\u3cem\u3ePlasmodium falciparum\u3c/em\u3e SSB Tetramer Binds Single-Stranded DNA Only in a Fully Wrapped Mode

The tetrameric Escherichia coli single-stranded DNA (ssDNA) binding protein (Ec-SSB) functions in DNA metabolism by binding to ssDNA and interacting directly with numerous DNA repair and replication proteins. Ec-SSB tetramers can bind ssDNA in multiple DNA binding modes that differ in the extent of ssDNA wrapping. Here, we show that the structurally similar SSB protein from the malarial parasite Plasmodium falciparum (Pf-SSB) also binds tightly to ssDNA but does not display the same number of ssDNA binding modes as Ec-SSB, binding ssDNA exclusively in fully wrapped complexes with site sizes of 52–65 nt/tetramer. Pf-SSB does not transition to the more cooperative (SSB)35 DNA binding mode observed for Ec-SSB. Consistent with this, Pf-SSB tetramers also do not display the dramatic intra-tetramer negative cooperativity for binding of a second (dT)35 molecule that is evident in Ec-SSB. These findings highlight variations in the DNA binding properties of these two highly conserved homotetrameric SSB proteins, and these differences might be tailored to suit their specific functions in the cell

A review of the population structure of yellowfin tuna, Thunnus albacares, in the Eastern Pacific Ocean

ENGLISH: Since its inception in 1950 by agreement between the Republic of Costa Rica and the United States of America, the Inter-American Tropical Tuna Commission has been engaged in studies of the biology, ecology and population dynamics of yellowfin tuna in the eastern Pacific Ocean. Prime consideration has been given to the evaluation of the effects of fishing pressure on the yellowfin tuna in this area in order to estimate the maximum sustainable yield. A portion of the eastern Pacific has been defined by the Inter-American Tropical Tuna Commission (1963) as a regulatory area for yellowfin tuna (Figure 1). SPANISH: Desde su incepción en 1950, por un acuerdo entre la República de Costa Rica y los Estados Unidos de América, la Comisión Interamericana del Atún Tropical ha estado ocupada en los estudios de la biología, ecología y dinámica de las poblaciones del atún aleta amarilla en el Océano Pacífico Oriental. Se consideró primariamente la evaluación de los efectos de la presión de la pesquería sobre el atún aleta amarilla en esta área, para poder estimar el rendimiento máximo sostenible. Una parte del Pacífico Oriental ha sido definida por la Comisión Interamericana del Atún Tropical (1963), como área de reglamentación del atún aleta amarilla (Figura 1). (PDF contains 60 pages.

Science requirements for a global change technology architecture trade study

Science requirements for a global change technology initiative (GCTI) Architecture Trade Study were established by reviewing and synthesizing results from recent studies. A scientific rationale was adopted and used to identify a comprehensive set of measureables and their priorities. Spatial and temporal requirements for a number of measurement parameters were evaluated based on results from several working group studies. Science requirements were defined using these study results in conjunction with the guidelines for investigating global changes over a time scale of decades to centuries. Requirements are given separately for global studies and regional process studies. For global studies, temporal requirements are for sampling every 1 to 12 hours for atmospheric and radiation parameters and 1 day or more for most earth surface measurements. Therefore, the atmospheric measureables provide the most critical drivers for temporal sampling. Spatial sampling requirements vary from 1 km for land and ocean surface characteristics to 50 km for some atmospheric parameters. Thus, the land and ocean surface parameters have the more significant spatial variations and provide the most challenging spatial sampling requirements

Blue horizontal branch stars in the Sloan Digital Sky Survey: II. Kinematics of the Galactic halo

We carry out a maximum-likelihood kinematic analysis of a sample of 1170 blue horizontal branch (BHB) stars from the Sloan Digital Sky Survey presented in Sirko et al. (2003) (Paper I). Monte Carlo simulations and resampling show that the results are robust to distance and velocity errors at least as large as the estimated errors from Paper I. The best-fit velocities of the Sun (circular) and halo (rotational) are 245.9 +/- 13.5 km/s and 23.8 +/- 20.1 km/s but are strongly covariant, so that v_0 - v_halo = 222.1 +/- 7.7 km/s. If one adopts standard values for the local standard of rest and solar motion, then the halo scarcely rotates. The velocity ellipsoid inferred for our sample is much more isotropic [(sigma_r,sigma_theta,sigma_phi) = (101.4 +/- 2.8, 97.7 +/- 16.4, 107.4 +/- 16.6) km/s] than that of halo stars in the solar neighborhood, in agreement with a recent study of the distant halo by Sommer-Larsen et al. (1997). The line-of-sight velocity distribution of the entire sample, corrected for the Sun's motion, is accurately gaussian with a dispersion of 101.6 +/- 3.0 km/s.Comment: 23 pages including 4 figures, 1 color; submitted to A

Aligning Mentorship Style With Beginning Teachers' Development: Contextual Supervision

Contextual Supervision (CS), a developmental mentoring model recently designed, applied, and refined in a teaching internship setting in Western Canada, has been shown to be useful in providing guidance to the supervision of teacher-interns during their extended practicum. The present study (in which the author was a participant observer) provides further qualitative and quantitative evidence that illustrates the effectiveness of CS in guiding practicum supervisors in the task of helping beginning teachers to develop their instructional skills. It also suggests that the CS model can prove useful in supervisory practice with developing practitioners in a variety of instructional settings.La supervision contextuelle (Contextual Supervision - CS, en anglais), un modèle de mentorat expérimental que l'on vient de concevoir, appliquer et raffiner pendant un stage pédagogique dans l'Ouest du Canada, s'est avérée utile pour guider la supervision de stagiaires pendant leur stage prolongé. Cette étude (lors de laquelle l'auteur a agit tant comme participant que comme observateur) ajoute aux preuves qualitatives et quantitatives qui démontrent l'efficacité de la supervision contextuelle dans l'encadrement des superviseurs de stagiaires pendant qu'ils les aident dans l'acquisition d'habiletés pédagogiques. De plus, les résultats laissent croire que ce modèle pourrait être utile lors de la supervision de stagiaires dans différents milieux d'enseignement