resolution microcomputed tomography with benchtop X-ray sources requires long scan times because of the heat load limitation on the anode. We present an alternative, high-brightness plasma-based X-ray source that does not suffer from this restriction. A demonstration of tomography of a centimeter-scale complex organism achieves equivalent quality to a commercial scanner. We will soon be able to record such scans in minutes, rather than the hours required by conventional X-ray tubes
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