PI3 K/Akt/mTOR-mediated translational control regulates proliferation and differentiation of lineage-restricted RoSH stem cell lines

Background: We have previously derived highly similar lineage-restricted stem cell lines, RoSH and E-RoSH cell lines from mouse embryos and CD9hi SSEA-1- differentiated mouse embryonic stem cells, respectively. These cell lines are not pluripotent and differentiate readily into endothelial cells in vitro and in vivo. Results: We investigated the signaling pathway that maintains proliferation of these cells in an undifferentiated state, and demonstrate that PI3 K/Akt/mTOR, but not Raf/MEK/Erk, signaling in these cells was active during proliferation and was downregulated during endothelial differentiation. Inhibition of PI3 K/Akt/mTOR signaling, but not Raf/MEK/Erk, reduced proliferation and induced expression of endothelial specific proteins. During differentiation or inhibition of PI3 K/Akt/mTOR signaling, cyclinD2 transcript abundance in ribosome-enriched RNA but not in total RNA was reduced with a corresponding reduction in protein level. In contrast, transcript abundance of endothelial-specific genes e.g. Kdr, Tek and Pdgfrα in ribosome-enriched RNA fraction was not reduced and their protein levels were increased. Together these observations suggested that translational control mediated by PI3K/Akt/mTOR signaling was critical in regulating proliferation and endothelial differentiation of lineage-restricted RoSH-like stem cell lines. Conclusion: This study highlights translation regulation as a critical regulatory mechanism during proliferation and differentiation in stem cells

Elogio histórico del brigadier de la Real Armada Don Cosme Damián de Churruca y Elorza, que murió en el combate de Trafalgar en 21 de octubre de 1805

Digitalización. Vitoria-Gasteiz : Archivos y Bibliotecas, Abril 199

The People Living With HIV Stigma Index: Dominican Republic

The People Living with HIV Stigma Index, developed in 2008, is a survey instrument used to highlight stigma and discrimination experienced by PLHIV. Developed and administered by and for PLHIV, the Stigma Index is not merely a measurement tool, its implementation is intended to be an empowering experience for PLHIV. In 2016–17, the Population Council–led research consortium Project SOAR updated the Stigma Index to reflect changes in the global HIV response in the past decade. The new version (Stigma Index 2.0) now has an increased focus on PLHIV’s experiences accessing HIV services; using and adhering to antiretroviral services; mental health and resilience; and stigma within health-care settings. Implementation of Stigma Index 2.0 in the Dominican Republic (DR) was led by PLHIV. Findings update results from the original Stigma Index fielded in the DR more than a decade ago. With the inclusion of modules on mental health, expanded content on experiences in health-care settings, and intentional recruitment of MSM, FSWs, and PLHIV of Haitian descent, Stigma Index 2.0 sheds light on ongoing challenges and opportunities regarding HIV-related stigma in the DR, as detailed in this report

Título: Por el libro de la muger fuerte, Doña Maria Vela, respondiendo a las dudas que se han puesto en èl y en el espiritu y vida de la Santa

Copia digital. Valladolid : Junta de Castilla y León. Consejería de Cultura y Turismo, 2009-2010Marca de ed. en ambas port.Texto a dos col. con apostillas marginalesHojas escritas por ambas carasSign.: [calderón]8, 2[calderón]2,A-Z8, 2A-H8, 2I4Esc. xil.Del maestro Fray Angel Manrique ... Por el libro de la muger fuerte, Doña Maria Vela respondiendo a las dudas que se han puesto en el y en el espiritu y vida de la Santa : p. 198-248, con port. propi

Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings

We report a one-pot synthesis of spherical gold nanoparticles (52-22 nm) and their capping with cefaclor, a second-generation antibiotic, without use of other chemicals. The differently sized gold nanoparticles were fabricated by controlling the rate of reduction of gold ions in aqueous solution by varying the reaction temperature (20-70 C). The primary amine group of cefaclor acted as both the reducing and capping agent for the synthesis of gold nanoparticles leaving the b-lactam ring of cefaclor available for activity against microbes. Antimicrobial testing showed that cefaclor reduced gold nanoparticles have potent antimicrobial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria as compared to cefaclor or gold nanoparticles alone. The minimum inhibition concentrations (MICs) of cefaclor reduced gold nanoparticles were 10m gmL1 and 100m gmL1 for S. aureus and E. coli respectively. The cefaclor reduced gold nanoparticles were further coated onto poly(ethyleneimine) (PEI) modified glass surfaces to obtain antimicrobial coatings suitable for biomedical applications and were tested against E. coli as an exemplar of activity. The antimicrobial coatings were very robust under adverse conditions (pH 3 and 10), inhibited the growth of E. coli on their surfaces, and could be used many times with retained activity. Results from a combined spectroscopic (FTIR) and microscopic study (AFM) suggest that the action of these novel particles is through the combined action of cefaclor inhibiting the synthesis of the peptidoglycan layer and gold nanoparticles generating "holes" in bacterial cell walls thereby increasing the permeability of the cell wall, resulting in the leakage of cell contents and eventually cell death

Protein trafficking through the endosomal system prepares intracellular parasites for a home invasion

Toxoplasma (toxoplasmosis) and Plasmodium (malaria) use unique secretory organelles for migration, cell invasion, manipulation of host cell functions, and cell egress. In particular, the apical secretory micronemes and rhoptries of apicomplexan parasites are essential for successful host infection. New findings reveal that the contents of these organelles, which are transported through the endoplasmic reticulum (ER) and Golgi, also require the parasite endosome-like system to access their respective organelles. In this review, we discuss recent findings that demonstrate that these parasites reduced their endosomal system and modified classical regulators of this pathway for the biogenesis of apical organelles

Essential Facts on the History of Hyperthermia and their Connections with Electromedicine

The term hyperthermia is a combination of two Greek words: HYPER (rise) and THERME (heat) and refers to the increasing of body temperature or selected tissues in order to achieve a precise therapeutic effect. This paper reviews the development of thermotherapy by describing the most important moments in its history. For decades, the development of hyperthermia ran parallel with the development of cancer treatment and had numerous connections with electromedicine. Throughout its history, hyperthermia evoked a number of hopes, brought spectacular successes, but also was the subject of many disappointments

Evidence That SOX2 Overexpression Is Oncogenic in the Lung

BACKGROUND: SOX2 (Sry-box 2) is required to maintain a variety of stem cells, is overexpressed in some solid tumors, and is expressed in epithelial cells of the lung. METHODOLOGY/PRINCIPAL FINDINGS: We show that SOX2 is overexpressed in human squamous cell lung tumors and some adenocarcinomas. We have generated mouse models in which Sox2 is upregulated in epithelial cells of the lung during development and in the adult. In both cases, overexpression leads to extensive hyperplasia. In the terminal bronchioles, a trachea-like pseudostratified epithelium develops with p63-positive cells underlying columnar cells. Over 12-34 weeks, about half of the mice expressing the highest levels of Sox2 develop carcinoma. These tumors resemble adenocarcinoma but express the squamous marker, Trp63 (p63). CONCLUSIONS: These findings demonstrate that Sox2 overexpression both induces a proximal phenotype in the distal airways/alveoli and leads to cancer

Tomo lo que encuentro : 19 versiones de Virus

“No hay que juzgar a un producto argentino por lo que le falta con respecto a uno americano o europeo... La crítica suele ser más permisiva con las producciones extranjeras que con las locales... No quiero que tengamos una fama tan grande que nos impida la libertad de seguir haciendo lo que querramos”. Tres conceptos de Federico Moura en el último reportaje radial que le hice (y que se le hizo), que bastarían para mostrar a alguien atento a lo que pasaba y que tenía las palabras para dar cuenta de ese registro. Esto está presente en toda la obra de Virus. Una producción que siempre fue creciendo en calidad, en forma y contenido. Este CD es el mejor testimonio de su vigencia. Que tantos solistas y grupos platenses, talentosos y apasionados, hayan tenido deseos de hacer su versión de temas de Virus, es el reconocimiento más pleno de una obra genuina, que despierta amor y respeto. Y claro que Federico está. Si no cómo sabríamos que falta. Tom Lupo Contenido: 1 - Sergio Pángaro: "Tomo lo que encuentro" 2 - Míster América: "Dame una señal" 3 - Ex: "El banquete" 4 - Cine Continuado: "Wadu-wadu" 5 - Ahora: "Sin disfraz" 6 - Las Canoplas: "En mi garage" 7 - Federico Kempff: "Desesperado secuencia uno" 8 - Alfredo Calvelo: "Loco coco" 9 - Traviatabosnialafortina: "Juegos postergados" 10 - Estelares: "Pronta entrega" 11 - Falso Primer Ministro: "Danza narcótica" 12 - NerdKids: "Me puedo programar" 13 - Plupart: "Se sarpo" 14 - Francisco Bochatón: "Imágenes paganas" 15 - El César: "El 146" 16 - Los Hermanitos Kaiser y su Increíble Organito que Toca Solo: "Juegos incompletos" 17 - La Secta: "Mirada speed" 18 - Norma: "Densa realidad" 19 - Juan Pablo Bochatón: "Tomo lo que encuentro"Radio Universidad Nacional de La Plat