Magnetic Properties of Dilute Alloys: Equations for Magnetization and its Structural Fluctuations

The dilute Heisenberg ferromagnet is studied taking into account fluctuations of magnetization caused by disorder. A self-consistent system of equations for magnetization and its mean quadratic fluctuations is derived within the configurationally averaged two-time temperature Green's function method. This system of equations is analised at low concentration of non-magnetic impurities. Mean relative quadratic fluctuations of magnetization are revealed to be proportional to the square of concentration of impurities.Comment: 16 pages, LaTe

Cloning and characterization of 5 '-upstream region of human phospholipase C-beta 2 gene

5 ' -upstream region of the phospholipase C-beta2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284 nucleotides upstream from the beginning of translation. The 5 ' -upstream region lacks both TATA motif and typical initiator sequence, but retains CC-rich segment. Two putative regulatory regions, a negative region (-636/-588) and a positive region (-98/-13) were identified in the upstream region of PLC-beta2 gene. We suggest that the transcription of PLC-beta2 may be regulated by binding of regulatory proteins to the negative and/or positive regulatory regions located in the upstream of the geneopen

Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans-splicing group I introns

<p>Abstract</p> <p>Background</p> <p>Dengue viruses (DENV) are one of the most important viral diseases in the world with approximately 100 million infections and 200,000 deaths each year. The current lack of an approved tetravalent vaccine and ineffective insecticide control measures warrant a search for alternatives to effectively combat DENV. The <it>trans</it>-splicing variant of the <it>Tetrahymena thermophila </it>group I intron catalytic RNA, or ribozyme, is a powerful tool for post-transcriptional RNA modification. The nature of the ribozyme and the predictability with which it can be directed makes it a powerful tool for modifying RNA in nearly any cell type without the need for genome-altering gene therapy techniques or dependence on native cofactors.</p> <p>Results</p> <p>Several anti-DENV Group I <it>trans</it>-splicing introns (αDENV-GrpIs) were designed and tested for their ability to target DENV-2 NGC genomes <it>in situ</it>. We have successfully targeted two different uracil bases on the positive sense genomic strand within the highly conserved 5'-3' cyclization sequence (CS) region common to all serotypes of DENV with our αDENV-GrpIs. Our ribozymes have demonstrated ability to specifically <it>trans</it>-splice a new RNA sequence downstream of the targeted site <it>in vitro </it>and in transfected insect cells as analyzed by firefly luciferase and RT-PCR assays. The effectiveness of these αDENV-GrpIs to target infecting DENV genomes is also validated in transfected or transformed Aedes mosquito cell lines upon infection with unattenuated DENV-2 NGC.</p> <p>Conclusions</p> <p>Analysis shows that our αDENV-GrpIs have the ability to effectively <it>trans</it>-splice the DENV genome <it>in situ</it>. Notably, these results show that the αDENV-GrpI 9v1, designed to be active against all forms of Dengue virus, effectively targeted the DENV-2 NGC genome in a sequence specific manner. These novel αDENV-GrpI introns provide a striking alternative to other RNA based approaches for the transgenic suppression of DENV in transformed mosquito cells and tissues.</p

On the complexity of the Saccharomyces bayanus taxon: hybridization and potential hybrid speciation

Although the genus Saccharomyces has been thoroughly studied, some species in the genus has not yet been accurately resolved; an example is S. bayanus, a taxon that includes genetically diverse lineages of pure and hybrid strains. This diversity makes the assignation and classification of strains belonging to this species unclear and controversial. They have been subdivided by some authors into two varieties (bayanus and uvarum), which have been raised to the species level by others. In this work, we evaluate the complexity of 46 different strains included in the S. bayanus taxon by means of PCR-RFLP analysis and by sequencing of 34 gene regions and one mitochondrial gene. Using the sequence data, and based on the S. bayanus var. bayanus reference strain NBRC 1948, a hypothetical pure S. bayanus was reconstructed for these genes that showed alleles with similarity values lower than 97% with the S. bayanus var. uvarum strain CBS 7001, and of 99¿100% with the non S. cerevisiae portion in S. pastorianus Weihenstephan 34/70 and with the new species S. eubayanus. Among the S. bayanus strains under study, different levels of homozygosity, hybridization and introgression were found; however, no pure S. bayanus var. bayanus strain was identified. These S. bayanus hybrids can be classified into two types: homozygous (type I) and heterozygous hybrids (type II), indicating that they have been originated by different hybridization processes. Therefore, a putative evolutionary scenario involving two different hybridization events between a S. bayanus var. uvarum and unknown European S. eubayanus-like strains can be postulated to explain the genomic diversity observed in our S. bayanus var. bayanus strains

STUDI EKSPERIMENTAL KARAKTERISTIK KUAT TEKAN DAN KARAKTERISTIK PEMBAKARAN BRIKET DAUN CENGKEH DAN JERAMI PADI

Penelitian ini mempelajari tentang karakteristik kuat tekan dan karakteristik pembakaran briket daun cengkeh dan jerami padi. Pembriketan dilakukan dengan menggunakan mesin pres hidrolik dengan tekanan pembriketan sebesar 450 kg/cm2, dengan bahan pengikat dan tanpa bahan pengikat. Bahan pengikat yang digunakan adalah lem kanji dengan kadar 5 %. Briket berbentuk silinder dengan diameter sekitar 3 cm dan tinggi 5 cm. Variasi parameter pembriketan yang digunakan adalah ukuran butir 20, 40 dan 80 mesh, kadar air 15 %, 20 % dan 25 %, serta suhu pembriketan sebesar 60 oC, 80 oC, 100 oC dan 120 oC. Uji pembakaran dilakukan dalam tungku berbentuk tabung horisontal berdiameter dalam 170 mm. Variasi perameter uji pembakaran yang digunakan adalah kecepatan aliran udara sebesar 0,6 m/s; 0,8 m/s; 1,0 m/s dan 1,2 m/s serta variasi ukuran butir sebesar 20, 40, dan 80 mesh. Suhu pembriketan berpengaruh signifikan terhadap peningkatan kuat tekan briket. Dari hasil uji pembakaran dapat ditentukan besarnya laju pembakaran, profil suhu pembakaran, nilai energi aktivasi (E ), konstanta Arrhenius (A), dan emisi CO. Dari semua percobaan, kadar emisi CO puncak lebih dari 400 ppm. Kata kunci: kuat tekan, daun cengkeh, jerami, bahan pengikat, ukuran butir, suhu pembriketan, kadar air, laju pembakaran, energi aktivasi, emisi CO.

Higher Derivative Corrections to Holographic Entanglement Entropy for AdS Solitons

We investigate the behaviors of holographic entanglement entropy for AdS soliton geometries in the presence of higher derivative corrections. We calculate the leading higher derivative corrections for the AdS5 setup in type IIB string and for the AdS4,7 ones in M-theory. We also study the holographic entanglement entropy in Gauss-Bonnet gravity and study how the confinement/deconfinement phase transition observed in AdS solitons is affected by the higher derivative corrections.Comment: 1+25 pages, 12 figures, LaTeX; v2: footnotes and references adde