IS FUNCTIONAL OUTCOME BETTER AFTER ARTHROPLASTY FOR TROCHANTERIC FRACTURES IN OLDER ADULTS?

Objectives: This article evaluated functional recovery and mortality after surgery to repair trochanteric fracture with regard to treatment technique through one year of follow-up. Method: Eighty consecutive patients with trochanteric fractures were divided into two groups according to treatment technique (osteosynthesis and arthroplasty). We evaluated patient data including age, sex, time to surgery, total hospital stay, transfusion volume, and functional status according to FIM (Functional Independence Measure) scores. Scores for FIM were assessed three times: prior to fracture, six months after surgery, and one year after surgery. Results: Patients who received osteosynthesis had shorter hospital stays than arthroplasty patients. The arthroplasty group had significantly higher functional independence six months after surgery, while no difference was detected one year after surgery. Patient age, transfusion volume, and FIM scores were detected as significant predictors of mortality. Conclusion: Trochanteric fractures lead to unavoidable functional loss, although this can be reduced in the short term by treating with arthroplasty instead of osteosynthesis. Age, transfusion and functional situation predict one-year mortality for patients with trochanteric fractures. The patient's functional situation must be considered when choosing treatment for trochanteric fractures in order to reduce patient morbidity

N-acetylcysteine suppresses colistimethate sodium-induced nephrotoxicity via activation of SOD2, eNOS, and MMP3 protein expressions

Objective: To investigate the molecular mechanisms of colistimethate sodium-induced nephrotoxicity and the protective effect of N-acetylcysteine (NAC) against nephrotoxicity. Methods: Twenty-eight Wistar rats were divided into four groups comprised of control, colistin, NAC, and colistin–NAC co-treatment, respectively. Serum creatinine and urine N-acetyl-β-d-glucosaminidase (NAG) levels were measured at different time intervals. Histological changes, apoptosis, total oxidant and antioxidant status, and the expression levels of endothelial nitric oxide synthase (eNOS), superoxide dismutase 2 (SOD2), and matrix metalloproteinase 3 (MMP3) were evaluated in renal tissue. Results: In the colistin group, post-treatment creatinine levels were higher than pretreatment levels (p = .001). There was a significant increase in urine NAG level following colistin treatment on day 10, compared to the baseline value and the first day of treatment (p = .001 and .0001, respectively). Urine NAG levels were higher in the colistin group on the 10th day of treatment than in the other groups (p < .01). Colistin treatment increased the apoptosis index and renal histological damage score (RHDS) significantly and these changes were reversed in NAC co-treatment (RHSD and apoptosis index were 45 and 0 for sterile saline group, 29 and 2 for NAC group, 122 and 7 for colistin group, and 66 and 2 for colistin + NAC group). We observed no difference between groups regarding total antioxidant and total oxidant status in the kidneys. The expression levels of eNOS, SOD2, and MMP3 decreased significantly in the kidneys of colistin-treated rats; these changes were reversed in the kidneys of NAC co-treated rats. Conclusions: N-acetylcysteine prevented colistin-induced nephrotoxicity through activation of expression levels of SOD2, eNOS, and MMP3

Bioactive compounds and physical attributes of Cornus mas genotypes through multivariate approaches

Cornelian cherry fruits are quite rich in bioactive compounds. Natural colour, rich flavonoids and anthocyanins and high antioxidant activity have made the fruits a natural drug. In the present study, antioxidant activity, total flavonoids and total phenolics of naturally growing 18 cornelian cherry genotypes with different phenotypic characteristics were determined. Size and shape parameters of the genotypes were also determined with the image-processing method; sphericity, elongation and shape index were calculated and shapes of two-dimensional fruit images were compared with elliptic Fourier analysis. Antioxidant activity, total flavonoid contents and total phenolic amounts of the genotypes were varied between 55.062 and 152.420 mmol TE. kg(-1), 286.40 and 2,882.80 mg QE. kg(-1), and 2,644.80 and 12,959.00 mg GAE. kg(-1), respectively. Multivariate variance analysis conducted based on physical characteristics revealed that six genotypes were different from the others. Shape analysis with Elliptic Fourier method revealed that the majority of present cornelian cherry genotypes had an oval appearance and a small portion of them had a drop-like appearance. According to discriminant analysis and Hotelling's pair-wise comparison tests, there were five different shape groups for present genotypes. A single genotype was placed into one of these groups, thus it was determined that this genotype was totally different in shape from the others

Biochemical composition and shape-dimensional traits of rosehip genotypes

In the present study, the biochemical composition and shape and dimensional traits of 25 rosehip (Rosa canina) genotypes were investigated. The shape and dimensional traits were determined by image processing technique. Seed-propagated rosehip genotypes belonging to R. canina were collected from the natural flora of Mesudiye (Ordu) and Talas (Kayseri) districts. Antioxidant activity (39.510-72.673 mmol center dot kg(-1)), total flavonoids (287.80-1,686.20 mg quercetin equivalent (QE) center dot kg(-1)) and total phenolics (38,519.40-79,080.60 mg gallic acid equivalent center dot kg(-1)) of the genotypes exhibited large variations. Width (12.2 mm) and thickness (12.5 mm) of fruits averages were found to be close to each other. The genotypes exhibited fruit lengths between 12.0 mm and 29.5 mm. Average projected area at horizontal orientation (179.7 mm(2)) was greater than the projected area at vertical orientation (120.4 mm(2)). Sphericity average was calculated as 71.4%. According to principal component (PC) analysis, the most important dimensional traits discriminating genotypes from each other were identified as surface area, geometric mean diameter and volume. In terms of shape attributes, distinctive differences were observed in sphericity, circularity, elongation and surface closure rates (SCR) of the genotypes. According to elliptic Fourier analysis (EFA), genotypes look like a sphere. In terms of shape, there were long, spherical, flat bottomed, pointed bottomed and asymmetric-looking genotypes indicating how environment and genotype affect the fruit shape. The greatest shape variation was transverse contraction and expansion. According to the clustering analysis for shape attributes, rosehip genotypes were classified into six groups. Dendrogram, scatter plots of linear discriminant analysis and paired comparison test results put forth the shape differences of the genotype successfully

Selective in-vitro Enzymes' Inhibitory Activities of Fingerprints Compounds of Salvia Species and Molecular Docking Simulations

Recently Nutrition and Food Chemistry researches have been focused on plants and their products or their secondary metabolites having anti-alzheimer, anti-cancer, anti-aging, and antioxidant properties. Among these plants Salvia L. (Lamiaceae) species come into prominence with their booster effects due to high antioxidant contents, which have over 900 species in the world and 98 in Turkey. Some Salvia species are already in use as herbal treatment of vessel stiffness, Dementia like problems and cancer. Recently some species of Salvia are of extensive research topic. In this study, inhibitory potentials of secondary metabolites, rosmarinic acid, salvigenin, salvianolic acid A and B, tanshinone I and IIA, cyrtotanshinone, dihydrotanshinone I, carnosic acid, carnosol, and danshensu sodium salt were investigated against acetylcholinesterase, butyrylcholinesterase, urease and tyrosinase enzymes both in-vitro and in slico in detail. Elevated inhibitory effects on acetyl-and butyryl-cholinesterase of dihydrotanshinone I (IC50: 1.50 +/- 0.02 and 0.50 +/- 0.01 mu g/mL, respectively), carnasol (IC50: 11.15 +/- 0.05 ve 3.92 +/- 0.03 mu g/mL) and carnosic acid (IC50: 31.83 +/- 0.65 ve 4.12 +/- 0.04 mu g/mL) were observed. Furthermore, all other secondary metabolites were active against butyrylcholinesterase. Anti-urease (42.41 +/- 0.85%) and anti-tyrosinase (39.82 +/- 1.16%) activities of tanshinone I were also observed. Potential inhibitory effects of these molecules on target proteins were investigated using DOCK and molecular dynamics calculations. Dock score analysis and Lipinski parameters were demonstrated that these ligands are potential inhibitors against relevant enzymes. Our findings suggest that Salvia species can be utilized as a ptential source of anti-alzheimer active compounds for designing novel products