A global review of marine turtle entanglement in anthropogenic debris: a baseline for further action

Entanglement in anthropogenic debris poses a threat to marine wildlife. Although this is recognised as a cause of marine turtle mortality, there remain quantitative knowledge gaps on entanglement rates and population implications. We provide a global summary of this issue in this taxon using a mixed methods approach, including a literature review and expert opinions from conservation scientists and practitioners worldwide. The literature review yielded 23 reports of marine turtle entanglement in anthropogenic debris, which included records for 6 species, in all ocean basins. Our experts reported the occurrence of marine turtles found entangled across all species, life stages and ocean basins, with suggestions of particular vulnerability in pelagic juvenile life stages. Numbers of stranded turtles encountered by our 106 respondents were in the thousands per year, with 5.5% of turtles encountered entangled; 90.6% of these dead. Of our experts questioned, 84% consider that this issue could be causing population level effects in some areas. Lost or discarded fishing materials, known as ‘ghost gear’, contributed to the majority of reported entanglements with debris from land-based sources in the distinct minority. Surveyed experts rated entanglement a greater threat to marine turtles than oil pollution, climate change and direct exploitation but less of a threat than plastic ingestion and fisheries bycatch. The challenges, research needs and priority actions facing marine turtle entanglement are discussed as pathways to begin to resolve and further understand the issue. Collaboration among stakeholder groups such as strandings networks, the fisheries sector and the scientific community will facilitate the development of mitigating actions

A [4Fe-4S]-Fe(CO)(CN)-L-cysteine intermediate is the first organometallic precursor in [FeFe] hydrogenase H-cluster bioassembly.

Biosynthesis of the [FeFe] hydrogenase active site (the 'H-cluster') requires the interplay of multiple proteins and small molecules. Among them, the radical S-adenosylmethionine enzyme HydG, a tyrosine lyase, has been proposed to generate a complex that contains an Fe(CO)2(CN) moiety that is eventually incorporated into the H-cluster. Here we describe the characterization of an intermediate in the HydG reaction: a [4Fe-4S][(Cys)Fe(CO)(CN)] species, 'Complex A', in which a CO, a CN- and a cysteine (Cys) molecule bind to the unique 'dangler' Fe site of the auxiliary [5Fe-4S] cluster of HydG. The identification of this intermediate-the first organometallic precursor to the H-cluster-validates the previously hypothesized HydG reaction cycle and provides a basis for elucidating the biosynthetic origin of other moieties of the H-cluster

Fast and flexible selection with a single switch

Selection methods that require only a single-switch input, such as a button click or blink, are potentially useful for individuals with motor impairments, mobile technology users, and individuals wishing to transmit information securely. We present a single-switch selection method, "Nomon," that is general and efficient. Existing single-switch selection methods require selectable options to be arranged in ways that limit potential applications. By contrast, traditional operating systems, web browsers, and free-form applications (such as drawing) place options at arbitrary points on the screen. Nomon, however, has the flexibility to select any point on a screen. Nomon adapts automatically to an individual's clicking ability; it allows a person who clicks precisely to make a selection quickly and allows a person who clicks imprecisely more time to make a selection without error. Nomon reaps gains in information rate by allowing the specification of beliefs (priors) about option selection probabilities and by avoiding tree-based selection schemes in favor of direct (posterior) inference. We have developed both a Nomon-based writing application and a drawing application. To evaluate Nomon's performance, we compared the writing application with a popular existing method for single-switch writing (row-column scanning). Novice users wrote 35% faster with the Nomon interface than with the scanning interface. An experienced user (author TB, with > 10 hours practice) wrote at speeds of 9.3 words per minute with Nomon, using 1.2 clicks per character and making no errors in the final text.Comment: 14 pages, 5 figures, 1 table, presented at NIPS 2009 Mini-symposi

Deletion of the GABAA α2-subunit does not alter self dministration of cocaine or reinstatement of cocaine seeking

Rationale GABAA receptors containing α2-subunits are highly represented in brain areas that are involved in motivation and reward, and have been associated with addiction to several drugs, including cocaine. We have shown previously that a deletion of the α2-subunit results in an absence of sensitisation to cocaine. Objective We investigated the reinforcing properties of cocaine in GABAA α2-subunit knockout (KO) mice using an intravenous self-administration procedure. Methods α2-subunit wildtype (WT), heterozygous (HT) and KO mice were trained to lever press for a 30 % condensed milk solution. After implantation with a jugular catheter, mice were trained to lever press for cocaine (0.5 mg/kg/infusion) during ten daily sessions. Responding was extinguished and the mice tested for cue- and cocaine-primed reinstatement. Separate groups of mice were trained to respond for decreasing doses of cocaine (0.25, 0.125, 0.06 and 0.03 mg/kg). Results No differences were found in acquisition of lever pressing for milk. All genotypes acquired self-administration of cocaine and did not differ in rates of self-administration, dose dependency or reinstatement. However, whilst WT and HT mice showed a dose-dependent increase in lever pressing during the cue presentation, KO mice did not. Conclusions Despite a reported absence of sensitisation, motivation to obtain cocaine remains unchanged in KO and HT mice. Reinstatement of cocaine seeking by cocaine and cocaine-paired cues is also unaffected. We postulate that whilst not directly involved in reward perception, the α2-subunit may be involved in modulating the “energising” aspect of cocaine’s effects on reward-seeking

Plastic Pollution and Small Juvenile Marine Turtles: A Potential Evolutionary Trap

The ingestion of plastic by marine turtles is now reported for all species. Small juvenile turtles (including post-hatchling and oceanic juveniles) are thought to be most at risk, due to feeding preferences and overlap with areas of high plastic abundance. Their remote and dispersed life stage, however, results in limited access and assessments. Here, stranded and bycaught specimens from Queensland Australia, Pacific Ocean (PO;n = 65; 1993–2019) and Western Australia, Indian Ocean (IO; n = 56; 2015–2019) provide a unique opportunity to assess the extent of plastic (> 1mm) ingestion in five species [green (Chelonia mydas), loggerhead (Caretta caretta), hawksbill (Eretmochelys imbricata), olive ridley (Lepidochelys olivacea), and flatback turtles (Natator depressus)]. In the Pacific Ocean, high incidence of ingestion occurred in green (83%; n = 36), loggerhead (86%; n = 7), flatback (80%; n = 10) and olive ridley turtles (29%; n = 7). There was an overall lower incidence in IO; highest being in the flatback (28%; n = 18), the loggerhead (21%; n = 14) and green (9%; n = 22). No macroplastic debris ingestion was documented for hawksbill turtles in either site although sample sizes were smaller for this species (PO n = 5; IO n = 2). In the Pacific Ocean, the majority of ingested debris was made up of hard fragments (mean of all species 52%; species averages 46–97%), whereas for the Indian Ocean these were filamentous plastics (52%; 43–77%). The most abundant colour for both sites across all species was clear (PO: 36%; IO: 39%), followed by white for PO (36%) then green and blue for IO (16%; 16%). The polymers most commonly ingested by turtles in both oceans were polyethylene (PE; PO-58%; IO-39%) and polypropylene (PP; PO-20.2%; IO-23.5%). We frame the high occurrence of ingested plastic present in this marine turtle life stage as a potential evolutionary trap as they undertake their development in what are now some of the most polluted areas of the global oceans

Dietary analysis of two sympatric marine turtle species in the eastern Mediterranean

This is the final version. Available from Springer via the DOI in this record. Data and any available visuals, such as figures and tables, will be provided upon reasonable request to the corresponding author. Any codes created to assist in data analysis and/or any software application utilised in the current study will be provided upon reasonable request submitted to the corresponding author.Dietary studies provide key insights into threats and changes within ecosystems and subsequent impacts on focal species. Diet is particularly challenging to study within marine environments and therefore is often poorly understood. Here, we examined the diet of stranded and bycaught loggerhead (Caretta caretta) and green turtles (Chelonia mydas) in North Cyprus (35.33° N, 33.47° E) between 2011 and 2019. A total of 129 taxa were recorded in the diet of loggerhead turtles (n = 45), which were predominantly carnivorous (on average 72.1% of dietary biomass), foraging on a large variety of invertebrates, macroalgae, seagrasses and bony fish in low frequencies. Despite this opportunistic foraging strategy, one species was particularly dominant, the sponge Chondrosia reniformis (21.5%). Consumption of this sponge decreased with increasing turtle size. A greater degree of herbivory was found in green turtles (n = 40) which predominantly consumed seagrasses and macroalgae (88.8%) with a total of 101 taxa recorded. The most dominant species was a Lessepsian invasive seagrass, Halophila stipulacea (31.1%). This is the highest percentage recorded for this species in green turtle diet in the Mediterranean thus far. With increasing turtle size, the percentage of seagrass consumed increased with a concomitant decrease in macroalgae. Seagrass was consumed year-round. Omnivory occurred in all green turtle size classes but reduced in larger turtles (> 75 cm CCL) suggesting a slow ontogenetic dietary shift. Macroplastic ingestion was more common in green (31.6% of individuals) than loggerhead turtles (5.7%). This study provides the most complete dietary list for marine turtles in the eastern Mediterranean.European CommissionErwin Warth FoundationKarşıyaka Turtle WatchAngela WadsworthMaureen and Tony HutchinsonKuzey Kıbrıs TurkcellMAVA FoundationNatural Environment Research Council (NERC)University of Exete

Diet-related selectivity of macroplastic ingestion in green turtles (Chelonia mydas) in the eastern Mediterranean

Understanding the drivers of key interactions between marine vertebrates and plastic pollution is now considered a research priority. Sea turtles are primarily visual predators, with the ability to discriminate according to colour and shape; therefore these factors play a role in feeding choices. Classifcation methodologies of ingested plastic currently do not record these variables, however here, refned protocols allow us to test the hypothesis that plastic is selectively ingested when it resembles the food items of green turtles (Chelonia mydas). Turtles in the eastern Mediterranean displayed strong diet-related selectivity towards certain types (sheet and threadlike), colours (black, clear and green) and shapes (linear items strongly preferred) of plastic when compared to the environmental baseline of plastic beach debris. There was a signifcant negative relationship between size of turtle (curved carapace length) and number/mass of plastic pieces ingested, which may be explained through naivety and/or ontogenetic shifts in diet. Further investigation in other species and sites are needed to more fully ascertain the role of selectivity in plastic ingestion in this marine vertebrate group

Feasibility of MR-Based Body Composition Analysis in Large Scale Population Studies

Introduction Quantitative and accurate measurements of fat and muscle in the body are important for prevention and diagnosis of diseases related to obesity and muscle degeneration. Manually segmenting muscle and fat compartments in MR body-images is laborious and time-consuming, hindering implementation in large cohorts. In the present study, the feasibility and success-rate of a Dixon-based MR scan followed by an intensity-normalised, non-rigid, multi-atlas based segmentation was investigated in a cohort of 3,000 subjects. Materials and Methods 3,000 participants in the in-depth phenotyping arm of the UK Biobank imaging study underwent a comprehensive MR examination. All subjects were scanned using a 1.5 T MR-scanner with the dual-echo Dixon Vibe protocol, covering neck to knees. Subjects were scanned with six slabs in supine position, without localizer. Automated body composition analysis was performed using the AMRA Profiler™ system, to segment and quantify visceral adipose tissue (VAT), abdominal subcutaneous adipose tissue (ASAT) and thigh muscles. Technical quality assurance was performed and a standard set of acceptance/rejection criteria was established. Descriptive statistics were calculated for all volume measurements and quality assurance metrics. Results Of the 3,000 subjects, 2,995 (99.83%) were analysable for body fat, 2,828 (94.27%) were analysable when body fat and one thigh was included, and 2,775 (92.50%) were fully analysable for body fat and both thigh muscles. Reasons for not being able to analyse datasets were mainly due to missing slabs in the acquisition, or patient positioned so that large parts of the volume was outside of the field-of-view. Discussion and Conclusions In conclusion, this study showed that the rapid UK Biobank MR-protocol was well tolerated by most subjects and sufficiently robust to achieve very high success-rate for body composition analysis. This research has been conducted using the UK Biobank Resource

Mindfulness in schools: a health promotion approach to improving adolescent mental health.

Between 10 and 20% of adolescents worldwide experience a mental health problem within a given 12-month period. Mental health problems impact on an adolescent’s potential to live a fulfilling and productive life and lead to challenges such as stigma, isolation and discrimination. To address this need, in recent years, there has been growing interest into broad-based school-integrated health promotion interventions that seek to build resilience and augment protective factors in adolescents. Mindfulness-based interventions (MBIs) reflect one such approach that have been administered to adolescent populations in both resilience building and treatment contexts. This paper discusses the utility of school-based MBIs as an adolescent health promotion approach and makes recommendations for intervention design, delivery and evaluation. Emerging evidence indicates that school-integrated MBIs may be a cost-effective means of not only meeting government objectives relating to adolescent mental health, but also for improving the wellbeing of teachers and parents. Furthermore, there is growing evidence indicating that mindfulness can elicit improvements in student learning performance and general classroom behaviour. However, notwithstanding these beneficial properties, there remains a need to conduct large-scale empirical investigations that seek to evaluate the effectiveness of school-integrated MBIs at a regional or national level. A further challenge is the need to ensure that mindfulness instructors are able to impart to adolescents an experiential understanding of this ancient contemplative technique.N